In order to investigate the origin of neointimal smooth muscle cells in transplant arterio- sclerosis in rat aortic allograft, sex-mismatched bone marrow transplantation was performed from male Wistar rats to female W...In order to investigate the origin of neointimal smooth muscle cells in transplant arterio- sclerosis in rat aortic allograft, sex-mismatched bone marrow transplantation was performed from male Wistar rats to female Wistar rats. Four weeks after transplantation, the aortic transplant model was established by means of micro-surgery in rats. The recipients were divided into 4 groups: female Wistar-female Wistar aortic isografts, female SD-female Wistar aortic allografts, male SD-male Wis- tar aortic allografts, female SD-chimera Wistar aortic allografts. Eight weeks after transplantation, aortic grafts were removed at autopsy and processed for histological evaluation and immunohisto- chemistry. The results indicated that excessive accumulation of α-SMA-positive smooth muscle cells resulted in significant neointima formation and vascular lumen stricture in rat aortic allografts. Neointima assay revealed that the neointimal area and NIA/MA ratio of transplanted artery were sig- nificantly increased in all of aortic allograft groups as compared with those in aortic isograft group (P<0.01). Neointimal smooth muscle cells were harvested from cryostat sections of aortic allograft by microdissection method. The Sry gene-specific PCR was performed, and the result showed that a dis- tinct DNA band of 225 bp emerged in the male-male aortic allograft group and chimera aortic al- lograft group respectively, but not in the female-female aortic allograft group. It was suggested that recipient bone-marrow cells, as the origin of neointimal smooth muscle cells, contributed to the pathological neointimal hyperplasia of aortic allograft and transplant arteriosclerosis.展开更多
Objectives: To 1) explore the planar scintigraphic findings in asymmetry patients caused by unilateral condylar hyperplasia (UCH) or asymmetric mandibular hyperplasia (AMH);2) develop a local agecondylar activity refe...Objectives: To 1) explore the planar scintigraphic findings in asymmetry patients caused by unilateral condylar hyperplasia (UCH) or asymmetric mandibular hyperplasia (AMH);2) develop a local agecondylar activity reference norm;and 3) check the accuracy of the new norm and compare with other analytical methods. Material and Method: Chinese patients with mandibular asymmetry and scintigraphic findings available were recruited. Clinical, radiographic records and scintigraphic condylar activity ratio (CAR) were studied. Regression analysis was performed to quantify the relationship between age and CAR to develop a new norm. The sensitivity of condylar activity assessment using a) traditional norm;b) new norm;and c) percentile difference was compared. In patients with serial data available, longitudinal analysis of the scintigraphic changes were checked. Result: 109 patients were eligible for the study. Significant difference in CAR was noted between UCH and AMH patients. Linear relationship was observed between age and CAR. A new norm of the age-CAR was established, which showed improved sensitivity in condylar activity prediction in UCH and AMH when compared with traditional norm. Relative to percentile difference, the sensitivity of new norm was lower in AMH but not in UCH patients. Serial analysis revealed gradual decline in CAR with minimal change in percentile difference. Conclusion and Clinical Relevance: There is difference in scintigraphic condylar activity between UCH and AMH patients. A norm of age-CAR relationship was established, which showed improved sensitivity in condylar activity prediction in UCH patients. Further study is required to confirm the role of scintigraphy in AMH.展开更多
目的探讨老年退行性膝关节炎患者采用抗骨质增生丸联合玻璃酸钠腔内注射治疗的临床效果。方法研究对象选取60例2019年1月—2024年5月江陵县中医医院收治的老年退行性膝关节炎患者,将其分为各30例的两组,分组依据为不同治疗方法。常规组...目的探讨老年退行性膝关节炎患者采用抗骨质增生丸联合玻璃酸钠腔内注射治疗的临床效果。方法研究对象选取60例2019年1月—2024年5月江陵县中医医院收治的老年退行性膝关节炎患者,将其分为各30例的两组,分组依据为不同治疗方法。常规组采用玻璃酸钠腔内注射治疗,探究组采用抗骨质增生丸联合玻璃酸钠腔内注射治疗。比较两组膝关节功能相关评分、关节活动度、症状积分、并发症发生率。结果与常规组相比,探究组治疗后西安大略和麦克马斯特大学骨关节炎指数和美国特种外科医院膝关节评分系统评分均更优,关节活动度更高,膝关节疼痛、关节僵硬、关节肿胀、关节畸形评分均更低,差异均有统计学意义(P均<0.05)。并发症发生率两组相比,探究组低于常规组[0 vs 20.00%(6/30)],差异有统计学意义(P=0.031)。结论老年退行性膝关节炎患者采用抗骨质增生丸与玻璃酸钠腔内注射的治疗方案,能有效提升患者的膝关节功能和关节活动度,降低症状积分和并发症发生率。展开更多
骨纤维异常增殖症（fibrous dysplasia of bone,FDB）,目前临床关于其发病机制尚不明确,多数学者认为是由于原始间叶组织出现异常发育,骨内纤维组织出现异常增生引起,约占骨肿瘤的2.5%和非恶性骨肿瘤的7%[1-2]。其生长特点为病骨区畸形...骨纤维异常增殖症（fibrous dysplasia of bone,FDB）,目前临床关于其发病机制尚不明确,多数学者认为是由于原始间叶组织出现异常发育,骨内纤维组织出现异常增生引起,约占骨肿瘤的2.5%和非恶性骨肿瘤的7%[1-2]。其生长特点为病骨区畸形肿胀,可广泛侵入鼻窦、眼眶及颅前窝底,表现为鼻塞、嗅觉减退、面部不对称、眼球突出、移位、复视、视力障碍和张口困难等。展开更多
基金grants from the National Natural Sciences Foundation of China (No. 30271242, 30371396)
文摘In order to investigate the origin of neointimal smooth muscle cells in transplant arterio- sclerosis in rat aortic allograft, sex-mismatched bone marrow transplantation was performed from male Wistar rats to female Wistar rats. Four weeks after transplantation, the aortic transplant model was established by means of micro-surgery in rats. The recipients were divided into 4 groups: female Wistar-female Wistar aortic isografts, female SD-female Wistar aortic allografts, male SD-male Wis- tar aortic allografts, female SD-chimera Wistar aortic allografts. Eight weeks after transplantation, aortic grafts were removed at autopsy and processed for histological evaluation and immunohisto- chemistry. The results indicated that excessive accumulation of α-SMA-positive smooth muscle cells resulted in significant neointima formation and vascular lumen stricture in rat aortic allografts. Neointima assay revealed that the neointimal area and NIA/MA ratio of transplanted artery were sig- nificantly increased in all of aortic allograft groups as compared with those in aortic isograft group (P<0.01). Neointimal smooth muscle cells were harvested from cryostat sections of aortic allograft by microdissection method. The Sry gene-specific PCR was performed, and the result showed that a dis- tinct DNA band of 225 bp emerged in the male-male aortic allograft group and chimera aortic al- lograft group respectively, but not in the female-female aortic allograft group. It was suggested that recipient bone-marrow cells, as the origin of neointimal smooth muscle cells, contributed to the pathological neointimal hyperplasia of aortic allograft and transplant arteriosclerosis.
文摘Objectives: To 1) explore the planar scintigraphic findings in asymmetry patients caused by unilateral condylar hyperplasia (UCH) or asymmetric mandibular hyperplasia (AMH);2) develop a local agecondylar activity reference norm;and 3) check the accuracy of the new norm and compare with other analytical methods. Material and Method: Chinese patients with mandibular asymmetry and scintigraphic findings available were recruited. Clinical, radiographic records and scintigraphic condylar activity ratio (CAR) were studied. Regression analysis was performed to quantify the relationship between age and CAR to develop a new norm. The sensitivity of condylar activity assessment using a) traditional norm;b) new norm;and c) percentile difference was compared. In patients with serial data available, longitudinal analysis of the scintigraphic changes were checked. Result: 109 patients were eligible for the study. Significant difference in CAR was noted between UCH and AMH patients. Linear relationship was observed between age and CAR. A new norm of the age-CAR was established, which showed improved sensitivity in condylar activity prediction in UCH and AMH when compared with traditional norm. Relative to percentile difference, the sensitivity of new norm was lower in AMH but not in UCH patients. Serial analysis revealed gradual decline in CAR with minimal change in percentile difference. Conclusion and Clinical Relevance: There is difference in scintigraphic condylar activity between UCH and AMH patients. A norm of age-CAR relationship was established, which showed improved sensitivity in condylar activity prediction in UCH patients. Further study is required to confirm the role of scintigraphy in AMH.
文摘目的探讨老年退行性膝关节炎患者采用抗骨质增生丸联合玻璃酸钠腔内注射治疗的临床效果。方法研究对象选取60例2019年1月—2024年5月江陵县中医医院收治的老年退行性膝关节炎患者,将其分为各30例的两组,分组依据为不同治疗方法。常规组采用玻璃酸钠腔内注射治疗,探究组采用抗骨质增生丸联合玻璃酸钠腔内注射治疗。比较两组膝关节功能相关评分、关节活动度、症状积分、并发症发生率。结果与常规组相比,探究组治疗后西安大略和麦克马斯特大学骨关节炎指数和美国特种外科医院膝关节评分系统评分均更优,关节活动度更高,膝关节疼痛、关节僵硬、关节肿胀、关节畸形评分均更低,差异均有统计学意义(P均<0.05)。并发症发生率两组相比,探究组低于常规组[0 vs 20.00%(6/30)],差异有统计学意义(P=0.031)。结论老年退行性膝关节炎患者采用抗骨质增生丸与玻璃酸钠腔内注射的治疗方案,能有效提升患者的膝关节功能和关节活动度,降低症状积分和并发症发生率。
文摘骨纤维异常增殖症（fibrous dysplasia of bone,FDB）,目前临床关于其发病机制尚不明确,多数学者认为是由于原始间叶组织出现异常发育,骨内纤维组织出现异常增生引起,约占骨肿瘤的2.5%和非恶性骨肿瘤的7%[1-2]。其生长特点为病骨区畸形肿胀,可广泛侵入鼻窦、眼眶及颅前窝底,表现为鼻塞、嗅觉减退、面部不对称、眼球突出、移位、复视、视力障碍和张口困难等。
