To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuc...To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuclear transfer （SCNT） by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5’-triphosphate （ATP） content and reactive oxygen species （ROS） level in blastocysts were also analyzed.Firstly,for each type of blastocyst （IVF,ICSI or SCNT）,significant differences were observed between the survival rates of the controlled freezing （（81.56±2.33）,（68.18±4.72） or （47.89±5.83）%） and OPS vitrification groups （（92.24±4.54）,（82.40±3.76） or （78.71±5.91）%;P〈0.05）.Secondly,for each type of blastocyst （IVF,ICSI or SCNT）,ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group （0.43±0.06）,（0.35±0.05） or （0.21±0.02） pmol） was significantly lower than that found in the OPS vitrification group （0.62±0.04）,（0.46±0.03） or （0.30±0.01） pmol;P〈0.05）.Thirdly,ROS level in fresh IVF （（47.33±3.56） c.p.s （counted photons per second）,ICSI （（36.51±2.58） c.p.s） or SCNT blastocysts （（26.44±1.49） c.p.s） was significantly lower than that found in the OPS vitrification group （（72.14±4.31）,（58.89±3.89） or （40.11±5.73） c.p.s;P〈0.05）,but higher than that of the controlled freezing group （34.41±3.32）,（23.13±1.26） or （15.46±2.45） c.p.s;P〈0.05）.The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.展开更多
Controlled shear stress （CSS） test was used to study the effect of solid contents on the corresponding rheological parameters for sludge. Three types of sludge with or without conditioning, including activated slud...Controlled shear stress （CSS） test was used to study the effect of solid contents on the corresponding rheological parameters for sludge. Three types of sludge with or without conditioning, including activated sludge （AS）, anaerobic digested sludge （ADS）, and water treatment residuals （WTRs）, were collected for the CSS test. Results showed that the yield stress and the cohesion energy of the sludge networks were improved with increased total suspending solid （TSS） contents in most cases. For the conditioned AS/ADS and the raw WTRs, exponential law was observed in the relationships between cohesion energy of material networks or yield stress and the TSS contents, whereas for the conditioned WTRs, only exponential law dependence was found between the parameters of shear modulus or critical strain and the TSS contents.展开更多
During the 1970’s and 1980’s studies on the rela-tion between LAI and yield had been carried out inGAAS.The main results were as follows:(1)Usingthe accumulated LAI value at three stages,i.e.panicle initiation(PI),h...During the 1970’s and 1980’s studies on the rela-tion between LAI and yield had been carried out inGAAS.The main results were as follows:(1)Usingthe accumulated LAI value at three stages,i.e.panicle initiation(PI),heading(H)and 20 d afterheading(H),to analyze the relation between LAIand yield was more accurrate than only using theLAI value at H stage(see table);(2)Reaching cer-展开更多
基金supported by the Fund of China Agriculture Research System(CARS-37)the Genetically Modified Organisms Breeding Major Projects of China(2009ZX08011-031B)+1 种基金the Basic Research Fund of IAS,CAAS(2010jc-3-1)the National Natural Science Foundation of China(31001011)
文摘To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuclear transfer （SCNT） by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5’-triphosphate （ATP） content and reactive oxygen species （ROS） level in blastocysts were also analyzed.Firstly,for each type of blastocyst （IVF,ICSI or SCNT）,significant differences were observed between the survival rates of the controlled freezing （（81.56±2.33）,（68.18±4.72） or （47.89±5.83）%） and OPS vitrification groups （（92.24±4.54）,（82.40±3.76） or （78.71±5.91）%;P〈0.05）.Secondly,for each type of blastocyst （IVF,ICSI or SCNT）,ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group （0.43±0.06）,（0.35±0.05） or （0.21±0.02） pmol） was significantly lower than that found in the OPS vitrification group （0.62±0.04）,（0.46±0.03） or （0.30±0.01） pmol;P〈0.05）.Thirdly,ROS level in fresh IVF （（47.33±3.56） c.p.s （counted photons per second）,ICSI （（36.51±2.58） c.p.s） or SCNT blastocysts （（26.44±1.49） c.p.s） was significantly lower than that found in the OPS vitrification group （（72.14±4.31）,（58.89±3.89） or （40.11±5.73） c.p.s;P〈0.05）,but higher than that of the controlled freezing group （34.41±3.32）,（23.13±1.26） or （15.46±2.45） c.p.s;P〈0.05）.The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.
基金supported by the National Natural Science Foundation of China (No.51078035, 20977008)the Fundamental Research Funds for the Central Universities (No.JC2011-1, TD2010-5)+2 种基金the Ph.D.Programs Foundation of Ministry of Education of China (No.20100014110004)the High-Tech Research and Development Program (863)of China (No.2007AA06Z301)the Major Projects onthe Control and Rectification of Water Body Pollution (No.2008ZX07422-002-004, 2008ZX07314-006)
文摘Controlled shear stress （CSS） test was used to study the effect of solid contents on the corresponding rheological parameters for sludge. Three types of sludge with or without conditioning, including activated sludge （AS）, anaerobic digested sludge （ADS）, and water treatment residuals （WTRs）, were collected for the CSS test. Results showed that the yield stress and the cohesion energy of the sludge networks were improved with increased total suspending solid （TSS） contents in most cases. For the conditioned AS/ADS and the raw WTRs, exponential law was observed in the relationships between cohesion energy of material networks or yield stress and the TSS contents, whereas for the conditioned WTRs, only exponential law dependence was found between the parameters of shear modulus or critical strain and the TSS contents.
文摘During the 1970’s and 1980’s studies on the rela-tion between LAI and yield had been carried out inGAAS.The main results were as follows:(1)Usingthe accumulated LAI value at three stages,i.e.panicle initiation(PI),heading(H)and 20 d afterheading(H),to analyze the relation between LAIand yield was more accurrate than only using theLAI value at H stage(see table);(2)Reaching cer-
