目的:探讨动态对比增强磁共振成像(dynamic contrast-enhanced magnetic resonance imaging,DCE-MRI)定量参数、血清环状RNA-PVT1(circular RNA-PVT1,circPVT1)、环状RNA-DUSP16(circular RNA-DUST16,circDUSP16)水平与胃癌患者病理特...目的:探讨动态对比增强磁共振成像(dynamic contrast-enhanced magnetic resonance imaging,DCE-MRI)定量参数、血清环状RNA-PVT1(circular RNA-PVT1,circPVT1)、环状RNA-DUSP16(circular RNA-DUST16,circDUSP16)水平与胃癌患者病理特征的相关性及临床意义。方法:回顾性选取2022年1月至2024年3月106例镇平县人民医院影像科胃癌患者为恶性组,选取同期同科室106例胃部良性肿瘤患者为良性组。比较组两DCE-MRI定量参数[胃癌容积转移常数(volume transfer constant,K^(trans))、血管外间隙容积比(extravascular gap volume ratio,Ve)、速率常数(rate constant,K_(ep))]、血清circPVT1、circDUSP16水平,和恶性组中DCE-MRI定量参数、血清circPVT1、circDUSP16不同水平患者的临床病理特征。比较不同预后患者临床病理特征、DCE-MRI定量参数、血清circPVT1、circDUSP16水平并分析胃癌患者发生不良预后的影响因素。结果:恶性组K^(trans)、V_(e)、血清circPVT1、circDUSP16水平高于良性组,K_(ep)低于良性组(P<0.05);在病理分期Ⅲ~Ⅳ期、低分化程度、有淋巴结转移例数占比方面相比,K^(trans)、V_(e)、血清circPVT1、circDUSP16高水平患者高于低水平患者,K_(ep)高水平患者低于低水平患者,预后不良患者高于预后良好患者(P<0.05);预后不良患者K^(trans)、V_(e)、血清circPVT1、circDUSP16水平高于预后良好患者,K_(ep)低于预后良好患者(P<0.05);病理分期Ⅲ~Ⅳ期、低分化程度、有淋巴结转移、K^(trans)≥0.19min^(-1)、Ve≥0.45、K_(ep)≥0.47min^(-1)、circPVT1≥4.45、circDUSP16≥1.72均为预后不良的危险因素(P<0.05)。结论:DCE-MRI定量参数、血清circPVT1、circDUSP16水平与胃癌患者临床病理特征、患者预后关系密切,可作为临床预测患者预后的潜在指标。展开更多
Epithelial-mesenchymal transition(EMT)is a vital pathological feature of silica-induced pulmonary fibrosis.However,whether circRNA is involved in the process remains unclear.The present study aimed to investigate the ...Epithelial-mesenchymal transition(EMT)is a vital pathological feature of silica-induced pulmonary fibrosis.However,whether circRNA is involved in the process remains unclear.The present study aimed to investigate the role of circPVT1 in the silica-induced EMT and the underlying mechanisms.We found that an elevated expression of circPVT1 promoted EMT and enhanced the migratory capacity of silica-treated epithelial cells.The isolation of cytoplasmic and nuclear separation assay showed that circPVT1 was predominantly expressed in the cytoplasm.RNA immunoprecipitation assay and RNA pull-down experiment indicated that cytoplasmic-localized circPVT1 was capable of binding to miR-497-5p.Furthermore,we found that miR-497-5p attenuated the silica-induced EMT process by targeting transcription factor 3(TCF3),an E-cadherin transcriptional repressor,in the silica-treated epithelial cells.Collectively,these results reveal a novel role of the circPVT1/miR-497-5p/TCF3 axis in the silica-induced EMT process in lung epithelial cells.Once validated,this finding may provide a potential theoretical basis for the development of interventions and treatments for pulmonary fibrosis.展开更多
Plasmacytoma variant translocation 1 (PVT1) is a long non-coding RNA (lncRNA) gene identified as a recurrent breakpoint of Burkitt’s lymphomas. Human PVT1 gene is located on region 8q24.21, a well-known cancer risk r...Plasmacytoma variant translocation 1 (PVT1) is a long non-coding RNA (lncRNA) gene identified as a recurrent breakpoint of Burkitt’s lymphomas. Human PVT1 gene is located on region 8q24.21, a well-known cancer risk region, and encodes at least 26 linear ncRNA isoforms and 26 circular RNA isoforms, as well as 6 microRNAs. Several PVT1 functioning models have been reported recently such as competing endogenous RNA (ceRNA) activity and regulating protein stability of oncogenes, especially MYC oncogene. The promoter of PVT1 gene is a boundary element of tumor-suppressor DNA. CircPVT1 derived from PVT1 gene is also a critical non-coding oncogenic RNA. Although substantial advancements have been made in understanding the roles of PVT1 in cancer recently, the detailed mechanisms underlying its functions remain unclear. Herein, we summarize the recent progressions on the mechanisms underlying PVT1 regulated gene expression at different levels. We also discuss the interaction between lncRNA and protein, RNA and DNA, as well as the potential cancer therapy strategy by targeting these networks.展开更多
文摘目的:探讨动态对比增强磁共振成像(dynamic contrast-enhanced magnetic resonance imaging,DCE-MRI)定量参数、血清环状RNA-PVT1(circular RNA-PVT1,circPVT1)、环状RNA-DUSP16(circular RNA-DUST16,circDUSP16)水平与胃癌患者病理特征的相关性及临床意义。方法:回顾性选取2022年1月至2024年3月106例镇平县人民医院影像科胃癌患者为恶性组,选取同期同科室106例胃部良性肿瘤患者为良性组。比较组两DCE-MRI定量参数[胃癌容积转移常数(volume transfer constant,K^(trans))、血管外间隙容积比(extravascular gap volume ratio,Ve)、速率常数(rate constant,K_(ep))]、血清circPVT1、circDUSP16水平,和恶性组中DCE-MRI定量参数、血清circPVT1、circDUSP16不同水平患者的临床病理特征。比较不同预后患者临床病理特征、DCE-MRI定量参数、血清circPVT1、circDUSP16水平并分析胃癌患者发生不良预后的影响因素。结果:恶性组K^(trans)、V_(e)、血清circPVT1、circDUSP16水平高于良性组,K_(ep)低于良性组(P<0.05);在病理分期Ⅲ~Ⅳ期、低分化程度、有淋巴结转移例数占比方面相比,K^(trans)、V_(e)、血清circPVT1、circDUSP16高水平患者高于低水平患者,K_(ep)高水平患者低于低水平患者,预后不良患者高于预后良好患者(P<0.05);预后不良患者K^(trans)、V_(e)、血清circPVT1、circDUSP16水平高于预后良好患者,K_(ep)低于预后良好患者(P<0.05);病理分期Ⅲ~Ⅳ期、低分化程度、有淋巴结转移、K^(trans)≥0.19min^(-1)、Ve≥0.45、K_(ep)≥0.47min^(-1)、circPVT1≥4.45、circDUSP16≥1.72均为预后不良的危险因素(P<0.05)。结论:DCE-MRI定量参数、血清circPVT1、circDUSP16水平与胃癌患者临床病理特征、患者预后关系密切,可作为临床预测患者预后的潜在指标。
基金funded by the National Natural Science Foundation of China(Grant No.82073518).
文摘Epithelial-mesenchymal transition(EMT)is a vital pathological feature of silica-induced pulmonary fibrosis.However,whether circRNA is involved in the process remains unclear.The present study aimed to investigate the role of circPVT1 in the silica-induced EMT and the underlying mechanisms.We found that an elevated expression of circPVT1 promoted EMT and enhanced the migratory capacity of silica-treated epithelial cells.The isolation of cytoplasmic and nuclear separation assay showed that circPVT1 was predominantly expressed in the cytoplasm.RNA immunoprecipitation assay and RNA pull-down experiment indicated that cytoplasmic-localized circPVT1 was capable of binding to miR-497-5p.Furthermore,we found that miR-497-5p attenuated the silica-induced EMT process by targeting transcription factor 3(TCF3),an E-cadherin transcriptional repressor,in the silica-treated epithelial cells.Collectively,these results reveal a novel role of the circPVT1/miR-497-5p/TCF3 axis in the silica-induced EMT process in lung epithelial cells.Once validated,this finding may provide a potential theoretical basis for the development of interventions and treatments for pulmonary fibrosis.
文摘Plasmacytoma variant translocation 1 (PVT1) is a long non-coding RNA (lncRNA) gene identified as a recurrent breakpoint of Burkitt’s lymphomas. Human PVT1 gene is located on region 8q24.21, a well-known cancer risk region, and encodes at least 26 linear ncRNA isoforms and 26 circular RNA isoforms, as well as 6 microRNAs. Several PVT1 functioning models have been reported recently such as competing endogenous RNA (ceRNA) activity and regulating protein stability of oncogenes, especially MYC oncogene. The promoter of PVT1 gene is a boundary element of tumor-suppressor DNA. CircPVT1 derived from PVT1 gene is also a critical non-coding oncogenic RNA. Although substantial advancements have been made in understanding the roles of PVT1 in cancer recently, the detailed mechanisms underlying its functions remain unclear. Herein, we summarize the recent progressions on the mechanisms underlying PVT1 regulated gene expression at different levels. We also discuss the interaction between lncRNA and protein, RNA and DNA, as well as the potential cancer therapy strategy by targeting these networks.
