Expression vector p301-bG1 contains a Sw gene and a bialaphos resistance gene both driven by glyceraldehydes-3-phosphate dehydrogenase (GPD) gene promoter isolated from Lentinus edodes ( Berk.) Sing. Using p301-bG1, P...Expression vector p301-bG1 contains a Sw gene and a bialaphos resistance gene both driven by glyceraldehydes-3-phosphate dehydrogenase (GPD) gene promoter isolated from Lentinus edodes ( Berk.) Sing. Using p301-bG1, PEG-mediated transformation of protoplast of L. edodes was studied. Mixed with PEG-purified plasmid DNA, the protoplasts of L. edodes were treated with PEG solution and cultured on CYM regeneration plate containing 40 mug/mL bialaphos. Bialaphos-resistant and GUS-positive transformants were obtained using this transformation system. Although the transformation efficiency was relatively low, the protocols release large expenses on expensive instrument and restriction enzymes, providing a simple and economical method for mushroom breeding at the molecular level.展开更多
The hydroxyl radical（-OH）-scavenging ability of culture filtrates from submerged culture of Lentinula edodes AX3 and five mildews on lignocellulose substance was analysed. Only L. edodes AX3 showed significant -OH-s...The hydroxyl radical（-OH）-scavenging ability of culture filtrates from submerged culture of Lentinula edodes AX3 and five mildews on lignocellulose substance was analysed. Only L. edodes AX3 showed significant -OH-scavenging ability which reached 52.2% at about the 48th hour. All mildews could hardly scavenge -OH under the experimental conditions. -OH-scavenging ability is considered related to the mode and ability of lignocellulose degradation of a strain. The degradation or bioconversion products might be the substance base for.this effect.展开更多
为解析同一品种不同产地香菇的精深加工适宜性,以我国两大香菇主产区湖北随州和浙江庆元的主栽香菇品种‘申香1513’为研究对象,分别采用热水浸提法(hot water extraction)、超声波辅助热水提取法(ultrasonicassisted hot water extract...为解析同一品种不同产地香菇的精深加工适宜性,以我国两大香菇主产区湖北随州和浙江庆元的主栽香菇品种‘申香1513’为研究对象,分别采用热水浸提法(hot water extraction)、超声波辅助热水提取法(ultrasonicassisted hot water extraction)和微波辅助热水提取法(microwave-assisted hot water extraction)三种工艺制备不同水提物(湖北随州:HH、HU和HM;浙江庆元:ZH、ZU和ZM),并利用高效液相色谱法、紫外光谱法和傅里叶红外光谱法等方法,重点比较了其理化性质和结构特征差异。结果显示,6种水提物的多糖含量在37.44%~53.17%,蛋白含量在14.70%~27.03%,HH和ZH的多糖及蛋白含量均高于其余两种提取方法,浙江水提物的蛋白含量均大于湖北水提物,HU和ZM具有相对更高的灰分含量;红外光谱显示6种水提物中存在O-H、CH等多糖特征峰以及N-H等蛋白特征峰;氨基酸分析显示6种水提物的氨基酸种类齐全,且谷氨酸含量最高,其中,ZM的总氨基酸含量最高,HH的总氨基酸含量最低,HU的各氨基酸含量高于HH、HM,ZM的氨基酸含量高于ZH、ZU;分子量分析显示湖北香菇水提物分子量呈现三峰分布,浙江香菇水提物分子量为双峰分布;单糖组成分析表明6种香菇水提物均由甘露糖、盐酸氨基葡萄糖、半乳糖和葡萄糖组成,其中,葡萄糖含量最高,均在90%以上,超声和微波处理均能提高其单糖含量。综上,同一品种不同产地香菇在不同提取工艺下所得水提物理化性质和结构差异显著,若想提取水提物中的有效活性物质,则热水提取法为两地香菇的最佳提取方法,若要得到较高氨基酸含量的水提物,则湖北香菇采用超声辅助热水提取法,浙江香菇采用微波辅助热水提取法,该研究可为不同香菇的精深加工适宜性解析提供模型支撑,亦可为以同一品种、不同产地的香菇水提物作为食品基料,开展新型未来健康食品的精准开发,提供理论依据。展开更多
文摘Expression vector p301-bG1 contains a Sw gene and a bialaphos resistance gene both driven by glyceraldehydes-3-phosphate dehydrogenase (GPD) gene promoter isolated from Lentinus edodes ( Berk.) Sing. Using p301-bG1, PEG-mediated transformation of protoplast of L. edodes was studied. Mixed with PEG-purified plasmid DNA, the protoplasts of L. edodes were treated with PEG solution and cultured on CYM regeneration plate containing 40 mug/mL bialaphos. Bialaphos-resistant and GUS-positive transformants were obtained using this transformation system. Although the transformation efficiency was relatively low, the protocols release large expenses on expensive instrument and restriction enzymes, providing a simple and economical method for mushroom breeding at the molecular level.
文摘The hydroxyl radical（-OH）-scavenging ability of culture filtrates from submerged culture of Lentinula edodes AX3 and five mildews on lignocellulose substance was analysed. Only L. edodes AX3 showed significant -OH-scavenging ability which reached 52.2% at about the 48th hour. All mildews could hardly scavenge -OH under the experimental conditions. -OH-scavenging ability is considered related to the mode and ability of lignocellulose degradation of a strain. The degradation or bioconversion products might be the substance base for.this effect.
文摘为解析同一品种不同产地香菇的精深加工适宜性,以我国两大香菇主产区湖北随州和浙江庆元的主栽香菇品种‘申香1513’为研究对象,分别采用热水浸提法(hot water extraction)、超声波辅助热水提取法(ultrasonicassisted hot water extraction)和微波辅助热水提取法(microwave-assisted hot water extraction)三种工艺制备不同水提物(湖北随州:HH、HU和HM;浙江庆元:ZH、ZU和ZM),并利用高效液相色谱法、紫外光谱法和傅里叶红外光谱法等方法,重点比较了其理化性质和结构特征差异。结果显示,6种水提物的多糖含量在37.44%~53.17%,蛋白含量在14.70%~27.03%,HH和ZH的多糖及蛋白含量均高于其余两种提取方法,浙江水提物的蛋白含量均大于湖北水提物,HU和ZM具有相对更高的灰分含量;红外光谱显示6种水提物中存在O-H、CH等多糖特征峰以及N-H等蛋白特征峰;氨基酸分析显示6种水提物的氨基酸种类齐全,且谷氨酸含量最高,其中,ZM的总氨基酸含量最高,HH的总氨基酸含量最低,HU的各氨基酸含量高于HH、HM,ZM的氨基酸含量高于ZH、ZU;分子量分析显示湖北香菇水提物分子量呈现三峰分布,浙江香菇水提物分子量为双峰分布;单糖组成分析表明6种香菇水提物均由甘露糖、盐酸氨基葡萄糖、半乳糖和葡萄糖组成,其中,葡萄糖含量最高,均在90%以上,超声和微波处理均能提高其单糖含量。综上,同一品种不同产地香菇在不同提取工艺下所得水提物理化性质和结构差异显著,若想提取水提物中的有效活性物质,则热水提取法为两地香菇的最佳提取方法,若要得到较高氨基酸含量的水提物,则湖北香菇采用超声辅助热水提取法,浙江香菇采用微波辅助热水提取法,该研究可为不同香菇的精深加工适宜性解析提供模型支撑,亦可为以同一品种、不同产地的香菇水提物作为食品基料,开展新型未来健康食品的精准开发,提供理论依据。
