As climate change,international trade,and human activities increasingly disrupt traditional geographic barriers in the oceans,non-indigenous species(NIS)have successfully established themselves outside their native ra...As climate change,international trade,and human activities increasingly disrupt traditional geographic barriers in the oceans,non-indigenous species(NIS)have successfully established themselves outside their native ranges.Outbreaks of NIS can pose significant threats to local ecosystems and economies,making them a critical issue for marine biodiversity and biosecurity.Biological invasions in marine habitats differ significantly from those on land or in freshwater.Detection and identification of NIS in marine habitats is particularly challenging due to difficulties in sampling,morphological identification,and visualization in the early stages of outbreaks.Environmental DNA(eDNA)approaches have emerged as reliable and cost-effective methods for both qualitative and quantitative detection of marine NIS,particularly in the introductory phase.In this review,we summarize recent applications and advances in eDNA-based detection of marine NIS.We emphasize that innovations in eDNA sampling equipment,improvements in detection methods,and further refinement of the reference genomic database for marine species are crucial for the future development of this field.展开更多
In recent years,environmental DNA(e DNA)has garnered significant attention as a novel tool in biodiversity monitoring,recognized for its efficiency,convenience,and non-invasiveness.Despite its extensive application in...In recent years,environmental DNA(e DNA)has garnered significant attention as a novel tool in biodiversity monitoring,recognized for its efficiency,convenience,and non-invasiveness.Despite its extensive application in various ecological studies,such as conservation,invasion biology,biomonitoring and biodiversity survey assessment,its use in avian monitoring remains in its infancy.This review critically examines the potential and limitations of e DNA technology for avian monitoring,focusing on current advancements and ongoing challenges in this emerging field.Water and air are the primary environmental media for collecting avian e DNA,although other sources like spider webs and plant flowers have been explored as well.Notably,airborne e DNA has been reported to capture the highest diversity of avian species.While avian e DNA technology has shown promise for monitoring rare and endangered species and assessing avian diversity,significant challenges remain,particularly in sampling strategies,DNA extraction methodology,primer selection,and ascertain abundance.Additionally,we discussed the factors influencing the production,transportation,and degradation of avian e DNA in the environment.Finally,we suggested future research directions,including optimizing sampling strategies,developing avian-specific universal primers,expanding avian DNA barcode databases,enhancing e DNA detectability,and integrating environmental RNA(e RNA)and e DNA approaches.展开更多
文摘As climate change,international trade,and human activities increasingly disrupt traditional geographic barriers in the oceans,non-indigenous species(NIS)have successfully established themselves outside their native ranges.Outbreaks of NIS can pose significant threats to local ecosystems and economies,making them a critical issue for marine biodiversity and biosecurity.Biological invasions in marine habitats differ significantly from those on land or in freshwater.Detection and identification of NIS in marine habitats is particularly challenging due to difficulties in sampling,morphological identification,and visualization in the early stages of outbreaks.Environmental DNA(eDNA)approaches have emerged as reliable and cost-effective methods for both qualitative and quantitative detection of marine NIS,particularly in the introductory phase.In this review,we summarize recent applications and advances in eDNA-based detection of marine NIS.We emphasize that innovations in eDNA sampling equipment,improvements in detection methods,and further refinement of the reference genomic database for marine species are crucial for the future development of this field.
基金supported by the Key Research and Development Project of Tianjin,China(23YFZCSN00040)。
文摘In recent years,environmental DNA(e DNA)has garnered significant attention as a novel tool in biodiversity monitoring,recognized for its efficiency,convenience,and non-invasiveness.Despite its extensive application in various ecological studies,such as conservation,invasion biology,biomonitoring and biodiversity survey assessment,its use in avian monitoring remains in its infancy.This review critically examines the potential and limitations of e DNA technology for avian monitoring,focusing on current advancements and ongoing challenges in this emerging field.Water and air are the primary environmental media for collecting avian e DNA,although other sources like spider webs and plant flowers have been explored as well.Notably,airborne e DNA has been reported to capture the highest diversity of avian species.While avian e DNA technology has shown promise for monitoring rare and endangered species and assessing avian diversity,significant challenges remain,particularly in sampling strategies,DNA extraction methodology,primer selection,and ascertain abundance.Additionally,we discussed the factors influencing the production,transportation,and degradation of avian e DNA in the environment.Finally,we suggested future research directions,including optimizing sampling strategies,developing avian-specific universal primers,expanding avian DNA barcode databases,enhancing e DNA detectability,and integrating environmental RNA(e RNA)and e DNA approaches.
