Fifteen cases of fresh fractures have been studied, including 14 cases of tubu-lar bone fractures with age from 28 to 60 years and one case of vertebral fracture ofdorsolumbar region, 16 years of age. There were 13 ma...Fifteen cases of fresh fractures have been studied, including 14 cases of tubu-lar bone fractures with age from 28 to 60 years and one case of vertebral fracture ofdorsolumbar region, 16 years of age. There were 13 male and 2 female patients. Theyhad no endocrine disturbances or other bone diseases. The ratio ofhydroxyproline/creatinine (HP/Cr) in these cases increased gradually within 20-30 daysafter fracture and then reached the peak value which was about 3-4 times of normal, thepeak value maintained for a long time. If the trauma was mild or the patient was young,the peak began to decline on the 60th day after fracture. While in those severe cases withprolonged confinement to bed, the peak only began to come down about 110th dayafter fracture. The ratio of HP/Cr decreased rapidly from the peak at first, then pursueda slow course. Generally, the ratio of HP/Cr returned to normal on the 240th to 310thday. Bone union began at a time when peak value started to decline, and with completionof the remodelling process, the HP/Cr value returned to normal.展开更多
This study was conducted to investigate the effect of dietary proline(Pro), and Pro and hydroxyproline(Hyp) in combination on the growth performance, total Hyp and collagen concentrations of tissues, and prolyl 4-hydr...This study was conducted to investigate the effect of dietary proline(Pro), and Pro and hydroxyproline(Hyp) in combination on the growth performance, total Hyp and collagen concentrations of tissues, and prolyl 4-hydroxylase α(I)(P4H α(I)) gene expression in juvenile turbot feeding high plant protein diets. A diet containing 50% crude protein and 12% crude lipid was formulated as the basal and control, on which other two protein and lipid contents identical experimental diets were formulated by supplementing the basal with either 0.75% Pro(Pro-0.75) or 0.75% Pro and 0.75% Hyp(Pro+Hyp). Four groups of fish in indoor seawater recirculating systems, 35 individuals each, were fed twice a day to apparent satiation for 10 weeks. The results showed that dietary Pro and Hyp supplementation had no significant effect on growth performance and feed utilization of juvenile turbot(P > 0.05). Total Hyp and collagen concentrations in muscle were significantly increased when dietary Pro and Hyp increased(P < 0.05), and fish fed diet Pro+Hyp showed significantly higher free Hyp content in plasma than those fed other diets(P < 0.05). The expression of P4 H α(I) gene in liver and muscle was significantly up regulated in fish fed diet Pro-0.75 in comparison with control(P < 0.05); however the gene was significantly down regulated in fish fed diet Pro+Hyp in muscle in comparison with fish fed diet Pro-0.75(P < 0.05). It can be concluded that supplement of crystal L-Pro and L-Hyp to high plant protein diets did not show positive effects on growth performance of juvenile turbot, but enhanced total collagen concentrations in muscle.展开更多
An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid...An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplastderived cells reached uP to over 60 %, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were regenerated.Chromosome number of all protoplast-regenerated plants examined were normal (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP.展开更多
A total of 16 Friesian cows were used in the study to determine the peripheral concentration of hydroxyproline(HYPRO) and 3 methyl histidine(3 MEHIS).The objectives of the experiment were to find out the relationshi...A total of 16 Friesian cows were used in the study to determine the peripheral concentration of hydroxyproline(HYPRO) and 3 methyl histidine(3 MEHIS).The objectives of the experiment were to find out the relationship between collagen and myosin degradation and uterine involution in the postpartum cow.The results showed tht the mean concentrations of HYPRO and 3 MEHIS were 13.47±2.38 and 18.35±2.77 n mole/mL,respectively,for the cows during the days 60～90 postpartum.And for the cows immediately after calving the concentrations of HYPRO and 3 HEHIS reduced from 34.29 and 28.06 nmole/mL on the day one after calving to the normal lever of 13.23 and 17.97 n mole on the day 50 postpartum,respectively.There was a good correlation between the peripheral concentrations of both HYPRO and 3 MEHIS and the diameters of the involuting uterus(r=0.8128).展开更多
The urinary concentrations of hydroxyproline (HYPRO)and 3 -methyl histidine (3 - MEHIS) were de- termined in 16 Chinese-Holstein cows. The objectives of the experiment were to find out thhe relationship between collag...The urinary concentrations of hydroxyproline (HYPRO)and 3 -methyl histidine (3 - MEHIS) were de- termined in 16 Chinese-Holstein cows. The objectives of the experiment were to find out thhe relationship between collagen and myosin ddegradation and uterine involution in the postpartum cow. The results in the experiment showed that the mean concentrations of HYPRO and 3-MEHIS were 138.32±22.99 and 37.09 ±3.90 nmol·mL-1,respectively,for the cows during the days between 60-90 postpartum,and for the cows immediately after calving the concentrations of HYPRO and 3 -MEHIS reduced from 284.30 and 65.48 nmol ·mL-1 on the day one after calving to the normal level of 109.18 and 33.51 nmol·mL^-1 on the day 50 post- partum,respectively. There was a good correlation between the urinary concentrations of both HYPRO and 3 -MEHIS and the diameters of the involuting uterus (r = 0. 79).展开更多
Vitamin C is a water soluble vitamin found in many natural sources, citric fruits, vegetables, particularly as antioxidants and as a factor to catabolism and metabolism. The aim of this article is to correlate the lev...Vitamin C is a water soluble vitamin found in many natural sources, citric fruits, vegetables, particularly as antioxidants and as a factor to catabolism and metabolism. The aim of this article is to correlate the level of vitamin C and the hydroxyproline level in urine samples among young palestinian children from age 5 - 14 years. Materials and Methods: Urine samples of 34 individuals of both sexes, their ages ranged from 5 - 14 years were collected and analyzed for determination of Urinary Hydroxyproline by using Modified Neuman and Logan Method. The result of this study shows a significant correlation between Vitamin C and Hydroxyproline at level 0.01. The conclusion of this study was that low vitamin C intake was found among young palestinian children from age 5 - 14 years, Vitamin C should be supplemented in the drinks taken by young palestinian children from age 5 - 14 years as well as further research and investigation with large samples required to include all children from middle and southern Palestine.展开更多
A series of poly（lactic acid-4-hydroxyproline-polyethylene glycol） （PLA-Hpr-PEG） copolymers were synthesized by direct melt copolymerization of D,L-lactic acid and 4-hydroxyproline with different feed amount of po...A series of poly（lactic acid-4-hydroxyproline-polyethylene glycol） （PLA-Hpr-PEG） copolymers were synthesized by direct melt copolymerization of D,L-lactic acid and 4-hydroxyproline with different feed amount of polyethylene glycol （PEG） 0.1%, 0.5%, 1% and 5%, respectively. The properties of these copolymers were characterized by using IR spectroscopy, proton nuclear magnetic resonance （1H-NMR） spectroscopy, gel permeation chromatography （GPC）, X-ray diffraction and differential scaning calorimetry （DSC）. PLA-Hpr-PEG are amorphous copolymers. Copolymers showed increasing water uptake capacity with increasing PEG percentage in the feed, which result in an increasing degradable rate in phosphate buffer solution （pH 7.4） at 37℃.展开更多
The content of hydroxyproline of collagen sponge is tested by Woessner Method I.The hydroxyproline standard curve and regression equation are described.The content of hydroxyproline of collagen sponge is tested subseq...The content of hydroxyproline of collagen sponge is tested by Woessner Method I.The hydroxyproline standard curve and regression equation are described.The content of hydroxyproline of collagen sponge is tested subsequently.The result show that the hydroxyproline content is consistent with that of collagen protein,which could be concluded that the purity of collagen sponge,either prepared in our institute or that from abroad is high.展开更多
Hardness is widely regarded as a critical factor influencing the whole texture of fish flesh.The objective of this study was to elucidate the regulatory mechanism underlying muscle hardness in hybrid bream(BBTB,Megalo...Hardness is widely regarded as a critical factor influencing the whole texture of fish flesh.The objective of this study was to elucidate the regulatory mechanism underlying muscle hardness in hybrid bream(BBTB,Megalobrama amblycephala♀×Culter alburnus♂).A comparison of the physiological features of high hardness(HH)and low hardness(LH)muscle revealed that the former had higher contents of collagen I and lower muscle fiber diameter.Transcriptomic data revealed that the myofiber assembly pathway and the HIF-1 signaling pathway were activated in HH muscle.At the metabolic level,the categories of amino acids and lipids were the principal differentially abundant metabolites between the HH and LH muscle.The detection of amino acid profiles further revealed significant differences in amino acid metabolism between the HH and LH muscles,with the HH muscle having higher levels of amino acids than the LH muscle,especially hydroxypro-line(Hyp).Furthermore,through supplementation of Hyp in BBTB myoblasts,the results indicated that 0.8 mmol/L Hyp increased the proliferation,differentiation,migration,and collagen synthesis of myoblasts.Finally,BBTB was treated with Hyp intraperitoneally for 15 days.The results revealed that 0.1 g/kg Hyp significantly increased muscle hardness,myofiber density,myofibrillar protein synthesis,and HIF-1 protein content.The results obtained in this study indicated that Hyp supplementation promoted collagen synthesis and proliferation of myoblast and muscle fibers in the BBTB,which may be induced by activation of the transcription factor HIF1 and contributes to the impacts of Hyp on improvements in muscle hardness in the BBTB.展开更多
This study investigated the effects of hydroxyproline(Hyp)and vitamin C(VC)addition to practical diet on growth and flesh quality of Pacific white shrimp(Litopenaeus vannamei)cultured in low salinity water.The control...This study investigated the effects of hydroxyproline(Hyp)and vitamin C(VC)addition to practical diet on growth and flesh quality of Pacific white shrimp(Litopenaeus vannamei)cultured in low salinity water.The control diet was formulated to contain 200 g/kg fish meal(FM20),and then 14 g/kg coated hydroxyproline(50%)(FM20H),2 g/kg L-ascorbyl-2-phosphate(38%)(FM20C)and their combination(FM20HC)were added to the control diet to form four isoproteic(41.6%)and isolipidic(6.7%)diets.Pacific white shrimp with initial body weight of(1.50±0.05)g were fed the above four diets in low salinity water(salinity 1‰)for 8 weeks.The results showed that the combined supplementation of Hyp and VC(FM20HC)increased the weight gain by 13.4%,and decreased the feed conversion ratio by 0.18(P<0.05),while the individual supplementation(FM20H and FM20C groups)just numerically promoted the growth(P>0.05).In addition,the FM20HC group also showed higher protein and lipid retention than the FM20 group(P<0.05).In flesh quality,the supple-mentation of Hyp(FM20H)increased total collagen and heat-soluble collagen contents,flesh chewiness and free flavor amino acids contents(P<0.05).The supplementation of vC(FM20C)increased the contents of total collagen,heat-insoluble collagen and free flavor amino acids,and decreased flesh lactic acid,malondialdehyde contents and steaming loss(P<0.05).The combined supplementation of Hyp and VC(FM20HC)showed higher total collagen and heat-insoluble collagen contents than the individual supplementation(FM20H and FM20C)(P<0.05),and the myofiber density,flesh hardness and shear force in the FM20HC group were also significantly higher than those in the control(P<0.05).In summary,the combined supplementation of hydroxyproline and vc in practical diet with fishmeal content of 200 g/kg significantly improved the growth performance and flesh quality of L.vannamei cultured in low salinity water.展开更多
Purpose The purpose of this study is to review the urine products of bone breakdown as markers of bone resorption and usefulness of urinary hydroxyproline.Data Related researches published in 1985 -2000 were systemati...Purpose The purpose of this study is to review the urine products of bone breakdown as markers of bone resorption and usefulness of urinary hydroxyproline.Data Related researches published in 1985 -2000 were systematically reviewed.Results Bone markers could be used for early diagnosis of bone metabolic diseases. Biochemical markers of bone resorption that reflect osteoclast activity and/or collagen degradation provide a new and potentially important clinical tool for the assessment and monitoring of bone metabolism. Assessment of bone resorption can be achieved with measurement of urinary hydroxylysine glycosides, urinary excretion of the collagen pyridinium cross-links, urinary excretion of type I collagen telopeptide breakdown products (cross-linked telopeptides) and urinary hydroxyproline.Conclusion Urinary hydroxyproline has been in use as a marker of bone resorption, but it lacks sensitivity and specificity. It is a modified aminoacid that is a metabolic product of collagen breakdown. Hydroxyproline may be released either free or with fragments of the collagen molecule attached during bone resorption, and it is also liberated by the breakdown of complement and nonskeletal collagen.展开更多
This experiment aimed to evaluate the impact of dietary hydroxyproline(Hyp)supplementation on the muscle quality of juvenile Pacific white shrimp(Litopenaeus vannamei)fed a low fishmeal diet.Six formulated diets inclu...This experiment aimed to evaluate the impact of dietary hydroxyproline(Hyp)supplementation on the muscle quality of juvenile Pacific white shrimp(Litopenaeus vannamei)fed a low fishmeal diet.Six formulated diets included one high fishmeal(HF;25%fishmeal content)and five low fishmeal diets(10%fishmeal content)with 0%,0.2%,0.4%,0.6%and 0.8%Hyp(LFO,LF2,LF4,LF6 and LF8,respectively).Each diet was assigned to four replicates,and 40 shrimp(0.32±0.00 g)per replicate were fed four times a day for 8 weeks.Dietary Hyp supplementation had little effects on growth performance,but increased the contents of Hyp,prolyl 4-hydroxylases(P4Hs),and collagen.The meat yield,springiness,hardness,chewiness,and cohesiveness of muscle were the highest in the LF4 group among the low fishmeal groups(P<0.05).Cooking loss and freezing loss of muscle were the lowest in the LF4 group(P<0.05).Dietary supplementation with 0.4%Hyp increased the myofiber density and decreased the myofiber diameter of muscle(P<0.05).Supplementation of Hyp in the diet up-regulated the mRNA expression of smyhc5,smyhc15,col1a1,col1a2,igf-1f,tgf-β and tor and down-regulated the mRNA expression of smy hc1,smyhc2,smyhc6a(P<0.05).Supplementation of Hyp in the diet up-regulated the protein expression of P-4E-BP1,P-AKT,AKT and P-AKT/AKT(P<0.05).These results suggested that the addition of 0.4%Hyp to low fishmeal diets improved the muscle quality of L.vannamei.展开更多
Oxidative stress has been confirmed in relation to intestinal mucosa damage and multiple bowel diseases.Hydroxyproline (Hyp) is an imino acid abundant in sow's milk. Compelling evidence has beengathered showing th...Oxidative stress has been confirmed in relation to intestinal mucosa damage and multiple bowel diseases.Hydroxyproline (Hyp) is an imino acid abundant in sow's milk. Compelling evidence has beengathered showing the potential antioxidative properties of Hyp. However, the role and mechanism ofHyp in porcine intestinal epithelial cells in response to oxidative stress remains unknown. In this study,small intestinal epithelial cell lines of piglets (IPEC-1) were used to evaluate the protective effects of Hypon 4-hydroxy-2-nonenal (4-HNE)-induced oxidative DNA damage and apoptosis. IPEC-1 pretreated with0.5 to 5 mmol/L Hyp were exposed to 4-HNE (40 mmol/L) in the presence or absence of Hyp. Thereafter,the cells were subjected to apoptosis detection by Hoechst staining, flow cytometry, and Western blot orDNA damage analysis by comet assay, immunofluorescence, and reverse-transcription quantitative PCR(RT-qPCR). Cell apoptosis and the upregulation of cleaved-caspase-3 induced by 4-HNE (40 mmol/L) wereinhibited by 5 mmol/L of Hyp. In addition, 5 mmol/L Hyp attenuated 4-HNE-induced reactive oxygenspecies (ROS) accumulation, glutathione (GSH) deprivation and DNA damage. The elevation in transcriptionof GADD45a (growth arrest and DNA-damage-inducible protein 45 alpha) and GADD45b(growth arrest and DNA-damage-inducible protein 45 beta), as well as the phosphorylation of H2AX(H2A histone family, member X), p38 MAPK (mitogen-activated protein kinase), and JNK (c-Jun N-terminalkinase) in cells treated with 4-HNE were alleviated by 5 mmol/L Hyp. Furthermore, Hyp supplementationincreased the protein abundance of Krüppel like factor 4 (KLF4) in cells exposed to 4-HNE.Suppression of KLF4 expression by kenpaulone impeded the resistance of Hyp-treated cells to DNAdamage and apoptosis induced by 4-HNE. Collectively, our results indicated that Hyp serves to protectagainst 4-HNE-induced apoptosis and DNA damage in IPEC-1 cells, which is partially pertinent with theenhanced expression of KLF4. Our data provides an updated explanation for the nutritional values ofHyp-containing animal products.展开更多
文摘Fifteen cases of fresh fractures have been studied, including 14 cases of tubu-lar bone fractures with age from 28 to 60 years and one case of vertebral fracture ofdorsolumbar region, 16 years of age. There were 13 male and 2 female patients. Theyhad no endocrine disturbances or other bone diseases. The ratio ofhydroxyproline/creatinine (HP/Cr) in these cases increased gradually within 20-30 daysafter fracture and then reached the peak value which was about 3-4 times of normal, thepeak value maintained for a long time. If the trauma was mild or the patient was young,the peak began to decline on the 60th day after fracture. While in those severe cases withprolonged confinement to bed, the peak only began to come down about 110th dayafter fracture. The ratio of HP/Cr decreased rapidly from the peak at first, then pursueda slow course. Generally, the ratio of HP/Cr returned to normal on the 240th to 310thday. Bone union began at a time when peak value started to decline, and with completionof the remodelling process, the HP/Cr value returned to normal.
基金financially supported by the China Agriculture Research System (CARS-50-G08)Agricultural Scientific and Technological Achievements into Capital (2010GB23600673)the National Natural Science Foundation of China (Grant Nos. 31072222 and 30901 108)
文摘This study was conducted to investigate the effect of dietary proline(Pro), and Pro and hydroxyproline(Hyp) in combination on the growth performance, total Hyp and collagen concentrations of tissues, and prolyl 4-hydroxylase α(I)(P4H α(I)) gene expression in juvenile turbot feeding high plant protein diets. A diet containing 50% crude protein and 12% crude lipid was formulated as the basal and control, on which other two protein and lipid contents identical experimental diets were formulated by supplementing the basal with either 0.75% Pro(Pro-0.75) or 0.75% Pro and 0.75% Hyp(Pro+Hyp). Four groups of fish in indoor seawater recirculating systems, 35 individuals each, were fed twice a day to apparent satiation for 10 weeks. The results showed that dietary Pro and Hyp supplementation had no significant effect on growth performance and feed utilization of juvenile turbot(P > 0.05). Total Hyp and collagen concentrations in muscle were significantly increased when dietary Pro and Hyp increased(P < 0.05), and fish fed diet Pro+Hyp showed significantly higher free Hyp content in plasma than those fed other diets(P < 0.05). The expression of P4 H α(I) gene in liver and muscle was significantly up regulated in fish fed diet Pro-0.75 in comparison with control(P < 0.05); however the gene was significantly down regulated in fish fed diet Pro+Hyp in muscle in comparison with fish fed diet Pro-0.75(P < 0.05). It can be concluded that supplement of crystal L-Pro and L-Hyp to high plant protein diets did not show positive effects on growth performance of juvenile turbot, but enhanced total collagen concentrations in muscle.
文摘An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplastderived cells reached uP to over 60 %, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were regenerated.Chromosome number of all protoplast-regenerated plants examined were normal (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP.
基金The project is supported financially by Heilongjiang and Chinese ntional and Scientific fund
文摘A total of 16 Friesian cows were used in the study to determine the peripheral concentration of hydroxyproline(HYPRO) and 3 methyl histidine(3 MEHIS).The objectives of the experiment were to find out the relationship between collagen and myosin degradation and uterine involution in the postpartum cow.The results showed tht the mean concentrations of HYPRO and 3 MEHIS were 13.47±2.38 and 18.35±2.77 n mole/mL,respectively,for the cows during the days 60～90 postpartum.And for the cows immediately after calving the concentrations of HYPRO and 3 HEHIS reduced from 34.29 and 28.06 nmole/mL on the day one after calving to the normal lever of 13.23 and 17.97 n mole on the day 50 postpartum,respectively.There was a good correlation between the peripheral concentrations of both HYPRO and 3 MEHIS and the diameters of the involuting uterus(r=0.8128).
基金supported financially by Heilongjiang(C- 9702)and Chinese National (39870572)Natural and Scicntificfunds.
文摘The urinary concentrations of hydroxyproline (HYPRO)and 3 -methyl histidine (3 - MEHIS) were de- termined in 16 Chinese-Holstein cows. The objectives of the experiment were to find out thhe relationship between collagen and myosin ddegradation and uterine involution in the postpartum cow. The results in the experiment showed that the mean concentrations of HYPRO and 3-MEHIS were 138.32±22.99 and 37.09 ±3.90 nmol·mL-1,respectively,for the cows during the days between 60-90 postpartum,and for the cows immediately after calving the concentrations of HYPRO and 3 -MEHIS reduced from 284.30 and 65.48 nmol ·mL-1 on the day one after calving to the normal level of 109.18 and 33.51 nmol·mL^-1 on the day 50 post- partum,respectively. There was a good correlation between the urinary concentrations of both HYPRO and 3 -MEHIS and the diameters of the involuting uterus (r = 0. 79).
文摘Vitamin C is a water soluble vitamin found in many natural sources, citric fruits, vegetables, particularly as antioxidants and as a factor to catabolism and metabolism. The aim of this article is to correlate the level of vitamin C and the hydroxyproline level in urine samples among young palestinian children from age 5 - 14 years. Materials and Methods: Urine samples of 34 individuals of both sexes, their ages ranged from 5 - 14 years were collected and analyzed for determination of Urinary Hydroxyproline by using Modified Neuman and Logan Method. The result of this study shows a significant correlation between Vitamin C and Hydroxyproline at level 0.01. The conclusion of this study was that low vitamin C intake was found among young palestinian children from age 5 - 14 years, Vitamin C should be supplemented in the drinks taken by young palestinian children from age 5 - 14 years as well as further research and investigation with large samples required to include all children from middle and southern Palestine.
文摘A series of poly（lactic acid-4-hydroxyproline-polyethylene glycol） （PLA-Hpr-PEG） copolymers were synthesized by direct melt copolymerization of D,L-lactic acid and 4-hydroxyproline with different feed amount of polyethylene glycol （PEG） 0.1%, 0.5%, 1% and 5%, respectively. The properties of these copolymers were characterized by using IR spectroscopy, proton nuclear magnetic resonance （1H-NMR） spectroscopy, gel permeation chromatography （GPC）, X-ray diffraction and differential scaning calorimetry （DSC）. PLA-Hpr-PEG are amorphous copolymers. Copolymers showed increasing water uptake capacity with increasing PEG percentage in the feed, which result in an increasing degradable rate in phosphate buffer solution （pH 7.4） at 37℃.
文摘The content of hydroxyproline of collagen sponge is tested by Woessner Method I.The hydroxyproline standard curve and regression equation are described.The content of hydroxyproline of collagen sponge is tested subsequently.The result show that the hydroxyproline content is consistent with that of collagen protein,which could be concluded that the purity of collagen sponge,either prepared in our institute or that from abroad is high.
基金supported by the National Key Research and Development Plan Program of China(2022YFD2400600/2022YFD2400601/2022YFD2400603)the Science and Technology Innovation Program of Hunan Province(Grant No.2023RC1072)+1 种基金Scientific Research Fund of Hunan Provincial Education Department(Grant No.24B0799)the earmarked fund for HARS(HARS-07).
文摘Hardness is widely regarded as a critical factor influencing the whole texture of fish flesh.The objective of this study was to elucidate the regulatory mechanism underlying muscle hardness in hybrid bream(BBTB,Megalobrama amblycephala♀×Culter alburnus♂).A comparison of the physiological features of high hardness(HH)and low hardness(LH)muscle revealed that the former had higher contents of collagen I and lower muscle fiber diameter.Transcriptomic data revealed that the myofiber assembly pathway and the HIF-1 signaling pathway were activated in HH muscle.At the metabolic level,the categories of amino acids and lipids were the principal differentially abundant metabolites between the HH and LH muscle.The detection of amino acid profiles further revealed significant differences in amino acid metabolism between the HH and LH muscles,with the HH muscle having higher levels of amino acids than the LH muscle,especially hydroxypro-line(Hyp).Furthermore,through supplementation of Hyp in BBTB myoblasts,the results indicated that 0.8 mmol/L Hyp increased the proliferation,differentiation,migration,and collagen synthesis of myoblasts.Finally,BBTB was treated with Hyp intraperitoneally for 15 days.The results revealed that 0.1 g/kg Hyp significantly increased muscle hardness,myofiber density,myofibrillar protein synthesis,and HIF-1 protein content.The results obtained in this study indicated that Hyp supplementation promoted collagen synthesis and proliferation of myoblast and muscle fibers in the BBTB,which may be induced by activation of the transcription factor HIF1 and contributes to the impacts of Hyp on improvements in muscle hardness in the BBTB.
基金supported by the National Key R&D Program of China(2019YFD0900200).
文摘This study investigated the effects of hydroxyproline(Hyp)and vitamin C(VC)addition to practical diet on growth and flesh quality of Pacific white shrimp(Litopenaeus vannamei)cultured in low salinity water.The control diet was formulated to contain 200 g/kg fish meal(FM20),and then 14 g/kg coated hydroxyproline(50%)(FM20H),2 g/kg L-ascorbyl-2-phosphate(38%)(FM20C)and their combination(FM20HC)were added to the control diet to form four isoproteic(41.6%)and isolipidic(6.7%)diets.Pacific white shrimp with initial body weight of(1.50±0.05)g were fed the above four diets in low salinity water(salinity 1‰)for 8 weeks.The results showed that the combined supplementation of Hyp and VC(FM20HC)increased the weight gain by 13.4%,and decreased the feed conversion ratio by 0.18(P<0.05),while the individual supplementation(FM20H and FM20C groups)just numerically promoted the growth(P>0.05).In addition,the FM20HC group also showed higher protein and lipid retention than the FM20 group(P<0.05).In flesh quality,the supple-mentation of Hyp(FM20H)increased total collagen and heat-soluble collagen contents,flesh chewiness and free flavor amino acids contents(P<0.05).The supplementation of vC(FM20C)increased the contents of total collagen,heat-insoluble collagen and free flavor amino acids,and decreased flesh lactic acid,malondialdehyde contents and steaming loss(P<0.05).The combined supplementation of Hyp and VC(FM20HC)showed higher total collagen and heat-insoluble collagen contents than the individual supplementation(FM20H and FM20C)(P<0.05),and the myofiber density,flesh hardness and shear force in the FM20HC group were also significantly higher than those in the control(P<0.05).In summary,the combined supplementation of hydroxyproline and vc in practical diet with fishmeal content of 200 g/kg significantly improved the growth performance and flesh quality of L.vannamei cultured in low salinity water.
文摘Purpose The purpose of this study is to review the urine products of bone breakdown as markers of bone resorption and usefulness of urinary hydroxyproline.Data Related researches published in 1985 -2000 were systematically reviewed.Results Bone markers could be used for early diagnosis of bone metabolic diseases. Biochemical markers of bone resorption that reflect osteoclast activity and/or collagen degradation provide a new and potentially important clinical tool for the assessment and monitoring of bone metabolism. Assessment of bone resorption can be achieved with measurement of urinary hydroxylysine glycosides, urinary excretion of the collagen pyridinium cross-links, urinary excretion of type I collagen telopeptide breakdown products (cross-linked telopeptides) and urinary hydroxyproline.Conclusion Urinary hydroxyproline has been in use as a marker of bone resorption, but it lacks sensitivity and specificity. It is a modified aminoacid that is a metabolic product of collagen breakdown. Hydroxyproline may be released either free or with fragments of the collagen molecule attached during bone resorption, and it is also liberated by the breakdown of complement and nonskeletal collagen.
基金supported by the fund of the National Natural Science Foundation of China(32373141)the Guangdong Basic and Applied Basic Research Foundation(2023A1515030007)ModernSeed Industry Park for Whiteleg Shrimp of Guangdong Province,program for scientific research start-up funds of Guangdong Ocean University,Science and Technology Bureau of Zhanjiang(2020A05003,2022A 01002).
文摘This experiment aimed to evaluate the impact of dietary hydroxyproline(Hyp)supplementation on the muscle quality of juvenile Pacific white shrimp(Litopenaeus vannamei)fed a low fishmeal diet.Six formulated diets included one high fishmeal(HF;25%fishmeal content)and five low fishmeal diets(10%fishmeal content)with 0%,0.2%,0.4%,0.6%and 0.8%Hyp(LFO,LF2,LF4,LF6 and LF8,respectively).Each diet was assigned to four replicates,and 40 shrimp(0.32±0.00 g)per replicate were fed four times a day for 8 weeks.Dietary Hyp supplementation had little effects on growth performance,but increased the contents of Hyp,prolyl 4-hydroxylases(P4Hs),and collagen.The meat yield,springiness,hardness,chewiness,and cohesiveness of muscle were the highest in the LF4 group among the low fishmeal groups(P<0.05).Cooking loss and freezing loss of muscle were the lowest in the LF4 group(P<0.05).Dietary supplementation with 0.4%Hyp increased the myofiber density and decreased the myofiber diameter of muscle(P<0.05).Supplementation of Hyp in the diet up-regulated the mRNA expression of smyhc5,smyhc15,col1a1,col1a2,igf-1f,tgf-β and tor and down-regulated the mRNA expression of smy hc1,smyhc2,smyhc6a(P<0.05).Supplementation of Hyp in the diet up-regulated the protein expression of P-4E-BP1,P-AKT,AKT and P-AKT/AKT(P<0.05).These results suggested that the addition of 0.4%Hyp to low fishmeal diets improved the muscle quality of L.vannamei.
基金the National Natural Science Foundation of China(No.31625025,31301979)the Zhengzhou 1125 Talent Program,and the Jinxinnong Animal Science Development Foundation.
文摘Oxidative stress has been confirmed in relation to intestinal mucosa damage and multiple bowel diseases.Hydroxyproline (Hyp) is an imino acid abundant in sow's milk. Compelling evidence has beengathered showing the potential antioxidative properties of Hyp. However, the role and mechanism ofHyp in porcine intestinal epithelial cells in response to oxidative stress remains unknown. In this study,small intestinal epithelial cell lines of piglets (IPEC-1) were used to evaluate the protective effects of Hypon 4-hydroxy-2-nonenal (4-HNE)-induced oxidative DNA damage and apoptosis. IPEC-1 pretreated with0.5 to 5 mmol/L Hyp were exposed to 4-HNE (40 mmol/L) in the presence or absence of Hyp. Thereafter,the cells were subjected to apoptosis detection by Hoechst staining, flow cytometry, and Western blot orDNA damage analysis by comet assay, immunofluorescence, and reverse-transcription quantitative PCR(RT-qPCR). Cell apoptosis and the upregulation of cleaved-caspase-3 induced by 4-HNE (40 mmol/L) wereinhibited by 5 mmol/L of Hyp. In addition, 5 mmol/L Hyp attenuated 4-HNE-induced reactive oxygenspecies (ROS) accumulation, glutathione (GSH) deprivation and DNA damage. The elevation in transcriptionof GADD45a (growth arrest and DNA-damage-inducible protein 45 alpha) and GADD45b(growth arrest and DNA-damage-inducible protein 45 beta), as well as the phosphorylation of H2AX(H2A histone family, member X), p38 MAPK (mitogen-activated protein kinase), and JNK (c-Jun N-terminalkinase) in cells treated with 4-HNE were alleviated by 5 mmol/L Hyp. Furthermore, Hyp supplementationincreased the protein abundance of Krüppel like factor 4 (KLF4) in cells exposed to 4-HNE.Suppression of KLF4 expression by kenpaulone impeded the resistance of Hyp-treated cells to DNAdamage and apoptosis induced by 4-HNE. Collectively, our results indicated that Hyp serves to protectagainst 4-HNE-induced apoptosis and DNA damage in IPEC-1 cells, which is partially pertinent with theenhanced expression of KLF4. Our data provides an updated explanation for the nutritional values ofHyp-containing animal products.
