Objective:To study the association of environmental adverse factors with early embryo loss,and explore the possible risk factors in daily life. Methods:A questionnaire was administered to 93 new cases of embryo loss(c...Objective:To study the association of environmental adverse factors with early embryo loss,and explore the possible risk factors in daily life. Methods:A questionnaire was administered to 93 new cases of embryo loss(case group) collected in four general hospitals in Tianjin from April 2007 to April 2008 and 93 matched cases of induced abortion(control group) in normal pregnant women who sought the abortion by other reasons.The questionnaire covered information on parental exposure to various environmental factors during and before pregnancy,and the information on daily life.Data were analyzed by single-factor analysis,multiple linear regression and logistic regression analysis.Possible risk factors were identified and odds ratio calculated. Results:Cooking frequently during pregnancy,more daily traffic hours,and decoration history in early pregnancy and paternal exposure to toxic matters three months before pregnancy were associated with early embryo loss, while maternal education was a protective factor. Conclusion:Women exposed to the harmful substances from traffic emissions,cooking and decoration could be at an increased risk of early embryo loss.展开更多
Lymphomas represent one of the most common malignant diseases in young men and an important issue is how treatments will affect their reproductive health.It has been hypothesized that chemotherapies,similarly to envir...Lymphomas represent one of the most common malignant diseases in young men and an important issue is how treatments will affect their reproductive health.It has been hypothesized that chemotherapies,similarly to environmental chemicals,may alter the spermatogenic epigenome.Here,we report the genomic and epigenomic profiling of the sperm DNA from a 31-year-old Hodgkin lymphoma patient who faced recurrent spontaneous miscarriages in his couple 11-26 months after receiving chemotherapy with adriamycin,bleomycin,vinblastine,and dacarbazine(ABVD).In order to capture the potential deleterious impact of the ABVD treatment on mutational and methylation changes,we compared sperm DNA before and 26 months after chemotherapy with whole-genome sequencing(WGS)and reduced representation bisulfite sequencing(RRBS).The WGS analysis identified 403 variants following ABVD treatment,including 28 linked to genes crucial for embryogenesis.However,none were found in coding regions,indicating no impact of chemotherapy on protein function.The RRBS analysis identified 99high-quality differentially methylated regions(hqDMRs)for which methylation status changed upon chemotherapy.Those hqDRMs were associated with 87 differentially methylated genes,among which 14 are known to be important or expressed during embryo development.While no variants were detected in coding regions,promoter regions of several genes potentially important for embryo development contained variants or displayed an altered methylated status.These might in turn modify the corresponding gene expression and thus affect their function during key stages of embryogenesis,leading to potential developmental disorders or miscarriages.展开更多
文摘Objective:To study the association of environmental adverse factors with early embryo loss,and explore the possible risk factors in daily life. Methods:A questionnaire was administered to 93 new cases of embryo loss(case group) collected in four general hospitals in Tianjin from April 2007 to April 2008 and 93 matched cases of induced abortion(control group) in normal pregnant women who sought the abortion by other reasons.The questionnaire covered information on parental exposure to various environmental factors during and before pregnancy,and the information on daily life.Data were analyzed by single-factor analysis,multiple linear regression and logistic regression analysis.Possible risk factors were identified and odds ratio calculated. Results:Cooking frequently during pregnancy,more daily traffic hours,and decoration history in early pregnancy and paternal exposure to toxic matters three months before pregnancy were associated with early embryo loss, while maternal education was a protective factor. Conclusion:Women exposed to the harmful substances from traffic emissions,cooking and decoration could be at an increased risk of early embryo loss.
文摘Lymphomas represent one of the most common malignant diseases in young men and an important issue is how treatments will affect their reproductive health.It has been hypothesized that chemotherapies,similarly to environmental chemicals,may alter the spermatogenic epigenome.Here,we report the genomic and epigenomic profiling of the sperm DNA from a 31-year-old Hodgkin lymphoma patient who faced recurrent spontaneous miscarriages in his couple 11-26 months after receiving chemotherapy with adriamycin,bleomycin,vinblastine,and dacarbazine(ABVD).In order to capture the potential deleterious impact of the ABVD treatment on mutational and methylation changes,we compared sperm DNA before and 26 months after chemotherapy with whole-genome sequencing(WGS)and reduced representation bisulfite sequencing(RRBS).The WGS analysis identified 403 variants following ABVD treatment,including 28 linked to genes crucial for embryogenesis.However,none were found in coding regions,indicating no impact of chemotherapy on protein function.The RRBS analysis identified 99high-quality differentially methylated regions(hqDMRs)for which methylation status changed upon chemotherapy.Those hqDRMs were associated with 87 differentially methylated genes,among which 14 are known to be important or expressed during embryo development.While no variants were detected in coding regions,promoter regions of several genes potentially important for embryo development contained variants or displayed an altered methylated status.These might in turn modify the corresponding gene expression and thus affect their function during key stages of embryogenesis,leading to potential developmental disorders or miscarriages.
