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Deep Learning Based Efficient Crowd Counting System
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作者 Waleed Khalid Al-Ghanem Emad Ul Haq Qazi +1 位作者 Muhammad Hamza Faheem Syed Shah Amanullah Quadri 《Computers, Materials & Continua》 SCIE EI 2024年第6期4001-4020,共20页
Estimation of crowd count is becoming crucial nowadays,as it can help in security surveillance,crowd monitoring,and management for different events.It is challenging to determine the approximate crowd size from an ima... Estimation of crowd count is becoming crucial nowadays,as it can help in security surveillance,crowd monitoring,and management for different events.It is challenging to determine the approximate crowd size from an image of the crowd’s density.Therefore in this research study,we proposed a multi-headed convolutional neural network architecture-based model for crowd counting,where we divided our proposed model into two main components:(i)the convolutional neural network,which extracts the feature across the whole image that is given to it as an input,and(ii)the multi-headed layers,which make it easier to evaluate density maps to estimate the number of people in the input image and determine their number in the crowd.We employed the available public benchmark crowd-counting datasets UCF CC 50 and ShanghaiTech parts A and B for model training and testing to validate the model’s performance.To analyze the results,we used two metrics Mean Absolute Error(MAE)and Mean Square Error(MSE),and compared the results of the proposed systems with the state-of-art models of crowd counting.The results show the superiority of the proposed system. 展开更多
关键词 Crowd counting EfficientNet multi-head attention convolutional neural network transfer learning
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A Multi-Stage Pipeline for Date Fruit Processing: Integrating YOLOv11 Detection, Classification, and Automated Counting
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作者 Ali S.Alzaharani Abid Iqbal 《Computers, Materials & Continua》 2026年第1期1327-1353,共27页
In this study,an automated multimodal system for detecting,classifying,and dating fruit was developed using a two-stage YOLOv11 pipeline.In the first stage,the YOLOv11 detection model locates individual date fruits in... In this study,an automated multimodal system for detecting,classifying,and dating fruit was developed using a two-stage YOLOv11 pipeline.In the first stage,the YOLOv11 detection model locates individual date fruits in real time by drawing bounding boxes around them.These bounding boxes are subsequently passed to a YOLOv11 classification model,which analyzes cropped images and assigns class labels.An additional counting module automatically tallies the detected fruits,offering a near-instantaneous estimation of quantity.The experimental results suggest high precision and recall for detection,high classification accuracy(across 15 classes),and near-perfect counting in real time.This paper presents a multi-stage pipeline for date fruit detection,classification,and automated counting,employing YOLOv11-based models to achieve high accuracy while maintaining real-time throughput.The results demonstrated that the detection precision exceeded 90%,the classification accuracy approached 92%,and the counting module correlated closely with the manual tallies.These findings confirm the potential of reducing manual labour and enhancing operational efficiency in post-harvesting processes.Future studies will include dataset expansion,user-centric interfaces,and integration with harvesting robotics. 展开更多
关键词 Date fruit cultivation YOLOv11 precision agriculture real-time processing automated fruit counting deep learning agricultural productivity
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HUANNet: A High-Resolution Unified Attention Network for Accurate Counting
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作者 Haixia Wang Huan Zhang +2 位作者 Xiuling Wang Xule Xin Zhiguo Zhang 《Computers, Materials & Continua》 2026年第1期1722-1741,共20页
Accurately counting dense objects in complex and diverse backgrounds is a significant challenge in computer vision,with applications ranging from crowd counting to various other object counting tasks.To address this,w... Accurately counting dense objects in complex and diverse backgrounds is a significant challenge in computer vision,with applications ranging from crowd counting to various other object counting tasks.To address this,we propose HUANNet(High-Resolution Unified Attention Network),a convolutional neural network designed to capture both local features and rich semantic information through a high-resolution representation learning framework,while optimizing computational distribution across parallel branches.HUANNet introduces three core modules:the High-Resolution Attention Module(HRAM),which enhances feature extraction by optimizing multiresolution feature fusion;the Unified Multi-Scale Attention Module(UMAM),which integrates spatial,channel,and convolutional kernel information through an attention mechanism applied across multiple levels of the network;and the Grid-Assisted Point Matching Module(GPMM),which stabilizes and improves point-to-point matching by leveraging grid-based mechanisms.Extensive experiments show that HUANNet achieves competitive results on the ShanghaiTech Part A/B crowd counting datasets and sets new state-of-the-art performance on dense object counting datasets such as CARPK and XRAY-IECCD,demonstrating the effectiveness and versatility of HUANNet. 展开更多
关键词 Accurate counting high-resolution representations point-to-point matching
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Identification of important factors influencing nonlinear counting systems
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作者 Xinmin ZHANG Jingbo WANG +1 位作者 Chihang WEI Zhihuan SONG 《Frontiers of Information Technology & Electronic Engineering》 SCIE EI CSCD 2022年第1期123-133,共11页
Identifying factors that exert more influence on system output from data is one of the most challenging tasks in science and engineering.In this work,a sensitivity analysis of the generalized Gaussian process regressi... Identifying factors that exert more influence on system output from data is one of the most challenging tasks in science and engineering.In this work,a sensitivity analysis of the generalized Gaussian process regression(SA-GGPR)model is proposed to identify important factors of the nonlinear counting system.In SA-GGPR,the GGPR model with Poisson likelihood is adopted to describe the nonlinear counting system.The GGPR model with Poisson likelihood inherits the merits of nonparametric kernel learning and Poisson distribution,and can handle complex nonlinear counting systems.Nevertheless,understanding the relationships between model inputs and output in the GGPR model with Poisson likelihood is not readily accessible due to its nonparametric and kernel structure.SA-GGPR addresses this issue by providing a quantitative assessment of how different inputs affect the system output.The application results on a simulated nonlinear counting system and a real steel casting-rolling process have demonstrated that the proposed SA-GGPR method outperforms several state-of-the-art methods in identification accuracy. 展开更多
关键词 Important factors Nonlinear counting system Generalized Gaussian process regression Sensitivity analysis Steel casting-rolling process
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Boundary evaluation and error correction on pseudorandom spread spectrum photon counting system
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作者 Shanshan Shen Qian Chen +1 位作者 Weiji He Vuqiang Wang 《Chinese Optics Letters》 SCIE EI CAS CSCD 2017年第9期36-40,共5页
The Cramer–Rao lower bound on range error is modeled for pseudo-random ranging systems using Geiger-mode avalanche photodiodes. The theoretical results are shown to agree with the Monte Carlo simulation, satisfying b... The Cramer–Rao lower bound on range error is modeled for pseudo-random ranging systems using Geiger-mode avalanche photodiodes. The theoretical results are shown to agree with the Monte Carlo simulation, satisfying boundary evaluations. Experimental tests prove that range errors caused by the fluctuation of the number of photon counts in the laser echo pulse leads to the range drift of the time point spread function. The function relationship between the range error and the photon counting ratio is determined by using numerical fitting.Range errors due to a different echo energy is calibrated so that the corrected range root mean square error is improved to 1 cm. 展开更多
关键词 Boundary evaluation and error correction on pseudorandom spread spectrum photon counting system
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Development of automatic counting system for urediospores of wheat stripe rust based on image processing 被引量:6
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作者 Li Xiaolong Ma Zhanhong +3 位作者 Fernando Bienvenido Qin Feng Wang Haiguang José Antonio Alvarez-Bermejo 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2017年第5期134-143,共10页
To realize automatic counting of urediospores of Puccinia striiformis f.sp.tritici(Pst)(causal agent of wheat stripe rust),an automatic counting system for urediospores of wheat stripe rust pathogen based on image pro... To realize automatic counting of urediospores of Puccinia striiformis f.sp.tritici(Pst)(causal agent of wheat stripe rust),an automatic counting system for urediospores of wheat stripe rust pathogen based on image processing was developed using MATLAB GUIDE platform in combination with Local C Compiler(LCC).The system is independent of the MATLAB environment and can be run on a computer without the MATLAB software.Using this system,automatic counting of Pst urediospores in a microscopic image can be implemented via image processing technologies including image scaling,clustering segmentation,morphological modification,watershed transformation,connected region labeling,etc.Structure design of the automatic counting system,the key algorithms used in the system and realization of the main functions of the system were described in detail.Spore counting tests were conducted using microscopic digital images of Pst urediospores and the high accuracies more than 95%were obtained.The results indicated that it is feasible to count Pst urediospores automatically using the developed system based on image processing. 展开更多
关键词 puccinia striiformis f.sp.tritici wheat stripe rust image processing automatic counting computer aided system MATLAB
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Photon-counting chirped amplitude modulation lidar system using superconducting nanowire single-photon detector at 1550-nm wavelength 被引量:1
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作者 周慧 何宇昊 +8 位作者 吕超林 尤立星 李召辉 吴光 张伟君 张露 刘晓宇 杨晓燕 王镇 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第1期593-597,共5页
We demonstrate a photon-counting chirped amplitude modulation (CAM) light detection and ranging (lidar) system incorporating a superconducting nanowire single-photon detector (SNSPD) and operated at a wavelength... We demonstrate a photon-counting chirped amplitude modulation (CAM) light detection and ranging (lidar) system incorporating a superconducting nanowire single-photon detector (SNSPD) and operated at a wavelength of 1550 nm. The distance accuracy of the lidar system was determined by the CAM bandwidth and signal-to-noise ratio (SNR) of an intermediate frequency (IF) signal. Owing to a short dead time (10 ns) and negligible dark count rate (70 Hz) of the SNSPD, the obtained IF signal attained an SNR of 42 dB and the direct distance accuracy was improved to 3 mm when the modulation bandwidth of the CAM signal was 240 MHz and the modulation period was 1 ms. 展开更多
关键词 photon counting PHOTODETECTORS LIDAR
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Uncertainty of an automatic system for counting alpha tracks on CR-39
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作者 Dun-Huang Fan Wei-Hai Zhuo +1 位作者 Bo Chen Wei-Yuan Zhang 《Nuclear Science and Techniques》 SCIE CAS CSCD 2017年第11期236-241,共6页
For accurate counting of alpha tracks on the polyallyl diglycol carbonate of CR-39-type track detectors,the size distributions of both artifact tracks and alpha tracks were investigated with an automatic counting syst... For accurate counting of alpha tracks on the polyallyl diglycol carbonate of CR-39-type track detectors,the size distributions of both artifact tracks and alpha tracks were investigated with an automatic counting system. At the same temperature and etchant concentration, the numbers and sizes of alpha tracks changed significantly with the etching time, and the artifact track changes were smaller. At the etching time of 5 h, the sizes of alpha tracks were evidently larger than those of the artifact tracks, and the deviation of its size distribution was much smaller than those of longer etching time. Based on the size distribution of alpha tracks etched for 5 h, the overlap effect and uncertainty of overlap correction were studied by the Monte Carlo simulations for different track densities. It was found that the counting uncertainty of the system could be less than 6% in a density range of 10–160 tracks mm^(-2) after taking the overlap correction into account. 展开更多
关键词 UNCERTAINTY Track counting CR-39 ETCHING OVERLAP
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A Deep Learning-Based Crowd Counting Method and System Implementation on Neural Processing Unit Platform
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作者 Yuxuan Gu Meng Wu +2 位作者 Qian Wang Siguang Chen Lijun Yang 《Computers, Materials & Continua》 SCIE EI 2023年第4期493-512,共20页
In this paper, a deep learning-based method is proposed for crowdcountingproblems. Specifically, by utilizing the convolution kernel densitymap, the ground truth is generated dynamically to enhance the featureextracti... In this paper, a deep learning-based method is proposed for crowdcountingproblems. Specifically, by utilizing the convolution kernel densitymap, the ground truth is generated dynamically to enhance the featureextractingability of the generator model. Meanwhile, the “cross stage partial”module is integrated into congested scene recognition network (CSRNet) toobtain a lightweight network model. In addition, to compensate for the accuracydrop owing to the lightweight model, we take advantage of “structuredknowledge transfer” to train the model in an end-to-end manner. It aimsto accelerate the fitting speed and enhance the learning ability of the studentmodel. The crowd-counting system solution for edge computing is alsoproposed and implemented on an embedded device equipped with a neuralprocessing unit. Simulations demonstrate the performance improvement ofthe proposed solution in terms of model size, processing speed and accuracy.The performance on the Venice dataset shows that the mean absolute error(MAE) and the root mean squared error (RMSE) of our model drop by32.63% and 39.18% compared with CSRNet. Meanwhile, the performance onthe ShanghaiTech PartB dataset reveals that the MAE and the RMSE of ourmodel are close to those of CSRNet. Therefore, we provide a novel embeddedplatform system scheme for public safety pre-warning applications. 展开更多
关键词 Crowd counting CSRNet dynamic density map lightweight model knowledge transfer
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Photon counting statistics of V-type three-level systems:The effects of the field fluctuations
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作者 彭勇刚 郑雨军 《Chinese Physics B》 SCIE EI CAS CSCD 2015年第2期152-157,共6页
We investigate the influence of the field fluctuations to the emission photons of V-type three-level systems.The emission intensity I and Mandel's Q parameter show stochastic resonance with respect to the pure dephas... We investigate the influence of the field fluctuations to the emission photons of V-type three-level systems.The emission intensity I and Mandel's Q parameter show stochastic resonance with respect to the pure dephasing constantγp.The amplitude fluctuation of the field causes these systems to lose their coherence.On the other hand,the amplitude fluctuation provides a new interference method for these systems.The quantum beats are shown in the orthogonal system. 展开更多
关键词 photon counting statistics field fluctuation V-type three-level system
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A Computer Vision-Based System for Metal Sheet Pick Counting
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作者 Jirasak Ji Warut Pannakkong Jirachai Buddhakulsomsiri 《Computers, Materials & Continua》 SCIE EI 2023年第5期3643-3656,共14页
Inventory counting is crucial to manufacturing industries in terms of inventory management,production,and procurement planning.Many companies currently require workers to manually count and track the status of materia... Inventory counting is crucial to manufacturing industries in terms of inventory management,production,and procurement planning.Many companies currently require workers to manually count and track the status of materials,which are repetitive and non-value-added activities but incur significant costs to the companies as well as mental fatigue to the employees.This research aims to develop a computer vision system that can automate the material counting activity without applying any marker on the material.The type of material of interest is metal sheet,whose shape is simple,a large rectangular shape,yet difficult to detect.The use of computer vision technology can reduce the costs incurred fromthe loss of high-value materials,eliminate repetitive work requirements for skilled labor,and reduce human error.A computer vision system is proposed and tested on a metal sheet picking process formultiple metal sheet stacks in the storage area by using one video camera.Our results show that the proposed computer vision system can count the metal sheet picks under a real situation with a precision of 97.83%and a recall of 100%. 展开更多
关键词 Computer vision manual operation operation monitoring material counting
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On the Counting Functions of Meromorphic Solutions of Systems of Higher-order Algebraic Differential Equations
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作者 CHEN Miao-ling GAO Ling-yun 《Chinese Quarterly Journal of Mathematics》 CSCD 2011年第1期7-10,共4页
Using Nevanlinna theory and value distribution of meromorphic functions and the other techniques,we investigate the counting functions of meromorphic solutions of systems of higher-order algebraic differential equatio... Using Nevanlinna theory and value distribution of meromorphic functions and the other techniques,we investigate the counting functions of meromorphic solutions of systems of higher-order algebraic differential equations and obtain some results. 展开更多
关键词 meromorphic solution algebraic differential equations counting function
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Design of Fitness Movement Detection and Counting System Based on MediaPipe
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作者 Yinan Chen Xia Liu 《国际计算机前沿大会会议论文集》 EI 2023年第2期77-92,共16页
With the rapid advancements in artificial intelligence and computer vision technology,thefield of visual-based human pose detection has emerged as a highly sought-after research area in recent years.The identification of... With the rapid advancements in artificial intelligence and computer vision technology,thefield of visual-based human pose detection has emerged as a highly sought-after research area in recent years.The identification of human poses has practical applications in diverse domains,ranging from motion-sensing games for human-computer interaction to activity prediction and medical rehabil-itation.The present study is focused on the utilization of human pose detection forfitness movement counting.The ultimate aim of the system design is to accurately detect the skeletal key points of each body part in the image and subsequently con-nect them to form a human pose skeleton,which serves as a vital representation of the characteristics of human motion,particularly in the context of video data,where multiple human poses can be linked to form a certain movement trajectory.By judging the trajectory and angle changes,the system can determine whether people’sfitness movements are correct and help them improve theirfitness effec-tiveness.Hence,an increasing number of researchers are investing time and effort in thisfield.One common approach for human pose detection is OpenPose,but this model has a large and complex structure and low detection accuracy.Therefore,thisfitness movement detection and counting system uses a lightweight MediaPipe model and improves it to enhance the algorithm’s accuracy and recognition speed.The specific work in this paper includes three main points:(1)a suitable network structure to detect human skeletal points;(2)the appropriate skeletal structure forfitness movements through experiments to obtain accurate results;and(3)a Qt interface for human-computer interaction. 展开更多
关键词 Human Pose Detection Fitness Movement counting MediaPipe Skeletal Nodes
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Drone-based superconducting nanowire single-photon detection system with a detection efficiency of more than 90% 被引量:2
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作者 Ruoyan Ma Zhimin Guo +11 位作者 Dai Chen Xiaojun Dai You Xiao Chengjun Zhang Jiamin Xiong Jia Huang Xingyu Zhang Xiaoyu Liu Liangliang Rong Hao Li Xiaofu Zhang Lixing You 《Advanced Photonics Nexus》 2025年第2期25-30,共6页
Conventional superconducting nanowire single-photon detectors(SNSPDs)have been typically limited in their applications due to their size,weight,and power consumption,which confine their use to laboratory settings.Howe... Conventional superconducting nanowire single-photon detectors(SNSPDs)have been typically limited in their applications due to their size,weight,and power consumption,which confine their use to laboratory settings.However,with the rapid development of remote imaging,sensing technologies,and long-range quantum communication with fewer topographical constraints,the demand for high-efficiency single-photon detectors integrated with avionic platforms is rapidly growing.We herein designed and manufactured the first drone-based SNSPD system with a system detection efficiency(SDE)as high as 91.8%.This drone-based system incorporates high-performance NbTiN SNSPDs,a self-developed miniature liquid helium dewar,and custom-built integrated electrical setups,making it capable of being launched in complex topographical conditions.Such a drone-based SNSPD system may open the use of SNSPDs for applications that demand high SDE in complex environments. 展开更多
关键词 superconducting nanowire single-photon detector drone-based single-photon detection system high system detection efficiency dark count rate
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Plasmid-mediated transfer of antibiotic resistance genes and biofilm formation in a simulated drinking water distribution system under chlorine pressure 被引量:1
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作者 Yexing Wang Yingyu Zhang +2 位作者 Xiuneng Zhu Yulin Tang Yongji Zhang 《Journal of Environmental Sciences》 2025年第6期376-388,共13页
The effects of disinfectants and plasmid-based antibiotic resistance genes(ARGs)on the growth of microorganisms and the plasmid-mediated transfer of ARGs in the water and biofilm of the drinkingwater distribution syst... The effects of disinfectants and plasmid-based antibiotic resistance genes(ARGs)on the growth of microorganisms and the plasmid-mediated transfer of ARGs in the water and biofilm of the drinkingwater distribution system under simulated conditionswere explored.The heterotrophic plate count of the water in reactors with 0.1 mg/L NaClO and NH_(2)Cl was higher than in the control groups.Therewas no similar phenomenon in biofilm.In thewater of reactors containing NaClO,the aphA and bla geneswere lower than in the antibiotic resistant bacteria group,while both genes were higher in the water of reactors with NH_(2)Cl than in the control group.Chloramine may promote the transfer of ARGs in the water phase.Both genes in the biofilm of the reactors containing chlorine were lower than the control group.Correlation analysis between ARGs and water quality parameters revealed that the copy numbers of the aphA gene were significantly positively correlated with the copy numbers of the bla gene in water and significantly negatively correlated in biofilm(p<0.05).The results of the sequencing assay showed that bacteria in the biofilm,in the presence of disinfectant,were primarily Gram-negative.1.0 mg/L chlorine decreased the diversity of the community in the biofilm.The relative abundance of some bacteria that may undergo transfer increased in the biofilm of the reactor containing 0.1 mg/L chlorine. 展开更多
关键词 DISINFECTANT Antibiotic resistance genes(ARGs) Heterotrophic plate count(HPC) Drinking water distribution system Biofilm
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eDNA enhances detection efficiency but reveals lower waterbird diversity:A comparison with point counting method
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作者 Hongming Shuai Xiaoru Liu +3 位作者 Yigui Zhang Yuqi Sun Hao Li Zhongqiu Li 《Avian Research》 2025年第2期221-229,共9页
Environmental DNA(e DNA)methods have emerged as a promising tool for studying a broad spectrum of biological taxa.However,metabarcoding studies of avian biodiversity using e DNA have received little attention.In this ... Environmental DNA(e DNA)methods have emerged as a promising tool for studying a broad spectrum of biological taxa.However,metabarcoding studies of avian biodiversity using e DNA have received little attention.In this study,we compared waterbird biodiversity derived from e DNA metabarcoding with that obtained from traditional point counting surveys at 23 sites in Tai Lake of eastern China and evaluated the accuracy of e DNA metabarcoding for waterbird community studies.The point counting method recorded a higher total number of waterbird species(22)compared to the e DNA technique(16).While e DNA achieved a 74.5%detection rate for waterbird species and was able to identify a significantly greater number of species(12.48±1.97)at each sampling site than point counting method(6.13±2.69),particularly highlighting several rare and elusive species,it failed to detect some species commonly observed by the point counting method.The alpha diversity analysis revealed no significant differences in waterbird diversity between the e DNA method and the point counting method,except that the e DNA method exhibited lower Pielou evenness.Waterbird e DNA sequencing abundance correlated significantly with species occurrence,whereas Spearman's analysis indicated no significant difference between e DNA sequence abundance and species abundance from the point counting method.e DNA method detected no significant difference in waterbird composition between sampling sites,while the point counting method revealed significant differences.Consequently,e DNA is an effective complementary tool for assessing the diversity of wintering waterbirds in lakes,though it is unable to capture the full diversity of waterbird communities.It is crucial to develop sampling strategies that comprehensively monitor species composition and integrate e DNA with traditional survey methods for accurate evaluation of community structure. 展开更多
关键词 Environmental DNA Metabarcoding Point counting Species abundance Tai lake Waterbird diversity
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Delayed covering causes the accumulation of motile sperm, leading to overestimation of sperm concentration and motility with a Makler counting chamber
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作者 Lin Yu Qing-Yuan Cheng +4 位作者 Ye-Lin Jia Yan Zheng Ting-Ting Yang Ying-Bi Wu Fu-Ping Li 《Asian Journal of Andrology》 2025年第1期59-64,共6页
According to the World Health Organization(WHO)manual,sperm concentration should be measured using an improved Neubauer hemocytometer,while sperm motility should be measured by manual assessment.However,in China,thous... According to the World Health Organization(WHO)manual,sperm concentration should be measured using an improved Neubauer hemocytometer,while sperm motility should be measured by manual assessment.However,in China,thousands of laboratories do not use the improved Neubauer hemocytometer or method;instead,the Makler counting chamber is one of the most widely used chambers.To study sources of error that could impact the measurement of the apparent concentration and motility of sperm using the Makler counting chamber and to verify its accuracy for clinical application,67 semen samples from patients attending the Department of Andrology,West China Second University Hospital,Sichuan University(Chengdu,China)between 13 September 2023 and 27 September 2023,were included.Compared with applying the cover glass immediately,delaying the application of the cover glass for 5 s,10 s,and 30 s resulted in average increases in the sperm concentration of 30.3%,74.1%,and 107.5%,respectively(all P<0.0001)and in the progressive motility(PR)of 17.7%,30.8%,and 39.6%,respectively(all P<0.0001).However,when the semen specimens were fixed with formaldehyde,a delay in the application of the cover glass for 5 s,10 s,and 30 s resulted in an average increase in the sperm concentration of 6.7%,10.8%,and 14.6%,respectively,compared with immediate application of the cover glass.The accumulation of motile sperm due to delays in the application of the cover glass is a significant source of error with the Makler counting chamber and should be avoided. 展开更多
关键词 concentration improved Neubauer hemocytometer Makler counting chamber MOTILITY SPERM
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Commentary on“Delayed covering causes the accumulation of motile sperm,leading to overestimation of sperm concentration and motility with a Makler counting chamber”
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作者 Elisabetta Tosti 《Asian Journal of Andrology》 2025年第1期135-136,共2页
The analysis of the ejaculate,better known as spermiogram,represents the first and main step to identify whether a series of sperm quality parameters are within the norm and therefore are consistent with normal sperm ... The analysis of the ejaculate,better known as spermiogram,represents the first and main step to identify whether a series of sperm quality parameters are within the norm and therefore are consistent with normal sperm fertilizing capacity.Among these,sperm concentration and motility are the first parameters to be evaluated through an estimation carried out by expert examiners. 展开更多
关键词 identify whether series sperm quality parameters within delayed covering accumulation MOTILITY OVERESTIMATION makler counting chamber motile sperm sperm concentration
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Accurate counting of RNA in tissues with highly denoising amplified imaging
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作者 Xiaowen Cao Yuheng Zhu +2 位作者 Siyue Fan Feng Chen Yongxi Zhao 《Chinese Chemical Letters》 2025年第12期463-466,共4页
Characterization of the distribution and accurate counting of RNA molecules in the context of tissues is necessary to understand their complexity and heterogeneity.Single-molecule fluorescence in situ hybridization re... Characterization of the distribution and accurate counting of RNA molecules in the context of tissues is necessary to understand their complexity and heterogeneity.Single-molecule fluorescence in situ hybridization reveals the abundance and distribution of RNA and resolves different cell types in complex tissues.Especially,off-target binding and nonspecific adsorption of probes are prone to producing nonspecific amplification.Herein,we present highly de-noising amplified imaging,which leverages a sitespecific cleavage-amplifying design to achieve accurate counting of RNA in tissues.Our method avoids adding probe as primer,decreases nonspecific spots of single cells from 7 to nearly zero,and enables RNA imaging in uncleared tissue sections with nearly zero noise.We demonstrate the efficacy of this method on various thickness of mouse tissue sections.We envision this approach will serve as a tool to revealing the information content from patient samples for biomedical purpose. 展开更多
关键词 Nucleic acid amplification RNA endonuclease RNA counting Single-cell imaging Tissue sections
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A Method for Small Target Detection and Counting of the End of Drill Pipes Based on the Improved YOLO11n
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作者 Miao Li Xiaojun Li Mingyang Zhao 《Computers, Materials & Continua》 2025年第10期1917-1936,共20页
Aiming at problems such as large errors and low efficiency in manual counting of drill pipes during drilling depth measurement,an intelligent detection and counting method for the small targets at the end of drill pip... Aiming at problems such as large errors and low efficiency in manual counting of drill pipes during drilling depth measurement,an intelligent detection and counting method for the small targets at the end of drill pipes based on the improved YOLO11n is proposed.This method realizes the high-precision detection of targets at drill pipe ends in the image by optimizing the target detection model,and combines a post-processing correction mechanism to improve the drill pipe counting accuracy.In order to alleviate the low-precision problem of YOLO11n algorithm for small target recognition in the complex underground background,the YOLO11n algorithm is improved.First,the key module C3k2 in the backbone network was improved,and Poly Kernel Inception(PKI)Block was introduced to replace Bottleneck in it to fully integrate the target context information and the model’s capability of feature extraction;Second,within the model’s neck network,a new feature fusion pyramid ISOP(Improved Small Object Pyramid)is proposed,SPDConv is introduced to strengthen the P2 feature,and CSP and OmniKernel are combined to integrate multi-scale features;Finally,the default loss function is substituted with Powerful-IoU(PIoU)to solve the anchor box expansion problem.On the self-built dataset,experimental verification was conducted.The findings showed that the Recall rose by 6.4%,mAP@0.5 increased by 4.5%,and mAP@0.5:0.95 improved by 6%compared with the baseline model,effectively solving the issues of false detection and missed detection problems in small target detection task.Meanwhile,we conducted counting tests on drilling videos from 5 different scenarios,achieving an average accuracy of 97.3%,which meets the accuracy needs for drill pipe recognition and counting in coal mine drilling sites.The research findings offer theoretical basis and technical backing for promoting the intelligent development of coal mine gas extraction drilling sites. 展开更多
关键词 YOLO11n drill pipe counting small target PKI Block PIoU loss function
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