The present study explored quality control of plant drug and polyherbal formulation using HPTLC and HPLC fingerprinting.The study combined qualitative analysis of Emodin and Rhein biomarker compound in Rhei Emodi Wall...The present study explored quality control of plant drug and polyherbal formulation using HPTLC and HPLC fingerprinting.The study combined qualitative analysis of Emodin and Rhein biomarker compound in Rhei Emodi Wall.rhizome extract and polyherbal formulation extract by HPTLC method with quantitative estimation of Emodin by HPLC method,aiming to help in accurate identification of Emodin and Rhein in plant materials,polyherbal formulations.This study also checked adulteration,with the purpose of improving bio-efficacy of ayurvedic formulation.All the samples showed characteristic peaks of Emodin at same retention time as that of standard Emodin.According to the HPTLC fingerprinting results,the Rf values of Rhein reference standard at 0.42 and Emodin at 0.62 were observed in test solution of Rhei emodi Wall.rhizome extract and polyherbal formulation extract and both were found comparable.The quantitative evaluation of Emodin present in the polyherbal formulation and Rhei emodi Wall.rhizome extracts were 0.0177% and 0.3106% respectively.展开更多
AIM： To evaluate the antibacterial activity of Sapindus mukorossi （S. mukorossl） and Rheum emodi （R. emodl）. METHODS： Powders of S. mukorossi and R. emodi were extracted successively with petroleum ether, benzen...AIM： To evaluate the antibacterial activity of Sapindus mukorossi （S. mukorossl） and Rheum emodi （R. emodl）. METHODS： Powders of S. mukorossi and R. emodi were extracted successively with petroleum ether, benzene, chloroform and ethanol and were concentrated in vacuum. The disk diffusion method was used for in vitro studies and in vivo studies were performed on male Wister rats. Thirty resistant clinical isolates of H pylon, as determined by their antibiotic sensitivity patterns by E-test, along with two Gram ＋ve （S. aureus, B. subtilis） and two Gram -ve （E. coli, P. vugaris） organisms were screened for their susceptibility patterns against these extracts. RESULTS： In our screening, all 30 resistant isolates and the other four organisms （two Gram ＋ve S. aureus, B. subtilis and two Gram -ve, E. coli, P. vugaris） were sensitive to the test compounds. It was found that ethanol and chloroform extracts of S. mukorossi and ethanol and benzene extracts of R. emodi inhibited Hpylori at very low concentrations, In the in vitro study, the isolates showed a considerable zone of inhibition at very low concentrations （10 μg/mL） for both the extracts. In the in vivo study, the H pylori infection was cleared with minimal doses of extracts of S. mukorossi （2.5 mg/mL） and R. emodi （3.0 mg/mL） given orally for seven days. CONCLUSION： We can conclude from this study that the extracts of S. mukorossi and R. emodi inhibited the growth of pylori in vitro and, in in vivo studies, the H pylori infection cleared within seven days at very low concentrations. We also found that H pylori did not acquire resistance against these herbal extracts even after 10 consecutive passages.展开更多
AIM: To study the hepatoprotective capacity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi) extracts in CCl4 treated male rats. METHODS: The dried powder of S. mukorossi and R. emodi was extracted succ...AIM: To study the hepatoprotective capacity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi) extracts in CCl4 treated male rats. METHODS: The dried powder of S. mukorossi and R. emodi was extracted successively with petroleum ether, benzene, chloroform, and ethanol and concentrated in vacuum. Primary rat hepatocyte monolayer cultures were used for in vitro studies. In vivo, the hepatoprotective capacity of the extract of the fruit pericarp of S. mukorossi and the rhizomes of R. emodi was analyzed in liver injured CCl4-treated male rats. RESULTS: In vitro: primary hepatocytes monolayer cultures were treated with CCl4 and extracts of S. mukorossi & R. emodi. A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : extracts of the fruit pericarp of S. mukorossi (2.5 mg/mL) and rhizomes of R. emodi (3.0 mg/mL) were found to have protective properties in rats with CCl4 induced liver damage as judged from serum marker enzyme activities. CONCLUSION: The extracts of S. mukorossi and R. emodi do have a protective capacity both in vitro on primary hepatocytes cultures and in in vivo in a rat model of CCl4 mediated liver injury.展开更多
A new lignan glycoside was isolated from the n-butanol extract of the roots and rhizomes of Sinopodophyllum emodi.On the basis of spectral evidences,its structure was established and named as 4,5-didemethylpodophyllot...A new lignan glycoside was isolated from the n-butanol extract of the roots and rhizomes of Sinopodophyllum emodi.On the basis of spectral evidences,its structure was established and named as 4,5-didemethylpodophyllotoxin 7＇-O-β-D-glucopyranoside. This compound showed cytotoxicity against four human cancer cell lines（Hela,K562,SH-SY5Y and CNE） in vitro.展开更多
The Garhwal Himalaya is among the major repositories of immensely valuable wild edible plants and provides food security to the local population.Among the valuable plant species that grow in this region,Paeonia emodi(...The Garhwal Himalaya is among the major repositories of immensely valuable wild edible plants and provides food security to the local population.Among the valuable plant species that grow in this region,Paeonia emodi(family Paeoniaceae)is an important wild edible species that found in temperate regions with an altitude range between 1800 and 2800 m.The species is facing a severe threat to its sustainability due to overharvesting,habitat disturbances,and a lack of effort regarding conservation.For the first time,this study investigated anthropogenic pressure,population decline perceptions in the natural habitat,and vulnerability assessment of P.emodi under selected study sites(n=23 villages).A semi structured questionnaire was used to interview approximately 45%of the local inhabitants,including herbal practitioners(Vaidhyas or Dais)of each village.On the basis of demographic characteristics,the perceptions and responses of 464 local people were documented regarding potential causes of deterioration and feasible options for sustainable utilization.Using the weight survey method,we estimated the actual amount of collection based on personal interaction and direct observation.In order to determine the threats status,a rapid vulnerability assessment(RVA)was performed and were used based on the current exploitation and usage.The present study revealed that leaf(100%)was the most frequently harvested part,followed by stem(95.65%),seed(26.09%),root(21.74%)and flower(13.04%).The village Triyuginarayan and Pothivasa recorded the highest collection scores while the purpose of the collection was mostly edible(100%),medicinal(100%),and least commercial(8.70%).According to the literature review and the present survey,the RVA(total=21)is categorized as category II(intermediate side of the RVA index),indicating a degree of vulnerability.The study revealed that P.emodi faces extinction in the Garhwal Himalayas.Growing this species through agro-production techniques may alleviate the pressure on the existing population as a result of the availability of raw materials for commercial and household uses.These findings will provide an effective framework for conservation and management decisions and plans.展开更多
文摘The present study explored quality control of plant drug and polyherbal formulation using HPTLC and HPLC fingerprinting.The study combined qualitative analysis of Emodin and Rhein biomarker compound in Rhei Emodi Wall.rhizome extract and polyherbal formulation extract by HPTLC method with quantitative estimation of Emodin by HPLC method,aiming to help in accurate identification of Emodin and Rhein in plant materials,polyherbal formulations.This study also checked adulteration,with the purpose of improving bio-efficacy of ayurvedic formulation.All the samples showed characteristic peaks of Emodin at same retention time as that of standard Emodin.According to the HPTLC fingerprinting results,the Rf values of Rhein reference standard at 0.42 and Emodin at 0.62 were observed in test solution of Rhei emodi Wall.rhizome extract and polyherbal formulation extract and both were found comparable.The quantitative evaluation of Emodin present in the polyherbal formulation and Rhei emodi Wall.rhizome extracts were 0.0177% and 0.3106% respectively.
文摘AIM： To evaluate the antibacterial activity of Sapindus mukorossi （S. mukorossl） and Rheum emodi （R. emodl）. METHODS： Powders of S. mukorossi and R. emodi were extracted successively with petroleum ether, benzene, chloroform and ethanol and were concentrated in vacuum. The disk diffusion method was used for in vitro studies and in vivo studies were performed on male Wister rats. Thirty resistant clinical isolates of H pylon, as determined by their antibiotic sensitivity patterns by E-test, along with two Gram ＋ve （S. aureus, B. subtilis） and two Gram -ve （E. coli, P. vugaris） organisms were screened for their susceptibility patterns against these extracts. RESULTS： In our screening, all 30 resistant isolates and the other four organisms （two Gram ＋ve S. aureus, B. subtilis and two Gram -ve, E. coli, P. vugaris） were sensitive to the test compounds. It was found that ethanol and chloroform extracts of S. mukorossi and ethanol and benzene extracts of R. emodi inhibited Hpylori at very low concentrations, In the in vitro study, the isolates showed a considerable zone of inhibition at very low concentrations （10 μg/mL） for both the extracts. In the in vivo study, the H pylori infection was cleared with minimal doses of extracts of S. mukorossi （2.5 mg/mL） and R. emodi （3.0 mg/mL） given orally for seven days. CONCLUSION： We can conclude from this study that the extracts of S. mukorossi and R. emodi inhibited the growth of pylori in vitro and, in in vivo studies, the H pylori infection cleared within seven days at very low concentrations. We also found that H pylori did not acquire resistance against these herbal extracts even after 10 consecutive passages.
文摘AIM: To study the hepatoprotective capacity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi) extracts in CCl4 treated male rats. METHODS: The dried powder of S. mukorossi and R. emodi was extracted successively with petroleum ether, benzene, chloroform, and ethanol and concentrated in vacuum. Primary rat hepatocyte monolayer cultures were used for in vitro studies. In vivo, the hepatoprotective capacity of the extract of the fruit pericarp of S. mukorossi and the rhizomes of R. emodi was analyzed in liver injured CCl4-treated male rats. RESULTS: In vitro: primary hepatocytes monolayer cultures were treated with CCl4 and extracts of S. mukorossi & R. emodi. A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : extracts of the fruit pericarp of S. mukorossi (2.5 mg/mL) and rhizomes of R. emodi (3.0 mg/mL) were found to have protective properties in rats with CCl4 induced liver damage as judged from serum marker enzyme activities. CONCLUSION: The extracts of S. mukorossi and R. emodi do have a protective capacity both in vitro on primary hepatocytes cultures and in in vivo in a rat model of CCl4 mediated liver injury.
基金supported by the Chinese National Natural Science Foundation(No.30973869)the Ministry of Science and Technology of China(No.2008BAC39B05)Foundation of Shannxi Provincial Education Department
文摘A new lignan glycoside was isolated from the n-butanol extract of the roots and rhizomes of Sinopodophyllum emodi.On the basis of spectral evidences,its structure was established and named as 4,5-didemethylpodophyllotoxin 7＇-O-β-D-glucopyranoside. This compound showed cytotoxicity against four human cancer cell lines（Hela,K562,SH-SY5Y and CNE） in vitro.
文摘The Garhwal Himalaya is among the major repositories of immensely valuable wild edible plants and provides food security to the local population.Among the valuable plant species that grow in this region,Paeonia emodi(family Paeoniaceae)is an important wild edible species that found in temperate regions with an altitude range between 1800 and 2800 m.The species is facing a severe threat to its sustainability due to overharvesting,habitat disturbances,and a lack of effort regarding conservation.For the first time,this study investigated anthropogenic pressure,population decline perceptions in the natural habitat,and vulnerability assessment of P.emodi under selected study sites(n=23 villages).A semi structured questionnaire was used to interview approximately 45%of the local inhabitants,including herbal practitioners(Vaidhyas or Dais)of each village.On the basis of demographic characteristics,the perceptions and responses of 464 local people were documented regarding potential causes of deterioration and feasible options for sustainable utilization.Using the weight survey method,we estimated the actual amount of collection based on personal interaction and direct observation.In order to determine the threats status,a rapid vulnerability assessment(RVA)was performed and were used based on the current exploitation and usage.The present study revealed that leaf(100%)was the most frequently harvested part,followed by stem(95.65%),seed(26.09%),root(21.74%)and flower(13.04%).The village Triyuginarayan and Pothivasa recorded the highest collection scores while the purpose of the collection was mostly edible(100%),medicinal(100%),and least commercial(8.70%).According to the literature review and the present survey,the RVA(total=21)is categorized as category II(intermediate side of the RVA index),indicating a degree of vulnerability.The study revealed that P.emodi faces extinction in the Garhwal Himalayas.Growing this species through agro-production techniques may alleviate the pressure on the existing population as a result of the availability of raw materials for commercial and household uses.These findings will provide an effective framework for conservation and management decisions and plans.
