The gp37 gene from LsMNPV has been sequenced and the deduced amino acid sequence was compared with other GP37 amino acid sequences from 8 insect viruses. The maximum homology of amino acid sequences and the conserved ...The gp37 gene from LsMNPV has been sequenced and the deduced amino acid sequence was compared with other GP37 amino acid sequences from 8 insect viruses. The maximum homology of amino acid sequences and the conserved structural regions were analyzed with PROSIS software. The relationship of evolution of 9 insect viruses was discussed and the evolutionary tree was drawned.展开更多
The increasing emergence of multi-drug resistant Escherichia coli(E.coli)has become a global concern,primarily due to the limitation of antimicrobial treatment options.Phage therapy has been considered as a promising ...The increasing emergence of multi-drug resistant Escherichia coli(E.coli)has become a global concern,primarily due to the limitation of antimicrobial treatment options.Phage therapy has been considered as a promising alternative for treating infections caused by multi-drug resistant E.coli.However,the application of phages as a promising antimicrobial agent is limited by their narrow host range and specificity.In this research,a recombinant T4-like phage,named WGqlae,has been obtained by changing the receptor specificity determinant region of gene 37,using a homologous recombination platform of T4-like phages established by our laboratory previously.The engineered phage WGqlae can lyse four additional hosts,comparing to its parental phages WG01 and QL01.WGqlae showed similar characteristics,including thermo and pH stability,optimal multiplicity of infection and one-step growth curve,to the donor phage QL01.In addition,sequencing results showed that gene 37 of recombinant phage WGqlae had genetically stable even after 20 generations.In planktonic test,phage WGqlae had significant antimicrobial effects on E.coli DE192 and DE205 B.The optical density at 600 nm(OD600)of E.coli in phage WGqlae treating group was significantly lower than that of the control group(P\0.01).Besides,phage WGqlae demonstrated an obvious inhibitory effect on the biofilm formation and the clearance of mature biofilms.Our study suggested that engineered phages may be promising candidates for future phage therapy applications against pathogenic E.coli in planktonic and biofilm forms.展开更多
Blood samples were collected from a local strain of chickens associated with serious tumor cases in Shandong Province. The samples were inoculated into chicken embryo fibroblast and DF-1 cells for virus isolation and ...Blood samples were collected from a local strain of chickens associated with serious tumor cases in Shandong Province. The samples were inoculated into chicken embryo fibroblast and DF-1 cells for virus isolation and identification, respectively. The inoculated cells were screened for three common chicken tumor viruses. Nine strains of avian leukosis virus subgroup J （ALV-J） were identified, and were designated LY1201-LYI209. The env gene from the LY1201 strain was amplified and cloned. All nine resultant env clones （clones 01-09） were sequenced, and the gp85 and gp37 amino acid regions were subjected to homology analysis. Clones 01 and 03 had 10 amino acid deletions in the gp85 region compared to the other seven clones, suggesting that at least two quasispecies with obvious mutations coexist in the same field strain. Among these nine clones, three had identical gp85 and gp37 sequences, and were recognized as the dominant LY1201 quasispecies. The amino acid sequence homology of gp37 and gp85 among the nine clones was 98.5%-100.0% and 96.6%-100.0% respectively, suggesting that the gp85 region of the env gene can better display the quasispecies diversity of ALV-J than gp37.展开更多
文摘The gp37 gene from LsMNPV has been sequenced and the deduced amino acid sequence was compared with other GP37 amino acid sequences from 8 insect viruses. The maximum homology of amino acid sequences and the conserved structural regions were analyzed with PROSIS software. The relationship of evolution of 9 insect viruses was discussed and the evolutionary tree was drawned.
基金supported by Grants from the National Natural Science Foundation of China(U1803109)Key research and development plan of Jiangsu province(BE2019304)+2 种基金National Key R&D Program of China(2018YFC1602500)the Central University Basic Scientific Research Fund-Animal pathogenic bacteria(KYZ201846)Jiangsu modern agriculture(waterfowl)industrial technology system disease prevention and control innovation team(JATS[2018]222)
文摘The increasing emergence of multi-drug resistant Escherichia coli(E.coli)has become a global concern,primarily due to the limitation of antimicrobial treatment options.Phage therapy has been considered as a promising alternative for treating infections caused by multi-drug resistant E.coli.However,the application of phages as a promising antimicrobial agent is limited by their narrow host range and specificity.In this research,a recombinant T4-like phage,named WGqlae,has been obtained by changing the receptor specificity determinant region of gene 37,using a homologous recombination platform of T4-like phages established by our laboratory previously.The engineered phage WGqlae can lyse four additional hosts,comparing to its parental phages WG01 and QL01.WGqlae showed similar characteristics,including thermo and pH stability,optimal multiplicity of infection and one-step growth curve,to the donor phage QL01.In addition,sequencing results showed that gene 37 of recombinant phage WGqlae had genetically stable even after 20 generations.In planktonic test,phage WGqlae had significant antimicrobial effects on E.coli DE192 and DE205 B.The optical density at 600 nm(OD600)of E.coli in phage WGqlae treating group was significantly lower than that of the control group(P\0.01).Besides,phage WGqlae demonstrated an obvious inhibitory effect on the biofilm formation and the clearance of mature biofilms.Our study suggested that engineered phages may be promising candidates for future phage therapy applications against pathogenic E.coli in planktonic and biofilm forms.
基金financially supported by a special grant from Ministry of Agriculture of China in 2012
文摘Blood samples were collected from a local strain of chickens associated with serious tumor cases in Shandong Province. The samples were inoculated into chicken embryo fibroblast and DF-1 cells for virus isolation and identification, respectively. The inoculated cells were screened for three common chicken tumor viruses. Nine strains of avian leukosis virus subgroup J （ALV-J） were identified, and were designated LY1201-LYI209. The env gene from the LY1201 strain was amplified and cloned. All nine resultant env clones （clones 01-09） were sequenced, and the gp85 and gp37 amino acid regions were subjected to homology analysis. Clones 01 and 03 had 10 amino acid deletions in the gp85 region compared to the other seven clones, suggesting that at least two quasispecies with obvious mutations coexist in the same field strain. Among these nine clones, three had identical gp85 and gp37 sequences, and were recognized as the dominant LY1201 quasispecies. The amino acid sequence homology of gp37 and gp85 among the nine clones was 98.5%-100.0% and 96.6%-100.0% respectively, suggesting that the gp85 region of the env gene can better display the quasispecies diversity of ALV-J than gp37.
