<abstract>Aim: To comparatively evaluate the fresh semen quality of 1, 2 and 3-yr-old White Italian ganders (Anser anser L.) and the susceptibility of spermatozoa to freezing-thawing procedure. Methods: Semen wa...<abstract>Aim: To comparatively evaluate the fresh semen quality of 1, 2 and 3-yr-old White Italian ganders (Anser anser L.) and the susceptibility of spermatozoa to freezing-thawing procedure. Methods: Semen was collected by dorso-abdominal massage every 2 days^3 days from three groups of ganders: 1-yr-old (n=11), 2-yr-old (n=7) and 3-yr-old (n=9). In the pooled fresh semen samples, the following parameters were evaluated: the ejaculate volume, the blood or fecal contamination and the motility, concentration and morphology of spermatozoa. Sperm motility and morphology were evaluated in the frozen-thawed semen. Semen diluted with EK extender was frozen in straws in a computerized freezing unit with 6 % dimethyl-formamide to -140 癈 at a rate 60 癈/min and then transferred into the LN2 container. Straws with semen were thawed in a water bath at 60 癈. Results: The ejaculate volume decreased with the age (0.21 mL for 1-yr-old, 0.18 mL for 2-yr-old and 0.14 mL for 3-yr-old ganders); the sperm concentration increased with the age (327xl06mL-1 for 1-yr-old, 431xl06mL-1 for 2-yr-old and 547xl06mL-1 for 3-yr-old ganders); the number of live - normal sperm was significantly (P<0.01) lower in the 1-yr-old than that in the 2- and 3-yr-old ganders (26.61 %, 41.54 %and 35.9 %, respectively). The percentage of normal cells survived the freezing-thawing process was 37.7 %, 43.3 % and 40.9 % for 1-, 2- and 3-yr-old ganders, respectively. Conclusion: Freezing and thawing processes more significantly (P<0.01) affected the motility, viability and morphology of spermatozoa in semen of 1-yr-old ganders in comparison with older males.展开更多
Aim: To evaluate the characteristics of semen produced by one- and two-years old White Italian ganders duringentire reproductive season, in order to clarify whether the young ganders are responsible for a low fertilit...Aim: To evaluate the characteristics of semen produced by one- and two-years old White Italian ganders duringentire reproductive season, in order to clarify whether the young ganders are responsible for a low fertility rate in yougeese. Methods: Males were kept individually in cages under natural light. Semen was collected by dorso-abdonhal massage three times a week and routine examination was performed. Results: The mean ejaculate volume (2and 1.6 mL, respectively) and sperm concentration (323 and 281 × 10~6/mL, respectively) in one-year-old gandwere higher than those of two-year-old ones. The percentages viable spermatozoa of one- and two-year-old gandwere similar (91.4 and 92.3%, respectively), but the percentage of normally formed viable spermatozoa was signicantly higher in the older ganders than in the younger (47.8 and 42.9%, respectively, P < 0.05). Conclusion:The semina from one- or two-year-old Ganders were similar in regard to volume, sperm density and sperm motility,the percentage of normally formed viable spermatozoa, which is critical for fertilization, was significantly higher inolder ganders. It appears that the ganders are responsible for the low fertility rate in young geese.展开更多
文摘<abstract>Aim: To comparatively evaluate the fresh semen quality of 1, 2 and 3-yr-old White Italian ganders (Anser anser L.) and the susceptibility of spermatozoa to freezing-thawing procedure. Methods: Semen was collected by dorso-abdominal massage every 2 days^3 days from three groups of ganders: 1-yr-old (n=11), 2-yr-old (n=7) and 3-yr-old (n=9). In the pooled fresh semen samples, the following parameters were evaluated: the ejaculate volume, the blood or fecal contamination and the motility, concentration and morphology of spermatozoa. Sperm motility and morphology were evaluated in the frozen-thawed semen. Semen diluted with EK extender was frozen in straws in a computerized freezing unit with 6 % dimethyl-formamide to -140 癈 at a rate 60 癈/min and then transferred into the LN2 container. Straws with semen were thawed in a water bath at 60 癈. Results: The ejaculate volume decreased with the age (0.21 mL for 1-yr-old, 0.18 mL for 2-yr-old and 0.14 mL for 3-yr-old ganders); the sperm concentration increased with the age (327xl06mL-1 for 1-yr-old, 431xl06mL-1 for 2-yr-old and 547xl06mL-1 for 3-yr-old ganders); the number of live - normal sperm was significantly (P<0.01) lower in the 1-yr-old than that in the 2- and 3-yr-old ganders (26.61 %, 41.54 %and 35.9 %, respectively). The percentage of normal cells survived the freezing-thawing process was 37.7 %, 43.3 % and 40.9 % for 1-, 2- and 3-yr-old ganders, respectively. Conclusion: Freezing and thawing processes more significantly (P<0.01) affected the motility, viability and morphology of spermatozoa in semen of 1-yr-old ganders in comparison with older males.
文摘Aim: To evaluate the characteristics of semen produced by one- and two-years old White Italian ganders duringentire reproductive season, in order to clarify whether the young ganders are responsible for a low fertility rate in yougeese. Methods: Males were kept individually in cages under natural light. Semen was collected by dorso-abdonhal massage three times a week and routine examination was performed. Results: The mean ejaculate volume (2and 1.6 mL, respectively) and sperm concentration (323 and 281 × 10~6/mL, respectively) in one-year-old gandwere higher than those of two-year-old ones. The percentages viable spermatozoa of one- and two-year-old gandwere similar (91.4 and 92.3%, respectively), but the percentage of normally formed viable spermatozoa was signicantly higher in the older ganders than in the younger (47.8 and 42.9%, respectively, P < 0.05). Conclusion:The semina from one- or two-year-old Ganders were similar in regard to volume, sperm density and sperm motility,the percentage of normally formed viable spermatozoa, which is critical for fertilization, was significantly higher inolder ganders. It appears that the ganders are responsible for the low fertility rate in young geese.
