AIM： To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. METHODS： A total of 54 patients were divided i...AIM： To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. METHODS： A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy： normal control group （25 patients） and gastric cancer group （29 patients）. Both groups were further divided into Hpylori （＋） and H pylori （-） subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique （HelicoBIot 2.0, Genelabs Diagnostics, Singapore）. RESULTS： The positive rate of the immunoblotting test was as high as 88.9% in the H pylori （-） gastric cancer group and only 14.3% in the H pylori （-） normal control group with a statistically significant difference. CONCLUSION： The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.展开更多
Autoimmune hepatitis(AIH)is a liver disease of unknown cause characterized by hypergammaglobulinemia,typical or compatible liver histology,the absence of viral hepatitis and the production of autoantibodies.Anti-smoot...Autoimmune hepatitis(AIH)is a liver disease of unknown cause characterized by hypergammaglobulinemia,typical or compatible liver histology,the absence of viral hepatitis and the production of autoantibodies.Anti-smooth muscle antibodies(SMAs)detected with indirect immunofluorescence(IIF)in rodent tissues are not disease specific,whereas antibodies directed to the filamentous form of actin(F-actin)are specifically involved in AIH-1.As solid-phase immunoassays(SPAs)specifically targeting F-actin,such as enzyme-linked immunosorbent assay(ELISA),have already been included in recent guidelines,in this study,we evaluated the diagnostic per-formance of an immunoblotting SPA for F-actin.We selected 86 samples positive for SMA by IIF(titre≥1:40)and/or for anti-F-actin by ELISA(≥20 units);the patients were divided into 3 groups:AIH-1(n=14),other liver disorders(n=38)and other nonliver-related conditions(n=34).The samples were tested with an immunoblot SPA(European Autoimmune Liver Diseases 9 Ag plus F-actin,Euroimmun,Germany);the qualitative results were converted into numeric intensity values via EurolineScan software.Immunoblotting revealed 16 positive samples(19%),and ELISA revealed 24(28%);among them,7/16(44%)and 11/24(46%)had AIH-1.The diagnostic performance metrics were as follows:sensitivity(50%);specificity(87.5%);positive predictive value(PPV),43.8%;negative predictive value(NPV),90%;and accuracy(81.4%)and equal to those of ELISA.The mean values were greater in the AIH-1 group;the receiver operating characteristic curve(ROC)had an area under curve(AUC)of 0.77 and was not different from that of the ELISA(0.82);the agreement was 81.4%,with a Cohens kappa of 0.49.Immunoblotting might be a reliable assay for the identification of anti-F-actin antibodies,and given its high specificity,its implementation in a clinical laboratory might confirm the specific diagnosis of AIH-1 in patients with IIF-detected SMA.展开更多
文摘AIM： To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer. METHODS： A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy： normal control group （25 patients） and gastric cancer group （29 patients）. Both groups were further divided into Hpylori （＋） and H pylori （-） subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique （HelicoBIot 2.0, Genelabs Diagnostics, Singapore）. RESULTS： The positive rate of the immunoblotting test was as high as 88.9% in the H pylori （-） gastric cancer group and only 14.3% in the H pylori （-） normal control group with a statistically significant difference. CONCLUSION： The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.
文摘Autoimmune hepatitis(AIH)is a liver disease of unknown cause characterized by hypergammaglobulinemia,typical or compatible liver histology,the absence of viral hepatitis and the production of autoantibodies.Anti-smooth muscle antibodies(SMAs)detected with indirect immunofluorescence(IIF)in rodent tissues are not disease specific,whereas antibodies directed to the filamentous form of actin(F-actin)are specifically involved in AIH-1.As solid-phase immunoassays(SPAs)specifically targeting F-actin,such as enzyme-linked immunosorbent assay(ELISA),have already been included in recent guidelines,in this study,we evaluated the diagnostic per-formance of an immunoblotting SPA for F-actin.We selected 86 samples positive for SMA by IIF(titre≥1:40)and/or for anti-F-actin by ELISA(≥20 units);the patients were divided into 3 groups:AIH-1(n=14),other liver disorders(n=38)and other nonliver-related conditions(n=34).The samples were tested with an immunoblot SPA(European Autoimmune Liver Diseases 9 Ag plus F-actin,Euroimmun,Germany);the qualitative results were converted into numeric intensity values via EurolineScan software.Immunoblotting revealed 16 positive samples(19%),and ELISA revealed 24(28%);among them,7/16(44%)and 11/24(46%)had AIH-1.The diagnostic performance metrics were as follows:sensitivity(50%);specificity(87.5%);positive predictive value(PPV),43.8%;negative predictive value(NPV),90%;and accuracy(81.4%)and equal to those of ELISA.The mean values were greater in the AIH-1 group;the receiver operating characteristic curve(ROC)had an area under curve(AUC)of 0.77 and was not different from that of the ELISA(0.82);the agreement was 81.4%,with a Cohens kappa of 0.49.Immunoblotting might be a reliable assay for the identification of anti-F-actin antibodies,and given its high specificity,its implementation in a clinical laboratory might confirm the specific diagnosis of AIH-1 in patients with IIF-detected SMA.
