Stem cells play a crucial role in maintaining tissue regenerative capacity and homeostasis.However,mechanisms associated with stem cell senescence require further investigation.In this study,we conducted a proteomic a...Stem cells play a crucial role in maintaining tissue regenerative capacity and homeostasis.However,mechanisms associated with stem cell senescence require further investigation.In this study,we conducted a proteomic analysis of human dental pulp stem cells(HDPSCs)obtained from individuals of various ages.Our findings showed that the expression of NUP62 was decreased in aged HDPSCs.We discovered that NUP62 alleviated senescence-associated phenotypes and enhanced differentiation potential both in vitro and in vivo.Conversely,the knocking down of NUP62 expression aggravated the senescence-associated phenotypes and impaired the proliferation and migration capacity of HDPSCs.Through RNA-sequence and decoding the epigenomic landscapes remodeled induced by NUP62 overexpression,we found that NUP62 helps alleviate senescence in HDPSCs by enhancing the nuclear transport of the transcription factor E2F1.This,in turn,stimulates the transcription of the epigenetic enzyme NSD2.Finally,the overexpression of NUP62 influences the H3K36me2 and H3K36me3 modifications of anti-aging genes(HMGA1,HMGA2,and SIRT6).Our results demonstrated that NUP62 regulates the fate of HDPSCs via NSD2-dependent epigenetic reprogramming.展开更多
Pulpotomy,which belongs to vital pulp therapy,has become a strategy for managing pulpitis in recent decades.This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing l...Pulpotomy,which belongs to vital pulp therapy,has become a strategy for managing pulpitis in recent decades.This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing long-term patient-centered outcomes.Pulpotomy is categorized into partial pulpotomy(PP),the removal of a partial segment of the coronal pulp tissue,and full pulpotomy(FP),the removal of whole coronal pulp,which is followed by applying the biomaterials onto the remaining pulp tissue and ultimately restoring the tooth.Procedural decisions for the amount of pulp tissue removal or retention depend on the diagnostic of pulp vitality,the overall treatment plan,the patient’s general health status,and pulp inflammation reassessment during operation.This statement represents the consensus of an expert committee convened by the Society of Cariology and Endodontics,Chinese Stomatological Association.It addresses the current evidence to support the application of pulpotomy as a potential alternative to root canal treatment(RCT)on mature permanent teeth with pulpitis from a biological basis,the development of capping biomaterial,and the diagnostic considerations to evidence-based medicine.This expert statement intends to provide a clinical protocol of pulpotomy,which facilitates practitioners in choosing the optimal procedure and increasing their confidence in this rapidly evolving field.展开更多
Pulpitis is a common infective oral disease in clinical situations.The regulatory mechanisms of immune defense in pulpitis are still being investigated.Osteomodulin(OMD)is a small leucine-rich proteoglycan family memb...Pulpitis is a common infective oral disease in clinical situations.The regulatory mechanisms of immune defense in pulpitis are still being investigated.Osteomodulin(OMD)is a small leucine-rich proteoglycan family member distributed in bones and teeth.It is a bioactive protein that promotes osteogenesis and suppresses the apoptosis of human dental pulp stem cells(hDPSCs).In this study,the role of OMD in pulpitis and the OMD-induced regulatory mechanism were investigated.The OMD expression in normal and inflamed human pulp tissues was detected via immunofluorescence staining.Intriguingly,the OMD expression decreased in the inflammatory infiltration area of pulpitis specimens.The cellular experiments demonstrated that recombined human OMD could resist the detrimental effects of lipopolysaccharide(LPS)-induced inflammation.A conditional Omd knockout mouse model with pulpal inflammation was established.LPS-induced inflammatory impairment significantly increased in conditional Omd knockout mice,whereas OMD administration exhibited a protective effect against pulpitis.Mechanistically,the transcriptome alterations of OMD overexpression showed significant enrichment in the nuclear factor-κB(NF-κB)signaling pathway.Interleukin-1 receptor 1(IL1R1),a vital membrane receptor activating the NF-κB pathway,was significantly downregulated in OMD-overexpressing hDPSCs.Additionally,the interaction between OMD and IL1R1 was verified using co-immunoprecipitation and molecular docking.In vivo,excessive pulpal inflammation in Omd-deficient mice was rescued using an IL1R antagonist.Overall,OMD played a protective role in the inflammatory response via the IL1R1/NF-κB signaling pathway.OMD may optimize the immunomodulatory functions of hDPSCs and can be used for regenerative endodontics.展开更多
Dental pulp-dentin complex defects remain a major unresolved problem in oral medicines.Clinical therapeutic methods including root canal therapy and vital pulp therapy are both considered as conservative strategies,wh...Dental pulp-dentin complex defects remain a major unresolved problem in oral medicines.Clinical therapeutic methods including root canal therapy and vital pulp therapy are both considered as conservative strategies,which are incapable of repairing the pulpdentin complex defects.Although biomaterial-based strategies show remarkable progress in antibacterial,anti-inflammatory,and pulp regeneration,the important modulatory effects of nerves within pulp cavity have been greatly overlooked,making it challenging to achieve functional pulp-dentin complex regeneration.In this study,we propose an injectable bioceramicscontaining composite hydrogel in combination of Li-Ca-Si(LCS)bioceramics and gelatin methacrylate matrix with photocrosslinking properties.Due to the sustained release of bioactive Li,Ca and Si ions from LCS,the composite hydrogels possess multiple functions of promoting the neurogenic differentiation of Schwann cells,odontogenic differentiation of dental pulp stem cells,and neurogenesis-odontogenesis couples in vitro.In addition,the in vivo results showed that LCS-containing composite hydrogel can significantly promote the pulp-dentin complex repair.More importantly,LCS bioceramics-containing composite hydrogel can induce the growth of nerve fibers,leading to the re-innervation of pulp tissues.Taken together,the study suggests that LCS bioceramics can induce the innervation of pulp-dentin complex repair,offering a referable strategy of designing multifunctional filling materials for functional periodontal tissue regeneration.展开更多
In recent years,in response to the continuously rising demands of agricultural development,corn,as a key staple crop in China,plays an indispensable role in ensuring national food security.This study comprehensively e...In recent years,in response to the continuously rising demands of agricultural development,corn,as a key staple crop in China,plays an indispensable role in ensuring national food security.This study comprehensively evaluates the growth characteristics of various corn varieties and explores the practical benefits of high-yield cultivation strategies.The results show that a reasonable combination of corn varieties,scientific water and fertilizer management,and efficient pest and disease control measures can significantly increase corn yield.The findings of this study are crucial for accelerating the advancement of the corn industry in Nidang Town and its surrounding areas,while also providing valuable experience and technical support for achieving high-yield corn cultivation in similar regions.展开更多
Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with l...Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with less than 20%mass loss rate by bio-pulping synergistic treatment of straw fibers with deep eutectic solvent(DES)and Streptomyces rochei(S.rochei).Results illustrated that at 3%S.rochei concentration with 7-day fermentation,both cellulose and hemicellulose enzyme activities of the treated rice straw fiber reached peak values with a fiber mass loss rate of 17.01%.Microstructural morphology revealed that S.rochei colonization initiated on straw surfaces and progressively penetrated internal structures,resulting in surface loosening and distinct disruption of cell wall tissues within vascular bundles in transverse sections.The treated rice straw strip indicated a maximum tensile strength of 46.22 MPa for(Bacteria)BA 3%at day 7,attributed to optimized synergistic effects of microfibril angle(MFA)and cellulose/hemicellulose relative content ratio.The modified straw pulp film exhibited significant enhancement in the tensile index(44.9%increase),burst index(10.3%increase),and tear index(60%increase)compared to untreated groups.This work demonstrated the important role ofDES and S.rochei bio-pulping synergistic treatment in improving rice straw pulp performance,suggesting an eco-friendly,novel,and efficient biomass pretreatment technology for potential application prospects in sustainable agricultural mulching materials.展开更多
Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on t...Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on type 2 diabetes mellitus(T2DM)in mice induced by a high-fat diet(HFD)combined with streptozotocin(STZ).The results showed that PP alleviated hyperglycemia,hyperlipidemia,hyperuricemia,and tissue dysfunction in T2DM mice through the synergistic effect of nutrients and non-nutrient compounds.Analysis of mRNA expression levels revealed that PP improved glucose metabolism in T2DM mice by promoting glycogen synthesis and inhibiting gluconeogenesis.Furthermore,elevated levels of PP resulted in an increase in acetic acid content following a 4 weeks intervention period.Additionally,it led to the restoration of gut microbiota balance by decreasing the Firmicutes/Bacteroidota(F/B)ratio and enhancing the presence of Romboutsia,Allobaculum,Alloprevotella,and Bacteroides after an 8 weeks intervention.Ultimately,our results suggest that PP may offer advantages for individuals with diabetes.展开更多
Mapping potential areas for finfish mariculture,particularly high-yield regions,is crucial for the proper utilization of marine space and global food security.Physiological models(growth performance models)that consid...Mapping potential areas for finfish mariculture,particularly high-yield regions,is crucial for the proper utilization of marine space and global food security.Physiological models(growth performance models)that consider the spatiotemporal heterogeneity of the marine environment are a potentially effective approach to achieving this goal.In the present study,we developed an integrated model that combines the thermal performance curve and spatiotemporal heterogeneity of the marine environment to map the global high-yield potential mariculture areas for 27 commercial finfish species.Our results showed that the current sizes of the potentially suitable areas(achieving 50% of the maximum growth rate for at least six months annually)and high-yield areas(achieving 75% of the maximum growth rate throughout a year)are(8.00±0.30)×10^(6) and(5.96±0.13)×10^(6) km^(2),respectively.Currently,the sizes of suitable and high-yield areas for warm-water mariculture fish are larger than those for other species.The growth potential of suitable mariculture areas is higher at mid and low latitudes than at high latitudes.Under the two shared socioeconomic pathway scenarios(SSP1-2.6 and SSP5-8.5),the sizes of both suitable and high-yield areas will increase by 2050.However,there is the potential for finfish mariculture to respond differently to climate change among species and regions,and cold-water fish may benefit from global warming.Overall,the global potential for suitable high-yield mariculture areas continues to increase,making finfish mariculture an important contributor to global food security.展开更多
BACKGROUND The two-way,three-stage psychological intervention for patients with dental pulp disease offers a reference for nursing interventions in such cases.AIM To examine the effects of a three-stage psychological ...BACKGROUND The two-way,three-stage psychological intervention for patients with dental pulp disease offers a reference for nursing interventions in such cases.AIM To examine the effects of a three-stage psychological intervention on psychological resilience and health behaviors.METHODS A total of 114 patients with dental pulp disease treated between December 2022 and December 2023 were allocated into two groups according to the random lottery method,with 57 patients in each group.The control group adopted the teaching method,while the observation group used a three-stage psychological intervention combined with the teaching method.We compared psychological resilience,coping strategies,dental fear,health behavior habits,and stigma between the two groups.RESULTS The intervention group showed significantly improved scores on the psychological resilience scale(Connor-Davidson Resilience Scale)(P<0.05);positive and negative response scores also improved after the intervention(P<0.05);significant differences were observed between the observation and control groups in the Chinese version of the Stouthard Dental Fear Scale(Dental Anxiety Inventory),the Social Impact Scale,and health behavior score(P<0.05).CONCLUSION Combining a three-stage psychological intervention with the back-teaching method effectively reduces dental fear and stigma in patients with dental pulp disease.It also improves psychological resilience,coping strategies,and health behavior habits,achieving significant results.展开更多
Tooth pulpitis is a prevalent oral disorder.Understanding the underlying mechanisms of pulpitis and developing effective treatment strategies hold great significance.Ferroptosis has recently emerged as a new form of c...Tooth pulpitis is a prevalent oral disorder.Understanding the underlying mechanisms of pulpitis and developing effective treatment strategies hold great significance.Ferroptosis has recently emerged as a new form of cell death,but the role of ferroptosis in pulpitis remains largely unknown.In our study,single-cell RNA sequencing(sc RNA-seq)was used to identify cellular heterogeneity between 3 pulpitis tissue and 3 healthy pulp tissue,and explored ferroptosis occurrence in pulpitis tissue and inflamed dental pulp cells(DPCs).In scRNA-seq,40231 cells(Pulpitis:17814;Healthy pulp:22417)were captured,and visualized into 12 distinct cell clusters.Differential y expressed ferroptosis-related genes(DE-FRGs)were almost presented in each cluster in pulpitis vs healthy pulp.ROS and Fe^(2+)levels significantly rose,and immunohistochemistry showed low expression of GPX4 and high expression of PTGS2 in pulpitis.In LPSstimulated DPCs,thymosinα1 increased the expression of GPX4 and FTL,and decreased expression of TNF-α,IL-1β,IL-6,and Fe^(2+)levels.In rat pulpitis models,both prothymosinα(PTMA,precursor of thymosinα1)gelatin sponge placed at the hole of pulp(LPS-P(gs))and PTMA injection in pulp(LPS-P(i))significantly reduced infiltration of inflammatory cells and expression of PTGS2,and increased the expression of GPX4.In RNA sequencing,the expression of DE-FRGs were reversed when thymosinα1 were added in LPS-stimulated DPCs.Collectively,single-cell atlas reveals cellular heterogeneity between pulpitis and healthy pulp,and ferroptosis occurrence in pulpitis.Thymosinα1 may reduce ferroptosis in DPCs to alleviate pulpitis and thus potentially has the ability to treat pulpitis.展开更多
This study examines the development of loose-fill thermal insulation materials derived from annual plant residues,such as wheat straw,water reeds,and corn stalks,processed using the chemimechanical pulping(CMP)techniq...This study examines the development of loose-fill thermal insulation materials derived from annual plant residues,such as wheat straw,water reeds,and corn stalks,processed using the chemimechanical pulping(CMP)technique.The chopped plants were soda-cooked for 30 min,varying NaOH concentration(2%–8%on a dry basis of biomass),and mechanically refined using different disc types.The CMPprocess enhances the homogeneity and stability of defibratedmaterial,yielding improved insulation properties compared to untreated chopped rawmaterials.Chemical analysis revealed that CMP increases cellulose content and reduces lignin levels,enhancing water retention and vapor diffusion properties.Settlement tests confirmed that CMP materials are more resistant to compaction under vibration,maintaining long-term performance.Additionally,the CMP enables the production of lightweight materials that require less resource consumption while achieving comparable thermal insulation performance.The investigated biobased materials offer a sustainable alternative to conventional insulation,with competing thermal conductivity values(0.041-0.046 W/mK)at the settlement-resistant bulk density level of 60 kg/m^(3).The thermal conductivity of CMP materials remains minimally affected.However,the resulting fibers demonstrate significant advantages in stability and material efficiency.This highlights its suitability for loose-fill applications to improve the sustainability of the construction.Using renewable plant residues,CMP-based insulation materials align with circular economy principles and contribute to environmental sustainability.This research underscores the potential of CMP materials to reduce greenhouse gas emissions,optimize resource use,and promote eco-friendly building practices.展开更多
[Objective] The aim was to study on effects of N fertilizer on yield, N absorption and utilization of different cultivars of super high-yielding summer maize, in order to provide reference for reasonable N fertilizati...[Objective] The aim was to study on effects of N fertilizer on yield, N absorption and utilization of different cultivars of super high-yielding summer maize, in order to provide reference for reasonable N fertilization in accordance with different cultivars. [Method] Field experiment was conducted to study on effects of different N fertilizers on yield, N absorption and use efficiency of Zhengdan 958 and Xundan 20, in order to learn the effect differences at different N fertilizer levels. [Result] After N was applied, yields of the two summer maize increased significantly. Zhengdan 958 achieved the highest in yield and proceeds at 12 051.18 kg/hm2 and 1 722.40 yuan/hm2, respectively in low N level. In contrast, Xundan 20 achieved the highest at 13 166.00 kg/hm2 and 1 343.92 yuan/hm2 in the above two aspects in high N level. Compared with Zhengdan 958, Xundan 20 increased by 9.90%, 5.20% and 12.00% in N levels of 0, 240, and 450 kg/hm2, respectively. When N fertilizers were applied, protein yield of Xundan 20 was significantly higher than that of Zhengdan 958, so that higher N fertilizers contributed higher protein yield for Xundan 20. In high N level, N efficiency, N-fertilizer utilization and partial productivity of Xundan 20 were significantly higher than that of Zhengdan 958. [Conclusion] Lower N-fertilizer was suitable for Zhengdan 958 and Xundan 20 would get a good harvest if more N-fertilizers were applied. The results provided references for reasonable N fertilization.展开更多
Using a split plot design, super high-yielding early rice ＆quot;Zhongjiazao 17＆quot;and ＆quot;Ganxin 203＆quot; and super high-yielding late rice ＆quot;Ganxin 688＆quot; and ＆quot;Wufengy-ouT025＆quot; were taken...Using a split plot design, super high-yielding early rice ＆quot;Zhongjiazao 17＆quot;and ＆quot;Ganxin 203＆quot; and super high-yielding late rice ＆quot;Ganxin 688＆quot; and ＆quot;Wufengy-ouT025＆quot; were taken as materials with planting density the same at 312 000 hil s/hm2, to explore the effects of RS/IS （Ratio of row spacing to intrarow row spacing） on yield, top three leaves morphological features and its relationship of them in 2011 and 2012. The results showed that, whether early or late rice, biomass, accumula-tion and apparent utilizing rate of nitrogen were lower in RS/IS5.0 （40.0 cm ＆#215;8.0 cm） treatment, but yield of rice was higher in RS/IS2.0 （25.0 cm ＆#215;12.8 cm） treatment;grain numbers per spike of rice was more in RS/IS2.8 （30.0 cm＆#215;10.7 cm） treatment, and thousand-grain weight of rice was higher in RS/IS1.3 （20.0 cm＆#215;16.0 cm） treat-ment. For 4 varieties of early or late rice aforementioned, basal angle, opening an-gle and leaf area of 2nd leaf from top were larger in RS/IS5.0 treatment, and basal angle and opening angle were smal er in RS/IS2.0 treatment and leaf area smal er in RS/IS1.3 treatment.展开更多
In order to research environment parameters and physiological indices of high-quality and high-yield apple trees, two orchards with young and mature apples trees were investigated to explore structural parameter of ap...In order to research environment parameters and physiological indices of high-quality and high-yield apple trees, two orchards with young and mature apples trees were investigated to explore structural parameter of apple tree and community, and some physiological indices in fields and by room measurements. The results showed that tree height of high-quality orchard was in the range of 260 to 290 cm, branch angle in 70°-75°, and orchard coverage rate in 75%-94%, and the connec-tion rates between rows and trees were lower. Furthermore, the total branches of mature orchard reached 1.04 ×106 per hm2, while the young orchard was 8.79 ×105 per hm2; the leaves were thick and chlorophyl content was high, with SPAD value at 58.22. Additional y, the photosynthesis of the orchard was strong, and net photo-synthetic rate was 17.48-21.8 μmolCO2/（m2·s）. The proportions of lateral shoot of bearing part were 81% and 75% respectively.展开更多
Cationized pulp fibers （CPF） were prepared by the adsorption of a novel biodegradable cationic ester quaternary ammonium salt （31441） on bleached softwood kraft pulp fibers. The optimized conditions for the CPF pr...Cationized pulp fibers （CPF） were prepared by the adsorption of a novel biodegradable cationic ester quaternary ammonium salt （31441） on bleached softwood kraft pulp fibers. The optimized conditions for the CPF preparation were： 4% of 31441 （based on oven-dry pulp）, 80℃ and 30 rain. The CPF was characterized by FT-IR, SEM and XPS. Experimental results showed that the CPF improved the retention of precipitated calcium carbonate （PCC） filler significantly. With 0.9% CPF （based on oven-dry pulp）, the retention of PCC increased from 57.53% to 72.21%. The physical properties of paper were also slightly improved. The tensile strength and burst strength of the paper with CPF were higher than those with CPAM. CPF addition had no effect on the stock drainage.展开更多
[Objective] The aim was to select simplified high-yielding cultivation technology of rice. [Method] The research explored three methods of farmland prepara- tion, transplanting, and fertilization including shallow rot...[Objective] The aim was to select simplified high-yielding cultivation technology of rice. [Method] The research explored three methods of farmland prepara- tion, transplanting, and fertilization including shallow rotary tillage and deep ploughing, mechanical transplanting and hand transplanting, controlled-release fertilizer and quantitative fertilization in order to investigate effects of different treatment groups on rice characteristics, economic characteristics, as well as labor saving, yield increasing and profit improving. [Result] Rice increased by 596.4-809.55 kg/hm2, labor saving reached 120-135 per hm2, and profit improved in the range from 5 521.39 to 8 727.48 yuan/hm2 in the treatment groups by combinations of shallow rotary tillage, mechanical transplanting, and controlled-release fertilizer, of shallow rotary tillage, mechanical transplanting, and quantitative fertilization, and of deep ploughing, mechanical transplanting, and controlled release fertilizer. [Conclusion] The three models are of promising prospects in production.展开更多
[Objective] This study aimed to investigate the characteristics of high-yield soybean varieties.[Method] The agronomic traits of six high-yield soybean varieties and other 57 summer sowing ones were investigated from ...[Objective] This study aimed to investigate the characteristics of high-yield soybean varieties.[Method] The agronomic traits of six high-yield soybean varieties and other 57 summer sowing ones were investigated from 2007 to 2010.[Result] The higher-yield summer sowing soybean varieties should have higher plants （R=0.551 6＊）,more effective pods （R=0.739 7＊）,more seeds per pod （R=0.318 9）,more flowers per plant （＞130）,higher pod setting rate （＞52％）,smaller ratio of aborted pods （＜40％）,longer tap roots （more than 20 cm） and lateral roots （more than 15 cm）,shorter bottom nodes （the length of six bottom nodes should be less than 25 cm）,lower center of gravity of the plant （＜40 cm）,greater stem dry weight,higher dry matter transformation rate and continuously increasing seed weight in late seed filling stage.The ideal growth stage model for summer sowing soybean was that the durations between adjacent stages of sowing,seedling emergence,flowering,seed setting and maturity stage were 5,29-33,29-33,32-36 d,respectively.[Conclusion] This pattern could not only satisfy the ecological conditions for double cropping system,but also balance the full development of vegetative and reproductive growth,promote the yield formation of summer sowing soybean.展开更多
基金supported by the National Natural Science Foundation of China(32171347)the Foundation of Leading Talents from Shanghai Health Commission(2022XD038)+1 种基金Training Program for Research Physicians in Innovation,the Funda-mental Research Funds for the Central Universities(YG2023QNA23)Transforma-tion from shanghai hospital development center(SHDC2022CRD002).
文摘Stem cells play a crucial role in maintaining tissue regenerative capacity and homeostasis.However,mechanisms associated with stem cell senescence require further investigation.In this study,we conducted a proteomic analysis of human dental pulp stem cells(HDPSCs)obtained from individuals of various ages.Our findings showed that the expression of NUP62 was decreased in aged HDPSCs.We discovered that NUP62 alleviated senescence-associated phenotypes and enhanced differentiation potential both in vitro and in vivo.Conversely,the knocking down of NUP62 expression aggravated the senescence-associated phenotypes and impaired the proliferation and migration capacity of HDPSCs.Through RNA-sequence and decoding the epigenomic landscapes remodeled induced by NUP62 overexpression,we found that NUP62 helps alleviate senescence in HDPSCs by enhancing the nuclear transport of the transcription factor E2F1.This,in turn,stimulates the transcription of the epigenetic enzyme NSD2.Finally,the overexpression of NUP62 influences the H3K36me2 and H3K36me3 modifications of anti-aging genes(HMGA1,HMGA2,and SIRT6).Our results demonstrated that NUP62 regulates the fate of HDPSCs via NSD2-dependent epigenetic reprogramming.
基金supported by the National Natural Science Foundation of China(82170941 and 82370948 to Lu Zhang,82071110 and 82230029 to Zhi Chen)the National Key R&D Program of China(2018YFC1105100)。
文摘Pulpotomy,which belongs to vital pulp therapy,has become a strategy for managing pulpitis in recent decades.This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing long-term patient-centered outcomes.Pulpotomy is categorized into partial pulpotomy(PP),the removal of a partial segment of the coronal pulp tissue,and full pulpotomy(FP),the removal of whole coronal pulp,which is followed by applying the biomaterials onto the remaining pulp tissue and ultimately restoring the tooth.Procedural decisions for the amount of pulp tissue removal or retention depend on the diagnostic of pulp vitality,the overall treatment plan,the patient’s general health status,and pulp inflammation reassessment during operation.This statement represents the consensus of an expert committee convened by the Society of Cariology and Endodontics,Chinese Stomatological Association.It addresses the current evidence to support the application of pulpotomy as a potential alternative to root canal treatment(RCT)on mature permanent teeth with pulpitis from a biological basis,the development of capping biomaterial,and the diagnostic considerations to evidence-based medicine.This expert statement intends to provide a clinical protocol of pulpotomy,which facilitates practitioners in choosing the optimal procedure and increasing their confidence in this rapidly evolving field.
基金supported by grants from the National Natural Science Foundation of China (82071104)Science and Technology Commission of Shanghai Municipality (23XD1434200/22Y21901000)+9 种基金Shanghai Hospital Development Center(SHDC12022120)National Clinical Research Center for Oral Diseases (NCRCO2021-omics-07)Shanghai Clinical Research Center for Oral Diseases (19MC1910600)Major and Key Cultivation Projects of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine (JYZP006)Shanghai’s Top Priority Research Center (2022ZZ01017)CAMS Innovation Fund for Medical Sciences (2019-I2M-5-037)Fundamental research program funding of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine(JYZZ237)Eastern Talent Plan Leading Project (BJZH2024001)partly supported by the Shanghai Ninth People’s Hospital affiliated with Shanghai Jiao Tong University,School of Medicine(JYJC202223)Shanghai Key Laboratory of Translational Medicine on Ear and Nose diseases (14DZ2260300)
文摘Pulpitis is a common infective oral disease in clinical situations.The regulatory mechanisms of immune defense in pulpitis are still being investigated.Osteomodulin(OMD)is a small leucine-rich proteoglycan family member distributed in bones and teeth.It is a bioactive protein that promotes osteogenesis and suppresses the apoptosis of human dental pulp stem cells(hDPSCs).In this study,the role of OMD in pulpitis and the OMD-induced regulatory mechanism were investigated.The OMD expression in normal and inflamed human pulp tissues was detected via immunofluorescence staining.Intriguingly,the OMD expression decreased in the inflammatory infiltration area of pulpitis specimens.The cellular experiments demonstrated that recombined human OMD could resist the detrimental effects of lipopolysaccharide(LPS)-induced inflammation.A conditional Omd knockout mouse model with pulpal inflammation was established.LPS-induced inflammatory impairment significantly increased in conditional Omd knockout mice,whereas OMD administration exhibited a protective effect against pulpitis.Mechanistically,the transcriptome alterations of OMD overexpression showed significant enrichment in the nuclear factor-κB(NF-κB)signaling pathway.Interleukin-1 receptor 1(IL1R1),a vital membrane receptor activating the NF-κB pathway,was significantly downregulated in OMD-overexpressing hDPSCs.Additionally,the interaction between OMD and IL1R1 was verified using co-immunoprecipitation and molecular docking.In vivo,excessive pulpal inflammation in Omd-deficient mice was rescued using an IL1R antagonist.Overall,OMD played a protective role in the inflammatory response via the IL1R1/NF-κB signaling pathway.OMD may optimize the immunomodulatory functions of hDPSCs and can be used for regenerative endodontics.
基金funded by the National Key R&D Program of China(2022YFC2405904)the National Natural Science Foundation of China(52272284,32225028)+1 种基金Joint Research Unit Plan of the Chinese Academy of Sciences(121631ZYLH20240014)the Science and Technology Commission of Shanghai Municipality(24520750100)。
文摘Dental pulp-dentin complex defects remain a major unresolved problem in oral medicines.Clinical therapeutic methods including root canal therapy and vital pulp therapy are both considered as conservative strategies,which are incapable of repairing the pulpdentin complex defects.Although biomaterial-based strategies show remarkable progress in antibacterial,anti-inflammatory,and pulp regeneration,the important modulatory effects of nerves within pulp cavity have been greatly overlooked,making it challenging to achieve functional pulp-dentin complex regeneration.In this study,we propose an injectable bioceramicscontaining composite hydrogel in combination of Li-Ca-Si(LCS)bioceramics and gelatin methacrylate matrix with photocrosslinking properties.Due to the sustained release of bioactive Li,Ca and Si ions from LCS,the composite hydrogels possess multiple functions of promoting the neurogenic differentiation of Schwann cells,odontogenic differentiation of dental pulp stem cells,and neurogenesis-odontogenesis couples in vitro.In addition,the in vivo results showed that LCS-containing composite hydrogel can significantly promote the pulp-dentin complex repair.More importantly,LCS bioceramics-containing composite hydrogel can induce the growth of nerve fibers,leading to the re-innervation of pulp tissues.Taken together,the study suggests that LCS bioceramics can induce the innervation of pulp-dentin complex repair,offering a referable strategy of designing multifunctional filling materials for functional periodontal tissue regeneration.
文摘In recent years,in response to the continuously rising demands of agricultural development,corn,as a key staple crop in China,plays an indispensable role in ensuring national food security.This study comprehensively evaluates the growth characteristics of various corn varieties and explores the practical benefits of high-yield cultivation strategies.The results show that a reasonable combination of corn varieties,scientific water and fertilizer management,and efficient pest and disease control measures can significantly increase corn yield.The findings of this study are crucial for accelerating the advancement of the corn industry in Nidang Town and its surrounding areas,while also providing valuable experience and technical support for achieving high-yield corn cultivation in similar regions.
基金funded by the Agricultural Science and Technology Innovation Fund of Jiangsu Province[Grant/Award Number:CX(24)1008]Key Research and Development Program Project of Henan Province(251111110100)National State Science Foundation of China[Grant/Award Number:21808093].
文摘Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with less than 20%mass loss rate by bio-pulping synergistic treatment of straw fibers with deep eutectic solvent(DES)and Streptomyces rochei(S.rochei).Results illustrated that at 3%S.rochei concentration with 7-day fermentation,both cellulose and hemicellulose enzyme activities of the treated rice straw fiber reached peak values with a fiber mass loss rate of 17.01%.Microstructural morphology revealed that S.rochei colonization initiated on straw surfaces and progressively penetrated internal structures,resulting in surface loosening and distinct disruption of cell wall tissues within vascular bundles in transverse sections.The treated rice straw strip indicated a maximum tensile strength of 46.22 MPa for(Bacteria)BA 3%at day 7,attributed to optimized synergistic effects of microfibril angle(MFA)and cellulose/hemicellulose relative content ratio.The modified straw pulp film exhibited significant enhancement in the tensile index(44.9%increase),burst index(10.3%increase),and tear index(60%increase)compared to untreated groups.This work demonstrated the important role ofDES and S.rochei bio-pulping synergistic treatment in improving rice straw pulp performance,suggesting an eco-friendly,novel,and efficient biomass pretreatment technology for potential application prospects in sustainable agricultural mulching materials.
基金supported by the Zhejiang Key R&D Program of China(2020C02037)the Ningbo Agricultural Research Program of China(2022S1542022S138).
文摘Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on type 2 diabetes mellitus(T2DM)in mice induced by a high-fat diet(HFD)combined with streptozotocin(STZ).The results showed that PP alleviated hyperglycemia,hyperlipidemia,hyperuricemia,and tissue dysfunction in T2DM mice through the synergistic effect of nutrients and non-nutrient compounds.Analysis of mRNA expression levels revealed that PP improved glucose metabolism in T2DM mice by promoting glycogen synthesis and inhibiting gluconeogenesis.Furthermore,elevated levels of PP resulted in an increase in acetic acid content following a 4 weeks intervention period.Additionally,it led to the restoration of gut microbiota balance by decreasing the Firmicutes/Bacteroidota(F/B)ratio and enhancing the presence of Romboutsia,Allobaculum,Alloprevotella,and Bacteroides after an 8 weeks intervention.Ultimately,our results suggest that PP may offer advantages for individuals with diabetes.
基金supported by the National Natural Science Founda-tion of China(42025604)the Fundamental Research Funds for the Central Universities of the Ocean University of China.
文摘Mapping potential areas for finfish mariculture,particularly high-yield regions,is crucial for the proper utilization of marine space and global food security.Physiological models(growth performance models)that consider the spatiotemporal heterogeneity of the marine environment are a potentially effective approach to achieving this goal.In the present study,we developed an integrated model that combines the thermal performance curve and spatiotemporal heterogeneity of the marine environment to map the global high-yield potential mariculture areas for 27 commercial finfish species.Our results showed that the current sizes of the potentially suitable areas(achieving 50% of the maximum growth rate for at least six months annually)and high-yield areas(achieving 75% of the maximum growth rate throughout a year)are(8.00±0.30)×10^(6) and(5.96±0.13)×10^(6) km^(2),respectively.Currently,the sizes of suitable and high-yield areas for warm-water mariculture fish are larger than those for other species.The growth potential of suitable mariculture areas is higher at mid and low latitudes than at high latitudes.Under the two shared socioeconomic pathway scenarios(SSP1-2.6 and SSP5-8.5),the sizes of both suitable and high-yield areas will increase by 2050.However,there is the potential for finfish mariculture to respond differently to climate change among species and regions,and cold-water fish may benefit from global warming.Overall,the global potential for suitable high-yield mariculture areas continues to increase,making finfish mariculture an important contributor to global food security.
基金ORCID number:Qing-Feng Wang 0009-0009-4579-7133Ying Wu 0009-0007-2061-739X。
文摘BACKGROUND The two-way,three-stage psychological intervention for patients with dental pulp disease offers a reference for nursing interventions in such cases.AIM To examine the effects of a three-stage psychological intervention on psychological resilience and health behaviors.METHODS A total of 114 patients with dental pulp disease treated between December 2022 and December 2023 were allocated into two groups according to the random lottery method,with 57 patients in each group.The control group adopted the teaching method,while the observation group used a three-stage psychological intervention combined with the teaching method.We compared psychological resilience,coping strategies,dental fear,health behavior habits,and stigma between the two groups.RESULTS The intervention group showed significantly improved scores on the psychological resilience scale(Connor-Davidson Resilience Scale)(P<0.05);positive and negative response scores also improved after the intervention(P<0.05);significant differences were observed between the observation and control groups in the Chinese version of the Stouthard Dental Fear Scale(Dental Anxiety Inventory),the Social Impact Scale,and health behavior score(P<0.05).CONCLUSION Combining a three-stage psychological intervention with the back-teaching method effectively reduces dental fear and stigma in patients with dental pulp disease.It also improves psychological resilience,coping strategies,and health behavior habits,achieving significant results.
基金supported through funding from Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515030036)the National Natural Science Foundation of China(Grant No.82270973)。
文摘Tooth pulpitis is a prevalent oral disorder.Understanding the underlying mechanisms of pulpitis and developing effective treatment strategies hold great significance.Ferroptosis has recently emerged as a new form of cell death,but the role of ferroptosis in pulpitis remains largely unknown.In our study,single-cell RNA sequencing(sc RNA-seq)was used to identify cellular heterogeneity between 3 pulpitis tissue and 3 healthy pulp tissue,and explored ferroptosis occurrence in pulpitis tissue and inflamed dental pulp cells(DPCs).In scRNA-seq,40231 cells(Pulpitis:17814;Healthy pulp:22417)were captured,and visualized into 12 distinct cell clusters.Differential y expressed ferroptosis-related genes(DE-FRGs)were almost presented in each cluster in pulpitis vs healthy pulp.ROS and Fe^(2+)levels significantly rose,and immunohistochemistry showed low expression of GPX4 and high expression of PTGS2 in pulpitis.In LPSstimulated DPCs,thymosinα1 increased the expression of GPX4 and FTL,and decreased expression of TNF-α,IL-1β,IL-6,and Fe^(2+)levels.In rat pulpitis models,both prothymosinα(PTMA,precursor of thymosinα1)gelatin sponge placed at the hole of pulp(LPS-P(gs))and PTMA injection in pulp(LPS-P(i))significantly reduced infiltration of inflammatory cells and expression of PTGS2,and increased the expression of GPX4.In RNA sequencing,the expression of DE-FRGs were reversed when thymosinα1 were added in LPS-stimulated DPCs.Collectively,single-cell atlas reveals cellular heterogeneity between pulpitis and healthy pulp,and ferroptosis occurrence in pulpitis.Thymosinα1 may reduce ferroptosis in DPCs to alleviate pulpitis and thus potentially has the ability to treat pulpitis.
基金funded by the LatvianCouncil of Science,the project“Investigation of Eco-Friendly Thermal Insulation Materials from Sustainable and Renewable Industrial Crops Residuals,”number lzp-2021/1-0599.
文摘This study examines the development of loose-fill thermal insulation materials derived from annual plant residues,such as wheat straw,water reeds,and corn stalks,processed using the chemimechanical pulping(CMP)technique.The chopped plants were soda-cooked for 30 min,varying NaOH concentration(2%–8%on a dry basis of biomass),and mechanically refined using different disc types.The CMPprocess enhances the homogeneity and stability of defibratedmaterial,yielding improved insulation properties compared to untreated chopped rawmaterials.Chemical analysis revealed that CMP increases cellulose content and reduces lignin levels,enhancing water retention and vapor diffusion properties.Settlement tests confirmed that CMP materials are more resistant to compaction under vibration,maintaining long-term performance.Additionally,the CMP enables the production of lightweight materials that require less resource consumption while achieving comparable thermal insulation performance.The investigated biobased materials offer a sustainable alternative to conventional insulation,with competing thermal conductivity values(0.041-0.046 W/mK)at the settlement-resistant bulk density level of 60 kg/m^(3).The thermal conductivity of CMP materials remains minimally affected.However,the resulting fibers demonstrate significant advantages in stability and material efficiency.This highlights its suitability for loose-fill applications to improve the sustainability of the construction.Using renewable plant residues,CMP-based insulation materials align with circular economy principles and contribute to environmental sustainability.This research underscores the potential of CMP materials to reduce greenhouse gas emissions,optimize resource use,and promote eco-friendly building practices.
基金Supported by Cooperation project of International Plant Nutrition Institution(IPNI)(NMBF-HenanAU-2007)Special Fund for Construction of National Modern Maize Industrial Technology System(nycytx-02-17)~~
文摘[Objective] The aim was to study on effects of N fertilizer on yield, N absorption and utilization of different cultivars of super high-yielding summer maize, in order to provide reference for reasonable N fertilization in accordance with different cultivars. [Method] Field experiment was conducted to study on effects of different N fertilizers on yield, N absorption and use efficiency of Zhengdan 958 and Xundan 20, in order to learn the effect differences at different N fertilizer levels. [Result] After N was applied, yields of the two summer maize increased significantly. Zhengdan 958 achieved the highest in yield and proceeds at 12 051.18 kg/hm2 and 1 722.40 yuan/hm2, respectively in low N level. In contrast, Xundan 20 achieved the highest at 13 166.00 kg/hm2 and 1 343.92 yuan/hm2 in the above two aspects in high N level. Compared with Zhengdan 958, Xundan 20 increased by 9.90%, 5.20% and 12.00% in N levels of 0, 240, and 450 kg/hm2, respectively. When N fertilizers were applied, protein yield of Xundan 20 was significantly higher than that of Zhengdan 958, so that higher N fertilizers contributed higher protein yield for Xundan 20. In high N level, N efficiency, N-fertilizer utilization and partial productivity of Xundan 20 were significantly higher than that of Zhengdan 958. [Conclusion] Lower N-fertilizer was suitable for Zhengdan 958 and Xundan 20 would get a good harvest if more N-fertilizers were applied. The results provided references for reasonable N fertilization.
基金Supported by National Key Technology Research and Development Program of China(2012BAD15B03)the Youth Innovation Fund of Jiangxi Academy of Agricultural Sciences(2010-CQN004)~~
文摘Using a split plot design, super high-yielding early rice ＆quot;Zhongjiazao 17＆quot;and ＆quot;Ganxin 203＆quot; and super high-yielding late rice ＆quot;Ganxin 688＆quot; and ＆quot;Wufengy-ouT025＆quot; were taken as materials with planting density the same at 312 000 hil s/hm2, to explore the effects of RS/IS （Ratio of row spacing to intrarow row spacing） on yield, top three leaves morphological features and its relationship of them in 2011 and 2012. The results showed that, whether early or late rice, biomass, accumula-tion and apparent utilizing rate of nitrogen were lower in RS/IS5.0 （40.0 cm ＆#215;8.0 cm） treatment, but yield of rice was higher in RS/IS2.0 （25.0 cm ＆#215;12.8 cm） treatment;grain numbers per spike of rice was more in RS/IS2.8 （30.0 cm＆#215;10.7 cm） treatment, and thousand-grain weight of rice was higher in RS/IS1.3 （20.0 cm＆#215;16.0 cm） treat-ment. For 4 varieties of early or late rice aforementioned, basal angle, opening an-gle and leaf area of 2nd leaf from top were larger in RS/IS5.0 treatment, and basal angle and opening angle were smal er in RS/IS2.0 treatment and leaf area smal er in RS/IS1.3 treatment.
基金Supported by Special Fund for Modern Agricultural Industry Technology System(CARS-28)~~
文摘In order to research environment parameters and physiological indices of high-quality and high-yield apple trees, two orchards with young and mature apples trees were investigated to explore structural parameter of apple tree and community, and some physiological indices in fields and by room measurements. The results showed that tree height of high-quality orchard was in the range of 260 to 290 cm, branch angle in 70°-75°, and orchard coverage rate in 75%-94%, and the connec-tion rates between rows and trees were lower. Furthermore, the total branches of mature orchard reached 1.04 ×106 per hm2, while the young orchard was 8.79 ×105 per hm2; the leaves were thick and chlorophyl content was high, with SPAD value at 58.22. Additional y, the photosynthesis of the orchard was strong, and net photo-synthetic rate was 17.48-21.8 μmolCO2/（m2·s）. The proportions of lateral shoot of bearing part were 81% and 75% respectively.
基金the Key Scientific and Technological Project of Heilongjiang Province (GB06B501-1)
文摘Cationized pulp fibers （CPF） were prepared by the adsorption of a novel biodegradable cationic ester quaternary ammonium salt （31441） on bleached softwood kraft pulp fibers. The optimized conditions for the CPF preparation were： 4% of 31441 （based on oven-dry pulp）, 80℃ and 30 rain. The CPF was characterized by FT-IR, SEM and XPS. Experimental results showed that the CPF improved the retention of precipitated calcium carbonate （PCC） filler significantly. With 0.9% CPF （based on oven-dry pulp）, the retention of PCC increased from 57.53% to 72.21%. The physical properties of paper were also slightly improved. The tensile strength and burst strength of the paper with CPF were higher than those with CPAM. CPF addition had no effect on the stock drainage.
文摘[Objective] The aim was to select simplified high-yielding cultivation technology of rice. [Method] The research explored three methods of farmland prepara- tion, transplanting, and fertilization including shallow rotary tillage and deep ploughing, mechanical transplanting and hand transplanting, controlled-release fertilizer and quantitative fertilization in order to investigate effects of different treatment groups on rice characteristics, economic characteristics, as well as labor saving, yield increasing and profit improving. [Result] Rice increased by 596.4-809.55 kg/hm2, labor saving reached 120-135 per hm2, and profit improved in the range from 5 521.39 to 8 727.48 yuan/hm2 in the treatment groups by combinations of shallow rotary tillage, mechanical transplanting, and controlled-release fertilizer, of shallow rotary tillage, mechanical transplanting, and quantitative fertilization, and of deep ploughing, mechanical transplanting, and controlled release fertilizer. [Conclusion] The three models are of promising prospects in production.
文摘[Objective] This study aimed to investigate the characteristics of high-yield soybean varieties.[Method] The agronomic traits of six high-yield soybean varieties and other 57 summer sowing ones were investigated from 2007 to 2010.[Result] The higher-yield summer sowing soybean varieties should have higher plants （R=0.551 6＊）,more effective pods （R=0.739 7＊）,more seeds per pod （R=0.318 9）,more flowers per plant （＞130）,higher pod setting rate （＞52％）,smaller ratio of aborted pods （＜40％）,longer tap roots （more than 20 cm） and lateral roots （more than 15 cm）,shorter bottom nodes （the length of six bottom nodes should be less than 25 cm）,lower center of gravity of the plant （＜40 cm）,greater stem dry weight,higher dry matter transformation rate and continuously increasing seed weight in late seed filling stage.The ideal growth stage model for summer sowing soybean was that the durations between adjacent stages of sowing,seedling emergence,flowering,seed setting and maturity stage were 5,29-33,29-33,32-36 d,respectively.[Conclusion] This pattern could not only satisfy the ecological conditions for double cropping system,but also balance the full development of vegetative and reproductive growth,promote the yield formation of summer sowing soybean.
