Bergenin, isolated from the herb of Saxifrage stolonifera Curt.(Hu-Er-Cao) has hepatoprotective, anti-inflammatory, antitussive, and neuroprotective activities. The aim of the present study was to establish a simple, ...Bergenin, isolated from the herb of Saxifrage stolonifera Curt.(Hu-Er-Cao) has hepatoprotective, anti-inflammatory, antitussive, and neuroprotective activities. The aim of the present study was to establish a simple, rapid, and sensitive RP-HPLC method for determination of bergenin in rat plasma and compare its oral pharmacokinetic behaviors in normal and CCl_4-induced hepatic injury rats. With norisoboldine as an internal standard, chromatographic separation was performed on a C_(18) analytical column with acetonitrile and water(11 : 89, V/V) containing 0.1% formic acid as the mobile phase. A good linearity was obtained over the range of 100^(–1)0 000 ng·m L^(–1). The lower limit of quantification was 50 ng·m L~(^(–1)). The developed method was successfully applied to a study of the pharmacokinetic difference of bergenin(100 mg·kg^(–1)) between normal and hepatic injury rats after oral administration. Marked alterations of pharmacokinetic parameters in hepatic injury rats were observed. Compared to normal rats, the AUC_((0–∞)) of bergenin in hepatic injury rats was elevated to 2.11-fold and C_(max) was increased by 130%, whereas CL value was only 55% of the normal rats, suggesting that the systemic exposure of bergenin was significantly increased under hepatic injury status.展开更多
Photosynthetic electron transfer occurs efficiently in specialized internal membranes known as thylakoid membranes.Thylakoid membranes exhibit diverse structural variations across photoautotrophic organisms.We studied...Photosynthetic electron transfer occurs efficiently in specialized internal membranes known as thylakoid membranes.Thylakoid membranes exhibit diverse structural variations across photoautotrophic organisms.We studied how a key protein,CurT,shapes thylakoid membranes of a model cyanobacterium Synechococcus elongatus PCC 7942(Syn7942),a rod-shaped cyanobacterium with regular concentric thylakoid layers.By guiding the curves and structure of thylakoid membranes,CurT helps the cells capture light efficiently,especially when conditions change.The detailed characterization of the role of CurT in Syn7942 offers new clues about how nature builds high-performance photosynthetic membrane systems in response to environmental fluctuations.These findings may inspire future ways to redesign photosynthetic membranes for better crop yields or cleaner bioenergy production.展开更多
以葡萄霜霉病[Plasmopara viticola(Berk et Curt.)Burl.&de Toni]为监测对象,对辽宁省5个葡萄主栽地区的霜霉病流行情况进行了系统监测。结果表明,葡萄霜霉病的发生逐年加重,其中2016年该病害成灾严重。针对葡萄霜霉病流行特点,结...以葡萄霜霉病[Plasmopara viticola(Berk et Curt.)Burl.&de Toni]为监测对象,对辽宁省5个葡萄主栽地区的霜霉病流行情况进行了系统监测。结果表明,葡萄霜霉病的发生逐年加重,其中2016年该病害成灾严重。针对葡萄霜霉病流行特点,结合病害监测情况,分析了未来葡萄霜霉病的发生趋势和防控对策,为该病的流行预测及防治决策提供借鉴参考。展开更多
基金supported by Health Innovation Personnel Training Project of Changzhou City(No.CWKJ 2010-368)Changzhou High Level Health Personnel Training Project
文摘Bergenin, isolated from the herb of Saxifrage stolonifera Curt.(Hu-Er-Cao) has hepatoprotective, anti-inflammatory, antitussive, and neuroprotective activities. The aim of the present study was to establish a simple, rapid, and sensitive RP-HPLC method for determination of bergenin in rat plasma and compare its oral pharmacokinetic behaviors in normal and CCl_4-induced hepatic injury rats. With norisoboldine as an internal standard, chromatographic separation was performed on a C_(18) analytical column with acetonitrile and water(11 : 89, V/V) containing 0.1% formic acid as the mobile phase. A good linearity was obtained over the range of 100^(–1)0 000 ng·m L^(–1). The lower limit of quantification was 50 ng·m L~(^(–1)). The developed method was successfully applied to a study of the pharmacokinetic difference of bergenin(100 mg·kg^(–1)) between normal and hepatic injury rats after oral administration. Marked alterations of pharmacokinetic parameters in hepatic injury rats were observed. Compared to normal rats, the AUC_((0–∞)) of bergenin in hepatic injury rats was elevated to 2.11-fold and C_(max) was increased by 130%, whereas CL value was only 55% of the normal rats, suggesting that the systemic exposure of bergenin was significantly increased under hepatic injury status.
基金supported by the National Key R&D Program of China(2021YFA0909600 and 2023YFA0914600)the National Natural Science Foundation of China(32070109)+1 种基金the Biotechnology and Biological Sciences Research Council(BB/V009729/1,BB/Y01135X/1,and BB/Y008308/1)the Royal Society(URF\R\180030).
文摘Photosynthetic electron transfer occurs efficiently in specialized internal membranes known as thylakoid membranes.Thylakoid membranes exhibit diverse structural variations across photoautotrophic organisms.We studied how a key protein,CurT,shapes thylakoid membranes of a model cyanobacterium Synechococcus elongatus PCC 7942(Syn7942),a rod-shaped cyanobacterium with regular concentric thylakoid layers.By guiding the curves and structure of thylakoid membranes,CurT helps the cells capture light efficiently,especially when conditions change.The detailed characterization of the role of CurT in Syn7942 offers new clues about how nature builds high-performance photosynthetic membrane systems in response to environmental fluctuations.These findings may inspire future ways to redesign photosynthetic membranes for better crop yields or cleaner bioenergy production.
文摘以葡萄霜霉病[Plasmopara viticola(Berk et Curt.)Burl.&de Toni]为监测对象,对辽宁省5个葡萄主栽地区的霜霉病流行情况进行了系统监测。结果表明,葡萄霜霉病的发生逐年加重,其中2016年该病害成灾严重。针对葡萄霜霉病流行特点,结合病害监测情况,分析了未来葡萄霜霉病的发生趋势和防控对策,为该病的流行预测及防治决策提供借鉴参考。
