摘要
该研究以提取多酚后的香菇剩余物为原料,采用超声辅助水提法提取多糖,以多糖提取得率为评价指标,运用单因素试验结合响应面法优化香菇多糖提取工艺,并探究香菇多糖自由基清除能力,以秀丽隐杆线虫构建体内抗氧化性试验模型,考察香菇多糖对秀丽隐杆线虫体内氧化应激相关指标的影响。结果表明,最佳香菇多糖提取工艺为超声功率240 W、料液比1∶30(g∶m L)、超声时间为50 min、超声温度75℃。在此优化条件下,香菇多糖得率为1.89%。体外抗氧化试验结果显示,香菇多糖对1,1-二苯基-2-三硝基苯肼(DPPH)、2,2'-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)及羟基自由基均表现良好的清除能力,其半抑制浓度(IC50值)分别为1.91 mg/m L、0.58 mg/m L和0.16 mg/m L。体内抗氧化试验结果表明,香菇多糖能够显著提高秀丽隐杆线虫体内超氧歧化酶(SOD)、过氧化氢酶(CAT)的活性(P<0.05),显著抑制丙二醛(MDA)的积累(P<0.05)。因此,香菇多糖具有显著的抗氧化活性。
Taking the residue of Lentinula edodes after polyphenol extraction as the raw material,polysaccharides were extracted by ultrasonic-assisted water extraction method.Using the lentinan yield as the evaluation index,the extraction process of lentinan was optimized by single-factor experiments combined with response surface methodology and the radical scavenging ability of lentinan was investigated.An in vivo antioxidant test model was constructed using Caenorhabditis elegans to investigate the effect of lentinan on oxidative stress-related indicators in C.elegans.The results showed that the optimal extraction conditions for L.edodes polysaccharides were as follows:ultrasonic power 240 W,solid-to-liquid ratio 1∶30(g∶ml),ultrasonic time 50 min,and temperature 75℃.Under these optimal conditions,the yield of lentinan was 1.89%.In vitro antioxidant experiments results demonstrated that lentinan exhibited strong scavenging abilities against 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)and hydroxyl radicals,with half-scavenging concentrations(IC50 value)of 1.91 mg/ml,0.58 mg/ml,and 0.16 mg/ml,respectively.In vivo antioxidant experimental results showed that lentinan significantly enhanced the activities of superoxide dismutase(SOD)and catalase(CAT)(P<0.05),and significantly inhibited the accumulation of malondialdehyde(MDA)(P<0.05)in the body of C.elegans.Therefore,lentinan had significant antioxidant activity.
作者
何小钰
李泉岑
汪鹏翼
李娜
刘斌
曾峰
HE Xiaoyu;LI Quancen;WANG Pengyi;LI Na;LIU Bin;ZENG Feng(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Engineering Research Center of Fujian Subtropical Fruit and Vegetable Processing,Fuzhou 350002,China;National Engineering Research Center of JUNCAO Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《中国酿造》
北大核心
2026年第1期256-262,共7页
China Brewing
基金
江西省重点研发计划项目(20192ACB60008)
福建省科技计划项目(2023N3004)。
关键词
香菇
多糖
提取工艺
响应面优化
秀丽隐杆线虫
抗氧化
Lentinula edodes
polysaccharides
extraction process
response surface optimization
Caenorhabditis elegans
antioxidant
