Sex determination and differentiation play crucial biological roles in sexual reproduction in vertebrates,including zebrafish.Nevertheless,the intricate molecular mechanisms governing these processes have remained eni...Sex determination and differentiation play crucial biological roles in sexual reproduction in vertebrates,including zebrafish.Nevertheless,the intricate molecular mechanisms governing these processes have remained enigmatic.In this study,we showed a pivotal role played by zinc finger GATA-like protein-1(Zglp-1)in sex differentiation in zebrafish.Our findings revealed that homozygous mutants having no Zglp-1 exhibited a female-to-male sex reversal,ultimately resulting in the development of fertile males.Within the pivotal phase of sexual differentiation,zglp-1^(−/−) zebrafish demonstrated a gene expression pattern that was skewed toward a male phenotype.Notably,the expression of amh was upregulated,while the expression of cyp19a1a was not sustained.Furthermore,our data revealed a direct interaction between the zinc fingers of Zglp-1 and Sf-1,which inhibited the ability of Sf-1 to activate the amh promoter.This interaction was crucial for regulating sex differentiation.Moreover,we observed alterations in gonadal cell proliferation and apoptosis in zglp-1^(−/−) zebrafish,which partially contributed to the sexual fate selection.Overall,our findings firmly established Zglp-1 as a crucial regulator of sex differentiation in zebrafish,offering deep insights into the intricate molecular mechanisms that govern sex determination and differentiation in vertebrates.展开更多
To achieve dexterous motion controlling of robot,the sensors that function like human neurons for motion perception are essential.In this work,a silica microfber probe-based optical neuron(MPON)for robot fnger motion ...To achieve dexterous motion controlling of robot,the sensors that function like human neurons for motion perception are essential.In this work,a silica microfber probe-based optical neuron(MPON)for robot fnger motion detection is proposed.The silica microfber probe was fabricated by snapping a biconical silica optical microfber that drawn from the standard optical fbre.Then it was embedded into thin polydimethylsiloxane(PDMS)to detect and recognize motions of robotic fnger.Specifcally,a PDMS-Tefon-Microfber-Tefon-PDMS composite structure was prepared to protect the waveguide structure of silica microfber probe and avoid the environmental pollution.With the help of this composite structure,the proposed MPON achieved the accurate measurement of bending angle with large range and fast response.The repeatability and stability of MPON were also investigated.Additionally,diferent fnger motions were successfully distinguished through observing the output power variation of MPON.The proposed MPON could serve as the perceptron of robot hand,which could be applied in dexterous gesture control even human machine interaction.展开更多
基金supported by the grants of the National Key Research and Development Project of the Ministry of Science and Technology(2023YFE0199500)Shandong Provincial Natural Science Foundation(ZR2022MC032)Science&Tech-nology Innovation Project of Laoshan Laboratory(LSKJ202203204)。
文摘Sex determination and differentiation play crucial biological roles in sexual reproduction in vertebrates,including zebrafish.Nevertheless,the intricate molecular mechanisms governing these processes have remained enigmatic.In this study,we showed a pivotal role played by zinc finger GATA-like protein-1(Zglp-1)in sex differentiation in zebrafish.Our findings revealed that homozygous mutants having no Zglp-1 exhibited a female-to-male sex reversal,ultimately resulting in the development of fertile males.Within the pivotal phase of sexual differentiation,zglp-1^(−/−) zebrafish demonstrated a gene expression pattern that was skewed toward a male phenotype.Notably,the expression of amh was upregulated,while the expression of cyp19a1a was not sustained.Furthermore,our data revealed a direct interaction between the zinc fingers of Zglp-1 and Sf-1,which inhibited the ability of Sf-1 to activate the amh promoter.This interaction was crucial for regulating sex differentiation.Moreover,we observed alterations in gonadal cell proliferation and apoptosis in zglp-1^(−/−) zebrafish,which partially contributed to the sexual fate selection.Overall,our findings firmly established Zglp-1 as a crucial regulator of sex differentiation in zebrafish,offering deep insights into the intricate molecular mechanisms that govern sex determination and differentiation in vertebrates.
基金This work was supported by the National Natural Science Foundation of China(NSFC)(Grant Number:61922033 and 61775072)the Innovation Fund of Wuhan National Laboratory for Optoelectronics(WNLO).
文摘To achieve dexterous motion controlling of robot,the sensors that function like human neurons for motion perception are essential.In this work,a silica microfber probe-based optical neuron(MPON)for robot fnger motion detection is proposed.The silica microfber probe was fabricated by snapping a biconical silica optical microfber that drawn from the standard optical fbre.Then it was embedded into thin polydimethylsiloxane(PDMS)to detect and recognize motions of robotic fnger.Specifcally,a PDMS-Tefon-Microfber-Tefon-PDMS composite structure was prepared to protect the waveguide structure of silica microfber probe and avoid the environmental pollution.With the help of this composite structure,the proposed MPON achieved the accurate measurement of bending angle with large range and fast response.The repeatability and stability of MPON were also investigated.Additionally,diferent fnger motions were successfully distinguished through observing the output power variation of MPON.The proposed MPON could serve as the perceptron of robot hand,which could be applied in dexterous gesture control even human machine interaction.
