期刊文献+
共找到23,952篇文章
< 1 2 250 >
每页显示 20 50 100
黄芪中皂苷类成分UHPLC⁃ELSD分析方法的建立与应用
1
作者 蒋惠 刘伟 +7 位作者 程雪梅 郑立明 毛新民 赵璐 马璇 季志红 李建光 王长虹 《上海中医药杂志》 2026年第1期64-72,81,共10页
目的建立简便、快速、可行的分析黄芪中主要皂苷类成分黄芪皂苷Ⅰ(ASⅠ)、异黄芪皂苷Ⅰ(Iso-ASⅠ)、黄芪皂苷Ⅱ(ASⅡ)、异黄芪皂苷Ⅱ(Iso-ASⅡ)、黄芪皂苷Ⅲ(ASⅢ)、黄芪甲苷(ASⅣ)及环黄芪醇(CA)的含量测定方法,为黄芪质量标准的改进... 目的建立简便、快速、可行的分析黄芪中主要皂苷类成分黄芪皂苷Ⅰ(ASⅠ)、异黄芪皂苷Ⅰ(Iso-ASⅠ)、黄芪皂苷Ⅱ(ASⅡ)、异黄芪皂苷Ⅱ(Iso-ASⅡ)、黄芪皂苷Ⅲ(ASⅢ)、黄芪甲苷(ASⅣ)及环黄芪醇(CA)的含量测定方法,为黄芪质量标准的改进奠定基础。方法通过单因素考察和响应面Box-Behnken设计对样品前处理方法的主要因素提取时间、提取溶剂和料液比进行优化。采用超高效液相色谱仪联用蒸发光散射检测器(UHPLC-ELSD)对黄芪中主要皂苷类成分进行含量测定。色谱条件:Waters HSS T3色谱柱(2.1 mm×100 mm,1.7μm);流动相为A(0.1%甲酸水溶液)-B(乙腈),梯度洗脱程序:0~1 min,28%B~35%B;1~6 min,35%B~35%B;6~13 min,35%B~48%B;13~17 min,48%B~48%B。进样体积为8μL,流速为0.3 mL/min,柱温30℃,漂移管温度为60℃,雾化器温度70℃,气体流速1.6标准升每分钟(SLM)。结果样品前处理优化的最佳条件为加热回流提取2 h,提取溶剂为体积分数70%甲醇溶液,料液比为1∶60。建立的含量测定方法专属性良好,ASⅠ、Iso-ASⅠ、ASⅡ、Iso-ASⅡ、ASⅢ、ASⅣ及CA等7种成分的精密度实验RSD为0.30%~2.89%,加样回收率为95.65%~105.18%;除CA外的其他6个成分的重复性RSD和48 h内样品稳定性RSD分别小于2.92%和2.83%(黄芪样品中CA低于检测限,无重复性和稳定性数据),方法学验证符合要求。含量测定结果表明,黄芪皂苷类化合物之间存在显著正相关关系,且含量与产地和生长年限相关;黄芪皂苷类化合物含量遵循ASⅠ>ASⅡ>Iso-ASⅠ≥ASⅣ>Iso-ASⅡ>ASⅢ的规律,CA水平低于检测限。结论建立了一个简便、快速、高效、分离度好的含量测定方法,实现对多个黄芪皂苷类成分精准、快速测定,为黄芪皂苷类成分质量标志物的选择及科学的黄芪质量标准建立奠定了基础。 展开更多
关键词 黄芪 黄芪皂苷 超高效液相色谱仪联用蒸发光散射检测器 质量控制 中药研究
原文传递
Preparation and measurement of an^(37)Ar source for liquid xenon detector calibration
2
作者 Xu‑Nan Guo Chang Cai +8 位作者 Fei Gao Yang Lei Kai‑Hang Li Chun‑Lei Su Ze‑Peng Wu Xiang Xiao Ling‑Feng Xie Yi‑Fei Zhao Xiao‑Peng Zhou 《Nuclear Science and Techniques》 2026年第1期202-211,共10页
We present the preparation and measurement of the radioactive isotope^(37)Ar,which was produced using thermal neutrons from a reactor,as a calibration source for liquid xenon time projection chambers.^(37)Ar is a low-... We present the preparation and measurement of the radioactive isotope^(37)Ar,which was produced using thermal neutrons from a reactor,as a calibration source for liquid xenon time projection chambers.^(37)Ar is a low-energy calibration source with a half-life of 35.01 days,making it suitable for calibration in the low-energy region of liquid xenon dark-matter experiments.Radioactive isotope^(37)Ar was produced by irradiating ^(36)Ar with thermal neutrons.It was subsequently measured in a gaseous xenon time projection chamber(GXe TPC)to validate its radioactivity.Our results demonstrate that^(37)Ar is an effective and viable calibration source that offers precise calibration capabilities in the low-energy domain of xenon-based detectors. 展开更多
关键词 ^(37)Ar Gaseous xenon detector LOW-ENERGY Calibration source
在线阅读 下载PDF
How Does Urban Public Transit Accessibility Affect Housing Prices?A Comprehensive Analysis with Geographical Detector Combined and Geographically Weighted Regression
3
作者 TANG Jingjing HAN Huiran +3 位作者 YANG Chengfeng XU Lingyi GENG Hui LI Lei 《Chinese Geographical Science》 2026年第1期127-143,共17页
The accessibility of urban public transit directly influences residents’quality of life,travel behavior,and social equity.Its correlation with housing prices has garnered significant attention across disciplines such... The accessibility of urban public transit directly influences residents’quality of life,travel behavior,and social equity.Its correlation with housing prices has garnered significant attention across disciplines such as geography,economics,and urban planning.Although much existing research focuses on the impact of individual transportation facilities on housing prices,there is a notable gap in comprehensive analyses that assess the influence of overall urban transit accessibility on housing market dynamics.This study selected the main urban area of Hefei,China,as a case to investigate the spatial distribution of housing prices and evaluate public transit accessibility in 2022.Employing techniques such as the optimized parameter geographical detector and local spatial regression models,the study aimed to elucidate the effects and underlying mechanisms of urban transit accessibility on housing prices.The findings revealed that:1)housing prices in Hefei exhibited a clustered spatial pattern,with high prices concentrated in the city center and lower prices in peripheral areas,forming three distinct high-price hotspots with a‘belt-like’distribution;2)public transit accessibility showed a‘coreperiphery’structure,with accessibility declining in a‘circumferential’pattern around the city center.Based on the‘housing price-accessibility’dimension,four categories were identified:high price-high accessibility(37.25%),high price-low accessibility(19.07%),low price-high accessibility(21.95%),and low price-low accessibility(21.73%);3)the impact of transit accessibility on housing prices was spatially heterogeneous,with bus travel showing the strongest explanatory power(0.692),followed by automobile,subway,and bicycle travel.The interaction of these transportation modes generated a synergistic effect on housing price differentiation,with most influencing factors contributing more than 25%.These findings offer valuable insights for optimizing the spatial distribution of public transit infrastructure and improving both urban housing quality and residents’living standards. 展开更多
关键词 public transit accessibility housing prices geographically weighted regression geographical detector Hefei City China
在线阅读 下载PDF
Lattice Anchoring Stabilizesα-FAPbI_(3) Perovskite for High-Performance X-Ray Detectors
4
作者 Yu-Hua Huang Su-Yan Zou +5 位作者 Cong-Yi Sheng Yu-Chuang Fang Xu-Dong Wang Wei Wei Wen-Guang Li Dai-Bin Kuang 《Nano-Micro Letters》 2026年第1期337-354,共18页
Formamidinium lead iodide(FAPbI_(3))perovskite exhibits an impressive X-ray absorption coefficient and a large carrier mobility-lifetime product(μτ),making it as a highly promising candidate for X-ray detection appl... Formamidinium lead iodide(FAPbI_(3))perovskite exhibits an impressive X-ray absorption coefficient and a large carrier mobility-lifetime product(μτ),making it as a highly promising candidate for X-ray detection application.However,the presence of larger FA^(+)cation induces to an expansion of the Pb-I octahedral framework,which unfortunately affects both the stability and charge carrier mobility of the corresponding devices.To address this challenge,we develop a novel low-dimensional(HtrzT)PbI_(3) perovskite featuring a conjugated organic cation(1H-1,2,4-Triazole-3-thiol,HtrzT^(+))which matches well with theα-FAPbI_(3) lattices in two-dimensional plane.Benefiting from the matched lattice between(HtrzT)PbI_(3) andα-FAPbI_(3),the anchored lattice enhances the Pb-I bond strength and effectively mitigates the inherent tensile strain of theα-FAPbI_(3) crystal lattice.The X-ray detector based on(HtrzT)PbI_(3)(1.0)/FAPbI_(3) device achieves a remarkable sensitivity up to 1.83×10^(5)μC Gy_(air)^(−1) cm^(−2),along with a low detection limit of 27.6 nGy_(air) s^(−1),attributed to the release of residual stress,and the enhancement in carrier mobility-lifetime product.Furthermore,the detector exhibits outstanding stability under X-ray irradiation with tolerating doses equivalent to nearly 1.17×10^(6) chest imaging doses. 展开更多
关键词 α-FAPbI_(3)perovskite Conjugated organic cation Lattice anchoring Phase stability X-ray detectors
在线阅读 下载PDF
Ultralow dark current soft X-ray detectors based on lead-free double perovskite Cs_(2)AgBiBr_(6)
5
作者 Long Cheng Lijuan Huang +15 位作者 Mulin Sun Ying Meng Yuan Li Tianyu Liu Pengju Tan Mingzhu Hu Huaqing Yang Xiaolan Ma Shunjie Yu Xiaohu Hou Yong Guan Junfa Zhu Xiaosong Liu Yu Li Shibing Long Qin Hu 《Journal of Semiconductors》 2026年第1期109-116,共8页
Soft X-ray detectors play a vital role in materials science,high-energy physics and medical imaging.Cs_(2)AgBiBr_(6),a lead-free double perovskite,has gained attention for its excellent optoelectronic properties,stabi... Soft X-ray detectors play a vital role in materials science,high-energy physics and medical imaging.Cs_(2)AgBiBr_(6),a lead-free double perovskite,has gained attention for its excellent optoelectronic properties,stability,and nontoxicity.However,its fast crystallization and requirement for high-temperature annealing(>250℃)often lead to inferior film quality,limiting its application in flexible devices.This study introduces an alloying strategy that significantly improves the quality of Cs_(2)AgBiBr_(6)thin films annealed at a reduced temperature of 150℃.Devices based on the alloyed thin films exhibit an ultra-low dark current of 0.32 nA·cm^(-2)and a quantum efficiency of 725%.Furthermore,the first successful integration of Cs_(2)AgBiBr_(6)with a thinfilm transistor backplane demonstrates its superior imaging performance,indicating that Cs_(2)AgBiBr_(6)is a promising material for next-generation soft X-ray sensors. 展开更多
关键词 soft X-ray detector lead-free double perovskite Cs_(2)AgBiBr_(6) ALLOYING IMAGING
在线阅读 下载PDF
HPLC-ELSD同时测定地榆升白片中三萜类化学成分的含量 被引量:1
6
作者 吴丽红 嘎力巴 +2 位作者 肖洪彬 杨春娟 王知斌 《化学工程师》 2025年第5期21-23,20,共4页
目的建立HPLC-ELSD同时测定地榆升白片中3种三萜类化合物的含量测定方法。方法以甲醇和0.1%乙酸水溶液为流动相,梯度洗脱,柱温为30℃,流速为0.8mL·min^(-1),雾化管温度为35℃,N_(2)体积流量为1.2L·min^(-1)。结果坡模酸在10.7... 目的建立HPLC-ELSD同时测定地榆升白片中3种三萜类化合物的含量测定方法。方法以甲醇和0.1%乙酸水溶液为流动相,梯度洗脱,柱温为30℃,流速为0.8mL·min^(-1),雾化管温度为35℃,N_(2)体积流量为1.2L·min^(-1)。结果坡模酸在10.7~171.2μg、地榆皂苷Ⅱ在19.2~307.2μg、1β-羟基蔷薇酸在11.4~182.4μg范围内与峰面积线性关系良好。在5批次地榆升白片中,每片坡模酸、地榆皂苷Ⅱ和1β-羟基蔷薇酸的平均含量分别为6.884μg、20.51μg和7.730μg。结论该方法分离效果较好,稳定性较高,可用于地榆升白片的质量控制。 展开更多
关键词 HPLC-elsd 地榆升白片 三萜类化合物 含量测定
暂未订购
HPLC-ELSD法测定“秦药”酸枣仁3种化学成分含量研究 被引量:1
7
作者 李国龙 王磊 +2 位作者 李宝剑 史鑫波 杜升旗 《陕西中医药大学学报》 2025年第3期1-5,共5页
目的建立“秦药”酸枣仁中酸枣仁皂苷A、斯皮诺素和木兰花碱3种化学成分含量测定的高效液相-蒸发光散射检测(HPLC-ELSD)的方法,并检测12批不同产地药材的含量差异。方法采用SunFire C_(18)柱(250 mm×4.6 mm,5μm);乙腈-水进行梯度... 目的建立“秦药”酸枣仁中酸枣仁皂苷A、斯皮诺素和木兰花碱3种化学成分含量测定的高效液相-蒸发光散射检测(HPLC-ELSD)的方法,并检测12批不同产地药材的含量差异。方法采用SunFire C_(18)柱(250 mm×4.6 mm,5μm);乙腈-水进行梯度洗脱;流速1.0 mL·min^(-1);柱温30℃;紫外检测波长为227 nm和335 nm,蒸发光散射检测器(ELSD)设置温度105℃,空气流量为1.6 mL·min^(-1)。结果3种化学成分得到较好的分离,3种化学成分浓度与峰面积成良好的线性关系,相关系数0.9993~0.9997,回收率在97.83%~102.41%,RSD值均小于5%。不同产地的“秦药”酸枣仁3种化学成分含量差异较小。结论该方法快速方便、成本低,可用于同时测定酸枣仁中3种化学成分的含量,可为“秦药”酸枣仁资源应用和开发提供参考。 展开更多
关键词 秦药 酸枣仁 HPLC-elsd 不同产地 含量研究
暂未订购
Determination of AF and AFG in Red Ginseng by High Performance Liquid Chromatography with Evaporative Light Scattering Detector (HPLC-ELSD) 被引量:2
8
作者 Chen Wei He Da Shao Ying 《Animal Husbandry and Feed Science》 CAS 2019年第5期131-134,共4页
[Objective]The paper was to establish a method for determining AF and AFG in red ginseng.[Method]A new simple,rapid and sensitive method for simultaneous determination of two amadori compounds,arginyl-fructose(AF)and ... [Objective]The paper was to establish a method for determining AF and AFG in red ginseng.[Method]A new simple,rapid and sensitive method for simultaneous determination of two amadori compounds,arginyl-fructose(AF)and arginyl-fructosyl-glucose(AFG),in extracts of three kinds of ginseng preparations was developed and validated using high performance liquid chromatography with evaporative light scattering detector(HPLC-ELSD).Two target analytes were efficiently separated by Prevail CTM18 column(4.6 mm×250 mm,5μm)at the flow rate of 0.8 mL/min within 15 min of single chromatographic run.[Result]Under optimized conditions,the detection limits were 0.015 and 0.02 mg/mL for AF and AFG,respectively.Calibration curves of peak area for two analytes were linear over three orders of magnitude with the correlation coefficients greater than 0.999.The average recoveries,precision,reproducibility and stability for two analytes(AF and AFG)were 99.5% and 100.9%,0.43% and 0.47%,0.46% and 0.43%,0.41% and 0.49%,respectively.[Conclusion]This method was successfully applied for quantifying AF and AFG in red ginseng and the method was efficient,sensitive and accurate. 展开更多
关键词 High performance liquid chromatography Evaporative light scattering detector Amadori compounds Red ginseng
在线阅读 下载PDF
HPLC-ELSD法测定银杏达莫注射液中吐温-80的含量 被引量:1
9
作者 田甜 王璐 +1 位作者 祝晶 漆欣筑 《山东化工》 2025年第2期213-215,共3页
目的:建立高效液相色谱-蒸发光散射检测法测定银杏达莫注射液中吐温-80的含量。方法:采用HPLC-ELSD法,G2000SWXL凝胶色谱柱,柱温为30℃;以乙腈-0.02 mol/L醋酸铵(体积比10∶90)为流动相;漂移管温度:103℃;氮气流速2.3 mL/min。结果:吐... 目的:建立高效液相色谱-蒸发光散射检测法测定银杏达莫注射液中吐温-80的含量。方法:采用HPLC-ELSD法,G2000SWXL凝胶色谱柱,柱温为30℃;以乙腈-0.02 mol/L醋酸铵(体积比10∶90)为流动相;漂移管温度:103℃;氮气流速2.3 mL/min。结果:吐温80在111.1~2221.6μg/mL的范围内呈良好的线性关系(Y=1.4582X+2.3569,r=0.9996);方法重复性良好(RSD=0.2%);方法回收率为103.1%,RSD为1.5%(n=9);样品10 h内稳定性良好(RSD=0.6%)。结论:该方法用于测定银杏达莫注射液中吐温80的含量,专属性强,准确度较高,样品稳定性良好,为注射剂中吐温-80的含量测定提供参考,对保证注射剂的安全性具有实际意义。 展开更多
关键词 HPLC-elsd 银杏达莫注射液 吐温-80 含量测定
在线阅读 下载PDF
HPLC-ELSD法测定多种来源玄参中单糖和低聚糖的含量
10
作者 宋舒雨 张米娜 +1 位作者 张刘强 李医明 《中药新药与临床药理》 北大核心 2025年第8期1327-1335,共9页
目的建立玄参中单糖(果糖、葡萄糖)和低聚糖(蔗糖、棉子糖和水苏糖)的一测多评含量测定方法,并进行综合质量评价。方法采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定不同产地、不同加工方法来源玄参及市售玄参饮片中单糖(果糖、... 目的建立玄参中单糖(果糖、葡萄糖)和低聚糖(蔗糖、棉子糖和水苏糖)的一测多评含量测定方法,并进行综合质量评价。方法采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定不同产地、不同加工方法来源玄参及市售玄参饮片中单糖(果糖、葡萄糖)和低聚糖(蔗糖、棉子糖和水苏糖)的含量,并采用聚类分析、主成分分析综合评价不同产地、不同加工方法来源玄参及市售玄参饮片的质量差异。结果2种单糖和3种低聚糖分离度良好。果糖、葡萄糖、蔗糖、棉子糖和水苏糖在25.43~508.50、55.60~556.00、57.00~570.00、43.00~430.00、156.65~3133.00μg·mL^(-1)浓度范围内线性关系良好(r≥0.9990);平均加样回收率为96.43%~101.29%,RSD为1.02%~2.90%。果糖、葡萄糖、蔗糖、棉子糖和水苏糖在38批不同玄参样品中的含量分别为8.05~52.32、6.76~81.15、12.36~104.69、11.13~117.18、269.38~869.12 mg·g^(-1)。除了产自贵州道真的冻干玄参样品(S5)外,其他产地的玄参样品中水苏糖含量均高于60%。聚类分析和主成分分析结果显示,38批不同玄参样品主要分为两类,大部分冻干(FDM)玄参样品和短时(<12 h)蒸制(TPM1)样品为一类;发汗(WPM)玄参样品和长时间(≥12 h)蒸制(TPM2)样品聚为一类。结论该研究建立的方法简便快速,重复性好,灵敏度高,易于操作,可为优化玄参炮制工艺参数、推进产地加工一体化生产提供科学依据。 展开更多
关键词 玄参 单糖 低聚糖 高效液相色谱 蒸发光散射检测器 含量测定
原文传递
HPLC-ELSD测定自来水中HEDP的含量 被引量:1
11
作者 张振永 杨晓丹 +2 位作者 张辰元 康健 高婉婧 《化学研究与应用》 北大核心 2025年第2期470-474,共5页
开发了一种用于自来水中羟基乙叉二膦酸(1-hydroxyethylidene-1,1-diphosphonic acid,HEDP)含量测定的液相色谱方法。采用Shim-pack Scepter Diol-HILIC-120色谱柱分析,蒸发光散射检测器(evaporative light scattering detector,ELSD)... 开发了一种用于自来水中羟基乙叉二膦酸(1-hydroxyethylidene-1,1-diphosphonic acid,HEDP)含量测定的液相色谱方法。采用Shim-pack Scepter Diol-HILIC-120色谱柱分析,蒸发光散射检测器(evaporative light scattering detector,ELSD)进行检测。水相采用0.2 mol·L^(-1)的甲酸铵(pH 3.0),有机相采用乙腈,梯度洗脱。在优化条件下,HEDP峰形良好,可在16 min内完成测定,且和自来水中的干扰离子完全分离。在10.0~500.0 mg·L^(-1)范围内线性关系良好,线性相关系数大于0.995。HEDP的检出限为2.20 mg·L^(-1),定量限为7.35 mg·L^(-1)。HEDP在3个不同水平的加标回收率和相对标准偏差(RSDs)范围分别为87.1%~105%和1.4%~4.4%。该方法可为自来水中HEDP的含量测定提供参考。 展开更多
关键词 液相色谱法 蒸发光散射检测器 HEDP 自来水
在线阅读 下载PDF
HPLC-ELSD法同时测定党参休眠过程中5种可溶性糖含量 被引量:2
12
作者 唐顺莉 张延红 +3 位作者 杜弢 陈晖 何春雨 董婉琦 《作物杂志》 北大核心 2025年第1期202-207,共6页
建立高效液相色谱―蒸发光散射(HPLC-ELSD)法同时测定党参5种可溶性糖含量的技术方法,探明可溶性糖在党参抗寒及休眠过程中发挥的作用。5种可溶性糖适宜的检测条件为Analysis Column(4.6 mm×250 mm 5-Micron)色谱柱,流动相为乙腈:... 建立高效液相色谱―蒸发光散射(HPLC-ELSD)法同时测定党参5种可溶性糖含量的技术方法,探明可溶性糖在党参抗寒及休眠过程中发挥的作用。5种可溶性糖适宜的检测条件为Analysis Column(4.6 mm×250 mm 5-Micron)色谱柱,流动相为乙腈:水(70:30)溶液,柱温35℃,流速1.0 m L/min;ELSD参数:雾化温度50℃,漂移管温度110℃,氮气流速2.2 m L/min,增益值1.0。在党参自然休眠过程中海藻糖只在休眠前期的根和休眠期的芽中检测到;棉子糖只在休眠前期的根中检测到;果糖和葡萄糖在休眠前期的叶中含量较高,蔗糖在休眠前期的根和休眠期的芽中含量较高,三者均在解除休眠后的芽中含量最高。本研究建立了高效的HPLC-ELSD法同时检测5种可溶性糖的技术体系,明确了海藻糖和棉子糖是党参抗寒的主要可溶性糖组分,其中海藻糖在根中合成并进一步转运至芽中。果糖、葡萄糖和蔗糖是党参根中储存的可溶性糖组分,也是萌发的物质和能量来源。 展开更多
关键词 党参 可溶性糖组分 高效液相色谱―蒸发光散射法 休眠 逆境生理
原文传递
RAA-DETECTOR布鲁氏菌核酸快速检测方法的建立及应用
13
作者 张雲龙 王靖雷 +8 位作者 朱亚杰 张明洁 康澳 周翔 魏凯 曹洪防 李强 王勇 苏峰 《畜牧兽医学报》 北大核心 2025年第10期5115-5124,共10页
本研究旨在建立不依赖于专业设备的布鲁氏菌病高效快速检测体系,实现临床样本40 min内快速检测。首先比对不同布鲁氏菌的基因组,找到布鲁氏菌的核心共有序列,以布鲁氏菌S2菌株基因SEQ NO.6(CN 105018489A)为模板构建阳性质粒,用于后续试... 本研究旨在建立不依赖于专业设备的布鲁氏菌病高效快速检测体系,实现临床样本40 min内快速检测。首先比对不同布鲁氏菌的基因组,找到布鲁氏菌的核心共有序列,以布鲁氏菌S2菌株基因SEQ NO.6(CN 105018489A)为模板构建阳性质粒,用于后续试验;通过原核表达的方法分别表达CRISPR/AsCas12a和LwaCas13a蛋白;通过重组酶介导的等温核酸扩增技术(RAA)、体外表达技术结合AsCas12a/LwaCas13a的DETECTOR系统,优化高效检测体系、通过无限稀释法等进行检测。结果发现,设计的RAA引物能够有效扩增样本中的DNA片段,DETECTOR得到最佳反应的AsCas12a浓度为200 nmol·L^(-1),gRNA为200 nmol·L^(-1);Cas13a浓度为300 nmol·L^(-1),crRNA浓度为400 nmol·L^(-1);RAA-AsCas12a和RAA-LwaCas13a的检测体系的最佳反应时间为35 min,侧平流式纸条的最佳检测时间为20 min,细菌最低检测浓度为10 Copies·μL^(-1),且具有较好的检测特异性,临床样本的检测试验表明两种方法均有较好的重复性和检测精度,但LwaCas13a的精测精度更高,但二者无显著差异。该研究建立了2种RAA-CRISPR/Cas布鲁氏菌核酸快速检测体系,在临床上可获得较好的应用,RAA-LwaCas13a的检测方法精度更高。 展开更多
关键词 布鲁氏菌 RAA detector系统 SHERLOCK系统 AsCas12a LwaLwaCas13a
在线阅读 下载PDF
CMOS direct conversion X-ray detector coupled with fluorinated liquid 被引量:1
14
作者 Shi-Hua Liu Chao-Song Gao +5 位作者 Xin Zhang Xiang-Ming Sun Meng Wu Zhi-Hui Han Tong Wan Yong-Shuai Ge 《Nuclear Science and Techniques》 2025年第1期59-68,共10页
X-ray detectors show potential applications in medical imaging,materials science,and nuclear energy.To achieve high detection efficiency and spatial resolution,many conventional semiconductor materials,such as amorpho... X-ray detectors show potential applications in medical imaging,materials science,and nuclear energy.To achieve high detection efficiency and spatial resolution,many conventional semiconductor materials,such as amorphous selenium,cadmium telluride zinc,and perovskites,have been utilized in direct conversion X-ray detectors.However,these semiconductor materials are susceptible to temperature-induced performance degradation,crystallization,delamination,uneven lattice growth,radiation damage,and high dark current.This study explores a new approach by coupling an FC40 electronic fluorinated liquid with a specialized high-resolution and high-readout-speed complementary metal-oxide-semiconductor(CMOS)pixel array,specifically the Topmetal II−chip,to fabricate a direct conversion X-ray detector.The fluorinated liquid FC40(molecular formula:C_(21)F_(48)N_(2))is an electronic medium that is minimally affected by temperature and displays no issues with uniform conductivity.It exhibits a low dark current and minimal radiation damage and enables customizable thickness in X-ray absorption.This addresses the limitations inherent in conventional semiconductor-based detectors.In this study,simple X-ray detector imaging tests were conducted,demonstrating the excellent coupling capability between FC40 electronic fluorinated liquid and CMOS chips by the X-ray detector.A spatial resolution of 4.0 lp/mm was measured using a striped line par card,and a relatively clear image of a cockroach was displayed in the digital radiography imaging results.Preliminary test results indicated the feasibility of fabricating an X-ray detector by combining FC40 electronic fluorinated liquid and CMOS chips.Owing to the absence of issues related to chip-material coupling,a high spatial resolution could be achieved by reducing the chip pixel size.This method presents a new avenue for studies on novel liquid-based direct conversion X-ray detectors. 展开更多
关键词 FC40 electronic fluorinated liquid CMOS pixel chip X-ray detector Spatial resolution
在线阅读 下载PDF
Optimizing neon-based gas mixtures for two-stage amplification fast-timing Micromegas detectors
15
作者 Yue Meng Xu Wang +3 位作者 Jianbei Liu Ming Shao Zhiyong Zhang Yi Zhou 《中国科学技术大学学报》 北大核心 2025年第4期59-66,58,I0002,共10页
The PICOSEC Micromegas(MM)is a precise timing gaseous detector based on a Cherenkov radiator coupled with a semi-transparent photocathode and an MM amplifying structure.It features a two-stage amplification process th... The PICOSEC Micromegas(MM)is a precise timing gaseous detector based on a Cherenkov radiator coupled with a semi-transparent photocathode and an MM amplifying structure.It features a two-stage amplification process that leads to a significant deterioration of non-uniformity when scaling up to larger areas.Since the performance of gaseous detectors is highly dependent on the choice of working gas,optimizing the gas mixture offers a promising solution to improve the uniformity performance.This paper addresses these challenges through a combined approach of simulation based on Garfield++and experimental studies.The simulation investigates the properties of different mixing fractions of gas mixtures and their impact on detector performance,including gain uniformity and time resolution.To verify the simulation results,experimental tests were conducted using a multi-channel PICOSEC MM prototype with different gas mixtures.The experimental results are consistent with the findings of the simulation,indicating that a higher concentration of neon significantly improves the detector’s gain uniformity.Furthermore,the influence of gas mixtures on time resolution was explored as a critical performance indicator.The study presented in this paper offers valuable insights for improving uniformity in large-area PICOSEC MM detectors and optimizing overall performance. 展开更多
关键词 gaseous detectors MICROMEGAS timing detectors fast-timing Micromegas Monte Carlo simulation gain uniformity
在线阅读 下载PDF
A prototype of 2μm balanced detector for space-borne Coherent Doppler Wind Lidar
16
作者 CHEN Bao-Lin JIN Ge +2 位作者 WANG Chong CHEN Lian 《红外与毫米波学报》 北大核心 2025年第6期1021-1029,共9页
Despite rapid advancements in lidar technology,extremely long-range observation remains a signifi⁃cant challenge.Recently,2μm lasers have demonstrated a potential to be applied in CDWL(Coherent Doppler Wind Lidar)sys... Despite rapid advancements in lidar technology,extremely long-range observation remains a signifi⁃cant challenge.Recently,2μm lasers have demonstrated a potential to be applied in CDWL(Coherent Doppler Wind Lidar)system,for its high atmospheric penetration capability through the atmosphere and high potential la⁃ser power.In this study,we present a 2μm balanced detector that consists of a pair of commercial positive-intrin⁃sic-negative(PIN)diodes with a low-noise transimpedance circuit.To meet the high bandwidth requirements,the highspeed transimpedance circuit and bias voltage tuning method were utilized to overcome the large capaci⁃tance of PIN diodes.The circuit transfer function,stability analysis and noise calculation have been studied.The detector was co-packaged with a data acquisition module for convenient data transmission and bias voltage con⁃trol.The characteristics of the detector,including bandwidth,noise and bias voltage influence,are evaluated in laboratory.Results show that the RMS value of the balanced detector background noise is 539μV and the band⁃widths of the two diodes are 110.8 MHz and 110.3 MHz,respectively.The evaluation results show that the bal⁃anced detector meets the wind measurement requirements and allows for a 1.45×increase in bandwidth through bi⁃as voltage tuning.Our work offers insights into lidar detector design and bandwidth enhancement,providing a valuable reference for researchers and professionals in the field.More importantly,it lays a critical foundation for fu⁃ture ultra-long-range and space-borne 2μm coherent wind lidar systems by addressing key device-level challenges. 展开更多
关键词 balanced detector transimpedance circuit CDWL laser detection
在线阅读 下载PDF
Study of bonding layer for integrated structure of space gravitational wave detector telescope
17
作者 ZHAO Hong-chao LIU Chang +2 位作者 ZHOU Wen-ke ZHU Han-bin CHEN Wen-duo 《中国光学(中英文)》 北大核心 2025年第3期715-724,共10页
To detect space gravitational waves in the extremely low-frequency band,the telescope and optic-al platform require high stability and reliability.However,the cantilevered design presents challenges,espe-cially in the... To detect space gravitational waves in the extremely low-frequency band,the telescope and optic-al platform require high stability and reliability.However,the cantilevered design presents challenges,espe-cially in the glass-metal hetero-bonding process.This study focuses on the analysis and experimental re-search of the bonding layer in the integrated structure.By optimizing the structural configuration and select-ing suitable bonding processes,the reliability of the telescope system is enhanced.The research indicates that using J-133 adhesive achieves the best performance,with a bonding layer thickness of 0.30 mm and a metal substrate surface roughness of Ra 0.8.These findings significantly enhance the reliability of the optical sys-tem while minimizing potential risks. 展开更多
关键词 space gravitational-wave detector integrated structure glass-metal hetero-bonding
在线阅读 下载PDF
Unbiased test of the hyperon CP violation in terms of detector resolution at STCF
18
作者 Ming Xu Liang Liu +2 位作者 Yupeng Pei Xiaorong Zhou Yingchun Zhu 《中国科学技术大学学报》 北大核心 2025年第4期41-47,40,I0002,共9页
The study of the charge conjugation and parity(CP)violation of hyperon is the precision frontier for probing possible new CP violation sources beyond the standard model(SM).With the large number of quantum entangled h... The study of the charge conjugation and parity(CP)violation of hyperon is the precision frontier for probing possible new CP violation sources beyond the standard model(SM).With the large number of quantum entangled hyperonantihyperon pairs to be produced at Super Tau-Charm Facility(STCF),the CP asymmetry of hyperon is expected to be tested with a statistical sensitivity of 10^(−4) or even better.To cope with the statistical precision,the systematic effects from various aspects are critical and need to be studied in detail.In this paper,the sensitivity effects on the CP violation parameters associated with the detector resolution,including those of the position and momentum,are studied and discussed in detail.The results provide valuable guidance for the design of STCF detector. 展开更多
关键词 BESIII experiment STCF hyperon CP violation detector resolution unbiased test
在线阅读 下载PDF
HPLC-ELSD法同时测定脑膜炎球菌多糖疫苗中蔗糖与甘露醇的含量 被引量:1
19
作者 何刚 周冬梅 +5 位作者 赖月月 黄倩 王倩 蔡思琴 王公孝 黄放 《中国药品标准》 2025年第4期424-429,共6页
目的:建立同时测定脑膜炎球菌多糖疫苗中蔗糖与甘露醇含量的高效液相色谱-蒸发光检测法(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)。方法:采用ChromCore Sugar-10Ca(300 mm×7.8 ... 目的:建立同时测定脑膜炎球菌多糖疫苗中蔗糖与甘露醇含量的高效液相色谱-蒸发光检测法(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)。方法:采用ChromCore Sugar-10Ca(300 mm×7.8 mm)分析柱和Agilent1260高效液相色谱系统,以超纯水为流动相,等度洗脱,体积流速为0.5 mL·min^(-1),进样体积为50μL,柱温为80℃,检测器为ELSD(以氮气为载气、流速3.2 L·min^(-1),漂移管温度110℃,增益值1,撞击器为“模式1”)。并对方法的适用性、专属性、重复性、中间精密度、线性和准确度等指标进行考察,用建立的方法对4批次脑膜炎球菌多糖疫苗供试品中的蔗糖及甘露醇含量进行测定。结果:所建立方法可将蔗糖与甘露醇有效分离,方法系统适用性良好;专属性验证表明空白溶液在目标峰积分范围内无干扰峰;蔗糖与甘露醇的分离度>2.0;6次重复进样蔗糖与甘露醇峰面积相对标准偏差(relative standard deviation,RSD)分别为0.44%和0.38%;中间精密度试验结果显示,高、中、低浓度蔗糖与甘露醇均RSD<2.00%;线性回归结果显示,蔗糖与甘露醇含量在12.5~150.0μg·mL^(-1)范围内时,与峰面积均存在良好的线性关系,均R^(2)>0.99;蔗糖加样回收率范围为95.74~99.33%,甘露醇加样回收率范围为94.37~98.85%,准确度良好;4批次供试品蔗糖含量均值为12313μg·mL^(-1),RSD为1.07%;甘露醇含量均值为24403μg·mL^(-1),RSD为0.48%。结论:所建立的HPLC-ELSD法可同时测定脑膜炎球菌多糖疫苗中蔗糖与甘露醇含量,经验证各项指标均符合要求,具有操作简便、准确性高、重现性好、线性范围宽及环境友好等优点,可广泛适用脑膜炎球菌多糖疫苗蔗糖与甘露醇的含量测定。 展开更多
关键词 药用辅料 蔗糖 甘露醇 高效液相色谱-蒸发光散射检测法(HPLC-elsd) 含量测定 脑膜炎球菌多糖疫苗
暂未订购
经典名方半夏泻心汤ELSD指纹图谱建立及多成分定量分析
20
作者 钱碧坤 相雨 +1 位作者 张誉晴 陈基辉 《广东化工》 2025年第11期158-161,共4页
目的:本研究旨在构建基于UPLC-ELSD联用技术的半夏泻心汤质量控制体系,实现指纹图谱构建与多指标成分同步定量分析。方法:色谱分析采用Waters ACQUITY UPLC H-Class系统耦合ELSD检测器。方法学参数设定如下:(1)分离条件:CORTECS C_(18)... 目的:本研究旨在构建基于UPLC-ELSD联用技术的半夏泻心汤质量控制体系,实现指纹图谱构建与多指标成分同步定量分析。方法:色谱分析采用Waters ACQUITY UPLC H-Class系统耦合ELSD检测器。方法学参数设定如下:(1)分离条件:CORTECS C_(18)反相柱(3.0×100 mm,2.7μm),二元梯度洗脱(流动相A:乙腈,B:超纯水),洗脱程序分五阶段(0 min~11 min维持19%A;11 min~16 min梯度升至25%A;16 min~34 min过渡至30%A;34 min~47 min提升至44%A;47 min~60 min终升至80%A);(2)运行参数:柱温30℃,流速0.4 mL·min^(-1),载气压力2.0 mL·min^(-1),检测器漂移管加热至95℃;(3)样品检测:15批次样品经处理制备后进样,采用《中药色谱指纹图谱评价系统》(2012版)进行相似度计算,并定量分析人参皂苷Re、Rg1、Rb1三类指标成分。结果:(1)特征图谱:15批次物质基准的ELSD色谱图显示18个共有特征峰,批次间相似度达0.90以上;(2)成分鉴定:通过对照品比对确认8个人参皂苷类化合物,其中人参皂苷Re、Rg1、Rb1的定量分析结果分别为0.36 mg·g^(-1)~0.70 mg·g^(-1)、0.47 mg·g^(-1)~0.90 mg·g^(-1)、0.42 mg·g^(-1)~0.81 mg·g^(-1);(3)方法验证:系统适应性、精密度及重复性试验均良好。结论:本研究开发的质量控制策略兼具指纹图谱整体性与多指标定量特异性,为半夏泻心汤物质基准的质量评价及经典名方制剂开发提供了可推广的分析方法。 展开更多
关键词 经典名方 半夏泻心汤 elsd 指纹图谱 相似度评价
在线阅读 下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部