By applying the Huber regression algorithm to a relatively new technology of diffuse correlation spectroscopy(DCS),the blood flow index(BFI)from light electric field temporal autocorrelation data is extracted accurate...By applying the Huber regression algorithm to a relatively new technology of diffuse correlation spectroscopy(DCS),the blood flow index(BFI)from light electric field temporal autocorrelation data is extracted accurately via the Nth-order linear(NL)algorithm.The NL algorithm can extract BFI from tissues with irregular geometric shapes,and its accuracy depends on iterative linear regression.The combination of Huber regression with the NL algorithm is proposed in this paper for the first time.The Huber regression is compared with traditional ordinary least square(OLS)regression through computer simulations for evaluation.The results show that the Huber regression is more accurate in extracting BFI than OLS.Compared to the OLS with an error rate of 4.58%,Huber achieves a much smaller error rate(3.54%),indicating its potential in future clinical applications.展开更多
Photon Correlation Spectroscopy is used to study the AOT microemulsion with and without NaCl. Collective diffusion coefficient was investigated by Photon Correlation Spectroscopy technique. We have studied effect of c...Photon Correlation Spectroscopy is used to study the AOT microemulsion with and without NaCl. Collective diffusion coefficient was investigated by Photon Correlation Spectroscopy technique. We have studied effect of charge on dynamic of water-in-oil microemulsion (nano-droplet of water to the oil), which stabilized by AOT and dispersed in n-Decane at water/AOT with 6.7 molar ratio. The small angle X-ray scattering technique and hard sphere model were used to study the structural information of AOT microemulsion with and without NaCl. The structural investigation of samples shows a decrease of length scale of cylindrical droplets with increasing of NaCl concentration in AOT microemulsion.展开更多
We study dynamic and structure of nano-droplets of AOT/water/oil microemulsion with different oils at water/AOT molar ratio of 6.7. Photon correlation spectroscopy is used to study collective diffusion coefficient of ...We study dynamic and structure of nano-droplets of AOT/water/oil microemulsion with different oils at water/AOT molar ratio of 6.7. Photon correlation spectroscopy is used to study collective diffusion coefficient of nano-droplets at AOT microemulsion with decane and cyclohexane. The collective diffusion coefficient of nanodroples is increasing with change of oil from decane to cyclohexane. The structure of AOT microemulsion with decane and cyclohexane is founded with SAXS. Our results show, nano-droplets of AOT microemulsion with decane at X = 6.7 have cylindrical structure and AOT microemulsion with cyclohexane at X = 6.7 have spherical structure.展开更多
Proton magnetic resonance spectroscopy and diffusion tensor imaging are non-invasive techniques used to detect metabolites and water diffusion in vivo. Previous studies have confirmed a positive correlation of individ...Proton magnetic resonance spectroscopy and diffusion tensor imaging are non-invasive techniques used to detect metabolites and water diffusion in vivo. Previous studies have confirmed a positive correlation of individual fractional anisotropy values with N-acetylaspartate/creatine and N-acetylaspartate/choline ratios in tumors, edema, and normal white matter. This study divided the brain parenchyma into tumor, pedtumoral edema, and normal-appearing white matter according to MRI data, and analyzed the correlation of metabolites with water molecular diffusion. Results demonstrated that in normal-appearing white matter, N-acetylaspartate/creatine ratios were positively correlated with fractional anisotropy values, negatively correlated with radial diffusivities, and positively correlated with maximum eigenvalues. Maximum eigenvalues and radial diffusivities in peritumoral edema showed a negative correlation with choline, N-acetylaspartate, and creatine. Radial diffusivities in tumor demonstrated a negative correlation with choline. These data suggest that the relationship between metabolism and structure is markedly changed from normal white matter to peritumoral edema and tumor. Neural metabolism in the peritumoral edema area decreased with expanding extracellular space. The normal relationship of neural function and microstructure disappeared in the tumor region.展开更多
We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Gr...We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Green Fluores-cent Protein (GFP) tagged p53 were incubated with and without DNA damaging agents, cisplatin or eptoposide, which are widely used as chemotherapeutic drugs. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. Together, our results demonstrated that RICS is able to detect the protein dynamics which may be associated with protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening.展开更多
目的初步探讨3 T MRI三维氢质子波谱成像(3D-1H-MRS)、扩散张量成像(DTI)对前列腺癌(PCa)的诊断价值。方法 23例PCa患者、19例良性前列腺增生(BPH)患者及20例健康志愿者均通过3D-1H-MRS及DTI检查,测得PCa、BPH、正常外周带(PZ)、正常中...目的初步探讨3 T MRI三维氢质子波谱成像(3D-1H-MRS)、扩散张量成像(DTI)对前列腺癌(PCa)的诊断价值。方法 23例PCa患者、19例良性前列腺增生(BPH)患者及20例健康志愿者均通过3D-1H-MRS及DTI检查,测得PCa、BPH、正常外周带(PZ)、正常中央区(CG)四组感兴趣区(ROI)内的平均(胆碱+肌酐)/枸橼酸盐(CC/C)值、表观扩散系数(ADC)值、各向异性分数(FA)值并生成相应的参数图,采用单因素方差分析获得四组CC/C、ADC、FA值的差异,并对PCa组的CC/C、ADC、FA值行Pearson相关性分析。结果平均CC/C值由高至低依次为PCa,BPH,CG,PZ,除PZ与CG外,余各组两两之间差异有统计学意义(P<0.05);平均ADC值由高到低依次为BPH,PZ,CG,PCa,除BPH与PZ外,余各组两两之间差异有统计学意义(P<0.05);平均FA值由高到低依次为PCa,CG,BPH,PZ,除PCa与CG外,余各组两两之间差异有统计学意义(P<0.05)。PCa组的CC/C、ADC值呈负相关(r=-0.457,P<0.01),CC/C、FA值呈正相关(r=0.380,P<0.05),ADC、FA值呈负相关(r=-0.400,P<0.05)。结论 PCa、BPH、正常前列腺的CC/C、ADC、FA值存在一定的差异,且PCa 3D-1H-MRS及DTI具有一定的相关性,两者结合对PCa的诊断具有广阔前景。展开更多
基金Shanxi Provincial Key Research and Development Project(No.201903D121149)Natural Science Foundation of Shanxi Province(No.201901D111153)+3 种基金National Natural Science Foundation of China(Nos.61671413,61771433)National Key Scientific Instrument and Equipment Development Project of China(No.2014YQ24044508)OIT Program of Shanxi Province,Shanxi Postgraduate Education Innovation Program(Nos.2020SY366,2020SY367)Graduate Science and Technology Project of North University of China(No.20201726)。
文摘By applying the Huber regression algorithm to a relatively new technology of diffuse correlation spectroscopy(DCS),the blood flow index(BFI)from light electric field temporal autocorrelation data is extracted accurately via the Nth-order linear(NL)algorithm.The NL algorithm can extract BFI from tissues with irregular geometric shapes,and its accuracy depends on iterative linear regression.The combination of Huber regression with the NL algorithm is proposed in this paper for the first time.The Huber regression is compared with traditional ordinary least square(OLS)regression through computer simulations for evaluation.The results show that the Huber regression is more accurate in extracting BFI than OLS.Compared to the OLS with an error rate of 4.58%,Huber achieves a much smaller error rate(3.54%),indicating its potential in future clinical applications.
文摘Photon Correlation Spectroscopy is used to study the AOT microemulsion with and without NaCl. Collective diffusion coefficient was investigated by Photon Correlation Spectroscopy technique. We have studied effect of charge on dynamic of water-in-oil microemulsion (nano-droplet of water to the oil), which stabilized by AOT and dispersed in n-Decane at water/AOT with 6.7 molar ratio. The small angle X-ray scattering technique and hard sphere model were used to study the structural information of AOT microemulsion with and without NaCl. The structural investigation of samples shows a decrease of length scale of cylindrical droplets with increasing of NaCl concentration in AOT microemulsion.
文摘We study dynamic and structure of nano-droplets of AOT/water/oil microemulsion with different oils at water/AOT molar ratio of 6.7. Photon correlation spectroscopy is used to study collective diffusion coefficient of nano-droplets at AOT microemulsion with decane and cyclohexane. The collective diffusion coefficient of nanodroples is increasing with change of oil from decane to cyclohexane. The structure of AOT microemulsion with decane and cyclohexane is founded with SAXS. Our results show, nano-droplets of AOT microemulsion with decane at X = 6.7 have cylindrical structure and AOT microemulsion with cyclohexane at X = 6.7 have spherical structure.
基金supported by the National Natural Science Foundation of China, No. 81171318Shaanxi Provincial Scientific Research Project, No. 2012K13-02-24
文摘Proton magnetic resonance spectroscopy and diffusion tensor imaging are non-invasive techniques used to detect metabolites and water diffusion in vivo. Previous studies have confirmed a positive correlation of individual fractional anisotropy values with N-acetylaspartate/creatine and N-acetylaspartate/choline ratios in tumors, edema, and normal white matter. This study divided the brain parenchyma into tumor, pedtumoral edema, and normal-appearing white matter according to MRI data, and analyzed the correlation of metabolites with water molecular diffusion. Results demonstrated that in normal-appearing white matter, N-acetylaspartate/creatine ratios were positively correlated with fractional anisotropy values, negatively correlated with radial diffusivities, and positively correlated with maximum eigenvalues. Maximum eigenvalues and radial diffusivities in peritumoral edema showed a negative correlation with choline, N-acetylaspartate, and creatine. Radial diffusivities in tumor demonstrated a negative correlation with choline. These data suggest that the relationship between metabolism and structure is markedly changed from normal white matter to peritumoral edema and tumor. Neural metabolism in the peritumoral edema area decreased with expanding extracellular space. The normal relationship of neural function and microstructure disappeared in the tumor region.
文摘We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Green Fluores-cent Protein (GFP) tagged p53 were incubated with and without DNA damaging agents, cisplatin or eptoposide, which are widely used as chemotherapeutic drugs. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. Together, our results demonstrated that RICS is able to detect the protein dynamics which may be associated with protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening.
