Little information is available about the effects of exposure to pulsed microwaves on neuronal Ca^2＋ signaling under non-thermal conditions. In this study, rat pheochromocytoma （PC12） cells were exposed to pulsed m...Little information is available about the effects of exposure to pulsed microwaves on neuronal Ca^2＋ signaling under non-thermal conditions. In this study, rat pheochromocytoma （PC12） cells were exposed to pulsed microwaves for 6 min at a specific absorption rate （SAR） of 4 W/kg to assess possible real-time effects. During microwave exposure, free calcium dynamics in the cytosol, mitochondria, and nucleus of cells were monitored by time-lapse microfluorimetry using a genetically encoded calcium indicator （ratiometric-pericam, ratiometric-10ericam-mt,展开更多
Objective To investigate the relationship between serum calcium levels and pain in patientswith Parkinson's disease(PD).Methods A total of 111 patients with PD and 50 healthy volunteers were recruited from our hos...Objective To investigate the relationship between serum calcium levels and pain in patientswith Parkinson's disease(PD).Methods A total of 111 patients with PD and 50 healthy volunteers were recruited from our hospital between July 2019 and June 2020.Motor symptoms of PD patients were assessed using the Hoehn-Yahr(H&Y)stages and the Unified Parkinson's Disease Ratingg Scale-IIl(UPDRS II).Non-motor symptomswere evaluatedusing Mini-MentalState Examination(MMSE),17-item Hamilton Depression Rating Scale(HAMD-17),14-item Hamilton Anxiety Rating Scale(HAMA-14),questionnaire for rapid eye movement(REM)sleep behavior disorder(RBDQ-HK),King Parkinson's pain scale(KPPS),Pittsburgh Sleep Quality Index(PSQI),Parkinson's Disease Sleep Scale(PDSS),and Epworth Sleepiness Scale(ESS).The quality of life of PD patients was assessed using the 39-item Parkinson's Disease Questionnaire(PDQ-39).Results The levels of serum calcium in PD patients were significantly lower than those in the control group(t=3.733,P<0.001).Additionally,the levels of serum calcium in PD patients with pain were higher than those in PD patients without pain(t=-3.238,P<0.05).This suggests a significant positive correlation between serum calcium levels and pain in PD patients(r=0.320,P=0.001).When analyzing serum calcium levels for PD with pain using binary logistic regression,the area under the curve(AUC=0.662)and sensitivity(28.9%)were found to be low.Furthermore,a correlation analysis of KPPS scores in PD patients with pain revealed that KPPS scores were correlated with UPDRS II(r=0.383,P=0.009),HAMD-17(r=0.303,P=0.043),HAMA-14(r=0.303,P=0.043),PSQI(r=0.304,P=0.042),and PDSS(r=-0.417,P=0.004)scores.Conclusion The levels of serum calcium are decreased in patients with Parkinson's disease(PD),and there is a correlation between serum calcium levels and pain experienced by PD patients.However,it is important to note that pain in PD patients is influenced by various other factors.展开更多
Calcium homeostasis in synaptosomes is altered during aging. The intrasynaptomal free calcium concentration ([Ca2+]i) was determined in 3- and 24-month-old rats treated with or without Rbl and Rgl. As expected, the [C...Calcium homeostasis in synaptosomes is altered during aging. The intrasynaptomal free calcium concentration ([Ca2+]i) was determined in 3- and 24-month-old rats treated with or without Rbl and Rgl. As expected, the [Ca2-]i level increased with age. Treatment with Rbl and Rgl elicited an obvious decrease of [Ca2+]i content, especially in aged rates. In addition, Rbl and Rgl significantly stimulated the activity of Na+, K+-ATPase while Rbl inhibited the activity of Ca2+, Mg2--ATPase arid calmodulin. In view of the close relationship of [Ca2-]i level with aging, the changes of [Ca2+]i induced by Rbl and Rgl, as shown by our results, might provide an explanation of the mechanisms of their antiaging function.展开更多
The effects of Arecoline (Are) on calcium m obilization were investigated. In isolated single ventricular m yocyte of guinea pig,patch clamp whole cell recording techniques were used to record the current of L - typ...The effects of Arecoline (Are) on calcium m obilization were investigated. In isolated single ventricular m yocyte of guinea pig,patch clamp whole cell recording techniques were used to record the current of L - type calcium channel and cytosolic Ca2 + level ([Ca2 + ]i) labeled with fluo- rescence probe Fluo- 3/ AM was m easured under a laser scanning confocal microscope.Results re- vealed that Are(3- 10 0 μm ol/ L) could inhibit L- type calcium current in a concentration- depen- dent manner and the value of IC50 was33.73μm ol/ L (n=5 ) .In the absence of extracellular calci- um,the resting levels of[Ca2 + ]i was not affected by Are(n=6 ,P>0 .0 5 ) ,but pretreatment with Are(30 μmol/ L) could significantly inhibit the[Ca2 + ]i elevation induced by caffeine(10 m mol/ L,n=6 ,P<0 .0 1) .It was concluded that Are could inhibit not only calcium influx through L- type calcium channel but also calcium release from sarcoplasm ic reticulum.展开更多
基金supported by the National Natural Science Foundation of China[No.31400716 and No.61401497]
文摘Little information is available about the effects of exposure to pulsed microwaves on neuronal Ca^2＋ signaling under non-thermal conditions. In this study, rat pheochromocytoma （PC12） cells were exposed to pulsed microwaves for 6 min at a specific absorption rate （SAR） of 4 W/kg to assess possible real-time effects. During microwave exposure, free calcium dynamics in the cytosol, mitochondria, and nucleus of cells were monitored by time-lapse microfluorimetry using a genetically encoded calcium indicator （ratiometric-pericam, ratiometric-10ericam-mt,
文摘Objective To investigate the relationship between serum calcium levels and pain in patientswith Parkinson's disease(PD).Methods A total of 111 patients with PD and 50 healthy volunteers were recruited from our hospital between July 2019 and June 2020.Motor symptoms of PD patients were assessed using the Hoehn-Yahr(H&Y)stages and the Unified Parkinson's Disease Ratingg Scale-IIl(UPDRS II).Non-motor symptomswere evaluatedusing Mini-MentalState Examination(MMSE),17-item Hamilton Depression Rating Scale(HAMD-17),14-item Hamilton Anxiety Rating Scale(HAMA-14),questionnaire for rapid eye movement(REM)sleep behavior disorder(RBDQ-HK),King Parkinson's pain scale(KPPS),Pittsburgh Sleep Quality Index(PSQI),Parkinson's Disease Sleep Scale(PDSS),and Epworth Sleepiness Scale(ESS).The quality of life of PD patients was assessed using the 39-item Parkinson's Disease Questionnaire(PDQ-39).Results The levels of serum calcium in PD patients were significantly lower than those in the control group(t=3.733,P<0.001).Additionally,the levels of serum calcium in PD patients with pain were higher than those in PD patients without pain(t=-3.238,P<0.05).This suggests a significant positive correlation between serum calcium levels and pain in PD patients(r=0.320,P=0.001).When analyzing serum calcium levels for PD with pain using binary logistic regression,the area under the curve(AUC=0.662)and sensitivity(28.9%)were found to be low.Furthermore,a correlation analysis of KPPS scores in PD patients with pain revealed that KPPS scores were correlated with UPDRS II(r=0.383,P=0.009),HAMD-17(r=0.303,P=0.043),HAMA-14(r=0.303,P=0.043),PSQI(r=0.304,P=0.042),and PDSS(r=-0.417,P=0.004)scores.Conclusion The levels of serum calcium are decreased in patients with Parkinson's disease(PD),and there is a correlation between serum calcium levels and pain experienced by PD patients.However,it is important to note that pain in PD patients is influenced by various other factors.
文摘Calcium homeostasis in synaptosomes is altered during aging. The intrasynaptomal free calcium concentration ([Ca2+]i) was determined in 3- and 24-month-old rats treated with or without Rbl and Rgl. As expected, the [Ca2-]i level increased with age. Treatment with Rbl and Rgl elicited an obvious decrease of [Ca2+]i content, especially in aged rates. In addition, Rbl and Rgl significantly stimulated the activity of Na+, K+-ATPase while Rbl inhibited the activity of Ca2+, Mg2--ATPase arid calmodulin. In view of the close relationship of [Ca2-]i level with aging, the changes of [Ca2+]i induced by Rbl and Rgl, as shown by our results, might provide an explanation of the mechanisms of their antiaging function.
基金Thisprojectwassupported by a grant from Natural Sci-ences Foundation of China(No. 396 70 6 6 1)
文摘The effects of Arecoline (Are) on calcium m obilization were investigated. In isolated single ventricular m yocyte of guinea pig,patch clamp whole cell recording techniques were used to record the current of L - type calcium channel and cytosolic Ca2 + level ([Ca2 + ]i) labeled with fluo- rescence probe Fluo- 3/ AM was m easured under a laser scanning confocal microscope.Results re- vealed that Are(3- 10 0 μm ol/ L) could inhibit L- type calcium current in a concentration- depen- dent manner and the value of IC50 was33.73μm ol/ L (n=5 ) .In the absence of extracellular calci- um,the resting levels of[Ca2 + ]i was not affected by Are(n=6 ,P>0 .0 5 ) ,but pretreatment with Are(30 μmol/ L) could significantly inhibit the[Ca2 + ]i elevation induced by caffeine(10 m mol/ L,n=6 ,P<0 .0 1) .It was concluded that Are could inhibit not only calcium influx through L- type calcium channel but also calcium release from sarcoplasm ic reticulum.
