AIM： To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. METHODS： A total of 54 patients were divided i...AIM： To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. METHODS： A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy： normal control group （25 patients） and gastric cancer group （29 patients）. Both groups were further divided into Hpylori （＋） and H pylori （-） subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique （HelicoBIot 2.0, Genelabs Diagnostics, Singapore）. RESULTS： The positive rate of the immunoblotting test was as high as 88.9% in the H pylori （-） gastric cancer group and only 14.3% in the H pylori （-） normal control group with a statistically significant difference. CONCLUSION： The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.展开更多
Objective: To comprehensively understand the infection status of human T-lymphotropic virus (HTLV) among the voluntary blood donors in Yulin City and accurately assess the impact of HTLV on the current situation of bl...Objective: To comprehensively understand the infection status of human T-lymphotropic virus (HTLV) among the voluntary blood donors in Yulin City and accurately assess the impact of HTLV on the current situation of blood safety in Yulin City. Methods: A total of 113,588 blood samples from voluntary blood donors collected in Yulin City from January 2023 to June 2024 were selected. The HTLV-I/II antibody screening was carried out on these samples using the HTLV enzyme-linked immunosorbent assay (ELISA) kit. For the samples with reactive screening results, further confirmation was performed by means of immunoblotting assay and real-time fluorescent quantitative polymerase chain reaction (qPCR). Results: Among the 113,588 blood samples from voluntary blood donors, 20 samples showed positive reactions for HTLV-I/II antibodies, with an initial screening positive reaction rate of 1.76‱. After confirmation, 8 of them were confirmed to be HTLV-I positive, with a positive rate of 0.7‱, and no HTLV-II positive blood donors were detected. Moreover, all the HTLV-I positive blood donors were Han people from Yulin. In addition, by following up the 4 children of a female positive blood donor, it was found that her eldest daughter was confirmed to be HTLV-I positive, while the test results of the other children were negative. Conclusion: At present, there is no HTLV-II infection among the voluntary blood donors in Yulin City. There is a relatively low level of HTLV-I infection mainly among the local people. Continuous monitoring is still needed in the future. Emphasis should be placed on strengthening the publicity, education and testing work for the close relatives of the confirmed positive blood donors and women during pregnancy and childbirth periods, so as to prevent and reduce the HTLV infection caused by family transmission.展开更多
目的确定丙型肝炎病毒(hepatitis C virus,HCV)抗体阳性预测值≥95%的最佳吸光度/临界值(signal-to-cutoff,S/CO),确定首都医科大学附属北京地坛医院实验室医学决定水平,为探索不同系统丙型肝炎病毒抗体检测试剂95%阳性置信区间建立方...目的确定丙型肝炎病毒(hepatitis C virus,HCV)抗体阳性预测值≥95%的最佳吸光度/临界值(signal-to-cutoff,S/CO),确定首都医科大学附属北京地坛医院实验室医学决定水平,为探索不同系统丙型肝炎病毒抗体检测试剂95%阳性置信区间建立方法学。方法收集2021年7月至2022年2月门诊及住院患者进行化学发光免疫分析法(chemiluminescence analysis,CLIA)初筛检测同时进行HCV RNA检测的血浆样本共282例,其中抗体初筛有反应性(S/CO≥1)样本252例,阴性样本30例,进行重组免疫印记试验(recombinant immunoblot assay,RIBA)并查阅其HCV RNA检测结果,通过受试者工作特征(receiver operating characteristic,ROC)曲线得到预测本实验室阳性预测值≥95%时HCV感染的抗体S/CO值。结果初筛为阴性的30例样本双重确证试验结果均为阴性;排除16例HCV感染史不明确的样本,丙型肝炎病毒抗体S/CO≥1的236例样本经双重确证试验得到真阳性样本188例,阴性样本48例,经ROC曲线分析,本实验室阳性预测值≥95%时,本实验室抗体S/CO值为7.83,灵敏度为93.09%,特异度为95.83%,曲线下面积(area under the curve,AUC)为0.98(P<0.0001)。结论为临床提供本实验室阳性预测值≥95%时HCV感染抗体的S/CO值以协助临床诊疗。展开更多
文摘AIM： To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. METHODS： A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy： normal control group （25 patients） and gastric cancer group （29 patients）. Both groups were further divided into Hpylori （＋） and H pylori （-） subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique （HelicoBIot 2.0, Genelabs Diagnostics, Singapore）. RESULTS： The positive rate of the immunoblotting test was as high as 88.9% in the H pylori （-） gastric cancer group and only 14.3% in the H pylori （-） normal control group with a statistically significant difference. CONCLUSION： The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.
文摘Objective: To comprehensively understand the infection status of human T-lymphotropic virus (HTLV) among the voluntary blood donors in Yulin City and accurately assess the impact of HTLV on the current situation of blood safety in Yulin City. Methods: A total of 113,588 blood samples from voluntary blood donors collected in Yulin City from January 2023 to June 2024 were selected. The HTLV-I/II antibody screening was carried out on these samples using the HTLV enzyme-linked immunosorbent assay (ELISA) kit. For the samples with reactive screening results, further confirmation was performed by means of immunoblotting assay and real-time fluorescent quantitative polymerase chain reaction (qPCR). Results: Among the 113,588 blood samples from voluntary blood donors, 20 samples showed positive reactions for HTLV-I/II antibodies, with an initial screening positive reaction rate of 1.76‱. After confirmation, 8 of them were confirmed to be HTLV-I positive, with a positive rate of 0.7‱, and no HTLV-II positive blood donors were detected. Moreover, all the HTLV-I positive blood donors were Han people from Yulin. In addition, by following up the 4 children of a female positive blood donor, it was found that her eldest daughter was confirmed to be HTLV-I positive, while the test results of the other children were negative. Conclusion: At present, there is no HTLV-II infection among the voluntary blood donors in Yulin City. There is a relatively low level of HTLV-I infection mainly among the local people. Continuous monitoring is still needed in the future. Emphasis should be placed on strengthening the publicity, education and testing work for the close relatives of the confirmed positive blood donors and women during pregnancy and childbirth periods, so as to prevent and reduce the HTLV infection caused by family transmission.
文摘目的确定丙型肝炎病毒(hepatitis C virus,HCV)抗体阳性预测值≥95%的最佳吸光度/临界值(signal-to-cutoff,S/CO),确定首都医科大学附属北京地坛医院实验室医学决定水平,为探索不同系统丙型肝炎病毒抗体检测试剂95%阳性置信区间建立方法学。方法收集2021年7月至2022年2月门诊及住院患者进行化学发光免疫分析法(chemiluminescence analysis,CLIA)初筛检测同时进行HCV RNA检测的血浆样本共282例,其中抗体初筛有反应性(S/CO≥1)样本252例,阴性样本30例,进行重组免疫印记试验(recombinant immunoblot assay,RIBA)并查阅其HCV RNA检测结果,通过受试者工作特征(receiver operating characteristic,ROC)曲线得到预测本实验室阳性预测值≥95%时HCV感染的抗体S/CO值。结果初筛为阴性的30例样本双重确证试验结果均为阴性;排除16例HCV感染史不明确的样本,丙型肝炎病毒抗体S/CO≥1的236例样本经双重确证试验得到真阳性样本188例,阴性样本48例,经ROC曲线分析,本实验室阳性预测值≥95%时,本实验室抗体S/CO值为7.83,灵敏度为93.09%,特异度为95.83%,曲线下面积(area under the curve,AUC)为0.98(P<0.0001)。结论为临床提供本实验室阳性预测值≥95%时HCV感染抗体的S/CO值以协助临床诊疗。
