Objective:To evaluate the anti-inflammatory and analgesic activities of the ethanol and aqueous extracts of prop roots of Pandanus fascicularis(P.fascicularis) Lam(pandanaceae).And provide experimental evidence for it...Objective:To evaluate the anti-inflammatory and analgesic activities of the ethanol and aqueous extracts of prop roots of Pandanus fascicularis(P.fascicularis) Lam(pandanaceae).And provide experimental evidence for its traditional use such as rheumatoid arthritis and spasmodic. Methods:The anti-inflammatory activity was observed by carrageenan-induced edema of the hind paw of rats.Analgesic activities of prop roots of P.fascicularis were determined using acetic acid induced writhing model and tail clip method in mice and rat,respectively.The ethanol fraction was then subjected to chromatographic analysis and a compound has been isolated and characterized by IR,~1H-NMR and mass spectroscopy.Results:Edema suppressant effect of ethanol extract was found to be 37.03%inhibition whereas aqueous extract was found to be 63.22%inhibition after 3 h which was nearly equivalent to that of 10 mg/kg of indomethacin (67.81%).Percentage inhibition of writhing compared to control were 63.15%,54.38%,14.90%for aspirin,aqueous extract and ethanolic extract,respectively.Both ethanol and aqueous extracts show significant activity against appropriate controls after 60 min of treatment on tail clip method. The structure of the isolated compound is may be characterized as Hepta deca-5-ene-l-ol by analysis it’s IR,H-NMR and mass spectroscopy data.Conclusions:The extracts of prop roots of P.fascicularis produce significant analgesic and anti-inflammatory activities,supporting the traditional application of this herb in treating various diseases associated with inflammation and pain.展开更多
Spermatogonial stem cells(SSCs)have great applications in both reproductive and regenerative medicine.Primates including monkeys are very similar to humans with regard to physiology and pathology.Nevertheless,little i...Spermatogonial stem cells(SSCs)have great applications in both reproductive and regenerative medicine.Primates including monkeys are very similar to humans with regard to physiology and pathology.Nevertheless,little is known about the isolation,the characteristics,and the culture of primate SSCs.This study was designed to identify,isolate,and culture monkey SSCs.Immunocytochemistry was used to identify markers for monkey SSCs.Glial cell line-derived neurotrophic factor family receptor alpha-1(GFRAl)-enriched spermatogonia were isolated from monkeys,namely Macaca fascicularis(M.fascicularis),by two-step enzymatic digestion and magnetic-activated cell sorting,and they were cultured on precoated plates in the conditioned medium.Reverse transcription-polymerase chain reaction(RT-PCR),immunocytochemistry,and RNA sequencing were used to compare phenotype and transcriptomes in GFRAl-enriched spermatogonia between 0 day and 14 days of culture,and xenotransplantation was performed to evaluate the function of GFRAl-enriched spermatogonia.SSCs shared some phenotypes with rodent and human SSCs.GFRAl-enriched spermatogonia with high purity and viability were isolated from M.fascicularis testes.The freshly isolated cells expressed numerous markers for rodent SSCs,and they were cultured for 14 days.The expression of numerous SSC markers was maintained during the cultivation of GFRAl-enriched spermatogonia.RNA sequencing reflected a 97.3%similarity in global gene profiles between 0 day and 14 days of culture.The xenotransplantation assay indicated that the GFRAl-enriched spermatogonia formed colonies and proliferated in vivo in the recipient c-Kitw/w(W)mutant mice.Collectively,GFRAl-enriched spermatogonia are monkey SSCs phenotypically both in vitro and in vivo.This study suggests that monkey might provide an alternative to human SSCs for basic research and application in human diseases.展开更多
Twenty five monkeys were used in this experiment. They were divided into 5 groups with 5 animals as the replicates in each group and were adapted for two weeks to the environment before the data were collected. The an...Twenty five monkeys were used in this experiment. They were divided into 5 groups with 5 animals as the replicates in each group and were adapted for two weeks to the environment before the data were collected. The animals were subjected to 5 experimental diets, i.e. T1 (Basal diet); T2 (Basal diet + palm oil); T3 (Basal diet + palm oil + soybean hull); T4 (Basal diet + cholesterol) and T5 (Basal diet + cholesterol + soybean hull). The diets were given for a period of 8 months and water were given ad lib. Blood serum was taken before and during the experiment. The cholesterol, triglycerides, LDL and HDL were measured using the spectrophotometric method. At the end of the experiment thorax surgery was performed on the animals under general anesthesia. The aorta was removed surgicalIy for histopathological observation stained with hematoxylin and eosine.The results showed that the soybean hull decreased the serum cholesterol level in the groups given palm oil (T2 vs T3) and the groups given cholesterol (T4 vs T5) i.e.: 163.4 vs 124.7 mg/dl and 359 vs 288.5 mg/dl respectively. The soybean hull did not significantly affect the serum triglyceride nor the LDL level when palm oil was given in the diet, but it significantly decreased the two parameters where cholesterol was given in the diet (102.5 vs 98.6 mg/dl triglyceride) and (231 .9 vs 183 mg/dl LDL). The soybean hull did not seem to affect the HDL level.Histopathological observation of the aorta indicated that given T1, T2, T3, T4 and T5 caused 45%, 41 .67%, 31.25%, 86.25% and 53.38% lesion (Atheroma arteriale) resPectively.It was concluded that the soybean hull given in the diet has the ability to prevent the development of atherosclerosis in the aorta of the experimental animals展开更多
Background: This paper describes and displays the spinal radiological anatomy and associated pathology in a Macaca fascicularis and compares it to the spinal anatomy of humans. Animal models are commonly used in resea...Background: This paper describes and displays the spinal radiological anatomy and associated pathology in a Macaca fascicularis and compares it to the spinal anatomy of humans. Animal models are commonly used in research. As compared to Macaca mulatta, the anatomy of M. fascicularis is less well described in the literature. Materials and methods: The authors anatomically reconstructed and reviewed the defleshed spine of a single adult M. fascicularis visually, radiographically, and with high resolution CT. Results: 7 cervical, 12 thoracic, 6 lumbar, 3 sacral, and 16 caudal vertebrae were identified. Similarities in the spine to humans were seen as well as differences such as the beaked anterior arch of C1, the anterior pointed lower lumbar vertebrae, the upward curved transverse processes, and presence of three sacral segments. Degenerative changes were seen at multiple locations similar to humans though most pronounced at T3-4. Conclusions: This paper addresses the normal spinal anatomy and degenerative changes in an adult M. fascicularis and compares it to humans.展开更多
文摘Objective:To evaluate the anti-inflammatory and analgesic activities of the ethanol and aqueous extracts of prop roots of Pandanus fascicularis(P.fascicularis) Lam(pandanaceae).And provide experimental evidence for its traditional use such as rheumatoid arthritis and spasmodic. Methods:The anti-inflammatory activity was observed by carrageenan-induced edema of the hind paw of rats.Analgesic activities of prop roots of P.fascicularis were determined using acetic acid induced writhing model and tail clip method in mice and rat,respectively.The ethanol fraction was then subjected to chromatographic analysis and a compound has been isolated and characterized by IR,~1H-NMR and mass spectroscopy.Results:Edema suppressant effect of ethanol extract was found to be 37.03%inhibition whereas aqueous extract was found to be 63.22%inhibition after 3 h which was nearly equivalent to that of 10 mg/kg of indomethacin (67.81%).Percentage inhibition of writhing compared to control were 63.15%,54.38%,14.90%for aspirin,aqueous extract and ethanolic extract,respectively.Both ethanol and aqueous extracts show significant activity against appropriate controls after 60 min of treatment on tail clip method. The structure of the isolated compound is may be characterized as Hepta deca-5-ene-l-ol by analysis it’s IR,H-NMR and mass spectroscopy data.Conclusions:The extracts of prop roots of P.fascicularis produce significant analgesic and anti-inflammatory activities,supporting the traditional application of this herb in treating various diseases associated with inflammation and pain.
基金the National Natural Science Foundation of China(31671550,31872845)National Key R&D Project(2016YFC1000606)+3 种基金High Level Talent Gathering Project in Hunan Province(2018RS3066)Major Scientific and Technological Projects for Collaborative Prevention and Control of Birth Defect in Hunan Province(2019SK1012)Key Grant of Research and Development in Hunan Province(2020DK2002)The Open Fund of the NHC Key Laboratory of Male Reproduction and Genetics(KF201802).
文摘Spermatogonial stem cells(SSCs)have great applications in both reproductive and regenerative medicine.Primates including monkeys are very similar to humans with regard to physiology and pathology.Nevertheless,little is known about the isolation,the characteristics,and the culture of primate SSCs.This study was designed to identify,isolate,and culture monkey SSCs.Immunocytochemistry was used to identify markers for monkey SSCs.Glial cell line-derived neurotrophic factor family receptor alpha-1(GFRAl)-enriched spermatogonia were isolated from monkeys,namely Macaca fascicularis(M.fascicularis),by two-step enzymatic digestion and magnetic-activated cell sorting,and they were cultured on precoated plates in the conditioned medium.Reverse transcription-polymerase chain reaction(RT-PCR),immunocytochemistry,and RNA sequencing were used to compare phenotype and transcriptomes in GFRAl-enriched spermatogonia between 0 day and 14 days of culture,and xenotransplantation was performed to evaluate the function of GFRAl-enriched spermatogonia.SSCs shared some phenotypes with rodent and human SSCs.GFRAl-enriched spermatogonia with high purity and viability were isolated from M.fascicularis testes.The freshly isolated cells expressed numerous markers for rodent SSCs,and they were cultured for 14 days.The expression of numerous SSC markers was maintained during the cultivation of GFRAl-enriched spermatogonia.RNA sequencing reflected a 97.3%similarity in global gene profiles between 0 day and 14 days of culture.The xenotransplantation assay indicated that the GFRAl-enriched spermatogonia formed colonies and proliferated in vivo in the recipient c-Kitw/w(W)mutant mice.Collectively,GFRAl-enriched spermatogonia are monkey SSCs phenotypically both in vitro and in vivo.This study suggests that monkey might provide an alternative to human SSCs for basic research and application in human diseases.
文摘Twenty five monkeys were used in this experiment. They were divided into 5 groups with 5 animals as the replicates in each group and were adapted for two weeks to the environment before the data were collected. The animals were subjected to 5 experimental diets, i.e. T1 (Basal diet); T2 (Basal diet + palm oil); T3 (Basal diet + palm oil + soybean hull); T4 (Basal diet + cholesterol) and T5 (Basal diet + cholesterol + soybean hull). The diets were given for a period of 8 months and water were given ad lib. Blood serum was taken before and during the experiment. The cholesterol, triglycerides, LDL and HDL were measured using the spectrophotometric method. At the end of the experiment thorax surgery was performed on the animals under general anesthesia. The aorta was removed surgicalIy for histopathological observation stained with hematoxylin and eosine.The results showed that the soybean hull decreased the serum cholesterol level in the groups given palm oil (T2 vs T3) and the groups given cholesterol (T4 vs T5) i.e.: 163.4 vs 124.7 mg/dl and 359 vs 288.5 mg/dl respectively. The soybean hull did not significantly affect the serum triglyceride nor the LDL level when palm oil was given in the diet, but it significantly decreased the two parameters where cholesterol was given in the diet (102.5 vs 98.6 mg/dl triglyceride) and (231 .9 vs 183 mg/dl LDL). The soybean hull did not seem to affect the HDL level.Histopathological observation of the aorta indicated that given T1, T2, T3, T4 and T5 caused 45%, 41 .67%, 31.25%, 86.25% and 53.38% lesion (Atheroma arteriale) resPectively.It was concluded that the soybean hull given in the diet has the ability to prevent the development of atherosclerosis in the aorta of the experimental animals
文摘Background: This paper describes and displays the spinal radiological anatomy and associated pathology in a Macaca fascicularis and compares it to the spinal anatomy of humans. Animal models are commonly used in research. As compared to Macaca mulatta, the anatomy of M. fascicularis is less well described in the literature. Materials and methods: The authors anatomically reconstructed and reviewed the defleshed spine of a single adult M. fascicularis visually, radiographically, and with high resolution CT. Results: 7 cervical, 12 thoracic, 6 lumbar, 3 sacral, and 16 caudal vertebrae were identified. Similarities in the spine to humans were seen as well as differences such as the beaked anterior arch of C1, the anterior pointed lower lumbar vertebrae, the upward curved transverse processes, and presence of three sacral segments. Degenerative changes were seen at multiple locations similar to humans though most pronounced at T3-4. Conclusions: This paper addresses the normal spinal anatomy and degenerative changes in an adult M. fascicularis and compares it to humans.
