Chemical investigation of the culture extract of an endophytic Penicillium citrinum from Dendrobium officinale,afforded nine citrinin derivatives(1–9)and one peptide-polyketide hybrid GKK1032B(10).The structures of t...Chemical investigation of the culture extract of an endophytic Penicillium citrinum from Dendrobium officinale,afforded nine citrinin derivatives(1–9)and one peptide-polyketide hybrid GKK1032B(10).The structures of these compounds were determined by spectroscopic methods.The absolute configurations of 1 and 2 were determined for the first time by calculation of electronic circular dichroism(ECD)data.Among them,GKK1032B(10)showed significant cytotoxicity against human osteosarcoma cell line MG63 with an IC50 value of 3.49μmol·L–1,and a primary mechanistic study revealed that it induced the apoptosis of MG63 cells via caspase pathway activation.展开更多
Perpyrrospirone A(1) characterized an unprecedented 6/5/6/8/5/13/6 oxahexacyclic scaffold with a unique peroxide-bridged 8,9-dioxa-2-azaspiro[4.7]dodecane core from marine-derived Penicillium citrinum. Compounds 2 and...Perpyrrospirone A(1) characterized an unprecedented 6/5/6/8/5/13/6 oxahexacyclic scaffold with a unique peroxide-bridged 8,9-dioxa-2-azaspiro[4.7]dodecane core from marine-derived Penicillium citrinum. Compounds 2 and 3 possessed rare oxatetracyclic(6/5/6/5) skeleton fused with a 13-menberedring macrocyclic moiety. Their structure and absolute configurations were determined by comprehensive spectroscopic analyses, ECD data coupled with TD-DFT calculations and X-ray diffraction experiments. In addition, 7 showed cytotoxicity and induced apoptosis of Hela cells in a dose-dependent manner after a48 h treatment.展开更多
Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived end...Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum HDN11-186.Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass spectra.The absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)spectrum.UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured fungi.Compound 1 showed obvious antibacterial activities against Pro-teus sp.MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).展开更多
This report provides for the first time rapid novel environment friendly cell surface based synthesis of stable gold nanoprisms at room temperature using Penicillium citrinum MTCC9999 biomass. The UV-Visible spectral ...This report provides for the first time rapid novel environment friendly cell surface based synthesis of stable gold nanoprisms at room temperature using Penicillium citrinum MTCC9999 biomass. The UV-Visible spectral scan of dispersed gold nanoparticles (GNPs) solution showed absorption maxima at 540 nm due to surface plasma resonance (SPR) of gold nanoparticles. Typical Transmission Electron Microscopic (TEM) images showed that most of them were prism (55%) shaped with a diameter ranging from 20 - 40 nm. These results obtained from TEM correlated well with the data obtained from Dynamic Light Scattering (DLS) experiment. Average zeta potential of GNPs was -20 mV suggesting some biomolecules capped the nanoparticles imparting a net negative charge over it. FTIR analysis also showed that protein molecules were involved in stabilization.展开更多
[Objectives]The research was to explore the antifungal effects of citronellal,wintergreen oil and their composite essence on Penicillium citrinum.[Methods]The inhibition zones of citronellal,wintergreen oil and their ...[Objectives]The research was to explore the antifungal effects of citronellal,wintergreen oil and their composite essence on Penicillium citrinum.[Methods]The inhibition zones of citronellal,wintergreen oil and their composite essence against P.citrinum were determined by disk diffusion method,and the growth of inhibition zone plate was observed.The fumigation fungicidal rate of fungi-bearing rice paper within 7 d was determined by plate counting method.[Results]Citronellal had strong antifungal effect,and wintergreen oil could slow down the growth rate of P.citrinum.The combination of citronellal and wintergreen oil had a synergistic effect.As fumigants,both citronellal and composite essence had fungicidal rates up to 100%within 7 d.Combined with anaerobic treatments,all the three essential oils could improve the fungicidal efficiency.The adsorbent in deoxidizing agent would adsorb the essential oils,so that their fumigation had a certain slow release effect.[Conclusions]This study can provide a more friendly and efficient method for the prevention and control of cultural relics mold.展开更多
Citrinin is a common mycotoxin produced by different fungi.Although considerable endeavors have been made to increase citrinin output to meet the requirements of research and industrial needs,few methods are effective...Citrinin is a common mycotoxin produced by different fungi.Although considerable endeavors have been made to increase citrinin output to meet the requirements of research and industrial needs,few methods are effective,owing to potential safety concerns and technical limitations.In this study,a blue fungus that produces a yellow pigment was isolated from Ziziphus jujuba.The strain was then identified as Penicillium citrinum and named HR-087.Preparative liquid chromatography was used to extract the yellow pigment.Both LC-MS and NMR assays showed that it was citrinin.To enhance the citrinin pro-duction of this strain,the culture medium was optimized through orthogonal experiments,as well as batch and fed-batch process.The final titer and productivity of citrinin reached 9.62 g/L and 0.1 g/(L×h),respectively.The high titer achieved indicates the possibility of large-scale production of high purity citrinin for low-cost supply for both academic and diagnostic analysis standard.展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.21462015,31760093)the National Natural Science Foundation of Hainan Province(No.218MS045)the Program for Innovative Research Team in University(No.IRT-16R19)~~
基金the National Natural Science Foundation of China(Nos.82073728 and 31501104)the Natural Science Foundation of Shandong Province(Nos.ZR2019QH006 and JQ201721)+3 种基金the Project of Shandong Province Higher Educational Science and Technology Program(No.J17KA228)the Innovation Team Project of Jinan Science&Technology Bureau(No.2018GXRC003)the Young Taishan Scholars Program(No.tsqn20161037)Shandong Talents Team Cultivation Plan of University Preponderant Discipline(No.10027).
文摘Chemical investigation of the culture extract of an endophytic Penicillium citrinum from Dendrobium officinale,afforded nine citrinin derivatives(1–9)and one peptide-polyketide hybrid GKK1032B(10).The structures of these compounds were determined by spectroscopic methods.The absolute configurations of 1 and 2 were determined for the first time by calculation of electronic circular dichroism(ECD)data.Among them,GKK1032B(10)showed significant cytotoxicity against human osteosarcoma cell line MG63 with an IC50 value of 3.49μmol·L–1,and a primary mechanistic study revealed that it induced the apoptosis of MG63 cells via caspase pathway activation.
基金funded by the National Natural Science Foundation of China (Nos. 41876189, 81703388)the National Key R&D Program of China (Nos. 2018YFC1706206, 2019YFC0312504)supported by the Open Research Fund Program of State Key Laboratory of Bioreactor Engineering and Institute of Regulatory Science。
文摘Perpyrrospirone A(1) characterized an unprecedented 6/5/6/8/5/13/6 oxahexacyclic scaffold with a unique peroxide-bridged 8,9-dioxa-2-azaspiro[4.7]dodecane core from marine-derived Penicillium citrinum. Compounds 2 and 3 possessed rare oxatetracyclic(6/5/6/5) skeleton fused with a 13-menberedring macrocyclic moiety. Their structure and absolute configurations were determined by comprehensive spectroscopic analyses, ECD data coupled with TD-DFT calculations and X-ray diffraction experiments. In addition, 7 showed cytotoxicity and induced apoptosis of Hela cells in a dose-dependent manner after a48 h treatment.
基金supported by the National Natural Science Foundation of China(No.41806167)the High-Level Talents Research Fund of Qingdao Agricultural University(No.665/1120034)+4 种基金the NSFC-Shandong Joint Fund(No.U1906212)the Major Project of the 14th Five-Year Plan(No.2022QNLM030003-1)the Natural Science Foundation of Shandong Province(No.ZR2021ZD28)the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(No.2021CXLH0012)the Youth Innovation Plan of Shandong Province(No.2019KJM004).
文摘Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum HDN11-186.Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass spectra.The absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)spectrum.UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured fungi.Compound 1 showed obvious antibacterial activities against Pro-teus sp.MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).
文摘This report provides for the first time rapid novel environment friendly cell surface based synthesis of stable gold nanoprisms at room temperature using Penicillium citrinum MTCC9999 biomass. The UV-Visible spectral scan of dispersed gold nanoparticles (GNPs) solution showed absorption maxima at 540 nm due to surface plasma resonance (SPR) of gold nanoparticles. Typical Transmission Electron Microscopic (TEM) images showed that most of them were prism (55%) shaped with a diameter ranging from 20 - 40 nm. These results obtained from TEM correlated well with the data obtained from Dynamic Light Scattering (DLS) experiment. Average zeta potential of GNPs was -20 mV suggesting some biomolecules capped the nanoparticles imparting a net negative charge over it. FTIR analysis also showed that protein molecules were involved in stabilization.
基金National Key Research and Development Program of China(2020YFC1522500)Special Project of Technical Innovation and Application Development of Chongqing City(cstc2020jscx-msxmX0097)。
文摘[Objectives]The research was to explore the antifungal effects of citronellal,wintergreen oil and their composite essence on Penicillium citrinum.[Methods]The inhibition zones of citronellal,wintergreen oil and their composite essence against P.citrinum were determined by disk diffusion method,and the growth of inhibition zone plate was observed.The fumigation fungicidal rate of fungi-bearing rice paper within 7 d was determined by plate counting method.[Results]Citronellal had strong antifungal effect,and wintergreen oil could slow down the growth rate of P.citrinum.The combination of citronellal and wintergreen oil had a synergistic effect.As fumigants,both citronellal and composite essence had fungicidal rates up to 100%within 7 d.Combined with anaerobic treatments,all the three essential oils could improve the fungicidal efficiency.The adsorbent in deoxidizing agent would adsorb the essential oils,so that their fumigation had a certain slow release effect.[Conclusions]This study can provide a more friendly and efficient method for the prevention and control of cultural relics mold.
基金This work was supported by the National Key Research and Development Program of China(2018YFC1604102)the National Science Fund for Excellent Young Scholars(21822806).
文摘Citrinin is a common mycotoxin produced by different fungi.Although considerable endeavors have been made to increase citrinin output to meet the requirements of research and industrial needs,few methods are effective,owing to potential safety concerns and technical limitations.In this study,a blue fungus that produces a yellow pigment was isolated from Ziziphus jujuba.The strain was then identified as Penicillium citrinum and named HR-087.Preparative liquid chromatography was used to extract the yellow pigment.Both LC-MS and NMR assays showed that it was citrinin.To enhance the citrinin pro-duction of this strain,the culture medium was optimized through orthogonal experiments,as well as batch and fed-batch process.The final titer and productivity of citrinin reached 9.62 g/L and 0.1 g/(L×h),respectively.The high titer achieved indicates the possibility of large-scale production of high purity citrinin for low-cost supply for both academic and diagnostic analysis standard.
