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基于OPGD和GTWR耦合模型分析NDVI时空演变——以粤港澳大湾区为例
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作者 汤雄 李恒凯 张霆 《节水灌溉》 北大核心 2026年第1期97-105,共9页
归一化植被指数(NDVI)是植被覆盖动态的表征指数之一,可以有效反映区域水土保持状况、生态系统健康和农业生产力,对协调生态保护与区域高质量发展至关重要。由于现有研究集中于单一驱动因素,不足以阐释NDVI时空演变中多要素协同驱动的... 归一化植被指数(NDVI)是植被覆盖动态的表征指数之一,可以有效反映区域水土保持状况、生态系统健康和农业生产力,对协调生态保护与区域高质量发展至关重要。由于现有研究集中于单一驱动因素,不足以阐释NDVI时空演变中多要素协同驱动的复杂非线性过程,因此该研究综合运用变异系数法、最优参数地理探测器(OPGD)和时空地理加权回归(GTWR)等方法,量化分析2000-2020年粤港澳大湾区NDVI的时空演变特征及其驱动机制。结果表明:基于变异系数法,粤港澳大湾区NDVI在时间上呈波动上升趋势,植被状况总体上有显著改善;在空间分布上呈现出显著的空间异质性。基于OPGD的单因子探测识别出人口密度与夜间灯光亮度值为影响NDVI空间分异的主要驱动因子;交互因子探测进一步表明,人口密度、夜间灯光亮度值与温度等关键因子间的交互作用显著增强了对NDVI变化的解释力。GTWR模型有效揭示了各驱动因子对NDVI影响的时空非平稳性特征,各因子回归系数在空间上存在显著差异。研究为高强度人类活动区域的生态系统水土监测与调控机制设计提供了理论依据,有效支撑粤港澳大湾区水土保持政策的规划与管理。 展开更多
关键词 NDVI 最优参数地理探测器 GTWR 冷热点分析
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天山北坡耕地利用转型时空演变及其驱动因素研究——基于OPGD模型
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作者 马雪梦 阿依吐尔逊·沙木西 赵俊 《资源开发与市场》 2026年第1期54-64,共11页
为优化耕地利用格局,摸清耕地利用的发展态势,为天山北坡地区耕地保护提供依据。从空间与功能形态转型两个视角,运用熵值法、空间自相关与OPGD模型等方法,借助ArcGIS空间表达技术,揭示耕地利用转型的时空演变格局及其驱动因素。结果表明... 为优化耕地利用格局,摸清耕地利用的发展态势,为天山北坡地区耕地保护提供依据。从空间与功能形态转型两个视角,运用熵值法、空间自相关与OPGD模型等方法,借助ArcGIS空间表达技术,揭示耕地利用转型的时空演变格局及其驱动因素。结果表明:①从时空变化看,2000—2020年空间形态转型水平逐渐提高、功能形态转型呈平缓波动趋势,高值区由中部核心城市逐步向边缘城市扩散,综合转型指数呈上涨趋势,耕地利用转型格局形成以中部盆地平原、西部伊犁河谷平原为主的高值区与东部山地低值区并存的空间格局;②从空间集聚特征看,空间形态转型呈先减少后增加最后减少的趋势,于2010年达到峰值,聚集区域由双核逐步向多核发展,功能形态转型呈先升高后再降低最后趋于平缓的态势,聚集区总体位于中部地区;③从驱动因素看,城镇化率、地均固定资产投资与人口密度对耕地利用转型的影响更为突出。综上,天山北坡耕地利用转型结果正逐步向好,但仍存在区域间差异,应利用人口与社会经济因素对转型的驱动作用,促进耕地资源的可持续利用,实现耕地利用优化转型。 展开更多
关键词 耕地利用格局 空间形态 功能形态 驱动因素 opgd模型 天山北坡
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Response estimation and evaluation of direct-conversion dual-layer perovskite X-ray detectors:a numerical study with a cascaded signal model
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作者 Han Cui Yu-Hang Tan +7 位作者 Xin Zhang Hao-Di Wu Ting Su Jiong-Tao Zhu Hai-Rong Zheng Dong Liang Xiang-Ming Sun Yong-Shuai Ge 《Nuclear Science and Techniques》 2026年第2期156-168,共13页
This study aims to investigate the responses of a perovskite-based direct-conversion dual-layer flat-panel detector(DL-FPD)numerically.To this end,the X-ray sensitivity,spatial resolution quantified by the modulation ... This study aims to investigate the responses of a perovskite-based direct-conversion dual-layer flat-panel detector(DL-FPD)numerically.To this end,the X-ray sensitivity,spatial resolution quantified by the modulation transfer function(MTF),and detective quantum efficiency(DQE)of the DL-FPD are evaluated numerically using a linear cascade model.In addition,both the single-crystal(SC)and polycrystalline(PC)structures of MAPbI_(3)are investigated,along with various other key parameters such as the material thickness,electric field strength,X-ray beam spectrum,and electronic readout noise.The results demonstrate that SC perovskite consistently exhibits better performance than PC perovskite owing to fewer material defects.Increasing the layer thickness may decrease the MTF,but can also enhance the sensitivity and DQE.Moreover,appropriately increasing the external electric field within the material can improve the sensitivity,MTF,and DQE.Finally,reducing the electronic readout noise can significantly enhance the DQE for low-dose imaging.This study demonstrates the potential of high-quality dual-energy X-ray imaging using direct-conversion perovskite DL-FPDs. 展开更多
关键词 X-ray imaging Dual-layer flat-panel detector Perovskite X-ray detector
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Prototype of front-end electronics based on FPGA-ADC for TOF PET detector applications
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作者 Song-Qing Liu Bo Wang +2 位作者 Wei-Wei Xu Xin-Sheng Wang Kun Hu 《Nuclear Science and Techniques》 2026年第2期134-142,共9页
Traditional digitizers for signal readout of PET detectors are based on commercial analog-to-digital converters(ADC).However,the cost and power consumption of an entire electronic readout system based on digitizers fo... Traditional digitizers for signal readout of PET detectors are based on commercial analog-to-digital converters(ADC).However,the cost and power consumption of an entire electronic readout system based on digitizers for a PET scanner are high.To address this problem,a soft-core ADC based on a field-programmable gate array(FPGA)was proposed.An FPGA-based ADC(FPGA-ADC)combines low loss and high performance.To achieve good performance,the FPGA-ADC requires three calibrations:time-to-digital converter(TDC)length calibration,TDC alignment calibration,and TDC-to-ADC calibration.A prototype front-end electronics based on FPGA-ADC was built to evaluate the performance of time-of-flight positron emission tomography(TOF PET)detectors.Each PET detector consists of a LYSO crystal single-ended coupled to a silicon photomultiplier(SiPM).The experimental results show that the full-width at half-maximum(FWHM)energy resolution for 511 keV gamma photons after saturation correction of the SiPM was 12.3%.The FWHM coincidence timing resolution(CTR)of the TOF PET detector with the readout of the front-end electronic prototype is 385.2 ps.FPGA-ADCbased front-end electronics are very promising for multichannel,low-cost,highly integrated,and power-efficient readout electronic systems for radiation detector applications. 展开更多
关键词 Front-end electronics Analog-to-digital converter Radiation detector PET FPGA
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Unity-based virtual reality for detector and event visualization in JUNO experiment
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作者 Kai-Xuan Huang Tian-Zi Song +4 位作者 Yu-Ning Su Cheng-Xin Wu Xue-Sen Wang Yu-Mei Zhang Zheng-Yun You 《Nuclear Science and Techniques》 2026年第4期127-138,共12页
Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effect... Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effects and flexible extensibility for visualization in HEP experiments.In this study,we present a VR-based method for detector and event displays in the Jiangmen Underground Neutrino Observatory(JUNO)experiment.This method shares the same detector geometry descriptions and event data model as those in the offline software and provides the necessary data conversion interfaces.The VR methodology facilitates an immersive exploration of the virtual environment in JUNO,enabling users to investigate the detector geometry,visualize event data,and tune the detector simulation and event reconstruction algorithms.Additionally,this approach supports applications in data monitoring,physics data analysis,and public outreach initiatives. 展开更多
关键词 Virtual reality Event display UNITY detector geometry JUNO
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Preparation and measurement of an^(37)Ar source for liquid xenon detector calibration
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作者 Xu‑Nan Guo Chang Cai +8 位作者 Fei Gao Yang Lei Kai‑Hang Li Chun‑Lei Su Ze‑Peng Wu Xiang Xiao Ling‑Feng Xie Yi‑Fei Zhao Xiao‑Peng Zhou 《Nuclear Science and Techniques》 2026年第1期202-211,共10页
We present the preparation and measurement of the radioactive isotope^(37)Ar,which was produced using thermal neutrons from a reactor,as a calibration source for liquid xenon time projection chambers.^(37)Ar is a low-... We present the preparation and measurement of the radioactive isotope^(37)Ar,which was produced using thermal neutrons from a reactor,as a calibration source for liquid xenon time projection chambers.^(37)Ar is a low-energy calibration source with a half-life of 35.01 days,making it suitable for calibration in the low-energy region of liquid xenon dark-matter experiments.Radioactive isotope^(37)Ar was produced by irradiating ^(36)Ar with thermal neutrons.It was subsequently measured in a gaseous xenon time projection chamber(GXe TPC)to validate its radioactivity.Our results demonstrate that^(37)Ar is an effective and viable calibration source that offers precise calibration capabilities in the low-energy domain of xenon-based detectors. 展开更多
关键词 ^(37)Ar Gaseous xenon detector LOW-ENERGY Calibration source
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Superconducting nanowire single photon detector with efficiency over 90% at 2μm wavelength
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作者 Zhen Wan Jia Huang +6 位作者 Guangzhao Xu Yu Ding Xiaoyu Liu Yiming Pan Hongxin Xu Hao Li Lixing You 《Chinese Physics B》 2026年第1期306-312,共7页
We here report a high system detection efficiency(SDE)superconducting single-photon detector(SSPD)at 2μm wavelength.The device integrates a SiO_(2)/Ta_(2)O_(5)distributed Bragg reflector(DBR)and a sandwich-structured... We here report a high system detection efficiency(SDE)superconducting single-photon detector(SSPD)at 2μm wavelength.The device integrates a SiO_(2)/Ta_(2)O_(5)distributed Bragg reflector(DBR)and a sandwich-structured double-layer NbN nanowire to enhance the optical absorption efficiency.A cold development technique is implemented to optimize the superconducting nanowires with sub-40-nm linewidths,thus enhancing the intrinsic detection efficiency(IDE).The fabricated SSPD shows an SDE exceeding 90% at 2μm wavelength.Moreover,the detector allows an operational working temperature of 2.2 K provided by a compact GM cryo-cooler.This detector delivers excellent performance at the 2μm wavelength,and its optimized structural design implies promising potential for extending detection toward longer infrared bands.It thus holds value for advancing high-sensitivity quantum technologies,mid-infrared optical communications,and dark matter detection research. 展开更多
关键词 superconducting single-photon detectors(SSPDs) system detection efficiency near infrared
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How Does Urban Public Transit Accessibility Affect Housing Prices?A Comprehensive Analysis with Geographical Detector Combined and Geographically Weighted Regression
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作者 TANG Jingjing HAN Huiran +3 位作者 YANG Chengfeng XU Lingyi GENG Hui LI Lei 《Chinese Geographical Science》 2026年第1期127-143,共17页
The accessibility of urban public transit directly influences residents’quality of life,travel behavior,and social equity.Its correlation with housing prices has garnered significant attention across disciplines such... The accessibility of urban public transit directly influences residents’quality of life,travel behavior,and social equity.Its correlation with housing prices has garnered significant attention across disciplines such as geography,economics,and urban planning.Although much existing research focuses on the impact of individual transportation facilities on housing prices,there is a notable gap in comprehensive analyses that assess the influence of overall urban transit accessibility on housing market dynamics.This study selected the main urban area of Hefei,China,as a case to investigate the spatial distribution of housing prices and evaluate public transit accessibility in 2022.Employing techniques such as the optimized parameter geographical detector and local spatial regression models,the study aimed to elucidate the effects and underlying mechanisms of urban transit accessibility on housing prices.The findings revealed that:1)housing prices in Hefei exhibited a clustered spatial pattern,with high prices concentrated in the city center and lower prices in peripheral areas,forming three distinct high-price hotspots with a‘belt-like’distribution;2)public transit accessibility showed a‘coreperiphery’structure,with accessibility declining in a‘circumferential’pattern around the city center.Based on the‘housing price-accessibility’dimension,four categories were identified:high price-high accessibility(37.25%),high price-low accessibility(19.07%),low price-high accessibility(21.95%),and low price-low accessibility(21.73%);3)the impact of transit accessibility on housing prices was spatially heterogeneous,with bus travel showing the strongest explanatory power(0.692),followed by automobile,subway,and bicycle travel.The interaction of these transportation modes generated a synergistic effect on housing price differentiation,with most influencing factors contributing more than 25%.These findings offer valuable insights for optimizing the spatial distribution of public transit infrastructure and improving both urban housing quality and residents’living standards. 展开更多
关键词 public transit accessibility housing prices geographically weighted regression geographical detector Hefei City China
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基于OPGD的嘉陵江流域潜在蒸散量时空演变及其驱动机制
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作者 祁欢欢 罗君 +1 位作者 刘城 戴前坤 《绿色科技》 2026年第1期220-224,共5页
为揭示2001-2022年嘉陵江流域潜在蒸散量的时空演变规律及主控因子,本研究以该流域逐月潜在蒸散量数据为基础,结合数字高程模型、人口密度、气温、降水等8个自然与人文因子,采用最优参数地理探测器量化各因子对潜在蒸散量的解释力及交... 为揭示2001-2022年嘉陵江流域潜在蒸散量的时空演变规律及主控因子,本研究以该流域逐月潜在蒸散量数据为基础,结合数字高程模型、人口密度、气温、降水等8个自然与人文因子,采用最优参数地理探测器量化各因子对潜在蒸散量的解释力及交互作用。结果表明:时间上,该流域年均潜在蒸散量呈不显著缓慢下降趋势,多年均值为825.227 mm;空间上,潜在蒸散量呈南部偏高、西部偏低、北部西低东高的格局,最高值集中于绵阳市西北部,最低值分布于西部高海拔区,22年空间格局稳定性强;驱动因子中,气温与数字高程模型(Digital Elevation Model,DEM)为潜在蒸散量空间分异的主导因子,所有因子组合均表现为“双因子增强”效应,其中气温与DEM交互解释力最高。 展开更多
关键词 嘉陵江流域 潜在蒸散量 最优参数地理探测器 驱动因子
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Lattice Anchoring Stabilizesα-FAPbI_(3) Perovskite for High-Performance X-Ray Detectors
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作者 Yu-Hua Huang Su-Yan Zou +5 位作者 Cong-Yi Sheng Yu-Chuang Fang Xu-Dong Wang Wei Wei Wen-Guang Li Dai-Bin Kuang 《Nano-Micro Letters》 2026年第1期337-354,共18页
Formamidinium lead iodide(FAPbI_(3))perovskite exhibits an impressive X-ray absorption coefficient and a large carrier mobility-lifetime product(μτ),making it as a highly promising candidate for X-ray detection appl... Formamidinium lead iodide(FAPbI_(3))perovskite exhibits an impressive X-ray absorption coefficient and a large carrier mobility-lifetime product(μτ),making it as a highly promising candidate for X-ray detection application.However,the presence of larger FA^(+)cation induces to an expansion of the Pb-I octahedral framework,which unfortunately affects both the stability and charge carrier mobility of the corresponding devices.To address this challenge,we develop a novel low-dimensional(HtrzT)PbI_(3) perovskite featuring a conjugated organic cation(1H-1,2,4-Triazole-3-thiol,HtrzT^(+))which matches well with theα-FAPbI_(3) lattices in two-dimensional plane.Benefiting from the matched lattice between(HtrzT)PbI_(3) andα-FAPbI_(3),the anchored lattice enhances the Pb-I bond strength and effectively mitigates the inherent tensile strain of theα-FAPbI_(3) crystal lattice.The X-ray detector based on(HtrzT)PbI_(3)(1.0)/FAPbI_(3) device achieves a remarkable sensitivity up to 1.83×10^(5)μC Gy_(air)^(−1) cm^(−2),along with a low detection limit of 27.6 nGy_(air) s^(−1),attributed to the release of residual stress,and the enhancement in carrier mobility-lifetime product.Furthermore,the detector exhibits outstanding stability under X-ray irradiation with tolerating doses equivalent to nearly 1.17×10^(6) chest imaging doses. 展开更多
关键词 α-FAPbI_(3)perovskite Conjugated organic cation Lattice anchoring Phase stability X-ray detectors
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Ultralow dark current soft X-ray detectors based on lead-free double perovskite Cs_(2)AgBiBr_(6)
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作者 Long Cheng Lijuan Huang +15 位作者 Mulin Sun Ying Meng Yuan Li Tianyu Liu Pengju Tan Mingzhu Hu Huaqing Yang Xiaolan Ma Shunjie Yu Xiaohu Hou Yong Guan Junfa Zhu Xiaosong Liu Yu Li Shibing Long Qin Hu 《Journal of Semiconductors》 2026年第1期109-116,共8页
Soft X-ray detectors play a vital role in materials science,high-energy physics and medical imaging.Cs_(2)AgBiBr_(6),a lead-free double perovskite,has gained attention for its excellent optoelectronic properties,stabi... Soft X-ray detectors play a vital role in materials science,high-energy physics and medical imaging.Cs_(2)AgBiBr_(6),a lead-free double perovskite,has gained attention for its excellent optoelectronic properties,stability,and nontoxicity.However,its fast crystallization and requirement for high-temperature annealing(>250℃)often lead to inferior film quality,limiting its application in flexible devices.This study introduces an alloying strategy that significantly improves the quality of Cs_(2)AgBiBr_(6)thin films annealed at a reduced temperature of 150℃.Devices based on the alloyed thin films exhibit an ultra-low dark current of 0.32 nA·cm^(-2)and a quantum efficiency of 725%.Furthermore,the first successful integration of Cs_(2)AgBiBr_(6)with a thinfilm transistor backplane demonstrates its superior imaging performance,indicating that Cs_(2)AgBiBr_(6)is a promising material for next-generation soft X-ray sensors. 展开更多
关键词 soft X-ray detector lead-free double perovskite Cs_(2)AgBiBr_(6) ALLOYING IMAGING
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VMFD:Virtual Meetings Fatigue Detector Using Eye Polygon Area and Dlib Shape Indicator
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作者 Hafsa Sidaq Lei Wang +3 位作者 Sghaier Guizani Hussain Haider Ateeq Ur Rehman Habib Hamam 《Computers, Materials & Continua》 2026年第3期672-688,共17页
Numerous sectors,such as education,the IT sector,and corporate organizations,transitioned to virtual meetings after the COVID-19 crisis.Organizations now seek to assess participants’fatigue levels in online meetings ... Numerous sectors,such as education,the IT sector,and corporate organizations,transitioned to virtual meetings after the COVID-19 crisis.Organizations now seek to assess participants’fatigue levels in online meetings to remain competitive.Instructors cannot effectively monitor every individual in a virtual environment,which raises significant concerns about participant fatigue.Our proposed system monitors fatigue,identifying attentive and drowsy individuals throughout the online session.We leverage Dlib’s pre-trained facial landmark detector and focus on the eye landmarks only,offering a more detailed analysis for predicting eye opening and closing of the eyes,rather than focusing on the entire face.We introduce an Eye Polygon Area(EPA)formula,which computes eye activity from Dlib eye landmarks by measuring the polygonal area of the eye opening.Unlike the Eye Aspect Ratio(EAR),which relies on a single distance ratio,EPA adapts to different eye shapes(round,narrow,or wide),providing a more reliable measure for fatigue detection.The VMFD system issues a warning if a participant remains in a fatigued condition for 36 consecutive frames.The proposed technology is tested under multiple scenarios,including low-to high-lighting conditions(50-1400 lux)and both with and without glasses.This study builds an OpenCV application in Python,evaluated using the iBUG 300-W dataset,achieving 97.5%accuracy in detecting active participants.We compare VMFD with conventional methods relying on the EAR and show that the EPA technique performs significantly better. 展开更多
关键词 Fatigue detector human-computer interaction(HCI) eye blink active and inactive behavior online meeting automatic fatigue alert
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基于OPGD模型的昆明市植被NPP时空演变及驱动机制 被引量:6
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作者 张仲芝 赵俊三 +3 位作者 陈国平 王琳 林伊琳 张丹丹 《水土保持学报》 北大核心 2025年第2期298-308,共11页
[目的]厘清植被净初级生产力(NPP)演变规律及驱动因素对测算植被的固碳能力和支撑碳交易等具有重要的意义。[方法]基于MODIS-NPP数据,运用Theil-Sen Median趋势法和Mann-Kendall检验法分析2001—2020年昆明市植被NPP的时空动态特征,并... [目的]厘清植被净初级生产力(NPP)演变规律及驱动因素对测算植被的固碳能力和支撑碳交易等具有重要的意义。[方法]基于MODIS-NPP数据,运用Theil-Sen Median趋势法和Mann-Kendall检验法分析2001—2020年昆明市植被NPP的时空动态特征,并采用参数最优地理检测器(OPGD)模型揭示气候、地形和人为因素对植被NPP的影响机制。[结果] 1)昆明市植被NPP在空间上呈“西高-东低”的分布格局,并以7.08 g/(m^(2)·a)(以C计)的速度显著增加。2)植被景观破碎化程度加剧,在空间上破碎度与植被NPP错位分布,其对植被NPP时空分布有显著负向作用。3)植被NPP变化是气候、人为活动和地形因素共同作用的结果。其中,核归一化植被指数(kNDVI)、地表温度、植被景观破碎度解释力强于气温、降水和坡度等因子,因子间交互作用能更好地解释植被NPP的时空变化。[结论]昆明市2001—2020年植被NPP总体呈增加态势,但主城区少部分地区形势不容乐观,未来应综合统筹,科学推进生态保护与社会经济的协调发展。 展开更多
关键词 植被净初级生产力 参数最优地理探测器模型(opgd) 双变量空间自相关 核归一化植被指数(kNDVI) 昆明市
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基于kNDVI和OPGD的贵阳市近33 a植被变化及影响因素分析 被引量:1
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作者 赵祖伦 蒋啸 +4 位作者 苏印 尹林江 罗婷 赵卫权 罗军华 《环境科学》 北大核心 2025年第9期5839-5849,共11页
植被指数是监测陆地生态系统变化的重要指标,揭示植被变化的时空特征和潜在影响因子对于促进区域生态环境保护和管理具有重要意义.基于1990~2023年8期Landsat遥感影像,利用遥感云计算平台(GEE)计算贵阳市核归一化植被指数(kNDVI),并采用... 植被指数是监测陆地生态系统变化的重要指标,揭示植被变化的时空特征和潜在影响因子对于促进区域生态环境保护和管理具有重要意义.基于1990~2023年8期Landsat遥感影像,利用遥感云计算平台(GEE)计算贵阳市核归一化植被指数(kNDVI),并采用Theil-Sen+Mann-Kendall趋势分析法研究植被kNDVI变化趋势及显著性水平,然后结合Hurst指数评估植被kNDVI的未来趋势和持续性特征,最后基于最优参数地理探测器(OPGD)分析植被kNDVI的空间分异驱动机制.结果表明:①1990~2023年贵阳市植被kNDVI整体呈现4个阶段变化的波动上升趋势,空间上呈现北高南低的分布格局,存在明显的空间分异性;②过去33 a贵阳市74.62%的区域植被覆盖呈现改善特征,25.14%的区域呈现退化特征;③Hurst指数均值为0.6102,表现为弱持续性特征,贵阳市植被kNDVI具有持续改善的未来变化趋势;④土地利用因子(0.2312)对于植被kNDVI空间分异的解释力最强,因子交互作用均表现为非线性增强与双因子增强,土地利用与其它因子协同作用更有利于解释植被kNDVI空间分异. 展开更多
关键词 核归一化植被指数(kNDVI) 非线性趋势 HURST指数 最优参数地理探测器(opgd) 贵阳市
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RAA-DETECTOR布鲁氏菌核酸快速检测方法的建立及应用 被引量:1
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作者 张雲龙 王靖雷 +8 位作者 朱亚杰 张明洁 康澳 周翔 魏凯 曹洪防 李强 王勇 苏峰 《畜牧兽医学报》 北大核心 2025年第10期5115-5124,共10页
本研究旨在建立不依赖于专业设备的布鲁氏菌病高效快速检测体系,实现临床样本40 min内快速检测。首先比对不同布鲁氏菌的基因组,找到布鲁氏菌的核心共有序列,以布鲁氏菌S2菌株基因SEQ NO.6(CN 105018489A)为模板构建阳性质粒,用于后续试... 本研究旨在建立不依赖于专业设备的布鲁氏菌病高效快速检测体系,实现临床样本40 min内快速检测。首先比对不同布鲁氏菌的基因组,找到布鲁氏菌的核心共有序列,以布鲁氏菌S2菌株基因SEQ NO.6(CN 105018489A)为模板构建阳性质粒,用于后续试验;通过原核表达的方法分别表达CRISPR/AsCas12a和LwaCas13a蛋白;通过重组酶介导的等温核酸扩增技术(RAA)、体外表达技术结合AsCas12a/LwaCas13a的DETECTOR系统,优化高效检测体系、通过无限稀释法等进行检测。结果发现,设计的RAA引物能够有效扩增样本中的DNA片段,DETECTOR得到最佳反应的AsCas12a浓度为200 nmol·L^(-1),gRNA为200 nmol·L^(-1);Cas13a浓度为300 nmol·L^(-1),crRNA浓度为400 nmol·L^(-1);RAA-AsCas12a和RAA-LwaCas13a的检测体系的最佳反应时间为35 min,侧平流式纸条的最佳检测时间为20 min,细菌最低检测浓度为10 Copies·μL^(-1),且具有较好的检测特异性,临床样本的检测试验表明两种方法均有较好的重复性和检测精度,但LwaCas13a的精测精度更高,但二者无显著差异。该研究建立了2种RAA-CRISPR/Cas布鲁氏菌核酸快速检测体系,在临床上可获得较好的应用,RAA-LwaCas13a的检测方法精度更高。 展开更多
关键词 布鲁氏菌 RAA detector系统 SHERLOCK系统 AsCas12a LwaLwaCas13a
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CMOS direct conversion X-ray detector coupled with fluorinated liquid 被引量:1
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作者 Shi-Hua Liu Chao-Song Gao +5 位作者 Xin Zhang Xiang-Ming Sun Meng Wu Zhi-Hui Han Tong Wan Yong-Shuai Ge 《Nuclear Science and Techniques》 2025年第1期59-68,共10页
X-ray detectors show potential applications in medical imaging,materials science,and nuclear energy.To achieve high detection efficiency and spatial resolution,many conventional semiconductor materials,such as amorpho... X-ray detectors show potential applications in medical imaging,materials science,and nuclear energy.To achieve high detection efficiency and spatial resolution,many conventional semiconductor materials,such as amorphous selenium,cadmium telluride zinc,and perovskites,have been utilized in direct conversion X-ray detectors.However,these semiconductor materials are susceptible to temperature-induced performance degradation,crystallization,delamination,uneven lattice growth,radiation damage,and high dark current.This study explores a new approach by coupling an FC40 electronic fluorinated liquid with a specialized high-resolution and high-readout-speed complementary metal-oxide-semiconductor(CMOS)pixel array,specifically the Topmetal II−chip,to fabricate a direct conversion X-ray detector.The fluorinated liquid FC40(molecular formula:C_(21)F_(48)N_(2))is an electronic medium that is minimally affected by temperature and displays no issues with uniform conductivity.It exhibits a low dark current and minimal radiation damage and enables customizable thickness in X-ray absorption.This addresses the limitations inherent in conventional semiconductor-based detectors.In this study,simple X-ray detector imaging tests were conducted,demonstrating the excellent coupling capability between FC40 electronic fluorinated liquid and CMOS chips by the X-ray detector.A spatial resolution of 4.0 lp/mm was measured using a striped line par card,and a relatively clear image of a cockroach was displayed in the digital radiography imaging results.Preliminary test results indicated the feasibility of fabricating an X-ray detector by combining FC40 electronic fluorinated liquid and CMOS chips.Owing to the absence of issues related to chip-material coupling,a high spatial resolution could be achieved by reducing the chip pixel size.This method presents a new avenue for studies on novel liquid-based direct conversion X-ray detectors. 展开更多
关键词 FC40 electronic fluorinated liquid CMOS pixel chip X-ray detector Spatial resolution
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Optimizing neon-based gas mixtures for two-stage amplification fast-timing Micromegas detectors
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作者 Yue Meng Xu Wang +3 位作者 Jianbei Liu Ming Shao Zhiyong Zhang Yi Zhou 《中国科学技术大学学报》 北大核心 2025年第4期59-66,58,I0002,共10页
The PICOSEC Micromegas(MM)is a precise timing gaseous detector based on a Cherenkov radiator coupled with a semi-transparent photocathode and an MM amplifying structure.It features a two-stage amplification process th... The PICOSEC Micromegas(MM)is a precise timing gaseous detector based on a Cherenkov radiator coupled with a semi-transparent photocathode and an MM amplifying structure.It features a two-stage amplification process that leads to a significant deterioration of non-uniformity when scaling up to larger areas.Since the performance of gaseous detectors is highly dependent on the choice of working gas,optimizing the gas mixture offers a promising solution to improve the uniformity performance.This paper addresses these challenges through a combined approach of simulation based on Garfield++and experimental studies.The simulation investigates the properties of different mixing fractions of gas mixtures and their impact on detector performance,including gain uniformity and time resolution.To verify the simulation results,experimental tests were conducted using a multi-channel PICOSEC MM prototype with different gas mixtures.The experimental results are consistent with the findings of the simulation,indicating that a higher concentration of neon significantly improves the detector’s gain uniformity.Furthermore,the influence of gas mixtures on time resolution was explored as a critical performance indicator.The study presented in this paper offers valuable insights for improving uniformity in large-area PICOSEC MM detectors and optimizing overall performance. 展开更多
关键词 gaseous detectors MICROMEGAS timing detectors fast-timing Micromegas Monte Carlo simulation gain uniformity
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A prototype of 2μm balanced detector for space-borne Coherent Doppler Wind Lidar
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作者 CHEN Bao-Lin JIN Ge +2 位作者 WANG Chong CHEN Lian 《红外与毫米波学报》 北大核心 2025年第6期1021-1029,共9页
Despite rapid advancements in lidar technology,extremely long-range observation remains a signifi⁃cant challenge.Recently,2μm lasers have demonstrated a potential to be applied in CDWL(Coherent Doppler Wind Lidar)sys... Despite rapid advancements in lidar technology,extremely long-range observation remains a signifi⁃cant challenge.Recently,2μm lasers have demonstrated a potential to be applied in CDWL(Coherent Doppler Wind Lidar)system,for its high atmospheric penetration capability through the atmosphere and high potential la⁃ser power.In this study,we present a 2μm balanced detector that consists of a pair of commercial positive-intrin⁃sic-negative(PIN)diodes with a low-noise transimpedance circuit.To meet the high bandwidth requirements,the highspeed transimpedance circuit and bias voltage tuning method were utilized to overcome the large capaci⁃tance of PIN diodes.The circuit transfer function,stability analysis and noise calculation have been studied.The detector was co-packaged with a data acquisition module for convenient data transmission and bias voltage con⁃trol.The characteristics of the detector,including bandwidth,noise and bias voltage influence,are evaluated in laboratory.Results show that the RMS value of the balanced detector background noise is 539μV and the band⁃widths of the two diodes are 110.8 MHz and 110.3 MHz,respectively.The evaluation results show that the bal⁃anced detector meets the wind measurement requirements and allows for a 1.45×increase in bandwidth through bi⁃as voltage tuning.Our work offers insights into lidar detector design and bandwidth enhancement,providing a valuable reference for researchers and professionals in the field.More importantly,it lays a critical foundation for fu⁃ture ultra-long-range and space-borne 2μm coherent wind lidar systems by addressing key device-level challenges. 展开更多
关键词 balanced detector transimpedance circuit CDWL laser detection
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Study of bonding layer for integrated structure of space gravitational wave detector telescope
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作者 ZHAO Hong-chao LIU Chang +2 位作者 ZHOU Wen-ke ZHU Han-bin CHEN Wen-duo 《中国光学(中英文)》 北大核心 2025年第3期715-724,共10页
To detect space gravitational waves in the extremely low-frequency band,the telescope and optic-al platform require high stability and reliability.However,the cantilevered design presents challenges,espe-cially in the... To detect space gravitational waves in the extremely low-frequency band,the telescope and optic-al platform require high stability and reliability.However,the cantilevered design presents challenges,espe-cially in the glass-metal hetero-bonding process.This study focuses on the analysis and experimental re-search of the bonding layer in the integrated structure.By optimizing the structural configuration and select-ing suitable bonding processes,the reliability of the telescope system is enhanced.The research indicates that using J-133 adhesive achieves the best performance,with a bonding layer thickness of 0.30 mm and a metal substrate surface roughness of Ra 0.8.These findings significantly enhance the reliability of the optical sys-tem while minimizing potential risks. 展开更多
关键词 space gravitational-wave detector integrated structure glass-metal hetero-bonding
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Unbiased test of the hyperon CP violation in terms of detector resolution at STCF
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作者 Ming Xu Liang Liu +2 位作者 Yupeng Pei Xiaorong Zhou Yingchun Zhu 《中国科学技术大学学报》 北大核心 2025年第4期41-47,40,I0002,共9页
The study of the charge conjugation and parity(CP)violation of hyperon is the precision frontier for probing possible new CP violation sources beyond the standard model(SM).With the large number of quantum entangled h... The study of the charge conjugation and parity(CP)violation of hyperon is the precision frontier for probing possible new CP violation sources beyond the standard model(SM).With the large number of quantum entangled hyperonantihyperon pairs to be produced at Super Tau-Charm Facility(STCF),the CP asymmetry of hyperon is expected to be tested with a statistical sensitivity of 10^(−4) or even better.To cope with the statistical precision,the systematic effects from various aspects are critical and need to be studied in detail.In this paper,the sensitivity effects on the CP violation parameters associated with the detector resolution,including those of the position and momentum,are studied and discussed in detail.The results provide valuable guidance for the design of STCF detector. 展开更多
关键词 BESIII experiment STCF hyperon CP violation detector resolution unbiased test
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