[Objective] The aim was to compare the morphology difference between male and female individuals of Paramisgurnus dabryanus Sauvage. [Method] The comparison of 10 morphological characteristics between male and female ...[Objective] The aim was to compare the morphology difference between male and female individuals of Paramisgurnus dabryanus Sauvage. [Method] The comparison of 10 morphological characteristics between male and female individuals of P. dabryanus was conducted with principal component analysis and t-test methods. [Result] The results of t-test indicated that three of 10 morphological characteristics showed extremely significant difference（P0.01）and one of 10 morphological characteristics was significantly different（P0.05）. Principal component analysis revealed four principal components,the contribution rate of which was 31.151%,18.844%,12.324% and 10.169%,respectively. The cumulative contribution rate had reached 72.487%. The morphological variations between male and female individuals were not significant,which was consistent with that of t-test. [Conclusion] The result in this study had provided theoretical basis for the breeding of P. dabryanus.展开更多
Preliminary comparative study was conducted on autumn propagation per- formances of Paramisgumus dabryanus and Misgurnus anguillicaudatus. The count- able and measurable properties, fatness, mature coefficient, relati...Preliminary comparative study was conducted on autumn propagation per- formances of Paramisgumus dabryanus and Misgurnus anguillicaudatus. The count- able and measurable properties, fatness, mature coefficient, relative fecundity, as well as the fertilized and the hatching rates were measured. The results showed that there was no significant difference between the fatness of the two species （P〉0.05） and between the fatness of male and female Paramisgumus dabryanus （P〉0.05）, while significant difference existed between male and female Misgurnus anguillicauda- tus （0.01〈P〈0.05）; the mature coefficients of Paramisgurnus dabryanus and Misgurnus anguillicaudatus were of 6.56% and 11.36%, respectively; the relative fecundities of Paramisgurnus dabryanus and Misgurnus anguillicaudatus were of 115.70 eggs/g and 147.69 eggs/g, respectively; the fertilized rates of Paramisgurnus dabryanus and Mis- gurnus anguillicaudatus were of 90.62% and 97.52%, respectively; and the hatching rates of Paramisgumus dabryanus and Misgurnus anguillicaudatus were of 92.45% and 97.16%, respectively. It was concluded that Misgumus anguillicaudatus was bet- ter than Paramisgurnus dabryanus in breeding performance in autumn.展开更多
The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus(Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power ...The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus(Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power model was estimated for the length-weight relationship during the early life stages. Positive allometric growth for the head segment, trunk length, tail length and eye diameter was also found in the early life stages, while body depth, tail depth, tail fi n length, pectoral fi n length and barbel length displayed a negative coeffi cient. During the subsequent early developmental stage, the growth coeffi cients showed a clear and common tendency towards isometry for all measured body ratios. The allometric growth changes in Chinese loach during the early stage are possibly the result of selective organogenesis directed towards survival priorities.展开更多
Although accumulating data demonstrate that dietary supplementation of bamboo vinegar and charcoal powder(BVC)can significantly strengthen the immunity and boost the growth of domestic animals,its application potency ...Although accumulating data demonstrate that dietary supplementation of bamboo vinegar and charcoal powder(BVC)can significantly strengthen the immunity and boost the growth of domestic animals,its application potency still awaits verification in commercial fish species such as the large-scale loach Paramisgurnus dabryanus.The impacts of 90-day dietary supplementation of 1%and 2%BVC on survival and growth performance of the loach,and on the intestinal morphological characteristics and gut microflora were analyzed.Our data show that the large-scale loach supplied with BVC at the experimental doses had significant higher survival rates and better growth performance(indicated by greater weight gain(1.13–1.14 times),higher specific growth rate(1.04 times),and lower feed conversion ratio(0.88–0.89 times))compared to that of the control(P<0.05).Histological examination revealed significant longer villus(3.22–5.54 times),deeper crypt(1.77–1.87 times),and thicker muscle(1.59–3.17 times)in the intestines of large-scale loach fed with BVC(P<0.05).Furthermore,we found that the gut microflora consisted of significantly fewer proportions of potential pathogenic bacterial species(Aeromonas veronii and Escherichia coli),but significantly greater proportions of beneficial microbes(Lactococus raffinolactis and Faecalibacterium prausnitzii).Therefore,dietary intake of BVC can promote intestinal tract development and optimize gut microflora,by which the survival and growth of large-scale loach may be improved.展开更多
Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus ...Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.展开更多
基金Supported by Scientific and Technological Projects in Shandong Province~~
文摘[Objective] The aim was to compare the morphology difference between male and female individuals of Paramisgurnus dabryanus Sauvage. [Method] The comparison of 10 morphological characteristics between male and female individuals of P. dabryanus was conducted with principal component analysis and t-test methods. [Result] The results of t-test indicated that three of 10 morphological characteristics showed extremely significant difference（P0.01）and one of 10 morphological characteristics was significantly different（P0.05）. Principal component analysis revealed four principal components,the contribution rate of which was 31.151%,18.844%,12.324% and 10.169%,respectively. The cumulative contribution rate had reached 72.487%. The morphological variations between male and female individuals were not significant,which was consistent with that of t-test. [Conclusion] The result in this study had provided theoretical basis for the breeding of P. dabryanus.
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest by the ministry of agriculture(201303056)Patent Technology R&D and Industrialization Demonstration Project of Jiangxi Province(2014BBM26038)~~
文摘Preliminary comparative study was conducted on autumn propagation per- formances of Paramisgumus dabryanus and Misgurnus anguillicaudatus. The count- able and measurable properties, fatness, mature coefficient, relative fecundity, as well as the fertilized and the hatching rates were measured. The results showed that there was no significant difference between the fatness of the two species （P〉0.05） and between the fatness of male and female Paramisgumus dabryanus （P〉0.05）, while significant difference existed between male and female Misgurnus anguillicauda- tus （0.01〈P〈0.05）; the mature coefficients of Paramisgurnus dabryanus and Misgurnus anguillicaudatus were of 6.56% and 11.36%, respectively; the relative fecundities of Paramisgurnus dabryanus and Misgurnus anguillicaudatus were of 115.70 eggs/g and 147.69 eggs/g, respectively; the fertilized rates of Paramisgurnus dabryanus and Mis- gurnus anguillicaudatus were of 90.62% and 97.52%, respectively; and the hatching rates of Paramisgumus dabryanus and Misgurnus anguillicaudatus were of 92.45% and 97.16%, respectively. It was concluded that Misgumus anguillicaudatus was bet- ter than Paramisgurnus dabryanus in breeding performance in autumn.
基金Supported by the National Key Technology Research and Development Program of China(Nos.2012BAD25B08,2012BAD25B00)
文摘The length-weight relationship and allometric growth patterns of hatchery-reared Chinese loach, Paramisgurnus dabryanus(Dabry de Thiersant, 1872), were determined from hatching to 60 days after hatching. A best power model was estimated for the length-weight relationship during the early life stages. Positive allometric growth for the head segment, trunk length, tail length and eye diameter was also found in the early life stages, while body depth, tail depth, tail fi n length, pectoral fi n length and barbel length displayed a negative coeffi cient. During the subsequent early developmental stage, the growth coeffi cients showed a clear and common tendency towards isometry for all measured body ratios. The allometric growth changes in Chinese loach during the early stage are possibly the result of selective organogenesis directed towards survival priorities.
基金Supported by the Key R&D Program of Zhejiang Province(No.2021C02048)the Science and Technology Project of Wenzhou(No.2019ZX002-02)+3 种基金the Natural Science Foundation of Zhejiang Province(No.LQ21C190003)the China Postdoctoral Science Foundation(No.2020M671743)the Zhejiang Provincial Key Laboratory Construction Plans(No.2020E10025)the Open Fund of Key Laboratory of Marine Ecosystem Dynamics,SIO(No.MED2020004)。
文摘Although accumulating data demonstrate that dietary supplementation of bamboo vinegar and charcoal powder(BVC)can significantly strengthen the immunity and boost the growth of domestic animals,its application potency still awaits verification in commercial fish species such as the large-scale loach Paramisgurnus dabryanus.The impacts of 90-day dietary supplementation of 1%and 2%BVC on survival and growth performance of the loach,and on the intestinal morphological characteristics and gut microflora were analyzed.Our data show that the large-scale loach supplied with BVC at the experimental doses had significant higher survival rates and better growth performance(indicated by greater weight gain(1.13–1.14 times),higher specific growth rate(1.04 times),and lower feed conversion ratio(0.88–0.89 times))compared to that of the control(P<0.05).Histological examination revealed significant longer villus(3.22–5.54 times),deeper crypt(1.77–1.87 times),and thicker muscle(1.59–3.17 times)in the intestines of large-scale loach fed with BVC(P<0.05).Furthermore,we found that the gut microflora consisted of significantly fewer proportions of potential pathogenic bacterial species(Aeromonas veronii and Escherichia coli),but significantly greater proportions of beneficial microbes(Lactococus raffinolactis and Faecalibacterium prausnitzii).Therefore,dietary intake of BVC can promote intestinal tract development and optimize gut microflora,by which the survival and growth of large-scale loach may be improved.
文摘Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.
