Correction to:Rare Met.https://doi.org/10.1007/s12598-021-01815-z In the original publication,Fig.5 was published with few mistakes.The correct version of Fig.5 is given in this correction.
为深入研究绣球属植物花粉形态的分类学价值和系统学意义,厘清绣球属与近缘属之间的系统发育关系,该文利用扫描电子显微镜(SEM,scanning electron microscope)对国产绣球属及其近缘属41种绣球花科(Hydrangeaceae)植物的花粉形态以及表...为深入研究绣球属植物花粉形态的分类学价值和系统学意义,厘清绣球属与近缘属之间的系统发育关系,该文利用扫描电子显微镜(SEM,scanning electron microscope)对国产绣球属及其近缘属41种绣球花科(Hydrangeaceae)植物的花粉形态以及表面纹饰进行了观察。结果表明:绣球属及其近缘属的花粉为三孔沟;形状多数为长圆体形或近球体形;赤道面观为椭圆形或圆形;极面观多为圆形,少数为三角形或圆三角形。花粉外壁纹饰可分为网状和孔穴状。网眼内的三级纹饰可分为光滑和具颗粒状突起。根据花粉形状和外壁纹饰类型将上述物种划分为4个组,即花粉的形状为长圆体形,表面纹饰为孔穴纹饰;花粉的形状为长圆体形,表面纹饰为网状纹饰;花粉的形状为近球体形,表面纹饰为孔穴纹饰;花粉的形状为近球体形,表面纹饰为网状纹饰。以上可进一步细分为8个类型。上述表明花粉形态证据可为绣球属及其近缘属的属下分类和种间界定提供重要佐证;但结合前人的系统发育重建分析该属植物花粉形态的系统学意义相对有限,如花粉形态证据对于该属及其近缘种属系统发育树上大支的界定难以提供有力的证据。展开更多
Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-sta...Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-stage and strengthen their resistance to adverse conditions.In this study,nitrogen requirements for producing Hydrangea macrophylla‘Hanatemari’that robust seedlings,nutrient dynamics,biomass and growth,was examined utilizing exponential fertilization.The potted seedlings were fertilized with urea under exponential regime at rates of 0.5,1.5 and 2.0 g nitrogen/plant(EF1,EF2,and EF3),respectively.In addition,an unfertilized group treated with equal volume of deionized water was used as control.The results showed that seedlings under 1.5 g N/plant(EF2)had the highest plant growth index and total biomass.The nutrient concentrations of different organs varied in different fertilization treatments.Based on the results of current study,it is concluded that 1.5 g N/plant(EF2)is suitable exponential fertilization treatment for the culture of hydrangea seedlings.Our treatments results showed that 2.0 g N/plant is not suitable for seedling culturing,because of serious nutrient toxicity.These findings will help to improve seedling quality and strengthen the production of H.macrophylla for plantation.展开更多
A new kaempferol glycoside,named kaempferol 3-O-[6″-O-β-D-glucopyranosyl-6′′′-O-α-L-rhamnopy ranosyl-2′′′′-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside,was isolated from Hydrangea macrophylla(Thunb.) Seringe...A new kaempferol glycoside,named kaempferol 3-O-[6″-O-β-D-glucopyranosyl-6′′′-O-α-L-rhamnopy ranosyl-2′′′′-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside,was isolated from Hydrangea macrophylla(Thunb.) Seringe.Its structure was establishedby spectroscopic techniques including MS,IR,UV,and 2D NMR.展开更多
Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we establish...Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we established an efficient regeneration system by using stem segments as explants for the first time.In our study,the plant growth regulators(PGRs)were evaluated at the different regeneration processes,including axillary shoots regeneration and root induction.We found that the optimal concentration for axillary buds’induction was 2.0 mgL^(-1)6-BA and 0.5 mgL^(-1)1 IAA,its highest induction rate was 70%.Moreover,the highest axillary shoots proliferation coefficient was 10.7 on the Murashige and Skoog(MS)medium with 2.0 mgL^(-1)6-benzyladenine(BA),0.2 mgL^(-1)indole-3-butyric acid(IBA),and 1.0 mgL^(-1)gibberellin A3(GA3).The highest frequency of root induction was 80.0±0.06%by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL^(-1)IBA.In summary,our study will provide an effective technology for large-scale propagation and important pathway for promoting the popularization and application of Hydrangea bretschneideri Dipp.展开更多
Objective:To examine the inhibitory effect of Hydrangea serrata extract against hepatocellular carcinoma HepG2 cells and its underlying mechanisms.Methods:The effects of Hydrangea serrata extract on growth inhibition ...Objective:To examine the inhibitory effect of Hydrangea serrata extract against hepatocellular carcinoma HepG2 cells and its underlying mechanisms.Methods:The effects of Hydrangea serrata extract on growth inhibition of tumor cells and spheroids were assessed using MTT and 3D culture assays.Quantitative real-time PCR and Western blot analyses were employed to investigate the changes in mRNA and protein expression levels of molecules related to cell cycle and apoptosis.Results:Hydrangea serrata extract effectively inhibited the growth of both tumor cells and spheroids.The extract also significantly upregulated p27 mRNA expression and downregulated CDK2 mRNA expression,leading to cell cycle arrest.Moreover,increased BAX/Bcl-2 ratio as well as caspase-9 and-3 were observed after treatment with Hydrangea serrata extract,indicating the induction of tumor cell apoptosis.Conclusions:Hydrangea serrata extract has the potential to alleviate tumors by effectively modulating cell-cycle-related gene expressions and inducing apoptosis,thereby inhibiting tumor growth.展开更多
Hydrangea macrophylla is a popular ornamental shrub with a lot of economic and aesthetic value.It is known for its different flower shapes(lacecap and mophead)and the way its flowers change color depending on the pH o...Hydrangea macrophylla is a popular ornamental shrub with a lot of economic and aesthetic value.It is known for its different flower shapes(lacecap and mophead)and the way its flowers change color depending on the pH of the soil.Even though it is important for gardening,we still don’t know much about the molecular processes that lead to flower growth.The purpose of this study was to find and study SNP-related genes and transcription factors that are connected to the growth of H.macrophylla flowers.Genome-wide SNP analysis identified 11 SNPs associated with MYB transcription factors and 10 SNPs linked to a MADS-box SEP1 gene,highlighting their potential role in inflorescence-type regulation.These SNPs provide genomic resources for functional validation and markerassisted breeding in Hydrangea macrophylla.We found the MYB and MADS-box gene families,which are important for pigmentation and flower organ identity,through an analysis of the transcriptome and gene expression.The MYB family has 731R-MYBs,105 R2R3-MYBs,and 43R-MYBs.TheMADS-box family had 42 Type I(M-type)members and 36 Type II(MIKC-type)members.Motif and phylogenetic analysis showed that certain domains were preserved.For example,R2R3-MYBs and MIKC-type MADS genes are grouped with Arabidopsis orthologs,which suggests that their functions are also preserved.There was a clear link between the greatest expression ofMADS-box genes and the distinct phases of floral bud differentiation.Some MYB genes,on the other hand,showed alternative expression patterns that may help petals or sepals develop.qRT-PCR validation of representative MYB and MADS-box genes corroborated the transcriptome-based expression profiles,supporting their role in flower development and inflorescence-type regulation.展开更多
Objective:To determine the effects of Hydrangeae Dulcis Folium(EHDF)on physical stress,changes in the whole-body cortisol level and behaviour in zebrafish(Danio rerio).Methods:One hundred and seventy-four fish were ra...Objective:To determine the effects of Hydrangeae Dulcis Folium(EHDF)on physical stress,changes in the whole-body cortisol level and behaviour in zebrafish(Danio rerio).Methods:One hundred and seventy-four fish were randomly divided into 4[adrenocorticotropin hormone(ACTH)challenge test:4 fish per group]or 6 groups(behavioural test:10–12 fish per group,whole-body cortisol:4 fish per group).Net handling stress(NHS)was used to induce physical stress.Fish were treated with vehicle or EHDF(5–20 mg/L)for 6 min before they were exposed to stress.And then,fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests,including novel tank test and open field test,and were evaluated to observe anxiety-like behaviours and locomotion.In addition,to elucidate the mode of action of the anti-stress effects of EHDF,ACTH(0.2 IU/g,i.p.)challenge test was performed.Results:The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5–20 mg/L(P<0.05).Moreover,compared with the unstressed group,which was not treated with NHS,the whole-body cortisol level was significantly increased by treatment with NHS(P<0.05).Compared with the NHS-treated stressed control group,pre-treatment with EHDF at concentrations of 5–20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level(P<0.05).In addition,ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion(P<0.05).Conclusion:EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.展开更多
文摘Correction to:Rare Met.https://doi.org/10.1007/s12598-021-01815-z In the original publication,Fig.5 was published with few mistakes.The correct version of Fig.5 is given in this correction.
文摘为深入研究绣球属植物花粉形态的分类学价值和系统学意义,厘清绣球属与近缘属之间的系统发育关系,该文利用扫描电子显微镜(SEM,scanning electron microscope)对国产绣球属及其近缘属41种绣球花科(Hydrangeaceae)植物的花粉形态以及表面纹饰进行了观察。结果表明:绣球属及其近缘属的花粉为三孔沟;形状多数为长圆体形或近球体形;赤道面观为椭圆形或圆形;极面观多为圆形,少数为三角形或圆三角形。花粉外壁纹饰可分为网状和孔穴状。网眼内的三级纹饰可分为光滑和具颗粒状突起。根据花粉形状和外壁纹饰类型将上述物种划分为4个组,即花粉的形状为长圆体形,表面纹饰为孔穴纹饰;花粉的形状为长圆体形,表面纹饰为网状纹饰;花粉的形状为近球体形,表面纹饰为孔穴纹饰;花粉的形状为近球体形,表面纹饰为网状纹饰。以上可进一步细分为8个类型。上述表明花粉形态证据可为绣球属及其近缘属的属下分类和种间界定提供重要佐证;但结合前人的系统发育重建分析该属植物花粉形态的系统学意义相对有限,如花粉形态证据对于该属及其近缘种属系统发育树上大支的界定难以提供有力的证据。
基金This research is supported by Science and Technology Research Project of Shanghai Greening and City Appearance Administration in 2019(G192402).
文摘Slow growth rate restricts the development and growth of seedlings due to nutrients deficiency or nutrient imbalance.Exponential fertilization can enhance the internal nutrient reserves in seedlings at the nursery-stage and strengthen their resistance to adverse conditions.In this study,nitrogen requirements for producing Hydrangea macrophylla‘Hanatemari’that robust seedlings,nutrient dynamics,biomass and growth,was examined utilizing exponential fertilization.The potted seedlings were fertilized with urea under exponential regime at rates of 0.5,1.5 and 2.0 g nitrogen/plant(EF1,EF2,and EF3),respectively.In addition,an unfertilized group treated with equal volume of deionized water was used as control.The results showed that seedlings under 1.5 g N/plant(EF2)had the highest plant growth index and total biomass.The nutrient concentrations of different organs varied in different fertilization treatments.Based on the results of current study,it is concluded that 1.5 g N/plant(EF2)is suitable exponential fertilization treatment for the culture of hydrangea seedlings.Our treatments results showed that 2.0 g N/plant is not suitable for seedling culturing,because of serious nutrient toxicity.These findings will help to improve seedling quality and strengthen the production of H.macrophylla for plantation.
基金This work is supported by the Grassland Talent Project:The Innovation Team of New Varieties Breeding at the Economic and Ecological Shrub,and the Evaluation of the Economic and Ecological Shrub Resources and New Variety Breeding in Inner Mongolia(No.201702077)。
文摘Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration.Genetic engineering technology has been successfully used in many plant species,but it is limited in Hydrangea.Here we established an efficient regeneration system by using stem segments as explants for the first time.In our study,the plant growth regulators(PGRs)were evaluated at the different regeneration processes,including axillary shoots regeneration and root induction.We found that the optimal concentration for axillary buds’induction was 2.0 mgL^(-1)6-BA and 0.5 mgL^(-1)1 IAA,its highest induction rate was 70%.Moreover,the highest axillary shoots proliferation coefficient was 10.7 on the Murashige and Skoog(MS)medium with 2.0 mgL^(-1)6-benzyladenine(BA),0.2 mgL^(-1)indole-3-butyric acid(IBA),and 1.0 mgL^(-1)gibberellin A3(GA3).The highest frequency of root induction was 80.0±0.06%by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL^(-1)IBA.In summary,our study will provide an effective technology for large-scale propagation and important pathway for promoting the popularization and application of Hydrangea bretschneideri Dipp.
基金funded by the GRRC Program of Gyeonggi province[GRRC-KyungHee2023(B01)],Republic of Korea.
文摘Objective:To examine the inhibitory effect of Hydrangea serrata extract against hepatocellular carcinoma HepG2 cells and its underlying mechanisms.Methods:The effects of Hydrangea serrata extract on growth inhibition of tumor cells and spheroids were assessed using MTT and 3D culture assays.Quantitative real-time PCR and Western blot analyses were employed to investigate the changes in mRNA and protein expression levels of molecules related to cell cycle and apoptosis.Results:Hydrangea serrata extract effectively inhibited the growth of both tumor cells and spheroids.The extract also significantly upregulated p27 mRNA expression and downregulated CDK2 mRNA expression,leading to cell cycle arrest.Moreover,increased BAX/Bcl-2 ratio as well as caspase-9 and-3 were observed after treatment with Hydrangea serrata extract,indicating the induction of tumor cell apoptosis.Conclusions:Hydrangea serrata extract has the potential to alleviate tumors by effectively modulating cell-cycle-related gene expressions and inducing apoptosis,thereby inhibiting tumor growth.
基金funded by Science and Technology Research Project of Shanghai Greening and City Appearance Administration in 2023(G232406).
文摘Hydrangea macrophylla is a popular ornamental shrub with a lot of economic and aesthetic value.It is known for its different flower shapes(lacecap and mophead)and the way its flowers change color depending on the pH of the soil.Even though it is important for gardening,we still don’t know much about the molecular processes that lead to flower growth.The purpose of this study was to find and study SNP-related genes and transcription factors that are connected to the growth of H.macrophylla flowers.Genome-wide SNP analysis identified 11 SNPs associated with MYB transcription factors and 10 SNPs linked to a MADS-box SEP1 gene,highlighting their potential role in inflorescence-type regulation.These SNPs provide genomic resources for functional validation and markerassisted breeding in Hydrangea macrophylla.We found the MYB and MADS-box gene families,which are important for pigmentation and flower organ identity,through an analysis of the transcriptome and gene expression.The MYB family has 731R-MYBs,105 R2R3-MYBs,and 43R-MYBs.TheMADS-box family had 42 Type I(M-type)members and 36 Type II(MIKC-type)members.Motif and phylogenetic analysis showed that certain domains were preserved.For example,R2R3-MYBs and MIKC-type MADS genes are grouped with Arabidopsis orthologs,which suggests that their functions are also preserved.There was a clear link between the greatest expression ofMADS-box genes and the distinct phases of floral bud differentiation.Some MYB genes,on the other hand,showed alternative expression patterns that may help petals or sepals develop.qRT-PCR validation of representative MYB and MADS-box genes corroborated the transcriptome-based expression profiles,supporting their role in flower development and inflorescence-type regulation.
基金Supported by the Ministry of Trade,Industry&Energy(MOTIE)Korea Institute for Advancement of Technology(KIAT)through the Encouragement Program for the Industries of Economic Cooperation Region
文摘Objective:To determine the effects of Hydrangeae Dulcis Folium(EHDF)on physical stress,changes in the whole-body cortisol level and behaviour in zebrafish(Danio rerio).Methods:One hundred and seventy-four fish were randomly divided into 4[adrenocorticotropin hormone(ACTH)challenge test:4 fish per group]or 6 groups(behavioural test:10–12 fish per group,whole-body cortisol:4 fish per group).Net handling stress(NHS)was used to induce physical stress.Fish were treated with vehicle or EHDF(5–20 mg/L)for 6 min before they were exposed to stress.And then,fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests,including novel tank test and open field test,and were evaluated to observe anxiety-like behaviours and locomotion.In addition,to elucidate the mode of action of the anti-stress effects of EHDF,ACTH(0.2 IU/g,i.p.)challenge test was performed.Results:The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5–20 mg/L(P<0.05).Moreover,compared with the unstressed group,which was not treated with NHS,the whole-body cortisol level was significantly increased by treatment with NHS(P<0.05).Compared with the NHS-treated stressed control group,pre-treatment with EHDF at concentrations of 5–20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level(P<0.05).In addition,ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion(P<0.05).Conclusion:EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.
