The predominant causal agent of poplar leaf blight is the pathogenic fungus Alternaria alternata (Fr.) Keissl., which exhibits host specificity toward Populus species. To elucidate the molecular response mechanisms of...The predominant causal agent of poplar leaf blight is the pathogenic fungus Alternaria alternata (Fr.) Keissl., which exhibits host specificity toward Populus species. To elucidate the molecular response mechanisms of A. alternata under fludioxonil fungicide stress, the fungus was cultured at the half-maximal effective concentration (EC₅₀) of fludioxonil. Transcriptomic and metabolomic profiles were analyzed using mycelia harvested under these conditions. Comparative analysis revealed 1,001 differentially expressed genes (DEGs) in the resistant strain (RS) relative to the wild-type strain (WT), comprising 628 upregulated and 373 downregulated genes. Concurrently, 524 differentially accumulated metabolites (DAMs) were identified, with 336 upregulated and 188 downregulated metabolites. KEGG pathway enrichment demonstrated pronounced upregulation in glycerophospholipid metabolism, α-linolenic acid metabolism, nucleic acid biosynthesis, and glycosylation processes. Conversely, arachidonic acid and galactose metabolism pathways were suppressed. Significant downregulation was observed in phosphatidylinositol signaling, aflatoxin biosynthesis, and cutin/suberin/wax biosynthesis pathways. Transcriptomic profiling further indicated that upregulated DEGs were predominantly associated with amino sugar/nucleotide sugar metabolism, ABC transporters, aflatoxin biosynthesis, and purine metabolism, while downregulated DEGs were enriched in N-glycan biosynthesis, endoplasmic reticulum protein processing, steroid biosynthesis, and riboflavin metabolism. Fludioxonil exerted substantial inhibitory effects on fungal growth, pathogenicity, and metabolic activity. Mechanistically, A. alternata counteracted fungicide-induced stress through modulation of its antioxidant defense system. This integrative multi-omics study delineates the dynamic gene expression and metabolic reprogramming in A. alternata under fludioxonil exposure, providing novel insights into potential molecular targets and informing the development of next-generation fungicidal strategies for phytopathogen control.展开更多
[目的]测定噻呋酰胺与咯菌腈防治水稻纹枯病的最佳复配比例。[方法]采用盆栽法评价噻呋酰胺、咯菌腈及各配比对水稻纹枯病的防效,用孙云沛法评价各配比的增效作用。[结果]噻呋酰胺与咯菌腈1:1和1:4时EC50值分别为13.74、13.9 mg/L,CTC...[目的]测定噻呋酰胺与咯菌腈防治水稻纹枯病的最佳复配比例。[方法]采用盆栽法评价噻呋酰胺、咯菌腈及各配比对水稻纹枯病的防效,用孙云沛法评价各配比的增效作用。[结果]噻呋酰胺与咯菌腈1:1和1:4时EC50值分别为13.74、13.9 mg/L,CTC值分别为163.98和187.67,增效明显。综合考虑药效和成本,选择噻呋酰胺与咯菌腈1:1制成25%噻呋酰胺·咯菌腈SC,田间试验表明,在60 g a.i./hm2剂量下对水稻纹枯病的防效为90.6%,优于对照药剂噻呋酰胺和井冈霉素。[结论]噻呋酰胺与咯菌腈混配对水稻纹枯病有优异的防效,值得推广应用。展开更多
基金supported by the Northeast Asia Biodiversity Research Center(grant number 411147021003).
文摘The predominant causal agent of poplar leaf blight is the pathogenic fungus Alternaria alternata (Fr.) Keissl., which exhibits host specificity toward Populus species. To elucidate the molecular response mechanisms of A. alternata under fludioxonil fungicide stress, the fungus was cultured at the half-maximal effective concentration (EC₅₀) of fludioxonil. Transcriptomic and metabolomic profiles were analyzed using mycelia harvested under these conditions. Comparative analysis revealed 1,001 differentially expressed genes (DEGs) in the resistant strain (RS) relative to the wild-type strain (WT), comprising 628 upregulated and 373 downregulated genes. Concurrently, 524 differentially accumulated metabolites (DAMs) were identified, with 336 upregulated and 188 downregulated metabolites. KEGG pathway enrichment demonstrated pronounced upregulation in glycerophospholipid metabolism, α-linolenic acid metabolism, nucleic acid biosynthesis, and glycosylation processes. Conversely, arachidonic acid and galactose metabolism pathways were suppressed. Significant downregulation was observed in phosphatidylinositol signaling, aflatoxin biosynthesis, and cutin/suberin/wax biosynthesis pathways. Transcriptomic profiling further indicated that upregulated DEGs were predominantly associated with amino sugar/nucleotide sugar metabolism, ABC transporters, aflatoxin biosynthesis, and purine metabolism, while downregulated DEGs were enriched in N-glycan biosynthesis, endoplasmic reticulum protein processing, steroid biosynthesis, and riboflavin metabolism. Fludioxonil exerted substantial inhibitory effects on fungal growth, pathogenicity, and metabolic activity. Mechanistically, A. alternata counteracted fungicide-induced stress through modulation of its antioxidant defense system. This integrative multi-omics study delineates the dynamic gene expression and metabolic reprogramming in A. alternata under fludioxonil exposure, providing novel insights into potential molecular targets and informing the development of next-generation fungicidal strategies for phytopathogen control.
文摘[目的]测定噻呋酰胺与咯菌腈防治水稻纹枯病的最佳复配比例。[方法]采用盆栽法评价噻呋酰胺、咯菌腈及各配比对水稻纹枯病的防效,用孙云沛法评价各配比的增效作用。[结果]噻呋酰胺与咯菌腈1:1和1:4时EC50值分别为13.74、13.9 mg/L,CTC值分别为163.98和187.67,增效明显。综合考虑药效和成本,选择噻呋酰胺与咯菌腈1:1制成25%噻呋酰胺·咯菌腈SC,田间试验表明,在60 g a.i./hm2剂量下对水稻纹枯病的防效为90.6%,优于对照药剂噻呋酰胺和井冈霉素。[结论]噻呋酰胺与咯菌腈混配对水稻纹枯病有优异的防效,值得推广应用。
