Autophagy is a cellular process in degradation of long-lived proteins and organelles in the cytosol for maintaining cellular homeostasis,which has been linked to a wide range of human health and disease states,includi...Autophagy is a cellular process in degradation of long-lived proteins and organelles in the cytosol for maintaining cellular homeostasis,which has been linked to a wide range of human health and disease states,including viral infection.The viral infected cells exhibit a compli-cated cross-talking between autophagy and virus.It has been shown that autophagy interacts with both adaptive and innate immunity.For adaptive immunity,viral antigens can be processed in autophagosomes by acidic proteases before major histocompatibility complex(MHC)class II presentation.For innate immunity,autophagy may assist in the delivery of viral nucleic acids to endosomal TLRs and also functions as a part of the TLR-or-PKR-downstream responses.Autophagy was also reported to suppress the magnitude of host innate antiviral immunity in certain cases.On the other hand,viruses has evolved many strategies to combat or utilize the host autophagy for their own benefit.In this review we discussed recent advances toward clarifying the cross-talking between autophagy and viral infection in mammalian cells.展开更多
Background:This study investigated the role of polydatin in regulating macrophage-epithelial cell(EC)interactions during asthma.An asthma model was induced in BALB/c mice using ovalbumin(20μg).Methods:The therapeutic...Background:This study investigated the role of polydatin in regulating macrophage-epithelial cell(EC)interactions during asthma.An asthma model was induced in BALB/c mice using ovalbumin(20μg).Methods:The therapeutic effects of polydatin(20 and 40 mg/kg)were evaluated in this asthmatic mouse model.To assess the underlying mechanisms,Bronchial Epithelium Adenovirus 12-SV402B(BEAS-2B)cells were cocultured with Tohoku Hospital for Pediatrics-1(THP-1)macrophages,in which toll-like receptor 4(TLR4)was either overexpressed or knocked down,and subsequently stimulated with lipopoly-saccharide(LPS)and ATP.THP-1 cells underwent a 1-h pretreatment with polydatin(50 and 100μmol/L),Class Lipid Inhibitor-095(CLI-095,TLR4 inhibitor,1μg/mL),or A438079(P2X7R antagonist,10μmol/L)prior to LPS/ATP challenge.Results:Findings from Western blotting,enzyme-linked immunosorbent assay,flow cytometry,real-time polymerase chain reaction,and immunofluorescence assays demonstrated that modulating TLR4 expression significantly altered interleukin-1β(IL-1β)secretion from THP-1 macrophages and mitochondrial reactive oxygen species(mtROS)production in BEAS-2B ECs.In the mouse asthma model,polydatin significantly alleviated airway inflammation,oxidative stress,and apoptosis,likely by interfering with TLR4/P2X7R-mediated signaling and suppressing the activation of the NOD-like receptor protein inflammasome.Additionally,polydatin significantly reduced IL-1βand IL-18 levels and inhibited the infiltration of macrophages and eosinophils.Correspondingly,polydatin significantly attenuated TLR4/P2X7R signaling in THP-1 cells stimulated with ATP and LPS,thereby reducing IL-1βand IL-18 secretion,calcium influx,mtROS production,and apoptosis in BEAS-2B ECs.Conclusions:Polydatin is a promising therapeutic candidate for asthma,possibly by targeting macrophage-epithelium cross-talk via the TLR4/P2X7R axis.Future formulations as capsules or sprays may effectively alleviate airway inflammation and remodeling.展开更多
Transforming growth factor-beta （TGF-β）/bone morphogenic protein （BMP） signaling is involved in the vast majority of cellular processes and is fundamentally important during the entire life of all metazoans. Dere...Transforming growth factor-beta （TGF-β）/bone morphogenic protein （BMP） signaling is involved in the vast majority of cellular processes and is fundamentally important during the entire life of all metazoans. Deregulation of TGF-β/ BMP activity almost invariably leads to developmental defects and/or diseases, including cancer. The proper functioning of the TGF-β/BMP pathway depends on its constitutive and extensive communication with other signaling pathways, leading to synergistic or antagonistic effects and eventually desirable biological outcomes. The nature of such signaling cross-talk is overwhelmingly complex and highly context-dependent. Here we review the different modes of cross-talk between TGF-β/BMP and the signaling pathways of Mitogen-activated protein kinase, phosphatidylinositol-3 kinase/ Akt, Wnt, Hedgehog, Notch, and the interleukin/interferon-gamma/tumor necrosis factor-alpha cytokines, with an emphasis on the underlying molecular mechanisms.展开更多
Plant development is a complex process influenced by exogenous and endogenous elements.A series of postembryonic developmental events is involved to form the final architecture and contend with the changing environmen...Plant development is a complex process influenced by exogenous and endogenous elements.A series of postembryonic developmental events is involved to form the final architecture and contend with the changing environment.MicroRNA（miRNA） is one of endogenous non-coding RNAs,which plays an important role in plant developmental regulation.In this review,we summarized 34 miRNA families that are closely associated with plant development.Among these families,nine are expressed only in specific organs,whereas 20 families are expressed in at least two different organs.It is known that some miRNAs are expressed across most processes of plant growth,while some appear only at particular developmental stages or under special environmental conditions such as drought,waterlogging and short-day time.These miRNAs execute their diverse functions by regulating developmental gene expression levels,interacting with phytohormone signaling response,participating in the biogenesis of ta-siRNAs and affecting the production of miRNAs.展开更多
The emerging role of gut microbiota as a key player in the development of neurodegenerative disorders:Mammals have evolved together with commensal microbiota to establish a symbiotic relationship in which they regulat...The emerging role of gut microbiota as a key player in the development of neurodegenerative disorders:Mammals have evolved together with commensal microbiota to establish a symbiotic relationship in which they regulate reciprocally by synthesizing and responding to several common chemical substances.In this regard,gut microbiota constitutes a consortium of bacteria that not only participates in the degradation of nutrients,but also produces metabolites,fatty acids and neurotransmitters that can act on the enzymes and receptors expressed in eukaryotic cells,which considerably affects the physiology of the host and contribute to maintaining homeostasis(Lyte,2013).展开更多
Sugars and auxin have important effects on almost all phases of plant life cycle,which are so fundamental to plants and regulate similar processes.However,little is known about the effect of cross-talk between glucose...Sugars and auxin have important effects on almost all phases of plant life cycle,which are so fundamental to plants and regulate similar processes.However,little is known about the effect of cross-talk between glucose and indole-3-acetic acid(IAA)on growth and development of apple trees.To examine the potential roles of glucose and IAA in root architecture,root nitrogen(N)metabolism and photosynthetic capacity in‘Hanfu’(Malus domestica),a total of five treatments was established:single application of glucose,IAA,and auxin polar transport inhibitor(2,3,5-triiodobenzoic acid,TIBA),combined application of glucose with TIBA and that of glucose with IAA.The combined application of glucose with IAA improved root topology system and endogenous IAA content by altering the mRNA levels of several genes involved in root growth,auxin transport and biosynthesis.Moreover,the increased N metabolism enzyme activities and levels of genes expression related to N in roots may suggest higher rates of transformation of nitrate(NO3--N)into amino acids application of glucose and IAA.Contrarily,single application of TIBA decreased the expression levels of auxin transport gene,hindered root growth and decreased endogenous IAA content.Glucose combined with TIBA application effectively attenuated TIBA-induced reductions in root topology structure,photosynthesis and N metabolism activity,and mRNA expression levels involved in auxin biosynthesis and transport.Taken together,glucose application probably changes the expression level of auxin synthesis and transport genes,and induce the allocation of endogenous IAA in root,and thus improves root architecture and N metabolism of root in soil with deficit carbon.展开更多
Today the most important challenge facing the pediatrician is the increasing prevalence of chronic diseases. With this regard, pediatricians play a key role in the management of these conditions. The closeness with th...Today the most important challenge facing the pediatrician is the increasing prevalence of chronic diseases. With this regard, pediatricians play a key role in the management of these conditions. The closeness with the family, the knowledge of the clinical case and the care continuity allow the pediatrician to acquire a position of director of every case. When pathological events have a chronic feature, suddenly the quality of life of the whole family changes. For this reason the first communication of chronic disease is very important and the task of the pediatrician should be to provide a positive message to help the family in facing the difficulty of this new challenge. The bronchial asthma is the most common chronic disease worldwide. The incidence, the prevalence, and the mortality of the disease have increased in children over the past decades. These trends are particularly marked above all in preschool children. The success reached by Pediatricians is closely related to the compliance and the implementation of the therapy followed by the little patient and his family. With this regard authors, in this review, focus on the illustration of several strategies, based on the pediatrician’ skills and medicine documents, that can be used for the improvement of communication among pedia- trician-family and child, never forgetting the hu- man aspect of the same doctor, that should con- ciliate with the scientific knowledge in the taking care of a specific chronic disease.展开更多
The delay fault induced by cross-talk effect is one of the difficult problems in the fault diagnosis of digital circuit. An intelligent fault diagnosis based on IDDT testing and support vector machines （SVM） classif...The delay fault induced by cross-talk effect is one of the difficult problems in the fault diagnosis of digital circuit. An intelligent fault diagnosis based on IDDT testing and support vector machines （SVM） classifier was proposed in this paper. Firstly, the fault model induced by cross-talk effect and the IDDT testing method were analyzed, and then a delay fault localization method based on SVM was presented. The fault features of the sampled signals were extracted by wavelet packet decomposition and served as input parameters of SVM classifier to classify the different fault types. The simulation results illustrate that the method presented is accurate and effective, reaches a high diagnosis rate above 95%.展开更多
Background:The heterogeneity of prognosis and treatment benefits among patients with gliomas is due to tumor microenvironment characteristics.However,biomarkers that reflect microenvironmental characteristics and predic...Background:The heterogeneity of prognosis and treatment benefits among patients with gliomas is due to tumor microenvironment characteristics.However,biomarkers that reflect microenvironmental characteristics and predict the prognosis of gliomas are limited.Therefore,we aimed to develop a model that can effectively predict prognosis,differentiate microenvironment signatures,and optimize drug selection for patients with glioma.Materials and Methods:The CIBERSORT algorithm,bulk sequencing analysis,and single-cell RNA(scRNA)analysis were employed to identify significant cross-talk genes between M2 macrophages and cancer cells in glioma tissues.A predictive model was constructed based on cross-talk gene expression,and its effect on prognosis,recurrence prediction,and microenvironment characteristics was validated in multiple cohorts.The effect of the predictive model on drug selection was evaluated using the OncoPredict algorithm and relevant cellular biology experiments.Results:A high abundance of M2 macrophages in glioma tissues indicates poor prognosis,and cross-talk between macrophages and cancer cells plays a crucial role in shaping the tumor microenvironment.Eight genes involved in the cross-talk between macrophages and cancer cells were identified.Among them,periostin(POSTN),chitinase 3 like 1(CHI3L1),serum amyloid A1(SAA1),and matrix metallopeptidase 9(MMP9)were selected to construct a predictive model.The developed model demonstrated significant efficacy in distinguishing patient prognosis,recurrent cases,and characteristics of high inflammation,hypoxia,and immunosuppression.Furthermore,this model can serve as a valuable tool for guiding the use of trametinib.Conclusions:In summary,this study provides a comprehensive understanding of the interplay between M2 macrophages and cancer cells in glioma;utilizes a cross-talk gene signature to develop a predictive model that can predict the differentiation of patient prognosis,recurrence instances,and microenvironment characteristics;and aids in optimizing the application of trametinib in glioma patients.展开更多
Objective： BCR/ABL oncoprotein-expression is associated with uncontrolled cell growth. Sphingosine kinase 1 （SPK1） regulates the production of sphingosine 1-phosphate （S1P）, a key lipid signal molecular in cell p...Objective： BCR/ABL oncoprotein-expression is associated with uncontrolled cell growth. Sphingosine kinase 1 （SPK1） regulates the production of sphingosine 1-phosphate （S1P）, a key lipid signal molecular in cell proliferation and survival. The objective of this study was to elucidate the roles of S1P and its receptors in bcr/abl positive chronic myeloid leukemia （CML） cells. Methods： The expressions of SIP receptors： S1P1, S1P2 and S1P3 in CML cells were detected by RT-PCR. SPK1 expression, activity and extracellular S1P were determined in ECV304 and HL-60 cells which were transfected with bcr/abl gene. To elucidate the relationship between the BCR/ABL, ERK/MAPK （extracellular signal-regulated kinase/mitogen-activated protein kinase）, SPK/S 1P and S 1P/S 1 P2 signal pathways, bcr/abl positive CML cell line K562 was treated with STI571, PD98059, N,N-dimethyl sphingosine （DMS） and JTE-013. Results： Retrovirus-mediated overexpression of bcr/abl gene in ECV304 and HL-60 cells resulted in upregulation of the expression, activity of SPK1 and increase of the secretion of SIP, whereas treatment of STI571 and PD98059 decreased the BCR/ABL-induced S1P secretion. Treatment of DMS reduced S1P secretion and P42/44MAPK phosphorylation. S1P2-selective antagonist JTE-013 could also decrease P42/44MAPK phosphorylation. Conclusion： These results suggest that BCR/ABL up-regulates extracellular sphingosine 1-phosphate through sphingosine kinase 1 and there is cross-talk between SPK1/S1P/S1P2 and P42/44MAPK in bcr/abl positive CML cells.展开更多
Blind source separation is a signal processing method based on independent component analysis, its aim is to separate the source signals from a set of observations (output of sensors) by assuming the source signals in...Blind source separation is a signal processing method based on independent component analysis, its aim is to separate the source signals from a set of observations (output of sensors) by assuming the source signals independently. This paper reviews the general concept of BSS firstly;especially the theory for convolutive mixtures, the model of convolutive mixture and two deconvolution structures, then adopts a BSS algorithm for convolutive mixtures based on residual cross-talking error threshold control criteria, the simulation testing points out good performance for simulated mixtures.展开更多
The elaborate redox network of the cell,comprising of events like turnover of reactive oxygen species(ROS),redox sensing,signaling,expression of redox-sensitive genes,etc.,often orchestrates with other bonafide hormon...The elaborate redox network of the cell,comprising of events like turnover of reactive oxygen species(ROS),redox sensing,signaling,expression of redox-sensitive genes,etc.,often orchestrates with other bonafide hormonal signaling pathways through their synergistic or antagonistic action in the plant cell.The redox cue generated in plant cells under fluctuating environmental conditions can significantly influence other hormonal biosynthetic or signaling mechanisms,thereby modulating physiology towards stress acclimation and defense.There is also strong evidence of the recruitment of ROS as a‘second messenger’in different hormonal signaling pathways under stress.Moreover,the retrograde signaling initiated by ROS also found to strongly influence hormonal homeostasis and signaling.The present review,in this aspect,is an effort towards understanding the regulatory roles of ROS in integrating and orchestrating other hormonal signaling pathways or vice versa so as to unfold the relationship between these two signaling episodes of plant cells under environmental odds.We also accentuate the significance of understanding the utterly complex interactions,which occur both at metabolic and genetic levels between ROS and phytohormones during stress combinations.Furthermore,the significant and decisive role of ROS turnover,particularly the contribution of RBOH(respiratory burst oxidase homologs)in the synergism of redox and hormone signaling during systemic acquired acclimation under stress is also discussed.展开更多
MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPI(families have been characterized: extracellular si...MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPI(families have been characterized: extracellular signal-regulated kinase (ERK), Jun kinase (JNK/SAPK) and p38 MAPK. The above effects are fulfilled by regulation of cell cycle engine and other cell proliferation related proteins. In this paper we discussed their functions and cooperation with other signal pathways in regulation of cell proliferation.展开更多
基金supported by grants from the National Natural Science Foundation of China(Grant No.30700696).
文摘Autophagy is a cellular process in degradation of long-lived proteins and organelles in the cytosol for maintaining cellular homeostasis,which has been linked to a wide range of human health and disease states,including viral infection.The viral infected cells exhibit a compli-cated cross-talking between autophagy and virus.It has been shown that autophagy interacts with both adaptive and innate immunity.For adaptive immunity,viral antigens can be processed in autophagosomes by acidic proteases before major histocompatibility complex(MHC)class II presentation.For innate immunity,autophagy may assist in the delivery of viral nucleic acids to endosomal TLRs and also functions as a part of the TLR-or-PKR-downstream responses.Autophagy was also reported to suppress the magnitude of host innate antiviral immunity in certain cases.On the other hand,viruses has evolved many strategies to combat or utilize the host autophagy for their own benefit.In this review we discussed recent advances toward clarifying the cross-talking between autophagy and viral infection in mammalian cells.
基金National Natural Science Foundation of China,Grant/Award Number:82260007Jilin Province Health Commission,Grant/Award Number:2024A062+1 种基金Jilin Provincial Department of Education,Grant/Award Number:JJKH20240698KJJilin Province Science and Technology Department,Grant/Award Number:20240404025ZP and 20240602100RC。
文摘Background:This study investigated the role of polydatin in regulating macrophage-epithelial cell(EC)interactions during asthma.An asthma model was induced in BALB/c mice using ovalbumin(20μg).Methods:The therapeutic effects of polydatin(20 and 40 mg/kg)were evaluated in this asthmatic mouse model.To assess the underlying mechanisms,Bronchial Epithelium Adenovirus 12-SV402B(BEAS-2B)cells were cocultured with Tohoku Hospital for Pediatrics-1(THP-1)macrophages,in which toll-like receptor 4(TLR4)was either overexpressed or knocked down,and subsequently stimulated with lipopoly-saccharide(LPS)and ATP.THP-1 cells underwent a 1-h pretreatment with polydatin(50 and 100μmol/L),Class Lipid Inhibitor-095(CLI-095,TLR4 inhibitor,1μg/mL),or A438079(P2X7R antagonist,10μmol/L)prior to LPS/ATP challenge.Results:Findings from Western blotting,enzyme-linked immunosorbent assay,flow cytometry,real-time polymerase chain reaction,and immunofluorescence assays demonstrated that modulating TLR4 expression significantly altered interleukin-1β(IL-1β)secretion from THP-1 macrophages and mitochondrial reactive oxygen species(mtROS)production in BEAS-2B ECs.In the mouse asthma model,polydatin significantly alleviated airway inflammation,oxidative stress,and apoptosis,likely by interfering with TLR4/P2X7R-mediated signaling and suppressing the activation of the NOD-like receptor protein inflammasome.Additionally,polydatin significantly reduced IL-1βand IL-18 levels and inhibited the infiltration of macrophages and eosinophils.Correspondingly,polydatin significantly attenuated TLR4/P2X7R signaling in THP-1 cells stimulated with ATP and LPS,thereby reducing IL-1βand IL-18 secretion,calcium influx,mtROS production,and apoptosis in BEAS-2B ECs.Conclusions:Polydatin is a promising therapeutic candidate for asthma,possibly by targeting macrophage-epithelium cross-talk via the TLR4/P2X7R axis.Future formulations as capsules or sprays may effectively alleviate airway inflammation and remodeling.
文摘Transforming growth factor-beta （TGF-β）/bone morphogenic protein （BMP） signaling is involved in the vast majority of cellular processes and is fundamentally important during the entire life of all metazoans. Deregulation of TGF-β/ BMP activity almost invariably leads to developmental defects and/or diseases, including cancer. The proper functioning of the TGF-β/BMP pathway depends on its constitutive and extensive communication with other signaling pathways, leading to synergistic or antagonistic effects and eventually desirable biological outcomes. The nature of such signaling cross-talk is overwhelmingly complex and highly context-dependent. Here we review the different modes of cross-talk between TGF-β/BMP and the signaling pathways of Mitogen-activated protein kinase, phosphatidylinositol-3 kinase/ Akt, Wnt, Hedgehog, Notch, and the interleukin/interferon-gamma/tumor necrosis factor-alpha cytokines, with an emphasis on the underlying molecular mechanisms.
基金partially supported by the grants from National Natural Science Foundation of China (NSFC)(No.30971743)the National High Technology Research and Development Program of China(863 Program) (No.2008AA10Z125)the Fundamental Research Funds for the Central Universities
文摘Plant development is a complex process influenced by exogenous and endogenous elements.A series of postembryonic developmental events is involved to form the final architecture and contend with the changing environment.MicroRNA（miRNA） is one of endogenous non-coding RNAs,which plays an important role in plant developmental regulation.In this review,we summarized 34 miRNA families that are closely associated with plant development.Among these families,nine are expressed only in specific organs,whereas 20 families are expressed in at least two different organs.It is known that some miRNAs are expressed across most processes of plant growth,while some appear only at particular developmental stages or under special environmental conditions such as drought,waterlogging and short-day time.These miRNAs execute their diverse functions by regulating developmental gene expression levels,interacting with phytohormone signaling response,participating in the biogenesis of ta-siRNAs and affecting the production of miRNAs.
基金supported by Programa de Apoyo a Centros con Financiamiento Basal AFB-170004(to Fundación Ciencia&Vida)from“Comisión Nacional de Investigación Científica y Tecnológica de Chile(CONICYT)”by grants FONDECYT-1170093 from Fondo Nacional de Desarrollo Científico y Tecnológico de ChileMJFF-10332.01 and MJFF-15076 from Michael J.Fox Foundation for Parkinson Research
文摘The emerging role of gut microbiota as a key player in the development of neurodegenerative disorders:Mammals have evolved together with commensal microbiota to establish a symbiotic relationship in which they regulate reciprocally by synthesizing and responding to several common chemical substances.In this regard,gut microbiota constitutes a consortium of bacteria that not only participates in the degradation of nutrients,but also produces metabolites,fatty acids and neurotransmitters that can act on the enzymes and receptors expressed in eukaryotic cells,which considerably affects the physiology of the host and contribute to maintaining homeostasis(Lyte,2013).
基金supported by the National Key Research and Development Program of China(Grant No.2020YFD1000201)National Natural Science Foundation(Grant No.31972359)+1 种基金Earmarked Fund for CARS(Grant No.CARS-27)Agricultural Research and Industrialization Project of Liaoning Province(Grant No.2020JH2/10200028).
文摘Sugars and auxin have important effects on almost all phases of plant life cycle,which are so fundamental to plants and regulate similar processes.However,little is known about the effect of cross-talk between glucose and indole-3-acetic acid(IAA)on growth and development of apple trees.To examine the potential roles of glucose and IAA in root architecture,root nitrogen(N)metabolism and photosynthetic capacity in‘Hanfu’(Malus domestica),a total of five treatments was established:single application of glucose,IAA,and auxin polar transport inhibitor(2,3,5-triiodobenzoic acid,TIBA),combined application of glucose with TIBA and that of glucose with IAA.The combined application of glucose with IAA improved root topology system and endogenous IAA content by altering the mRNA levels of several genes involved in root growth,auxin transport and biosynthesis.Moreover,the increased N metabolism enzyme activities and levels of genes expression related to N in roots may suggest higher rates of transformation of nitrate(NO3--N)into amino acids application of glucose and IAA.Contrarily,single application of TIBA decreased the expression levels of auxin transport gene,hindered root growth and decreased endogenous IAA content.Glucose combined with TIBA application effectively attenuated TIBA-induced reductions in root topology structure,photosynthesis and N metabolism activity,and mRNA expression levels involved in auxin biosynthesis and transport.Taken together,glucose application probably changes the expression level of auxin synthesis and transport genes,and induce the allocation of endogenous IAA in root,and thus improves root architecture and N metabolism of root in soil with deficit carbon.
文摘Today the most important challenge facing the pediatrician is the increasing prevalence of chronic diseases. With this regard, pediatricians play a key role in the management of these conditions. The closeness with the family, the knowledge of the clinical case and the care continuity allow the pediatrician to acquire a position of director of every case. When pathological events have a chronic feature, suddenly the quality of life of the whole family changes. For this reason the first communication of chronic disease is very important and the task of the pediatrician should be to provide a positive message to help the family in facing the difficulty of this new challenge. The bronchial asthma is the most common chronic disease worldwide. The incidence, the prevalence, and the mortality of the disease have increased in children over the past decades. These trends are particularly marked above all in preschool children. The success reached by Pediatricians is closely related to the compliance and the implementation of the therapy followed by the little patient and his family. With this regard authors, in this review, focus on the illustration of several strategies, based on the pediatrician’ skills and medicine documents, that can be used for the improvement of communication among pedia- trician-family and child, never forgetting the hu- man aspect of the same doctor, that should con- ciliate with the scientific knowledge in the taking care of a specific chronic disease.
基金Supported by the National Natural Science Foun-dation of China (60374008 ,60501022)
文摘The delay fault induced by cross-talk effect is one of the difficult problems in the fault diagnosis of digital circuit. An intelligent fault diagnosis based on IDDT testing and support vector machines （SVM） classifier was proposed in this paper. Firstly, the fault model induced by cross-talk effect and the IDDT testing method were analyzed, and then a delay fault localization method based on SVM was presented. The fault features of the sampled signals were extracted by wavelet packet decomposition and served as input parameters of SVM classifier to classify the different fault types. The simulation results illustrate that the method presented is accurate and effective, reaches a high diagnosis rate above 95%.
基金funded by the Scientific Research Project of the Higher Education Department of Guizhou Province[Qianjiaoji 2022(187)]Department of Education of Guizhou Province[Guizhou Teaching and Technology(2023)015]+1 种基金Guizhou Medical University National Natural Science Foundation Cultivation Project(22NSFCP45)China Postdoctoral Science Foundation Project(General Program No.2022M720929).
文摘Background:The heterogeneity of prognosis and treatment benefits among patients with gliomas is due to tumor microenvironment characteristics.However,biomarkers that reflect microenvironmental characteristics and predict the prognosis of gliomas are limited.Therefore,we aimed to develop a model that can effectively predict prognosis,differentiate microenvironment signatures,and optimize drug selection for patients with glioma.Materials and Methods:The CIBERSORT algorithm,bulk sequencing analysis,and single-cell RNA(scRNA)analysis were employed to identify significant cross-talk genes between M2 macrophages and cancer cells in glioma tissues.A predictive model was constructed based on cross-talk gene expression,and its effect on prognosis,recurrence prediction,and microenvironment characteristics was validated in multiple cohorts.The effect of the predictive model on drug selection was evaluated using the OncoPredict algorithm and relevant cellular biology experiments.Results:A high abundance of M2 macrophages in glioma tissues indicates poor prognosis,and cross-talk between macrophages and cancer cells plays a crucial role in shaping the tumor microenvironment.Eight genes involved in the cross-talk between macrophages and cancer cells were identified.Among them,periostin(POSTN),chitinase 3 like 1(CHI3L1),serum amyloid A1(SAA1),and matrix metallopeptidase 9(MMP9)were selected to construct a predictive model.The developed model demonstrated significant efficacy in distinguishing patient prognosis,recurrent cases,and characteristics of high inflammation,hypoxia,and immunosuppression.Furthermore,this model can serve as a valuable tool for guiding the use of trametinib.Conclusions:In summary,this study provides a comprehensive understanding of the interplay between M2 macrophages and cancer cells in glioma;utilizes a cross-talk gene signature to develop a predictive model that can predict the differentiation of patient prognosis,recurrence instances,and microenvironment characteristics;and aids in optimizing the application of trametinib in glioma patients.
基金supported by the National Natural Science Foundation of China (No. 30570782).
文摘Objective： BCR/ABL oncoprotein-expression is associated with uncontrolled cell growth. Sphingosine kinase 1 （SPK1） regulates the production of sphingosine 1-phosphate （S1P）, a key lipid signal molecular in cell proliferation and survival. The objective of this study was to elucidate the roles of S1P and its receptors in bcr/abl positive chronic myeloid leukemia （CML） cells. Methods： The expressions of SIP receptors： S1P1, S1P2 and S1P3 in CML cells were detected by RT-PCR. SPK1 expression, activity and extracellular S1P were determined in ECV304 and HL-60 cells which were transfected with bcr/abl gene. To elucidate the relationship between the BCR/ABL, ERK/MAPK （extracellular signal-regulated kinase/mitogen-activated protein kinase）, SPK/S 1P and S 1P/S 1 P2 signal pathways, bcr/abl positive CML cell line K562 was treated with STI571, PD98059, N,N-dimethyl sphingosine （DMS） and JTE-013. Results： Retrovirus-mediated overexpression of bcr/abl gene in ECV304 and HL-60 cells resulted in upregulation of the expression, activity of SPK1 and increase of the secretion of SIP, whereas treatment of STI571 and PD98059 decreased the BCR/ABL-induced S1P secretion. Treatment of DMS reduced S1P secretion and P42/44MAPK phosphorylation. S1P2-selective antagonist JTE-013 could also decrease P42/44MAPK phosphorylation. Conclusion： These results suggest that BCR/ABL up-regulates extracellular sphingosine 1-phosphate through sphingosine kinase 1 and there is cross-talk between SPK1/S1P/S1P2 and P42/44MAPK in bcr/abl positive CML cells.
文摘Blind source separation is a signal processing method based on independent component analysis, its aim is to separate the source signals from a set of observations (output of sensors) by assuming the source signals independently. This paper reviews the general concept of BSS firstly;especially the theory for convolutive mixtures, the model of convolutive mixture and two deconvolution structures, then adopts a BSS algorithm for convolutive mixtures based on residual cross-talking error threshold control criteria, the simulation testing points out good performance for simulated mixtures.
基金DST-SERB(Government of India)for research funding(No.CRG/2021/000513,dated 15/12/2021)UGC-CAS and DST-FIST(Government of India)for infrastructural support for research to the Department of Botany,University of Burdwan,India[No.F.5-13/012(SAP-II),and No.SRFST/LSI/2018/188(C)]+4 种基金the University Grants Commission(UGC),New Delhi,for Junior Research Fellowship(Joint CSIR-UGC)the State Funded Research Grant,Government of West Bengal.India[No.FC(Sc.)/RS/SF/BOT/2016-17/210/1(4)]Department of Science Technology and Biotechnology(DSTBT),Government of West Bengal.IndiaIndian Council for Cultural Relations(ICCR)for India Scholarships(Bangladesh)Scheme,2016-2017(No.DAC/EDU/17/1/2016,dated 10.07.2016)DST-SERB,Government of India.
文摘The elaborate redox network of the cell,comprising of events like turnover of reactive oxygen species(ROS),redox sensing,signaling,expression of redox-sensitive genes,etc.,often orchestrates with other bonafide hormonal signaling pathways through their synergistic or antagonistic action in the plant cell.The redox cue generated in plant cells under fluctuating environmental conditions can significantly influence other hormonal biosynthetic or signaling mechanisms,thereby modulating physiology towards stress acclimation and defense.There is also strong evidence of the recruitment of ROS as a‘second messenger’in different hormonal signaling pathways under stress.Moreover,the retrograde signaling initiated by ROS also found to strongly influence hormonal homeostasis and signaling.The present review,in this aspect,is an effort towards understanding the regulatory roles of ROS in integrating and orchestrating other hormonal signaling pathways or vice versa so as to unfold the relationship between these two signaling episodes of plant cells under environmental odds.We also accentuate the significance of understanding the utterly complex interactions,which occur both at metabolic and genetic levels between ROS and phytohormones during stress combinations.Furthermore,the significant and decisive role of ROS turnover,particularly the contribution of RBOH(respiratory burst oxidase homologs)in the synergism of redox and hormone signaling during systemic acquired acclimation under stress is also discussed.
基金supported by the Major StateBasic Research Project (Grant No. G1999053901).
文摘MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPI(families have been characterized: extracellular signal-regulated kinase (ERK), Jun kinase (JNK/SAPK) and p38 MAPK. The above effects are fulfilled by regulation of cell cycle engine and other cell proliferation related proteins. In this paper we discussed their functions and cooperation with other signal pathways in regulation of cell proliferation.
