Drugs that lack the ability to cross the blood- brain barrier （BBB） need to be placed directly into the central nervous system. Our laboratory studies the involve- ment of the glutamatergic system in the aggressiven...Drugs that lack the ability to cross the blood- brain barrier （BBB） need to be placed directly into the central nervous system. Our laboratory studies the involve- ment of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebel- lomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solu- tion. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.展开更多
Introduction: Suprasellar cistern arachnoid cysts are rare non-neoplastic cystic lesions that represent nearly 1% of all intracranial arachnoid cysts. Symptomatology can vary;small cysts typically do not produce any s...Introduction: Suprasellar cistern arachnoid cysts are rare non-neoplastic cystic lesions that represent nearly 1% of all intracranial arachnoid cysts. Symptomatology can vary;small cysts typically do not produce any symptoms, whereas larger cysts may lead to headaches, blurred vision, and other related issues. However, the etiology, pathogenesis, and natural history of this condition remain unclear.Case presentation: This study includes four cases from our hospital from the year 2016 to 2021, first diagnosed with hydrocephalus received ventriculoperitoneal shunt treatments, the patients complicated with enlargement of the suprasellar cisterna arachnoid cyst occurred following ventriculoperitoneal shunt placement, leading to secondary obstructive hydrocephalus and received neuroendoscopic third ventriculostomy and cyst fenestration.Conclusion: Suprasellar cisterna arachnoid cysts may be related to intracranial pressure dynamics. Fluctuations in intracranial pressure can lead to variations in cyst size and may result in associated symptoms. Monitoring imaging and symptoms before, during, and after surgery, along with comprehensive follow-up, is important.展开更多
Since the first electron micrograph of“lace-like structures”over 75 years ago,the endoplasmic reticulum(ER)is now viewed as a highly dynamic,constantly remodeling,continuous network of tubules and cisternae that pla...Since the first electron micrograph of“lace-like structures”over 75 years ago,the endoplasmic reticulum(ER)is now viewed as a highly dynamic,constantly remodeling,continuous network of tubules and cisternae that plays an important role in a broad range of cellular activities from calcium regulation to protein synthesis and trafficking.In neurons,the ER extends from the soma through the axon to presynaptic terminals,and throughout the dendritic arbor into as many as half of all postsynaptic dendritic spines at any given time(Falahati et al.,2022).展开更多
INTRODUCTION The cisterna magna is a space lying between the cerebellum and medulla oblongata that is important in collecting cerebral spinal fluid （CSF） in some patients with contraindication or the impossibility o...INTRODUCTION The cisterna magna is a space lying between the cerebellum and medulla oblongata that is important in collecting cerebral spinal fluid （CSF） in some patients with contraindication or the impossibility of lumbar puncture and in intrathecal administration medication infusion.展开更多
Peripheral cisternae and double membranes(PCDMs)in erythroid cells are a landmark of typeⅡcongenital dyserythropoietic anemia(CDA).To gain further insights into the mechanism of dyserythropoiesis,erythroblasts and er...Peripheral cisternae and double membranes(PCDMs)in erythroid cells are a landmark of typeⅡcongenital dyserythropoietic anemia(CDA).To gain further insights into the mechanism of dyserythropoiesis,erythroblasts and erythrocytes in bone marrow were studied in 22 Chinese patients with CDAⅡby transmission electron microscopy.The study demonstrated an increase in all patients in erythroblasts with PCDMs with development from pro-erythroblast to red blood cells.PCDMs often connected with cisternae of endoplasmic reticulum(ER)and the perinuclear space,and were accompanied by karyopyknosis,karyolysis and disruption in polychromatic and orthochromatic erythroblasts.The results suggest that PCDMs are transformed from ER during erythropoiesis and participate in the dissolution and deletion of late erythroid cells in patients with CDAⅡ.展开更多
基金supported by Coordenacao de Aperfeicoamento de Pessoal de Nível Superior(CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)-Edital Doencas Neurodegenerativas+1 种基金Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul(FAPERGS)Financiadora de Estados e Projetos(FINEP)
文摘Drugs that lack the ability to cross the blood- brain barrier （BBB） need to be placed directly into the central nervous system. Our laboratory studies the involve- ment of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebel- lomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solu- tion. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.
文摘Introduction: Suprasellar cistern arachnoid cysts are rare non-neoplastic cystic lesions that represent nearly 1% of all intracranial arachnoid cysts. Symptomatology can vary;small cysts typically do not produce any symptoms, whereas larger cysts may lead to headaches, blurred vision, and other related issues. However, the etiology, pathogenesis, and natural history of this condition remain unclear.Case presentation: This study includes four cases from our hospital from the year 2016 to 2021, first diagnosed with hydrocephalus received ventriculoperitoneal shunt treatments, the patients complicated with enlargement of the suprasellar cisterna arachnoid cyst occurred following ventriculoperitoneal shunt placement, leading to secondary obstructive hydrocephalus and received neuroendoscopic third ventriculostomy and cyst fenestration.Conclusion: Suprasellar cisterna arachnoid cysts may be related to intracranial pressure dynamics. Fluctuations in intracranial pressure can lead to variations in cyst size and may result in associated symptoms. Monitoring imaging and symptoms before, during, and after surgery, along with comprehensive follow-up, is important.
基金supported by AHA Career Development Award 938683 (to PJD)NIH grant R01MH123700 (to MLD)
文摘Since the first electron micrograph of“lace-like structures”over 75 years ago,the endoplasmic reticulum(ER)is now viewed as a highly dynamic,constantly remodeling,continuous network of tubules and cisternae that plays an important role in a broad range of cellular activities from calcium regulation to protein synthesis and trafficking.In neurons,the ER extends from the soma through the axon to presynaptic terminals,and throughout the dendritic arbor into as many as half of all postsynaptic dendritic spines at any given time(Falahati et al.,2022).
文摘INTRODUCTION The cisterna magna is a space lying between the cerebellum and medulla oblongata that is important in collecting cerebral spinal fluid （CSF） in some patients with contraindication or the impossibility of lumbar puncture and in intrathecal administration medication infusion.
文摘Peripheral cisternae and double membranes(PCDMs)in erythroid cells are a landmark of typeⅡcongenital dyserythropoietic anemia(CDA).To gain further insights into the mechanism of dyserythropoiesis,erythroblasts and erythrocytes in bone marrow were studied in 22 Chinese patients with CDAⅡby transmission electron microscopy.The study demonstrated an increase in all patients in erythroblasts with PCDMs with development from pro-erythroblast to red blood cells.PCDMs often connected with cisternae of endoplasmic reticulum(ER)and the perinuclear space,and were accompanied by karyopyknosis,karyolysis and disruption in polychromatic and orthochromatic erythroblasts.The results suggest that PCDMs are transformed from ER during erythropoiesis and participate in the dissolution and deletion of late erythroid cells in patients with CDAⅡ.
