As climate change,international trade,and human activities increasingly disrupt traditional geographic barriers in the oceans,non-indigenous species(NIS)have successfully established themselves outside their native ra...As climate change,international trade,and human activities increasingly disrupt traditional geographic barriers in the oceans,non-indigenous species(NIS)have successfully established themselves outside their native ranges.Outbreaks of NIS can pose significant threats to local ecosystems and economies,making them a critical issue for marine biodiversity and biosecurity.Biological invasions in marine habitats differ significantly from those on land or in freshwater.Detection and identification of NIS in marine habitats is particularly challenging due to difficulties in sampling,morphological identification,and visualization in the early stages of outbreaks.Environmental DNA(eDNA)approaches have emerged as reliable and cost-effective methods for both qualitative and quantitative detection of marine NIS,particularly in the introductory phase.In this review,we summarize recent applications and advances in eDNA-based detection of marine NIS.We emphasize that innovations in eDNA sampling equipment,improvements in detection methods,and further refinement of the reference genomic database for marine species are crucial for the future development of this field.展开更多
In recent years,environmental DNA(e DNA)has garnered significant attention as a novel tool in biodiversity monitoring,recognized for its efficiency,convenience,and non-invasiveness.Despite its extensive application in...In recent years,environmental DNA(e DNA)has garnered significant attention as a novel tool in biodiversity monitoring,recognized for its efficiency,convenience,and non-invasiveness.Despite its extensive application in various ecological studies,such as conservation,invasion biology,biomonitoring and biodiversity survey assessment,its use in avian monitoring remains in its infancy.This review critically examines the potential and limitations of e DNA technology for avian monitoring,focusing on current advancements and ongoing challenges in this emerging field.Water and air are the primary environmental media for collecting avian e DNA,although other sources like spider webs and plant flowers have been explored as well.Notably,airborne e DNA has been reported to capture the highest diversity of avian species.While avian e DNA technology has shown promise for monitoring rare and endangered species and assessing avian diversity,significant challenges remain,particularly in sampling strategies,DNA extraction methodology,primer selection,and ascertain abundance.Additionally,we discussed the factors influencing the production,transportation,and degradation of avian e DNA in the environment.Finally,we suggested future research directions,including optimizing sampling strategies,developing avian-specific universal primers,expanding avian DNA barcode databases,enhancing e DNA detectability,and integrating environmental RNA(e RNA)and e DNA approaches.展开更多
Evaluating species composition and dynamic shifts within fouling communities is essential for developing effective strate-gies to manage biofouling in marine fish aquaculture.The coastal area in the Yellow Sea is a ke...Evaluating species composition and dynamic shifts within fouling communities is essential for developing effective strate-gies to manage biofouling in marine fish aquaculture.The coastal area in the Yellow Sea is a key area for cage aquaculture in China;however,this region faces significant challenges from biofouling organisms.Here,we employed an experimental approach in a filed mesocosm in a net cage aquaculture area in the Yellow Sea,with weekly monitoring of changes in macrofouling species on mesh nets and in the seawater,to assess the utility of water eDNA metabarcoding for identifying macrofoulers.We compared the temporal variation patterns in the composition and diversity of macrofouling communities identified through morphological method as well as COI and 18S rRNA metabarcoding.The results showed that metabarcoding detected the majority of macrofoulers identified through morphological method(64%),and revealed additional species that were overlooked by traditional monitoring approach.Furthermore,the changes in diversity and community composition over sampling dates in COI data were generally consistent with those in morphological identification,although a temporal lag existed between these two approaches.A notable shift in the fouling community occurred at the end of June with the appearance of Ectopleura crocea and Caprella sp.,marking a pivotal change in its structure.Future research could focus on developing targeted primers for these key fouling species,which would enhance the efficiency of monitoring efforts.展开更多
Understanding the seasonal variations of the zooplankton community’s structure in the Western Pacific Warm Pool(WPWP)-the most stable open marine environment in the Pacific Ocean-is crucial to predict the impacts of ...Understanding the seasonal variations of the zooplankton community’s structure in the Western Pacific Warm Pool(WPWP)-the most stable open marine environment in the Pacific Ocean-is crucial to predict the impacts of climate change on the ecosystem.However,knowledge on these variations in this region down to the mesopelagic zone is insufficient.In this study,the environmental DNA(eDNA)metabarcoding method was used to investigate the zooplankton community during summer,autumn,and winter,from the surface to a depth of 1000 m spanning the epipelagic to mesopelagic zones.The zooplankton community structure exhibited seasonal fluctuations at multiple depths except for 200 and 1000 m.In addition,a stronger zooplankton seasonality was particularly recorded in the epipelagic zone than in the mesopelagic zone,which is consistent with the environmental changes.The studied zooplanktons are dominated by medusae and copepods that showed distinct seasonality.At all depths,medusae exhibited greater seasonal variations than the overall zooplankton community,whereas the copepods did not exhibit significant seasonality.The environmental features and the seasons exerted greater influences on the structure of the zooplankton communities than did the spatial factors.The results of this study indicate that eDNA metabarcoding can provide novel insights into zooplankton assemblages due to its ability to capture a rich variety of medusae,which are often underestimated by net collection.展开更多
文摘As climate change,international trade,and human activities increasingly disrupt traditional geographic barriers in the oceans,non-indigenous species(NIS)have successfully established themselves outside their native ranges.Outbreaks of NIS can pose significant threats to local ecosystems and economies,making them a critical issue for marine biodiversity and biosecurity.Biological invasions in marine habitats differ significantly from those on land or in freshwater.Detection and identification of NIS in marine habitats is particularly challenging due to difficulties in sampling,morphological identification,and visualization in the early stages of outbreaks.Environmental DNA(eDNA)approaches have emerged as reliable and cost-effective methods for both qualitative and quantitative detection of marine NIS,particularly in the introductory phase.In this review,we summarize recent applications and advances in eDNA-based detection of marine NIS.We emphasize that innovations in eDNA sampling equipment,improvements in detection methods,and further refinement of the reference genomic database for marine species are crucial for the future development of this field.
基金supported by the Key Research and Development Project of Tianjin,China(23YFZCSN00040)。
文摘In recent years,environmental DNA(e DNA)has garnered significant attention as a novel tool in biodiversity monitoring,recognized for its efficiency,convenience,and non-invasiveness.Despite its extensive application in various ecological studies,such as conservation,invasion biology,biomonitoring and biodiversity survey assessment,its use in avian monitoring remains in its infancy.This review critically examines the potential and limitations of e DNA technology for avian monitoring,focusing on current advancements and ongoing challenges in this emerging field.Water and air are the primary environmental media for collecting avian e DNA,although other sources like spider webs and plant flowers have been explored as well.Notably,airborne e DNA has been reported to capture the highest diversity of avian species.While avian e DNA technology has shown promise for monitoring rare and endangered species and assessing avian diversity,significant challenges remain,particularly in sampling strategies,DNA extraction methodology,primer selection,and ascertain abundance.Additionally,we discussed the factors influencing the production,transportation,and degradation of avian e DNA in the environment.Finally,we suggested future research directions,including optimizing sampling strategies,developing avian-specific universal primers,expanding avian DNA barcode databases,enhancing e DNA detectability,and integrating environmental RNA(e RNA)and e DNA approaches.
基金supported by grants from the National Key Research and Development Program of China(No.2023YFD2401103)the Fundamental Research Funds for the Central Universities.
文摘Evaluating species composition and dynamic shifts within fouling communities is essential for developing effective strate-gies to manage biofouling in marine fish aquaculture.The coastal area in the Yellow Sea is a key area for cage aquaculture in China;however,this region faces significant challenges from biofouling organisms.Here,we employed an experimental approach in a filed mesocosm in a net cage aquaculture area in the Yellow Sea,with weekly monitoring of changes in macrofouling species on mesh nets and in the seawater,to assess the utility of water eDNA metabarcoding for identifying macrofoulers.We compared the temporal variation patterns in the composition and diversity of macrofouling communities identified through morphological method as well as COI and 18S rRNA metabarcoding.The results showed that metabarcoding detected the majority of macrofoulers identified through morphological method(64%),and revealed additional species that were overlooked by traditional monitoring approach.Furthermore,the changes in diversity and community composition over sampling dates in COI data were generally consistent with those in morphological identification,although a temporal lag existed between these two approaches.A notable shift in the fouling community occurred at the end of June with the appearance of Ectopleura crocea and Caprella sp.,marking a pivotal change in its structure.Future research could focus on developing targeted primers for these key fouling species,which would enhance the efficiency of monitoring efforts.
基金Supported by the National Key Research and Development Program of China(No.2022YFC2806805)the National Natural Science Foundation of China(Nos.42076122,42306130)the Digital Deep-sea Typical Habitats Program of China Deep Ocean Affairs Administration(No.DYXZ-02)。
文摘Understanding the seasonal variations of the zooplankton community’s structure in the Western Pacific Warm Pool(WPWP)-the most stable open marine environment in the Pacific Ocean-is crucial to predict the impacts of climate change on the ecosystem.However,knowledge on these variations in this region down to the mesopelagic zone is insufficient.In this study,the environmental DNA(eDNA)metabarcoding method was used to investigate the zooplankton community during summer,autumn,and winter,from the surface to a depth of 1000 m spanning the epipelagic to mesopelagic zones.The zooplankton community structure exhibited seasonal fluctuations at multiple depths except for 200 and 1000 m.In addition,a stronger zooplankton seasonality was particularly recorded in the epipelagic zone than in the mesopelagic zone,which is consistent with the environmental changes.The studied zooplanktons are dominated by medusae and copepods that showed distinct seasonality.At all depths,medusae exhibited greater seasonal variations than the overall zooplankton community,whereas the copepods did not exhibit significant seasonality.The environmental features and the seasons exerted greater influences on the structure of the zooplankton communities than did the spatial factors.The results of this study indicate that eDNA metabarcoding can provide novel insights into zooplankton assemblages due to its ability to capture a rich variety of medusae,which are often underestimated by net collection.
