摘要
目的建立测定紫花前胡素含量的高效液相色谱方法,检测8种当归属植物中紫花前胡素的含量并比较其差异。方法采用HPLC法,使用AT.LICHROM ODS-C18色谱柱(250×4.6 mm,5μm),以pH 5.0的磷酸-磷酸二氢钠缓冲液(稀释10倍)∶乙腈(1∶1)为流动相,柱温30℃,流速1.0 mL/min,检测波长280 nm。结果紫花前胡素在0.322～165μg/mL范围内线性关系良好(r=0.999 9),加样回收率98.6%,RSD为1.3%。8种当归属植物均含有紫花前胡素,其中吉林产韩国当归含量最高(32.9 mg/g),鸭巴前胡次之(0.992 mg/g),再次为紫花前胡(0.527 mg/g);韩国当归和紫花前胡的花亦富含紫花前胡素。结论 8种当归属植物中紫花前胡素的含量存在差异,且同种植物不同部位也存在差异。该方法简便快捷,准确,适用于当归属及其亲缘属植物中紫花前胡素的定量分析。
Objective To establish a HPLC method for the determination of decursin,determine the content of decursin and compare the differences in eight different Angelica plants.Method The analysis was performed on a AT.LICHROM ODS-C18 column（250 mm×4.6 mm,5 μm） with phosphate-sodium dihydrogen phosphate buffer solvent（diluted 10 times）： acetonitrile（1：1） at pH 5.0 as mobile phase.The flow rate was 1.0 mL/min.The column temperature was 30 ℃ and the detection wavelength was 280 nm.Result The good linearity was in the range of 0.322～165 μg/mL（r=0.999 9）,and the average recovery of the method was 98.6% with the RSD 1.3%.All eight Angelica plants contained decursin.The highest percentage of decursin was in A.gigas Nakai.（32.9 mg/g）,the second in A.decursiva f.albiflora.（0.992 mg/g）,and next in A.peucedanum decursivun（Miq.） Maxim.（0.527 mg/g）.The flowers of A.gigas Nakai.and A.peucedanum decursivun（Miq.） Maxim.were also rich in decursin.Conclusion The content of decursin is different in different Angelica plants and different parts of the same specie.The methods were simple,accurate and reproducible,and can be used for the quantitative analysis of decursin in Angelica plants and their phylogenetic plants.
出处
《湖南中医药大学学报》
CAS
2010年第9期93-95,共3页
Journal of Hunan University of Chinese Medicine
基金
2009年科技部科技型中小企业技术创新基金(09C26224302028)
