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ACTB基因在肝细胞癌中的研究进展
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作者 冯润林 梁雪婷 +5 位作者 王一芃 汪毓寅 陆文杰 张佳欣 王虹粤 李永盛 《现代医药卫生》 2025年第11期2532-2536,共5页
旨在深入探讨β-肌动蛋白(ACTB)在肝细胞癌(HCC)发生与进展中的多重作用机制,以期为临床诊疗和靶向治疗提供新的思路。首先,基于国内外HCC流行病学数据,强调了该病高发、早诊困难与治疗选择有限的现实困境,指出细胞骨架蛋白在肿瘤生物... 旨在深入探讨β-肌动蛋白(ACTB)在肝细胞癌(HCC)发生与进展中的多重作用机制,以期为临床诊疗和靶向治疗提供新的思路。首先,基于国内外HCC流行病学数据,强调了该病高发、早诊困难与治疗选择有限的现实困境,指出细胞骨架蛋白在肿瘤生物学中尚未被充分挖掘的潜力;随后,通过整合大规模转录组和单细胞测序数据,系统揭示了ACTB在HCC组织和细胞模型中异常上调的表达模式,并进一步证明其与肿瘤细胞增殖加速、迁移侵袭性增强及对常规化疗耐药性的提高密切相关;在分子层面,阐明了ACTB通过重构肌动蛋白网络,并激活Wnt/β-catenin、PI3K/AKT等关键信号通路,协同调控上皮-间质转化、细胞骨架动力学及肿瘤微环境免疫逃逸;最后,该文针对ACTB的小分子抑制剂、特异性抗体及RNA干扰策略的发展进程,分析了药物递送和肿瘤特异性靶向的挑战,并提出结合患者个体化基因表达谱、运用高通量功能筛选与精准医疗平台的联合诊疗新模式,以期显著提升HCC患者的预后和生活质量。 展开更多
关键词 actb基因 肝细胞癌 肿瘤 治疗策略 综述
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A Survey on the Existence of Harmonic Metrics on Vector Bundles
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作者 SHEN Zhenghan ZHANG Xi 《数学进展》 北大核心 2025年第2期390-404,共15页
In this paper,we give a survey on the existence of Hermitian-Einstein metrics and harmonic metrics.
关键词 Hermitian-Einstein metric harmonic metric holomorphic vector bundle non-Hermitian Yang-Mills bundle
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A VECTOR BUNDLE VALUED MIXED HARD LEFSCHETZ THEOREM
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作者 Zeng CHEN Guanxiang WANG 《Acta Mathematica Scientia》 2025年第2期514-524,共11页
In this paper,we obtain a vector bundle valued mixed hard Lefschetz theorem.The argument is mainly based on the works of Tien-Cuong Dinh and Viet-Anh Nguyen.
关键词 hard Lefschetz theorem holomorphic vector bundle Hermitian fat vector bundle
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Application Value of Bundle Management in Safe Nursing Care for Patients with Autoimmune Encephalitis
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作者 Ying Cui 《Journal of Clinical and Nursing Research》 2025年第4期149-154,共6页
Objective:To explore the application effect of bundle management in the safe nursing of patients with autoimmune encephalitis.Methods:Seventy-five patients with autoimmune encephalitis who met the inclusion criteria i... Objective:To explore the application effect of bundle management in the safe nursing of patients with autoimmune encephalitis.Methods:Seventy-five patients with autoimmune encephalitis who met the inclusion criteria in our hospital from January 2024 to June 2024 were randomly divided into two groups:an observation group of 39 patients and a control group of 36 patients.The control group received routine nursing care,while the observation group implemented a bundle management strategy based on routine nursing care.Safety nursing outcomes,clinical symptom improvement time,hospital stay,and neurologic function recovery were observed in both groups.Results:The incidence of adverse events in the observation group was 12.82%,significantly lower than the 33.33%in the control group,with a statistically significant difference(P<0.05).There was no statistically significant difference in restraint usage and ICU transfer rates between the two groups(P>0.05).The clinical symptom improvement time,hospital stay,and neurologic function recovery in the observation group were significantly better than those in the control group,with a statistically significant difference(P<0.05).Conclusion:Through the bundle management model,effective connections can be ensured in various aspects of treatment and rehabilitation for patients with autoimmune encephalitis,providing patients with comprehensive and multi-level nursing services and improving their overall satisfaction and treatment effectiveness. 展开更多
关键词 bundle management Autoimmune Encephalitis Safe nursing Clinical effect
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EXISTENCE OF SOME SPECIAL CONFORMALLY-KAHLER METRICS ON CERTAIN CP1 BUNDLES
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作者 Jing CHEN Daniel GUAN 《Acta Mathematica Scientia》 2025年第2期525-539,共15页
In this paper,we prove that for certain fiber bundles there is a k-Futaki-Ono conformally Kahler metric related to a metric in any given Kahler class for any k≥2.
关键词 Hermitian metrics conformally-Kahler metrics complex manifolds scalar curvature fiber bundle almost homogeneous manifolds
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Application of Bundle Nursing Strategy in the Maintenance of Difficult Blood Vessels for Long-term Hemodialysis Patients
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作者 Yelin Wang Liangmei Fei 《Journal of Clinical and Nursing Research》 2025年第5期297-303,共7页
Objective:To study the application effect of bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients.Methods:A prospective study was conducted on 40 long-term hemodial... Objective:To study the application effect of bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients.Methods:A prospective study was conducted on 40 long-term hemodialysis patients admitted from January 2024 to January 2025.The patients were randomly divided into a control group(20 cases)and an observation group(20 cases)using computer random sampling.The control group received routine nursing for difficult blood vessels,while the observation group received a bundle nursing strategy for difficult blood vessels.The self-care ability,complication rate,and nursing satisfaction were compared between the two groups.Results:After nursing,the self-care ability score of the observation group was higher than that of the control group(P<0.05).The complication rate in the observation group was lower than that in the control group(P<0.05).The nursing satisfaction in the observation group was higher than that in the control group(P<0.05).Conclusion:The application of the bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients is beneficial for improving self-care ability,reducing complications,and increasing patient satisfaction with nursing services. 展开更多
关键词 Long-term hemodialysis Difficult blood vessels Routine nursing bundle nursing strategy
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The Clinical Application Value of Bundled Nursing Care in Postoperative Recovery of Lung Cancer Patients
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作者 Cuifang Liu Yaqian Li +3 位作者 Kun Wang Pei Liu Hongyu Liang Xiaoxue Huang 《Journal of Clinical and Nursing Research》 2025年第4期183-189,共7页
Objective:To investigate the clinical application value of bundled nursing care in postoperative recovery of lung cancer patients.Methods:Eighty lung cancer patients who underwent surgical treatment from October 2022 ... Objective:To investigate the clinical application value of bundled nursing care in postoperative recovery of lung cancer patients.Methods:Eighty lung cancer patients who underwent surgical treatment from October 2022 to May 2024 were selected as the study subjects.Their clinical data were retrospectively analyzed and grouped by nursing methods.The bundled nursing care group(n=40)received bundled nursing care,while the conventional nursing care group(n=40)received routine nursing care.Lung function,immune function,complication rate,pain level,exercise tolerance,and quality of life were compared between the two groups.Results:Before nursing,there were no statistically significant differences in lung function,immune function,pain level,exercise tolerance,and quality of life between the bundled nursing care group and the conventional nursing care group(P>0.05).After nursing,both groups showed improvement in lung function,immune function,pain level,exercise tolerance,and quality of life,but the bundled nursing care group had better results and a lower complication rate,with statistical significance(P<0.05).Conclusion:The bundled nursing care has a higher clinical application value in postoperative recovery of lung cancer patients and is worthy of widespread clinical use. 展开更多
关键词 bundled nursing care Lung cancer Lung function Immune function Pain level Exercise tolerance
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Development of a capillary bundle evaporation advanced mathematical modeling for 1,2-propylene glycol-glycerin mixtures in porous media
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作者 Bingbing Li Jiantong Li +3 位作者 Side Ren Shuo Gu Zhanjian Liu Liyan Liu 《Chinese Journal of Chemical Engineering》 2025年第4期261-273,共13页
Porous liquid-conducting micro-heat exchangers have garnered considerable attention for their role in efficient heat dissipation in small electronic devices.This demand highlights the need for advanced mathematical mo... Porous liquid-conducting micro-heat exchangers have garnered considerable attention for their role in efficient heat dissipation in small electronic devices.This demand highlights the need for advanced mathematical models to optimize the selection of mixed heat exchange media and equipment design.A capillary bundle evaporation model for porous liquid-conducting media was developed based on the conjugate mass transfer evaporation rate prediction model of a single capillary tube,supplemented by mercury injection experimental data.Theoretical and experimental comparisons were conducted using 1,2-propanediol-glycerol(PG-VG)mixtures at molar ratios of 1:9,3:7,5:5,and 7:3 at 120,150,and 180℃.The Jouyban-Acree model was implemented to enhance the evaporation rate predictions.For the 7:3 PG-VG mixture at 180℃under the experimental conditions of the thermal medium,the model's error reduced from 16.75%to 10.84%post-correction.Overall,the mean relative error decreased from 11.76%to 5.98%after correction. 展开更多
关键词 Evaporation in porous media Capillary bundle model 1 2-propylene glycol-glycerin Evaporation rate
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Efficacy of single-lumen and double-lumen peripherally inserted central catheters in patients undergoing digestive surgery within bundled care contexts
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作者 Xiao-Hui Ye Rong-Hong Cui +3 位作者 Lei Xu Mei-Jun Wang Ling-Rong Ye Ming Jiang 《World Journal of Gastrointestinal Surgery》 2025年第9期215-226,共12页
BACKGROUND Peripherally inserted central catheters(PICCs)are crucial for patients requiring long-term intravenous therapy,especially within digestive surgery under bundled care protocols.AIM To evaluate and compare th... BACKGROUND Peripherally inserted central catheters(PICCs)are crucial for patients requiring long-term intravenous therapy,especially within digestive surgery under bundled care protocols.AIM To evaluate and compare the efficacy,safety,and patient-reported outcomes of single-lumen vs double-lumen PICCs among patients undergoing digestive surgery within a structured bundled care framework.METHODS This retrospective cohort study analyzed data from 249 patients who underwent digestive surgery and utilized either single-lumen(n=117)or double-lumen(n=132)PICCs between January 2021 and June 2024.Clinical outcomes,patient satisfaction,catheterization duration,and complication rates were compared using statistical analysis via SPSS(version 29.0).The bundled care protocol was consistently applied,focusing on standardized procedures,staff training,and patient support.RESULTS Single-lumen PICCs were associated with a significantly lower thrombosis rate(0.85%)than double-lumen PICCs(6.82%,P=0.039).The single-lumen group experienced shorter catheterization durations(12.5±3.14 days vs 13.6±4.50 days,P=0.025)and higher successful infusion rates(92.7%±5.32%vs 90.4%±6.60%,P=0.003).This group also reported higher comfort scores(8.40±1.20 vs 7.90±1.50,P=0.004)and lower pain levels(2.90±0.70 vs 3.20±0.80,P=0.002).Aside from thrombosis,complication rates showed no significant difference between the groups.CONCLUSION Within bundled care context,single-lumen PICCs demonstrated advantages in reducing thrombosis risk,procedural efficiency,patient comfort,and satisfaction compared with double-lumen PICCs.The findings underscore the importance of considering patient-specific needs and clinical scenarios in catheter choice. 展开更多
关键词 Single-lumen peripherally inserted central catheter Double-lumen peripherally inserted central catheter Digestive surgery bundled care Patient satisfaction THROMBOSIS
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Left bundle branch pacing cardiac resynchronization therapy vs biventricular pacing cardiac resynchronization therapy–time to write a requiem for biventricular pacing-cardiac resynchronization therapy
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作者 Akshyaya Pradhan Daljeet Saggu Monika Bhandari 《World Journal of Cardiology》 2025年第2期118-124,共7页
Cardiac resynchronization therapy(CRT)reduces heart failure(HF)hospitaliz-ations and all-cause mortality in patients with HF with reduced ejection fraction with left bundle branch(LBB)block.Biventricular pacing(BVP)is... Cardiac resynchronization therapy(CRT)reduces heart failure(HF)hospitaliz-ations and all-cause mortality in patients with HF with reduced ejection fraction with left bundle branch(LBB)block.Biventricular pacing(BVP)is considered the gold standard for achieving CRT;however,approximately 30%–40%of patients do not respond to BVP-CRT.Recent studies have demonstrated that LBB pacing(LBBP)produces remarkable results in CRT.In this meta-analysis,LBBP-CRT showed better outcomes than conventional BVP-CRT,including greater QRS duration reduction and left ventricular ejection fraction improvement,along with consistently lower pacing thresholds on follow-up.Additionally,there was a grea-ter reduction in New York Heart Association class and brain natriuretic peptide levels.This study contributes to the growing body of encouraging data on LBBP-CRT from recent years.With ongoing technological advancements and increasing operator expertise,the day may not be far when LBBP-CRT becomes the standard of care rather than the exception. 展开更多
关键词 Heart failure Left bundle branch block area pacing Narrow QRS New York Heart Association class Left ventricular ejection fraction
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横掠扭曲/直扁管组合管束的流动与传热特性研究
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作者 高全福 王元涛 +1 位作者 孙恺 宋克伟 《华中科技大学学报(自然科学版)》 北大核心 2026年第1期135-141,共7页
针对圆管管束式换热器流动阻力大的问题,提出了几种扭曲扁管与直扁管以不同方式排列的组合管束.在雷诺数(Re)2500~15000范围内,数值分析了不同组合管束对换热器流动特性和换热性能的影响.对比分析了扭曲扁管管束、扭曲/直扁管组合管束... 针对圆管管束式换热器流动阻力大的问题,提出了几种扭曲扁管与直扁管以不同方式排列的组合管束.在雷诺数(Re)2500~15000范围内,数值分析了不同组合管束对换热器流动特性和换热性能的影响.对比分析了扭曲扁管管束、扭曲/直扁管组合管束和圆管管束换热器的流场和温度局部分布,以及努塞尔数(Nu)、欧拉数(Eu)和综合换热评价系数(PEC)等平均特性.结果表明:扭曲扁管管束Nu和Eu均最大,Nu相比于圆管管束提升了10%~18.2%,Eu则增大了14.2%~18.1%,使得扭曲扁管管束的综合性能相对较小;相比于圆管管束,前三排为扭曲扁管最后一排为直扁管组合的Nu最大提升了12.77%,前两排为直扁管后两排为扭曲扁管组合的Eu数最大降低了28.13%.前两排为扭曲扁管后两排为直扁管的组合因换热大于圆管管束而阻力小于圆管管束,使得综合性能PEC最大提升了16.52%.组合管束能够在有效降低泵功消耗的同时,实现更优的综合换热效果. 展开更多
关键词 管束式换热器 横掠 扭曲扁管 组合管束 强化换热
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基于循证的集束化降温管理策略在热射病病人护理中的应用效果
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作者 赵霞 王群 +3 位作者 左霞 陈月莉 陆明峰 谈定玉 《循证护理》 2026年第3期487-493,共7页
目的:探讨基于循证的集束化降温管理策略在热射病病人中的应用效果。方法:选取2020年7月—2023年9月我院急诊科收治的热射病病人作为研究对象,其中2020年7月—2021年9月收治的46例病人作为对照组,2022年7月—2023年9月收治的48例病人作... 目的:探讨基于循证的集束化降温管理策略在热射病病人中的应用效果。方法:选取2020年7月—2023年9月我院急诊科收治的热射病病人作为研究对象,其中2020年7月—2021年9月收治的46例病人作为对照组,2022年7月—2023年9月收治的48例病人作为观察组。对照组采用传统降温措施,观察组在对照组基础上采用基于循证的集束化降温管理策略,该策略汇总为环境降温、浅表血管处冰敷、冰帽冷敷、乙醇擦浴配合风扇降温、液体降温、体温监测及管理6个主题,共18条证据。比较两组病人降温速度、降温达标率、格拉斯哥昏迷评分(GCS)和病死率。结果:干预后,观察组核心体温降至39.0℃时间、降至38.5℃时间均明显低于对照组(均P<0.05);观察组核心体温30 min内降至39.0℃及以下、2 h内降至38.5℃及以下的比例明显高于对照组(均P<0.05);观察组治疗24 h后GCS评分明显高于对照组(P<0.001);观察组病死率明显低于对照组(P<0.05)。结论:基于循证的集束化降温策略管理可提升热射病的临床治疗效果,并改善病人预后。 展开更多
关键词 热射病 集束化护理 循证护理 降温管理 急救
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海洋数字编排:数字何以赋能海洋治理?——基于对山东省七年数字海洋实践的案例分析
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作者 王刚 吴嘉莉 徐雅倩 《上海行政学院学报》 北大核心 2026年第1期39-52,共14页
数字赋能治理引发了学界的浓厚兴趣,海洋领域却鲜少被关注。基于山东省的案例分析,概括出数字赋能海洋治理的核心解释机制——海洋数字编排。通过分层引导的平台建构形成海洋治理数据资源池,遵循以安全为基础的数据捆绑形成海洋数据产... 数字赋能治理引发了学界的浓厚兴趣,海洋领域却鲜少被关注。基于山东省的案例分析,概括出数字赋能海洋治理的核心解释机制——海洋数字编排。通过分层引导的平台建构形成海洋治理数据资源池,遵循以安全为基础的数据捆绑形成海洋数据产品或数据服务,最终凭借需求放大和阻力减少的场景撬动实现势能转换,赋能海洋治理的数字化转型。该过程受到权限约束与监管透视的资源整合、价值释放和安全保障的动态平衡以及为应对考核不确定性从而主动兼容的多重逻辑驱动。积极主动识别“数字机遇之窗”,根据业务专长规划顶层设计;审慎平衡数字势能积累与制度惯性之间的张力,以主动兼容的思维和充分的准备应对不确定性是海洋数字编排成功实现的有效策略。 展开更多
关键词 海洋数字编排 平台建构 数据捆绑 场景撬动 数字海洋
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DTN网络中的基于Bundle协议的数据包发送算法 被引量:2
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作者 王堃 于悦 +2 位作者 张玉华 郭篁 王琳琳 《吉林大学学报(工学版)》 EI CAS CSCD 北大核心 2012年第S1期285-289,共5页
DTN传输协议引入束和分片的概念,将数据分块传输以适应经常发生链接中断的链路。研究了DTN传输层的Bundle协议和LTP(Licklider transmission protocol)协议,针对网络环境可能在短时间内剧烈变化的问题,研究了数据块的大小对传输效率的影... DTN传输协议引入束和分片的概念,将数据分块传输以适应经常发生链接中断的链路。研究了DTN传输层的Bundle协议和LTP(Licklider transmission protocol)协议,针对网络环境可能在短时间内剧烈变化的问题,研究了数据块的大小对传输效率的影响,提出了DTN网络中基于Bundle协议的数据包发送算法,动态选择最适合当前网络状况的发送策略以获得最佳的系统性能和传输效率。仿真结果表明:适当选取发送数据块的大小可以大幅度提高传输效率。 展开更多
关键词 计算机应用 容迟网络 bundle 分片 传输效率
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清单式集束化护理策略在胃肠外科术后的应用效果及对下肢深静脉血栓的影响
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作者 李敏 汪春霞 谭佳 《中国当代医药》 2026年第2期155-159,共5页
目的探讨清单式集束化护理策略在胃肠外科术后下肢深静脉血栓(DVT)预防中的应用效果。方法选取2022年1月至2023年1月南昌大学附属康复医院80例接受胃肠外科手术治疗的患者作为研究对象,按照随机数字表法分为对照组(40例)和干预组(40例)... 目的探讨清单式集束化护理策略在胃肠外科术后下肢深静脉血栓(DVT)预防中的应用效果。方法选取2022年1月至2023年1月南昌大学附属康复医院80例接受胃肠外科手术治疗的患者作为研究对象,按照随机数字表法分为对照组(40例)和干预组(40例)。对照组实施常规护理,干预组实施清单式集束化护理策略干预,两组均干预至出院。比较两组下肢DVT发生率、下肢周径、下肢深静脉血流速度、住院时间、生活质量、并发症发生率及护理满意度。结果干预组下肢DVT发生率以及并发症总发生率均低于对照组,差异有统计学意义(P<0.05)。干预组下肢周径和住院时间均短于对照组,下肢深静脉血流速度快于对照组,差异有统计学意义(P<0.05)。干预组慢性静脉功能不全生活质量量表(CIVIQ)评分均高于对照组,差异有统计学意义(P<0.05)。干预组护理满意度各项评分均高于对照组,差异有统计学意义(P<0.05)。结论清单式集束化护理策略对胃肠外科术后下肢DVT的预防效果确切,不仅能够缩短患者下肢周径,减轻下肢肿胀,延长下肢深静脉血流速度,对降低下肢DVT发生率有积极作用,还能够减少术后肺部感染、切口感染等发生概率,提高患者的生活质量,从而获得更高的护理满意度,值得在胃肠外科手术中推广应用。 展开更多
关键词 胃肠外科 下肢深静脉血栓 清单式集束化护理 生活质量
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基于Douglas-Peucker和Quick Bundles算法的水上交通模式识别 被引量:4
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作者 陈信强 徐祥龙 +3 位作者 彭静 孙洋 王梓创 阎莹 《上海海事大学学报》 北大核心 2022年第3期1-6,共6页
针对船舶航迹数据量大、数据冗余、航迹特征不明显等问题,提出一种融合道格拉斯-普克(Douglas-Peucker,DP)压缩算法和基于距离的快速捆绑包(Quick Bundles,QB)聚类算法的水上交通模式识别方法。该方法根据航迹数据特征、压缩率和压缩误... 针对船舶航迹数据量大、数据冗余、航迹特征不明显等问题,提出一种融合道格拉斯-普克(Douglas-Peucker,DP)压缩算法和基于距离的快速捆绑包(Quick Bundles,QB)聚类算法的水上交通模式识别方法。该方法根据航迹数据特征、压缩率和压缩误差等指标选择合理的压缩阈值实现大规模船舶自动识别系统(automatic identification system,AIS)数据的压缩。在此基础上,提出一种基于最小直接翻转距离的聚类指标,利用QB算法实现船舶航迹的有效聚类。实验结果表明,提出的方法既可以简化航迹聚类过程,也可准确高效地实现航迹聚类,为水上交通精细化管理与决策提供数据支撑。 展开更多
关键词 船舶航迹聚类 船舶自动识别系统(AIS) DOUGLAS-PEUCKER算法 Quick bundles算法
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QTL Analysis of Rice Peduncle Vascular Bundle System and Panicle Traits 被引量:17
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作者 滕胜 钱前 +3 位作者 曾大力 国广泰史 黄大年 朱立煌 《Acta Botanica Sinica》 CSCD 2002年第3期301-306,共6页
A double haploid (DH) population of rice (Oryza sativa L.) derived from anther culture of ZYQ8/JX17, a typical indica and japonica hybrid, was used for genetic analysis of rice peduncle vascular system and panicle tra... A double haploid (DH) population of rice (Oryza sativa L.) derived from anther culture of ZYQ8/JX17, a typical indica and japonica hybrid, was used for genetic analysis of rice peduncle vascular system and panicle traits. The number of large vascular bundles (LVB), the number of small vascular bundles (SVB) in the peduncle, and the panicle traits including the number of primary rachis branches (PRB), the number of spikelets per panicle (SNP), peduncle top diameter (PTD), and panicle length (PL) were investigated in the parents and DH lines. The quantitative trait loci (QTLs) for each trait were analyzed based on the constructed molecular linkage map of this population. Three QTLs for LVB (qLVB_1, qLVB_6 and qLVB_7) were detected on chromosomes 1, 6, and 7, respectively. Two putative QTLs for SVB (qSVB_4 and qSVB_6) were mapped on chromosomes 4 and 6 respectively. Four QTLs (qPRB_4a, qPRB_4b, qPRB_6 and qPRB_7) on chromosomes 4, 6, and 7, respectively, were detected for PRB. Three QTLs (qSPN_4a, qSPN_4b and qSPN_6) were identified on chromosomes 4 and 6, respectively, which could significantly affect SPN. Five QTLs for PTD (qPTD_2, qPTD_5, qPTD_6, qPTD_8 and qPTD_12) were identified on chromosomes 2, 5, 6, 8, and 12, respectively. Three QTLs for PL (qPL_4, qPL_6 and qPL_8) were detected on chromosomes 4, 6, and 8, respectively. Clustering of QTLs, such as qLVB_6, qSVB_6, qSNP_6, qPTD_6, and qPL_6 detected in the interval G122_G1314b on chromosome 6, was found. These results suggest that some QTLs for peduncle vascular bundle system are possibly responsible for the panicle traits. 展开更多
关键词 RICE peduncle vascular bundle panicle traits QTL analysis
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ACTB基因第4外显子遗传变异分析及其与种公牛精液品质的关系 被引量:1
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作者 岳续朋 贺洪涛 +3 位作者 张志刚 戴立胜 赵锐锋 张嘉保 《中国兽医学报》 CAS CSCD 北大核心 2017年第3期551-553,共3页
采用PCR-SSCP技术对45头种公牛的ACTB(β肌动蛋白)基因的第4外显子的单核甘酸多态性进行了检测。结果显示:在ACTB基因第4外显子上存在2种基因型,即AA型和AB型。对该多肽片段克隆测序,结果在ACTB基因第4外显子上存在G378C突变。用最小二... 采用PCR-SSCP技术对45头种公牛的ACTB(β肌动蛋白)基因的第4外显子的单核甘酸多态性进行了检测。结果显示:在ACTB基因第4外显子上存在2种基因型,即AA型和AB型。对该多肽片段克隆测序,结果在ACTB基因第4外显子上存在G378C突变。用最小二乘法分析ACTB基因的第4外显子不同基因型与精液品质的相关性,发现ACTB基因第4外显子AA型精子浓度、鲜精畸形率以及冻精畸形率显著高于AB型(P<0.05),AA型鲜精活力、鲜精活精子比率极显著高于AB型(P<0.01),AA型冻精活精子比率显著低于AB型(P<0.05)。 展开更多
关键词 actb基因 PCR SSCP 单核甘酸多态性(SNP)
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贵州香猪ACTB基因的原核表达 被引量:1
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作者 曹龙凯 郭春华 艾丹 《西南民族大学学报(自然科学版)》 CAS 2012年第1期73-76,共4页
以贵州香猪ACTB基因的RNA序列为模板,运用PCR技术成功扩增出香猪ACTB基因的CDS区,将其插入到pET-32a原核表达载体,构建重组表达质粒pET-32a-ACTB并转化至BL21(DE3)宿主菌中,IPTG诱导表达,经SDS-PAGE检测,pET-32a-ACTB在宿主菌BL21(DE3)... 以贵州香猪ACTB基因的RNA序列为模板,运用PCR技术成功扩增出香猪ACTB基因的CDS区,将其插入到pET-32a原核表达载体,构建重组表达质粒pET-32a-ACTB并转化至BL21(DE3)宿主菌中,IPTG诱导表达,经SDS-PAGE检测,pET-32a-ACTB在宿主菌BL21(DE3)中表达出融合蛋白,结果分析表明ACTB基因在宿主菌中获得了高效表达,表达出的蛋白大小与预期结果相符. 展开更多
关键词 贵州香猪 actb基因 原核表达
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燃料组件边角栅元临界热流密度试验研究
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作者 王喆 《科技创新与应用》 2026年第2期7-10,14,共5页
为深入探究燃料组件边角栅元的临界热流密度(CHF)特性,以及典型栅元和冷壁栅元试验所获CHF关系式对边角栅元的适用性,在中广核研究院大型热工试验装置(LATHY)上开展边角栅元CHF试验。试验装置由25根5×5排列的轴向均匀加热棒构成,... 为深入探究燃料组件边角栅元的临界热流密度(CHF)特性,以及典型栅元和冷壁栅元试验所获CHF关系式对边角栅元的适用性,在中广核研究院大型热工试验装置(LATHY)上开展边角栅元CHF试验。试验装置由25根5×5排列的轴向均匀加热棒构成,其中外围19根为低功率冷棒,中心6根为高功率热棒,功率比1∶0.8,加热棒内壁沿轴向布置热电偶监测温度。试验条件覆盖的压力范围为10~17 MPa(a),入口温度范围为140~300℃,入口质量流量范围为1 300~4 100 kg/(m^(2)·s),共获取10个数据。对比分析设计与实际工况运行参数后,发现试验参数偏差控制在小范围内,表明试验高度的可控性。计算结果表明,CHF实测值与预测值偏差控制在20%以内,且CHF预测位置与实测位置一致,验证预测模型准确性。典型栅元和冷壁栅元试验所得CHF关系式基本适用于边角栅元。 展开更多
关键词 边角栅元 偏离泡核沸腾比 临界热流密度 棒束试验 燃料组件
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