Objective] The study aimed to optimize the procedures for flavonoids ex-traction from of Lophatherum gracile. [Method] The powder of L. gracile leaf （3.0 g） was weighed and extracted fol owing an orthogonal design i...Objective] The study aimed to optimize the procedures for flavonoids ex-traction from of Lophatherum gracile. [Method] The powder of L. gracile leaf （3.0 g） was weighed and extracted fol owing an orthogonal design including the solid-liquid ratio, extraction time, extraction times and soaking time at three levels. [Result] The optimal parameters were solid-liquid ratio at 1：40, extraction time of 40 s, extraction times of two times and soaking time of 40 min. [Conclusion] Smashing tissue ex-traction of flavonoids is rapid and efficient, which provides a new method for the development and utilization of L. gracile.展开更多
[Objectives] In order to explore the effect of fermentation on Lophatherum gracile Brongn. [Methods] The single factor experiment was applied to screen the fermentation conditions of L. gracile Brongn,and the effect o...[Objectives] In order to explore the effect of fermentation on Lophatherum gracile Brongn. [Methods] The single factor experiment was applied to screen the fermentation conditions of L. gracile Brongn,and the effect of fermentation on the contents of total flavonoids and total polysaccharides was explored. [Results] The experiment defined the best fermentation conditions as: keeping natural state of p H,temperature 35℃,inoculation amount 6%,rotation speed 210 r/min,fermentation time 36 h. The results showed that the content increase of total flavonoids in the fermentation group was 5. 59% higher than the that in the non-fermentation group. [Conclusions]The selected fermentation method could improve the contents of total flavonoids and total polysaccharides.展开更多
Neurons in the gracile nucleus of the camel brain stem were studied by Golgi method. Neurons were classified based on soma size and shape, density of dendritic tree, morphology and distribution of appendages. Six type...Neurons in the gracile nucleus of the camel brain stem were studied by Golgi method. Neurons were classified based on soma size and shape, density of dendritic tree, morphology and distribution of appendages. Six types of neurons were identified. Type I neurons had very large somata with appendages on their somata and distal dendrites. Type II neurons had large somata and almost smooth dendrites. Type III neurons displayed medium size somata with dendritic appendages of different forms. Type IV neurons were small to medium spheroidal or triangular neurons. Somata and dendrites of these neurons had appendages of different forms. Type V medium-size neurons had bipolar, round or fusiform somata and poorly branching dendritic tree. Some spines and appendages were seen on somata and dendrites of these neurons. Neurons of type VI were medium-size unipolar, round or fusiform with spines on their dendrites. The radiating branching pattern was more common than the tufted for all types of neurons. Wide overlap between widths of dendrites of different orders was found for all neuronal types. Hopefully, this study will improve our understanding of the role of the gracile nucleus as major center for sensory information processing and contribute to our understanding of comparative neuroanatomy and neurophysiology.展开更多
Lophatherum gracile extract(LG)was taken as the research object,and evaluated respectively from two dimensions of efficacy and safety,so as to enhance its application prospect in the field of cosmetics.Eye irritation ...Lophatherum gracile extract(LG)was taken as the research object,and evaluated respectively from two dimensions of efficacy and safety,so as to enhance its application prospect in the field of cosmetics.Eye irritation and skin irritation were evaluated by chick embryo chorioallantoic membrane experiment(HET-CAM)and human patch test.The transient potential receptor vanillate subtype 1(TRPV1)was highly expressed in the cell model,and the influence of the expression of interleukin 8(IL-8)and monocyte chemotactic factor(MCP-1)were measured.Sensitivity skin stimulation models were established using sodium dodecyl sulfate,lactic acid and capsaicin and to determine its ability to relieve skin sensitivity.HET-CAM and human patch test show that LG has good eye safety and skin safety.In the cell model with over-expression of TRPV1,LG can reduce the over-expression of IL-8 and MCP-1 in a dose-dependent manner.In human evaluation,LG can significantly reduce the transepidermal water loss rate(TEWL)and skin erythema index(EI)increase caused by SDS(p<0.05),instantly alleviate skin irritation caused by lactic acid stimulation,significantly reduce skin discomfort and redness caused by capsaicin stimulation,indicating that LG can accelerate the repair of damaged skin and enhance the barrier function of sensitive skin(p<0.05).The results of Therefore,as an active raw material of plant origin,LG has the characteristics of high efficiency,strong effect and safety,which can be widely used in the development of skin care product.展开更多
Lophatheri Herba(Danzhuye in Chinese)is derived from the dried stems and leaves of Lophatherum gracile and has a long history of use as a medicinal and food source.Flavonoids and phenolic acids are the main active ing...Lophatheri Herba(Danzhuye in Chinese)is derived from the dried stems and leaves of Lophatherum gracile and has a long history of use as a medicinal and food source.Flavonoids and phenolic acids are the main active ingredients in Lophatheri Herba,which produce diuretic,anti-inflammatory,and antipyretic effects.Flavonoid glycosides and hydroxybenzoic acids are respectively the main structure in 44 flavonoids and 16 phenolic acids obtained from Lophatheri Herba.Modern pharmacological studies have found that the main chemical constituents of Lophatheri Herba play important roles in anti-inflammatory,cardioprotective,hepatoprotective and hypoglycaemic effects.Studies have demonstrated that flavonoid monomers,for example,luteolin,isoorientin,luteolin-7-O-β-D-glucoside and apigenin are more effective in exerting the above pharmacological effects.In addition,Lophatheri Herba is used in different food products as the main ingredient or as an accessory.This review describes Lophatheri Herba in terms of its chemical composition,pharmacological effects and efficacy,food development and applications,and clinical utility,and discusses the problems facing its use.This study provides valuable ideas and a scientific basis for the future development and use of L.gracile.展开更多
基金Supported by Special Fund for Scientific and Technological Innovation of Henan University of Traditional Chinese Medicine(2012PYTD03)~~
文摘Objective] The study aimed to optimize the procedures for flavonoids ex-traction from of Lophatherum gracile. [Method] The powder of L. gracile leaf （3.0 g） was weighed and extracted fol owing an orthogonal design including the solid-liquid ratio, extraction time, extraction times and soaking time at three levels. [Result] The optimal parameters were solid-liquid ratio at 1：40, extraction time of 40 s, extraction times of two times and soaking time of 40 min. [Conclusion] Smashing tissue ex-traction of flavonoids is rapid and efficient, which provides a new method for the development and utilization of L. gracile.
基金Sponsored by National Scientific and Technological Special Fund:Rescue and Sorting of Traditional Chinese Herbal and Veterinary Resources(2013FY110600-03)
文摘[Objectives] In order to explore the effect of fermentation on Lophatherum gracile Brongn. [Methods] The single factor experiment was applied to screen the fermentation conditions of L. gracile Brongn,and the effect of fermentation on the contents of total flavonoids and total polysaccharides was explored. [Results] The experiment defined the best fermentation conditions as: keeping natural state of p H,temperature 35℃,inoculation amount 6%,rotation speed 210 r/min,fermentation time 36 h. The results showed that the content increase of total flavonoids in the fermentation group was 5. 59% higher than the that in the non-fermentation group. [Conclusions]The selected fermentation method could improve the contents of total flavonoids and total polysaccharides.
文摘Neurons in the gracile nucleus of the camel brain stem were studied by Golgi method. Neurons were classified based on soma size and shape, density of dendritic tree, morphology and distribution of appendages. Six types of neurons were identified. Type I neurons had very large somata with appendages on their somata and distal dendrites. Type II neurons had large somata and almost smooth dendrites. Type III neurons displayed medium size somata with dendritic appendages of different forms. Type IV neurons were small to medium spheroidal or triangular neurons. Somata and dendrites of these neurons had appendages of different forms. Type V medium-size neurons had bipolar, round or fusiform somata and poorly branching dendritic tree. Some spines and appendages were seen on somata and dendrites of these neurons. Neurons of type VI were medium-size unipolar, round or fusiform with spines on their dendrites. The radiating branching pattern was more common than the tufted for all types of neurons. Wide overlap between widths of dendrites of different orders was found for all neuronal types. Hopefully, this study will improve our understanding of the role of the gracile nucleus as major center for sensory information processing and contribute to our understanding of comparative neuroanatomy and neurophysiology.
文摘Lophatherum gracile extract(LG)was taken as the research object,and evaluated respectively from two dimensions of efficacy and safety,so as to enhance its application prospect in the field of cosmetics.Eye irritation and skin irritation were evaluated by chick embryo chorioallantoic membrane experiment(HET-CAM)and human patch test.The transient potential receptor vanillate subtype 1(TRPV1)was highly expressed in the cell model,and the influence of the expression of interleukin 8(IL-8)and monocyte chemotactic factor(MCP-1)were measured.Sensitivity skin stimulation models were established using sodium dodecyl sulfate,lactic acid and capsaicin and to determine its ability to relieve skin sensitivity.HET-CAM and human patch test show that LG has good eye safety and skin safety.In the cell model with over-expression of TRPV1,LG can reduce the over-expression of IL-8 and MCP-1 in a dose-dependent manner.In human evaluation,LG can significantly reduce the transepidermal water loss rate(TEWL)and skin erythema index(EI)increase caused by SDS(p<0.05),instantly alleviate skin irritation caused by lactic acid stimulation,significantly reduce skin discomfort and redness caused by capsaicin stimulation,indicating that LG can accelerate the repair of damaged skin and enhance the barrier function of sensitive skin(p<0.05).The results of Therefore,as an active raw material of plant origin,LG has the characteristics of high efficiency,strong effect and safety,which can be widely used in the development of skin care product.
基金supported by National Key Research and Development Plan Project(No.2022YFC3501805)。
文摘Lophatheri Herba(Danzhuye in Chinese)is derived from the dried stems and leaves of Lophatherum gracile and has a long history of use as a medicinal and food source.Flavonoids and phenolic acids are the main active ingredients in Lophatheri Herba,which produce diuretic,anti-inflammatory,and antipyretic effects.Flavonoid glycosides and hydroxybenzoic acids are respectively the main structure in 44 flavonoids and 16 phenolic acids obtained from Lophatheri Herba.Modern pharmacological studies have found that the main chemical constituents of Lophatheri Herba play important roles in anti-inflammatory,cardioprotective,hepatoprotective and hypoglycaemic effects.Studies have demonstrated that flavonoid monomers,for example,luteolin,isoorientin,luteolin-7-O-β-D-glucoside and apigenin are more effective in exerting the above pharmacological effects.In addition,Lophatheri Herba is used in different food products as the main ingredient or as an accessory.This review describes Lophatheri Herba in terms of its chemical composition,pharmacological effects and efficacy,food development and applications,and clinical utility,and discusses the problems facing its use.This study provides valuable ideas and a scientific basis for the future development and use of L.gracile.
