Surface runoff is mainly generated by two mechanisms, infiltration excess (Horton) runoff and saturation excess (Dunne) runoff; and the spatial variability of soil properties, antecedent soil moisture, topography, and...Surface runoff is mainly generated by two mechanisms, infiltration excess (Horton) runoff and saturation excess (Dunne) runoff; and the spatial variability of soil properties, antecedent soil moisture, topography, and rainfall will result in different surface runoff generation mechanisms. For a large area (e.g., a model grid size of a regional climate model or a general circulation model), these runoff generation mechanisms are commonly present at different portions of a grid cell simultaneously. Missing one of the two major runoff generation mechanisms and failing to consider spatial soil variability can result in significant under/over estimation of surface runoff which can directly introduce large errors in soil moisture states over each model grid cell. Therefore, proper modeling of surface runoff is essential to a reasonable representation of feedbacks in a land-atmosphere system. This paper presents a new surface runoff parameterization with the Philip infiltration formulation that dynamically represents both the Horton and Dunne runoff generation mechanisms within a model grid cell. The parameterization takes into account the effects of soil heterogeneity on Horton and Dunne runoff. The new parameterization is implemented into the current version of the hydrologically based Variable Infiltration Capacity (VIC) land surface model and tested over one watershed in Pennsylvania, USA and over the Shiguanhe Basin in the Huaihe Watershed in China. Results show that the new parameterization plays a very important role in partitioning the water budget between surface runoff and soil moisture in the atmosphere-land coupling system, and has potential applications on large hydrological simulations and land-atmospheric interactions. It is further found that the Horton runoff mechanism should be considered within the context of subgrid-scale spatial variability of soil properties and precipitation.展开更多
邓雅各教授多年来一直是英国德伦大学(the University of Durham)莱特福特教席教授(Lightfoot Professor)、神学系主任,退休后成为名誉系主任。曾担任各种圣经及神学研究协会、委员会、学术期刊编辑等重要职务,多所大学和神学院的客座...邓雅各教授多年来一直是英国德伦大学(the University of Durham)莱特福特教席教授(Lightfoot Professor)、神学系主任,退休后成为名誉系主任。曾担任各种圣经及神学研究协会、委员会、学术期刊编辑等重要职务,多所大学和神学院的客座教授。展开更多
Background Hematopoietic growth factor （HGF） is indispensable to hematopoiesis in the body. The proliferation and differentiation of hematopoietic cells must rely on the existence and stimulation of HGF. This study ...Background Hematopoietic growth factor （HGF） is indispensable to hematopoiesis in the body. The proliferation and differentiation of hematopoietic cells must rely on the existence and stimulation of HGF. This study investigated the effect of catechin, an active component extracted from Spatholobus suberectus Dunn （SSD）, on bioactivity of granulocyte-macrophage colony-stimulating activity （GM-CSA）, burst-promoting activity （BPA） and megakaryocyte colony-stimulating activity （MK-CSA） in spleen condition medium （SPCM） of mice to clarify the hematopoietic mechanism of catechin and SSD. Methods Spleen cells of mice were separated and spleen condition medium （SPCM） was prepared from spleen cell culture. Bone marrow cells of mice were separated and cultured in a culture system including 10% （v/v） SPCM （induced by catechin in vivo or ex vivo） for 6 days. Granulocyte-macrophage colony forming units （CFU-GM）, erythrocyte burst-colony-forming units （BFU-E） and megakaryocyte colony-forming units （CFU-Meg） formation were employed to assay the effects of different treatment on the bioactivity of GM-CSA, BPA and MK-CSA in SPCM. Results SPCM induced by 100 mg/L catechin ex vivo could promote the growth of CFU-GM, BFU-E and CFU-Meg, which indicated that catechin could stimulate the production of GM-CSA, BPA and MK-CSA in SPCM. SPCM prepared at the fourth day of spleen cell culture showed the best stimulating activity. The bioactivity of GM-CSA, BPA and MK-CSA in the SPCM prepared after intraperitoneally injecting catechin into mice was also increased. The number of CFU-GM, BFU-E and CFU-Meg gradually increased as the dose of catechin increased and the time of administration prolonged. CFU-GM, BFU-E and CFU-Meg of the high-dose catechin group were significantly higher than those of the control group （P〈0.01） and reached the maximum at the seventh day after administration. Conclusions This study suggests that catechin extracted from the active acetic ether part of Spatholobus suberectus Dunn can regulate hematopoiesis by inducing bioactivity of GM-CSA, BPA and MK-CSA in SPCM of mice. This may be one of the mechanisms for the hematopoietic-supportive effect of catechin and Spatholobus suberectus Dunn.展开更多
Objective：To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma（HCC） cells in vitro.Methods：Total saponin was extracted from the root of...Objective：To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma（HCC） cells in vitro.Methods：Total saponin was extracted from the root of A.valvata（TSAVD）.HCC cells,such as BEL-7402,HepG2,PLC,SMMC-7721,MHCC-97-H, and MHCC-97-L,were treated with TSAVD in 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenytetrazolium bromide（MTT） assay.BEL-7402 and MHCC-97-H cells were also treated respectively with TSAVD at different concentrations for 24 h in wound healing and adhesion assays,and the effects of TSAVD on BEL-7402 and MHCC-97-H cells mobility and adhesion abilities were observed.Meanwhile,the effects of TSAVD on invasion and migration of BEL-7402 and MHCC-97-H cells were also investigated by transwell chamber in invasion and migration assays. Results：TSAVD at 1.5 mg/mL inhibited BEL-7402 cell proliferation with inhibition ratios（IRs） of 61.08%,74.12%, 84.55%at 24,48,and 72 h,respectively.Meanwhile,TSAVD inhibited MHCC-97-H proliferation in a concentrationdependent manner from 1.5 to 0.5 mg/mL,with the IR of 36%at 1.5 mg/mL at 24 h.For SMMC-7721,PLC, and HepG2,the IR was lower than 30%at 1.5 mg/mL at 24 h.In the wound healing assay,mobility abilities of BEL-7402 and MHCC-97-H cells in TSAVD treated groups were significantly weaker than those of the control group.After pretreatment for 24 h with TSAVD,adhesion abilities were reduced in both MHCC-97-H and BEL-7402 cells,with IRs of 48.50%±4.86%and 49.85%±5.25%at 200 ｜xg/mL.The IRs of MHCC-97-H and BEL-7402 cells in the migration assay were 49.13%±2.91%and 79.37%±0.09%at 200μg/mL In the invasion assay,IRs were 69.78%±4.88%and 82.48%±0.25%at 200μg/mL Conclusions：Of all HCC cells,the highest inhibition by TSAVD was seen for BEL-7402 proliferation.TSAVD could restrain adhesion,invasion,mobility,and migration abilities of BEL-7402 and MHCC-97-H cells in vitro.展开更多
Objective： To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. （A.E.）, a Chinese medicinal herb, against coxsackievirus B3 （CVB3）. Methods： The antiviral effects of A.E. a...Objective： To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. （A.E.）, a Chinese medicinal herb, against coxsackievirus B3 （CVB3）. Methods： The antiviral effects of A.E. against CVB3 in vitro （primarily cultured myocardial cells） and in vivo （BALB/c mice） were determined. Serum pharmacological method was also adopted by in vitro experiments. The effects of A.E. inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo. Results： A.E. exhibited obvious antiviral effects in vivo, and serum samples obtained from the rats with oral administration of A.E. （10 μg/mL, 5 μg/mL） reduced the virus titers in the infected myocardial cells （3.00±0.70, 3.55±0.52, P〈0.01）. Meanwhile, the viral myocarditis induced by CVB3 was inhibited significantly by A.E., and the 15-day mortality was reduced to 40% and 45% （P〈0.01） in mice treated with A.E. at doses of 50 mg/kg and 100 mg/kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively （P〈0.01）. Moreover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E. Conclusion： Aqueous extract of Spatho/obus suberectus Dunn. （A.E.） has inhibitory effect on CVB3 both in vitro and in vivo.展开更多
基金The research reported herein was jointly supported by the National Natural Science Foundation of China under Grant Nos. 40145020, 40275023, 49794030, the National Key Program for Developing Basic Sciences under Grant Nos. G1998040905 and 2001CB309404,
文摘Surface runoff is mainly generated by two mechanisms, infiltration excess (Horton) runoff and saturation excess (Dunne) runoff; and the spatial variability of soil properties, antecedent soil moisture, topography, and rainfall will result in different surface runoff generation mechanisms. For a large area (e.g., a model grid size of a regional climate model or a general circulation model), these runoff generation mechanisms are commonly present at different portions of a grid cell simultaneously. Missing one of the two major runoff generation mechanisms and failing to consider spatial soil variability can result in significant under/over estimation of surface runoff which can directly introduce large errors in soil moisture states over each model grid cell. Therefore, proper modeling of surface runoff is essential to a reasonable representation of feedbacks in a land-atmosphere system. This paper presents a new surface runoff parameterization with the Philip infiltration formulation that dynamically represents both the Horton and Dunne runoff generation mechanisms within a model grid cell. The parameterization takes into account the effects of soil heterogeneity on Horton and Dunne runoff. The new parameterization is implemented into the current version of the hydrologically based Variable Infiltration Capacity (VIC) land surface model and tested over one watershed in Pennsylvania, USA and over the Shiguanhe Basin in the Huaihe Watershed in China. Results show that the new parameterization plays a very important role in partitioning the water budget between surface runoff and soil moisture in the atmosphere-land coupling system, and has potential applications on large hydrological simulations and land-atmospheric interactions. It is further found that the Horton runoff mechanism should be considered within the context of subgrid-scale spatial variability of soil properties and precipitation.
基金This work was supported by a Science Foundation of China （No. grant of the National Natural 30070890）.
文摘Background Hematopoietic growth factor （HGF） is indispensable to hematopoiesis in the body. The proliferation and differentiation of hematopoietic cells must rely on the existence and stimulation of HGF. This study investigated the effect of catechin, an active component extracted from Spatholobus suberectus Dunn （SSD）, on bioactivity of granulocyte-macrophage colony-stimulating activity （GM-CSA）, burst-promoting activity （BPA） and megakaryocyte colony-stimulating activity （MK-CSA） in spleen condition medium （SPCM） of mice to clarify the hematopoietic mechanism of catechin and SSD. Methods Spleen cells of mice were separated and spleen condition medium （SPCM） was prepared from spleen cell culture. Bone marrow cells of mice were separated and cultured in a culture system including 10% （v/v） SPCM （induced by catechin in vivo or ex vivo） for 6 days. Granulocyte-macrophage colony forming units （CFU-GM）, erythrocyte burst-colony-forming units （BFU-E） and megakaryocyte colony-forming units （CFU-Meg） formation were employed to assay the effects of different treatment on the bioactivity of GM-CSA, BPA and MK-CSA in SPCM. Results SPCM induced by 100 mg/L catechin ex vivo could promote the growth of CFU-GM, BFU-E and CFU-Meg, which indicated that catechin could stimulate the production of GM-CSA, BPA and MK-CSA in SPCM. SPCM prepared at the fourth day of spleen cell culture showed the best stimulating activity. The bioactivity of GM-CSA, BPA and MK-CSA in the SPCM prepared after intraperitoneally injecting catechin into mice was also increased. The number of CFU-GM, BFU-E and CFU-Meg gradually increased as the dose of catechin increased and the time of administration prolonged. CFU-GM, BFU-E and CFU-Meg of the high-dose catechin group were significantly higher than those of the control group （P〈0.01） and reached the maximum at the seventh day after administration. Conclusions This study suggests that catechin extracted from the active acetic ether part of Spatholobus suberectus Dunn can regulate hematopoiesis by inducing bioactivity of GM-CSA, BPA and MK-CSA in SPCM of mice. This may be one of the mechanisms for the hematopoietic-supportive effect of catechin and Spatholobus suberectus Dunn.
基金Suppo rted bv the National Natural Science Foundation of China(No C0305020)the Traditional Chinese Medicine Modernization Project of Science and Technology Commission of Shanghai Municipality(No.04DZ19808)the Open Project Foundation of Key Laboratory of Liver and Kidney Diseases(Shanghai University of Traditional Chinese Medicine),Ministry of Education
文摘Objective：To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma（HCC） cells in vitro.Methods：Total saponin was extracted from the root of A.valvata（TSAVD）.HCC cells,such as BEL-7402,HepG2,PLC,SMMC-7721,MHCC-97-H, and MHCC-97-L,were treated with TSAVD in 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenytetrazolium bromide（MTT） assay.BEL-7402 and MHCC-97-H cells were also treated respectively with TSAVD at different concentrations for 24 h in wound healing and adhesion assays,and the effects of TSAVD on BEL-7402 and MHCC-97-H cells mobility and adhesion abilities were observed.Meanwhile,the effects of TSAVD on invasion and migration of BEL-7402 and MHCC-97-H cells were also investigated by transwell chamber in invasion and migration assays. Results：TSAVD at 1.5 mg/mL inhibited BEL-7402 cell proliferation with inhibition ratios（IRs） of 61.08%,74.12%, 84.55%at 24,48,and 72 h,respectively.Meanwhile,TSAVD inhibited MHCC-97-H proliferation in a concentrationdependent manner from 1.5 to 0.5 mg/mL,with the IR of 36%at 1.5 mg/mL at 24 h.For SMMC-7721,PLC, and HepG2,the IR was lower than 30%at 1.5 mg/mL at 24 h.In the wound healing assay,mobility abilities of BEL-7402 and MHCC-97-H cells in TSAVD treated groups were significantly weaker than those of the control group.After pretreatment for 24 h with TSAVD,adhesion abilities were reduced in both MHCC-97-H and BEL-7402 cells,with IRs of 48.50%±4.86%and 49.85%±5.25%at 200 ｜xg/mL.The IRs of MHCC-97-H and BEL-7402 cells in the migration assay were 49.13%±2.91%and 79.37%±0.09%at 200μg/mL In the invasion assay,IRs were 69.78%±4.88%and 82.48%±0.25%at 200μg/mL Conclusions：Of all HCC cells,the highest inhibition by TSAVD was seen for BEL-7402 proliferation.TSAVD could restrain adhesion,invasion,mobility,and migration abilities of BEL-7402 and MHCC-97-H cells in vitro.
基金Supported by the National High Technology Research and Development Program of China(863 Program,No. 2006AA06Z408)
文摘Objective： To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. （A.E.）, a Chinese medicinal herb, against coxsackievirus B3 （CVB3）. Methods： The antiviral effects of A.E. against CVB3 in vitro （primarily cultured myocardial cells） and in vivo （BALB/c mice） were determined. Serum pharmacological method was also adopted by in vitro experiments. The effects of A.E. inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo. Results： A.E. exhibited obvious antiviral effects in vivo, and serum samples obtained from the rats with oral administration of A.E. （10 μg/mL, 5 μg/mL） reduced the virus titers in the infected myocardial cells （3.00±0.70, 3.55±0.52, P〈0.01）. Meanwhile, the viral myocarditis induced by CVB3 was inhibited significantly by A.E., and the 15-day mortality was reduced to 40% and 45% （P〈0.01） in mice treated with A.E. at doses of 50 mg/kg and 100 mg/kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively （P〈0.01）. Moreover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E. Conclusion： Aqueous extract of Spatho/obus suberectus Dunn. （A.E.） has inhibitory effect on CVB3 both in vitro and in vivo.
