The use of artificial sweeteners is becoming increasingly common.High-fructose corn syrup(HFCS)and aspartame are the two most commonly used sweeteners.It is reported that their consumption was associated with the impa...The use of artificial sweeteners is becoming increasingly common.High-fructose corn syrup(HFCS)and aspartame are the two most commonly used sweeteners.It is reported that their consumption was associated with the impairment of cognitive function.However,the specific mechanism whereby HFCS and aspartame diets influence cognitive function is still unclear.Here,we administered 20%HFCS and 50 mg/kg aspartame in drinking water to establish a dietary intervention,and assessed mouse cognitive function using the novel object recognition(NOR)experiment.Results showed that the cognitive function of the mice declined in the NOR experiment.In order to clarify the molecular mechanisms,the Oxford Nanopore Technologies(ONT)platform was used to perform full-length RNA sequencing.As a result,662 long non-coding RNAs(lncRNAs)and 6895 alternative splicing(AS)events were identified,and the enrichment results of differentially expressed lncRNA target genes and AS genes suggested that the pathways related to cellular autophagy were enriched.The western blotting showed the PI3K/AKT/mTOR signaling pathway was activated,and the results of immunofluorescence also suggested that the HFCS and aspartame intake induced the accumulation of amyloid-βin the hippocampus.All in all,our study,starting from the dimensions of lncRNAs and AS,has deeply analyzed the potential mechanisms underlying the cognitive decline in mice caused by the intake of aspartame and HFCS,and put forward possible and novel insights.展开更多
High fructose corn syrup has been industrially produced by converting glucose to fructose by glucose isomerases,tetrameric metalloenzymes widely used in industrial biocatalysis.Advances in enzyme engineering and comme...High fructose corn syrup has been industrially produced by converting glucose to fructose by glucose isomerases,tetrameric metalloenzymes widely used in industrial biocatalysis.Advances in enzyme engineering and commercial production of glucose isomerase have paved the way to explore more efficient variants of these enzymes.The 5-hydroxymethylfurfural can be produced from high fructose corn syrup catalytic dehydration,and it can be further converted into various furanic compounds chemically or biologically for various industrial applications as a promising platform chemical.Although the chemical conversion of 5-hydroxymethylfurfural into furanic compounds has been extensively investigated in recent years,bioconversion has shown promise for its mild conditions due to the harsh chemical reaction conditions.This review discusses pro-tein engineering potential for improving glucose isomerase production and recent advancements in bioconversion of 5-hydroxymethylfurfural into value-added furanic derivatives.It suggests bi-ological strategies for the industrial transformation of 5-hydroxymethylfurfural.展开更多
文摘The use of artificial sweeteners is becoming increasingly common.High-fructose corn syrup(HFCS)and aspartame are the two most commonly used sweeteners.It is reported that their consumption was associated with the impairment of cognitive function.However,the specific mechanism whereby HFCS and aspartame diets influence cognitive function is still unclear.Here,we administered 20%HFCS and 50 mg/kg aspartame in drinking water to establish a dietary intervention,and assessed mouse cognitive function using the novel object recognition(NOR)experiment.Results showed that the cognitive function of the mice declined in the NOR experiment.In order to clarify the molecular mechanisms,the Oxford Nanopore Technologies(ONT)platform was used to perform full-length RNA sequencing.As a result,662 long non-coding RNAs(lncRNAs)and 6895 alternative splicing(AS)events were identified,and the enrichment results of differentially expressed lncRNA target genes and AS genes suggested that the pathways related to cellular autophagy were enriched.The western blotting showed the PI3K/AKT/mTOR signaling pathway was activated,and the results of immunofluorescence also suggested that the HFCS and aspartame intake induced the accumulation of amyloid-βin the hippocampus.All in all,our study,starting from the dimensions of lncRNAs and AS,has deeply analyzed the potential mechanisms underlying the cognitive decline in mice caused by the intake of aspartame and HFCS,and put forward possible and novel insights.
基金supported by the Natural Sciences and Engineering Research Council of Canada(Grant number RGPIN-2017-05366)to WQ.
文摘High fructose corn syrup has been industrially produced by converting glucose to fructose by glucose isomerases,tetrameric metalloenzymes widely used in industrial biocatalysis.Advances in enzyme engineering and commercial production of glucose isomerase have paved the way to explore more efficient variants of these enzymes.The 5-hydroxymethylfurfural can be produced from high fructose corn syrup catalytic dehydration,and it can be further converted into various furanic compounds chemically or biologically for various industrial applications as a promising platform chemical.Although the chemical conversion of 5-hydroxymethylfurfural into furanic compounds has been extensively investigated in recent years,bioconversion has shown promise for its mild conditions due to the harsh chemical reaction conditions.This review discusses pro-tein engineering potential for improving glucose isomerase production and recent advancements in bioconversion of 5-hydroxymethylfurfural into value-added furanic derivatives.It suggests bi-ological strategies for the industrial transformation of 5-hydroxymethylfurfural.
