Tisplatin is one of the valuable icancer agents against several types of neoplasm. However, nephrotoxicity is the major adverse effect representing in cisplatin therapy. In this study, the animal tests detecting prote...Tisplatin is one of the valuable icancer agents against several types of neoplasm. However, nephrotoxicity is the major adverse effect representing in cisplatin therapy. In this study, the animal tests detecting protective effects of a natural compound, Decursin, on cisplatin-induced nephrotoxicity were examined by using in vivo model. Pretreatment Decursin 10, 20 and 40 mg · kg^-1 at 48, 24 and 6 h, and administration of a single dose of Cisplatin 5.2 mg · kg^-1. Nephrotoxicity was evaluated by serum BUN and creatinine examination. There was significant difference in body weights, serum BUN and creatinine levels of the normal group. Based on the new understanding of the protective mechanisms of cisplatin-induced nephrotocivity, new strategies can be developed to prevent renal injury or to enhance recovery after cisplatin treatment.展开更多
The subacute intraperitoneal toxicity of decursin was investigated in Spargue-Dawly（SD） rats. The rats were treated with decursin at a dose of 125 and 250 mg·kg-1·day-1for 4 weeks. All animals were observe...The subacute intraperitoneal toxicity of decursin was investigated in Spargue-Dawly（SD） rats. The rats were treated with decursin at a dose of 125 and 250 mg·kg-1·day-1for 4 weeks. All animals were observed daily for clinical signs. Their body weights were recorded weekly, no mortality was observed in two doses of 125 and 250 mg·kg-1of decursin. There were no significant differences in the clinical observations, body weight, food and water consumption, hematology, blood chemistry, gross pathological examination or organ weight between control and treated animals of both sexes. In conclusion, no evidence of a subacute toxic potential was observed in this study and no indication for adverse effects was noted at a dose level of 250 mg·kg-1·day-1.展开更多
目的:探讨紫花前胡素在动脉粥样硬化疾病中的抗炎作用及其安全性,并验证其对巨噬细胞炎症反应的调节作用。方法:构建高脂饮食喂养的载脂蛋白E基因敲除(apolipoprotein E deficient, ApoE-/-)雄性小鼠动脉粥样硬化模型,通过氧化型低密度...目的:探讨紫花前胡素在动脉粥样硬化疾病中的抗炎作用及其安全性,并验证其对巨噬细胞炎症反应的调节作用。方法:构建高脂饮食喂养的载脂蛋白E基因敲除(apolipoprotein E deficient, ApoE-/-)雄性小鼠动脉粥样硬化模型,通过氧化型低密度脂蛋白(oxidized low-density lipoprotein, oxLDL)刺激RAW 264.7巨噬细胞以诱导泡沫细胞形成。采用油红O(oil red O, ORO)染色评估主动脉斑块面积减少比例。通过蛋白质印迹检测核因子κB(nuclear factorκB, NF-κB)的磷酸化水平,采用炎症因子阵列分析血清及细胞上清中炎症因子水平,并通过苏木精-伊红(hematoxylin-eosin, HE)染色评估紫花前胡素的全身毒性。结果:紫花前胡素显著减少主动脉斑块面积,在oxLDL刺激的巨噬细胞中,其可抑制NF-κB的过度活化,下调促炎因子[如白介素(interleukin, IL)-2和干扰素(interferon, IFN)-γ]的表达,同时上调抗炎因子(如IL-4和IL-10)的水平。此外,在ApoE-/-小鼠模型中,紫花前胡素同样显著降低了血清中促炎因子水平,提高抗炎因子水平。HE染色结果显示,紫花前胡素未对主要器官造成明显的病理损伤。结论:紫花前胡素通过调控巨噬细胞炎症反应发挥显著的抗炎作用,并减轻动脉粥样硬化病变,安全性较高,有望成为治疗动脉粥样硬化的潜在药物。展开更多
目的探讨紫花前胡素通过Nox1/Akt信号通路抑制口腔鳞癌细胞增殖及侵袭的作用机制。方法SCC-25组(A组)及紫花前胡素低、中、高剂量组(B1组、B2组、B3组)均取10 mL SCC-25细胞(密度为5×106/mL),置有10%胎牛血清的DMEM培养基中,分别...目的探讨紫花前胡素通过Nox1/Akt信号通路抑制口腔鳞癌细胞增殖及侵袭的作用机制。方法SCC-25组(A组)及紫花前胡素低、中、高剂量组(B1组、B2组、B3组)均取10 mL SCC-25细胞(密度为5×106/mL),置有10%胎牛血清的DMEM培养基中,分别加入紫花前胡素0,20.0,40.0,80.0μg/mL,置CO2培养箱(37℃、5%CO2、2%O2、93%N2)培养72 h。采用四氮唑盐(MTT)比色法、结晶紫染色、MilliCell室、流式细胞仪、逆转录-定量聚合酶链反应(RT-qPCR)法、酶联免疫吸附(ELISA)法测定细胞增殖及凋亡水平、单克隆形成数、穿膜数、Nox1 mRNA及P-Akt mRNA表达水平,以及超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)水平。结果B1组、B2组、B3组吸光度(OD)值、存活率水平、克隆形成数、穿膜数、Nox1 mRNA水平、P-Akt mRNA水平、MDA水平明显低于A组(P<0.05),且与剂量呈正相关;B1组、B2组、B3组细胞凋亡率水平、SOD及GSH-Px水平明显高于A组(P<0.05),且与剂量呈正相关。结论紫花前胡素能抑制口腔鳞癌细胞增殖、侵袭,并促进其凋亡,其机制与紫花前胡素抑制Nox1活性降低Akt的磷酸化水平,进而降低其氧化应激水平有关。展开更多
文摘Tisplatin is one of the valuable icancer agents against several types of neoplasm. However, nephrotoxicity is the major adverse effect representing in cisplatin therapy. In this study, the animal tests detecting protective effects of a natural compound, Decursin, on cisplatin-induced nephrotoxicity were examined by using in vivo model. Pretreatment Decursin 10, 20 and 40 mg · kg^-1 at 48, 24 and 6 h, and administration of a single dose of Cisplatin 5.2 mg · kg^-1. Nephrotoxicity was evaluated by serum BUN and creatinine examination. There was significant difference in body weights, serum BUN and creatinine levels of the normal group. Based on the new understanding of the protective mechanisms of cisplatin-induced nephrotocivity, new strategies can be developed to prevent renal injury or to enhance recovery after cisplatin treatment.
文摘The subacute intraperitoneal toxicity of decursin was investigated in Spargue-Dawly（SD） rats. The rats were treated with decursin at a dose of 125 and 250 mg·kg-1·day-1for 4 weeks. All animals were observed daily for clinical signs. Their body weights were recorded weekly, no mortality was observed in two doses of 125 and 250 mg·kg-1of decursin. There were no significant differences in the clinical observations, body weight, food and water consumption, hematology, blood chemistry, gross pathological examination or organ weight between control and treated animals of both sexes. In conclusion, no evidence of a subacute toxic potential was observed in this study and no indication for adverse effects was noted at a dose level of 250 mg·kg-1·day-1.
