摘要
以鬼臼果(Podophyllum emodi)为原料,提取鬼臼果多糖(Podophyllum emodi polysaccharides,PEP),纯化后采用磷酸钠法制备磷酸化修饰鬼臼果多糖(P-PEP),采用单因素实验及响应面法优化了P-PEP的制备工艺。通过高效凝胶渗透色谱、UV吸收光谱、GC-MS、XRD、SEM、TGA、NMR对PEP和P-PEP的理化性质和结构进行了表征,评价了其抗氧化能力和降血糖活性。结果表明,在反应温度72℃、体系pH=9、反应时间5.1 h的最佳工艺条件下,P-PEP的磷酸根取代度为11.37%;PEP和P-PEP的重均相对分子质量分别为20898和21432 Da,是由鼠李糖、阿拉伯糖、半乳糖、葡萄糖4种单糖组成的酸性杂多糖,4种单糖的相对含量显著不同,PEP是以β-糖苷键连接的吡喃糖为主,兼有少量的α-糖苷键连接的吡喃糖,P-PEP是以α-糖苷键连接的吡喃糖为主的多糖。P-PEP比PEP的结晶度降低,呈不规则的小球状和棒状结构形貌,同时热稳定性提高,600℃残炭率从PEP的21.27%提高至48.35%。PEP和P-PEP对1,1-二甲基-2-三硝基苯肼自由基的最大半抑制质量浓度(IC_(50))分别为0.93和0.60 g/L,对2,2'-联氨-二(3-乙基-苯并噻唑-6-磺酸)二铵盐阳离子自由基的IC_(50)分别为1.05和0.67 g/L,对α-葡萄糖苷酶的IC_(50)分别为3.46和1.43 g/L,对α-淀粉酶的IC_(50)分别为3.81和0.49 g/L。P-PEP中鼠李糖(Rha)相对含量(42.31%)比PEP中Rha相对含量(28.04%)显著增大,具有还原性的Rha可以增强多糖的螯合能力,导致P-PEP体外抗氧化活性强于PEP。
Phosphorylated Podophyllum emodi polysaccharides(P-PEP)were prepared from reaction of sodium phosphate with purified Podophyllum emodi polysaccharides(PEP)extracted from Podophyllum emodi fruit,with the preparation process optimized using single-factor experiments and response surface methodology.The physicochemical properties and structures of PEP and P-PEP were characterized by high performance gel permeation chromatography,UV adsorption spectrum,GC-MS,XRD,SEM,TGA and NMR,and their antioxidant capacity and hypoglycemic activity were evaluated.The results showed that the phosphate ion substitution degree of P-PEP was 11.37%under the optimum process conditions of reaction temperature 72℃,pH=9 and reaction time 5.1 h.PEP and P-PEP,with a weight-average relative molecular mass of 20898 and 21432 Da,respectively,were acidic heteropolysaccharides composed of rhamnose,arabinose,galactose and glucose.The relative contents of the four monosaccharides in PEP and P-PEP were significantly different.PEP were polysaccharides mainly composed of pyranose linked byβ-glucoside bonds,with a small amount of pyranose linked byα-glucoside bonds,while P-PEP were polysaccharides mainly composed of pyranose linked byα-glucoside bonds.P-PEP with irregular spheroidal and rod-like structure morphology exhibited lower crystallinity and improved thermal stability compared with PEP,with the carbon residue rate increased from 21.27%to 48.35%at 600℃.The maximum inhibitory mass concentration(IC_(50))of PEP and P-PEP for 1,1-dimethyl-2-trinitrophenylhydrazine free radicals were 0.93 and 0.60 g/L,respectively,and the IC_(50) for 2,2'-hydrazine-bis(3-ethyl-benzothiazole-6-sulfonic acid)diamiammonium cation free radicals were 1.05 and 0.67 g/L,respectively.The IC_(50) forα-glucosidase andα-amylase were 3.46 and 1.43 g/L and 3.81 and 0.49 g/L,respectively.The relative content of rhamnose(Rha)in P-PEP(42.31%)was significantly higher than that in PEP(28.04%),and the reducing Rha could enhance the chelating ability of polysaccharides,resulting in stronger antioxidant activity of P-PEP than PEP in vitro.
作者
王俊龙
江升旗
刘梦瑶
于雪慧
时文盼
蔺永刚
WANG Junlong;JIANG Shengqi;LIU Mengyao;YU Xuehui;SHI Wenpan;LIN Yonggang(Xinjiang Key Laboratory of Clean Conversion and High Value Utilization of Biomass Resources,Yili Normal University,Yili 835000,Xinjiang,China)
出处
《精细化工》
北大核心
2025年第9期1994-2004,共11页
Fine Chemicals
基金
2023年伊犁州应用技术研究与创新驱动专项项目(YYD2023A11)
2024年度伊犁师范大学伊犁河谷重点项目(2024HGZD02)。
关键词
鬼臼果多糖
磷酸化修饰工艺
理化性质
生物活性
中药现代化技术
Podophyllum Emodi polysaccharides
phosphorylation modification process
physicochemical properties
bioactivity
modern technology of Chinese medicine
