Pyrola atropurpurea Franch is an important annual herbaceous plant.Few genomic analyses have been conducted on this plant,and chloroplast genome research will enrich its genomics basis.This study is based on high-thro...Pyrola atropurpurea Franch is an important annual herbaceous plant.Few genomic analyses have been conducted on this plant,and chloroplast genome research will enrich its genomics basis.This study is based on high-throughput sequencing technology and Bioinformatics methods to obtain the sequence,structure,and other characteristics of the P.atropurpurea chloroplast genome.The result showed that the chloroplast genome of P.atropurpurea has a double-stranded circular structure with a total length of 172,535 bp and a typical four-segment structure.The genome has annotated a total of 132 functional genes,including 43 tRNAs,8 rRNAs,76 protein-coding genes,and 5 pseudo-genes.In total,358 SSR loci were checked out,mainly composed of mononucleotide and trinucleotide repeat.There are three types of scattered repetitive sequences,totaling 4223,including 2452 forward repeats,1763 palindrome repeats,and eight reverse repeats.The optimal codon usage frequency is relatively high with AT usage preference in this genome.Chloroplast genome comparative analysis in the family Ericaceae shows that the overall sequence is more complex,and there are more variations in the gene interval region.The collinearity analysis indicated that there is a complex rearrangement of species between different genera in Ericaceae.The selection pressure analysis showed that the protein-encoding genes rpl33 and rps16 were positively selected among the seven medicinal plants in Ericaceae.The maximum likelihood tree shows that the genetic relationship among P.atropurpurea,Pyrola rotundifolia,and Chimaphila japonica is relatively close.Therefore,an important data basis was provided for species identification,genetic diversity,and phylogenetic studies of P.atropurpurea and even this genus of plants.展开更多
[Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer（ITS region）of rDNA.[Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DN...[Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer（ITS region）of rDNA.[Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence.[Result]The homology of ITS region between B.atropurpurea and Porphyra oligospermatangia was 75%,which was 79% between B.atropurpurea and P.yezoensis.[Conclusion]Compared with other genes,the ITS sequence had a greater evolution rate.The differences in classification and distribution resulted in the sequence diversity.展开更多
[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local v...[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local varieties of Morus atropurpurea Roxb.was analyzed by ISSR molecular marker technique.The genetic relationship among these local varieties was researched by UPGMA method based on the genetic similarity coefficient.[Result] 128 bands were amplified from the total DNA of 64 local varieties using 13 ISSR primers,of which 109 bands accounting for 85.15% were polymorphic.It meant that there was rich genetic diversity among the local varieties tested.The genetic similarity coefficients among 64 local varieties were relatively high with a range of 0.500 0-0.929 7.In addition,64 local varieties were divided into two categories and the second could be further divided into 10 subcategories.It was suggested that the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.based on ISSR marker analysis has a certain correlation with geographical distribution.[Conclusion] ISSR marker technology was suitable for evaluating genetic relationship and genetic diversity of local varieties of Morus atropurpurea Roxb.in Pearl River Basin,and could provide scientific basis for DNA fingerprinting and identification of varieties of Morus atropurpurea Roxb.展开更多
OBJECTIVE To investigate the effect of methanolic extract of Scurrula atropurpurea on systolic blood pressure and serum angiotensinⅡ concentration in deoxycorticosterone acetate-salt(DOCA-salt)hypertensive rats.METHO...OBJECTIVE To investigate the effect of methanolic extract of Scurrula atropurpurea on systolic blood pressure and serum angiotensinⅡ concentration in deoxycorticosterone acetate-salt(DOCA-salt)hypertensive rats.METHODS Twenty-five male Wistar rats were divided into normal control group and four hypertensive groups by DOCA-salt for 6 weeks.Further,three hypertensive groups were orally administered with methanolic extract of Scurrula atropurpurea at a doses of 50,100 and 200mg·kg-1,respectively for 6 weeks and one hypertensive group without extract as positive control.At the end of research systolic blood pressure were measured by tail cuff methods.Serum angiotensinⅡ were measured by ELISA.RESULTS The systolic blood pressure increased significantly in the positive control hypertensive group compared to the normal control group(P<0.05).Administration of methanolic extract of Scurrula atropurpurea 50,100 and 200mg·kg-1 significantly decreased systolic blood pressure,respectively compared to the positive control hypertensive group(P<0.05).There was no different angiotensinⅡ consentration between positive control hypertensive group and normal group,while methanolic extract of Scurrula atropurpurea 50mg·kg-1 caused decreased angiotensinⅡ conscentration significantly compared to positive control hypertensive group(P<0.05).CONCLUSION Methanolic extract of Scurrula atropurpurea produced antihypertensive effect and decreasing angiotensinⅡ concentration at 50mg·kg-1 in hypertensive rat.Beside this,the concentration of angiotensinⅡ couldn′t increase in DOCA-salt hypertensive rats.展开更多
Smilacina atropurpurea(family Convallariaceae)is a perennial herb used medicinally for the trea tment of lung ailments,rheumatism,menstrual disorders,and cuts and bruises,by the local people of its growing areas in so...Smilacina atropurpurea(family Convallariaceae)is a perennial herb used medicinally for the trea tment of lung ailments,rheumatism,menstrual disorders,and cuts and bruises,by the local people of its growing areas in southwest China.The tender aerial part has also been used as a wild vegetable by Lisu,Naxi and Tibetan people.Chemical analysis of the rhizomes of this plant has allowed us to isolate two new minor steroidal tetraglycosides,specifically atropurosides H(1) and I(2),by repeated column chromatography over silica gel and reversed phase silica.The structures were determined to be(25R,S)-12β-hydroxy-spirost-5-ene-3β-yl-O-β-D-glucopyranosy1-(1→2)-[β-D-xylopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(1)and(25S)-5α-spirost-17α-hydroxy-3β-yl-O-β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopy-ranosyl-(1→4)-β-D-galacto pyranoside(2),respectively,on the basis of detailed spectroscopic analysis including ID and 2D NM R techniques.展开更多
【目的】针对桑树形态倍性鉴定及品种间鉴别问题，研究广东桑不同倍性及品种之间孢粉学上的异同，为桑树多倍体育种提供科学依据。【方法】利用扫描电镜对广东桑10个不同倍性品种花粉的形态、大小、萌发孔、外壁纹饰等孢粉学特性进行观...【目的】针对桑树形态倍性鉴定及品种间鉴别问题，研究广东桑不同倍性及品种之间孢粉学上的异同，为桑树多倍体育种提供科学依据。【方法】利用扫描电镜对广东桑10个不同倍性品种花粉的形态、大小、萌发孔、外壁纹饰等孢粉学特性进行观察，并运用图片分析结合数理统计的方法对其花粉形态进行研究。【结果】供试广东桑不同品种的花粉微观形态相近，均为小型花粉，近球形，花粉粒极轴长16.98～24.46μm，赤道轴长15.37～23.86μm，极轴长与赤道轴长的比值为1.01～1.12；供试不同品种花粉粒萌发孔1～3个，圆形，直径为2.11～3.51μm，部分花粉粒萌发孔外壁内陷形成漏斗形，按照额尔特曼 G 的 NPC（萌发器官的数目、位置及特征）分类系统属N3P3C4类型；不同倍性间广东桑花粉的赤道轴长、极轴长和萌发孔大小存在显著性差异（P ＜0.05），均表现为四倍体＞二倍体，但花粉粒的极轴/赤轴比值差异不显著（P＞0.05）；广东桑花粉外壁为分布不均的刺状突起－脑条纹复合纹饰，不同品种间花粉的表面纹饰有明显差别，根据外壁纹饰可将供试10个品种花粉分为细刺颗粒状突起－细条纹纹饰、细刺颗粒状突起－粗条纹纹饰、粗刺颗粒状突起－细条纹纹饰和粗刺颗粒状突起－粗条纹纹饰4种类型，花粉外壁纹饰体现了不同品种的遗传多样性和遗传分化；不同倍性间广东桑花粉表面的刺状突起密度及大小差异不显著（P＞0.05）；供试四倍体广东桑品种花粉存在少数败育花粉粒，表现为干瘪而不充实，外形异常。【结论】供试广东桑不同倍性及品种间花粉形态特征具有差异，不同倍性间花粉粒和萌发孔大小存在差异，不同品种间花粉的表面纹饰存在明显差别，初步认为花粉的亚显微结构特征可作为广东桑倍性鉴定及品种间鉴别的重要标准之一。展开更多
基金supported by the Education Reform Program of Jiangxi Provincial Department of Education(JXJG-22-23-3,JXJG-23-23-5)the“Biology and Medicine”Discipline Construction Project of Nanchang NormalUniversity(100/20149)+2 种基金Jiangxi Province Key Laboratory of Oil Crops Biology(YLKFKT202203)the Education Reform Program of Nanchang Normal University(NSJG-21-25)Nanchang Key Laboratory of Comprehensive Research and Development of Brasenia schreberi(32060078).
文摘Pyrola atropurpurea Franch is an important annual herbaceous plant.Few genomic analyses have been conducted on this plant,and chloroplast genome research will enrich its genomics basis.This study is based on high-throughput sequencing technology and Bioinformatics methods to obtain the sequence,structure,and other characteristics of the P.atropurpurea chloroplast genome.The result showed that the chloroplast genome of P.atropurpurea has a double-stranded circular structure with a total length of 172,535 bp and a typical four-segment structure.The genome has annotated a total of 132 functional genes,including 43 tRNAs,8 rRNAs,76 protein-coding genes,and 5 pseudo-genes.In total,358 SSR loci were checked out,mainly composed of mononucleotide and trinucleotide repeat.There are three types of scattered repetitive sequences,totaling 4223,including 2452 forward repeats,1763 palindrome repeats,and eight reverse repeats.The optimal codon usage frequency is relatively high with AT usage preference in this genome.Chloroplast genome comparative analysis in the family Ericaceae shows that the overall sequence is more complex,and there are more variations in the gene interval region.The collinearity analysis indicated that there is a complex rearrangement of species between different genera in Ericaceae.The selection pressure analysis showed that the protein-encoding genes rpl33 and rps16 were positively selected among the seven medicinal plants in Ericaceae.The maximum likelihood tree shows that the genetic relationship among P.atropurpurea,Pyrola rotundifolia,and Chimaphila japonica is relatively close.Therefore,an important data basis was provided for species identification,genetic diversity,and phylogenetic studies of P.atropurpurea and even this genus of plants.
基金Supported by National Science Foundation of China(30970187)~~
文摘[Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer（ITS region）of rDNA.[Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence.[Result]The homology of ITS region between B.atropurpurea and Porphyra oligospermatangia was 75%,which was 79% between B.atropurpurea and P.yezoensis.[Conclusion]Compared with other genes,the ITS sequence had a greater evolution rate.The differences in classification and distribution resulted in the sequence diversity.
基金Supported by Program of National Science and Technology Infra-structure (2005DKA21002-09)~~
文摘[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.from the Pearl River Basin in Guangdong and Guangxi Provinces.[Method] Genetic diversity of 64 local varieties of Morus atropurpurea Roxb.was analyzed by ISSR molecular marker technique.The genetic relationship among these local varieties was researched by UPGMA method based on the genetic similarity coefficient.[Result] 128 bands were amplified from the total DNA of 64 local varieties using 13 ISSR primers,of which 109 bands accounting for 85.15% were polymorphic.It meant that there was rich genetic diversity among the local varieties tested.The genetic similarity coefficients among 64 local varieties were relatively high with a range of 0.500 0-0.929 7.In addition,64 local varieties were divided into two categories and the second could be further divided into 10 subcategories.It was suggested that the genetic relationship of 64 local varieties of Morus atropurpurea Roxb.based on ISSR marker analysis has a certain correlation with geographical distribution.[Conclusion] ISSR marker technology was suitable for evaluating genetic relationship and genetic diversity of local varieties of Morus atropurpurea Roxb.in Pearl River Basin,and could provide scientific basis for DNA fingerprinting and identification of varieties of Morus atropurpurea Roxb.
基金The project supported by Research Development Unit,Faculty of Medicine Brawijaya University
文摘OBJECTIVE To investigate the effect of methanolic extract of Scurrula atropurpurea on systolic blood pressure and serum angiotensinⅡ concentration in deoxycorticosterone acetate-salt(DOCA-salt)hypertensive rats.METHODS Twenty-five male Wistar rats were divided into normal control group and four hypertensive groups by DOCA-salt for 6 weeks.Further,three hypertensive groups were orally administered with methanolic extract of Scurrula atropurpurea at a doses of 50,100 and 200mg·kg-1,respectively for 6 weeks and one hypertensive group without extract as positive control.At the end of research systolic blood pressure were measured by tail cuff methods.Serum angiotensinⅡ were measured by ELISA.RESULTS The systolic blood pressure increased significantly in the positive control hypertensive group compared to the normal control group(P<0.05).Administration of methanolic extract of Scurrula atropurpurea 50,100 and 200mg·kg-1 significantly decreased systolic blood pressure,respectively compared to the positive control hypertensive group(P<0.05).There was no different angiotensinⅡ consentration between positive control hypertensive group and normal group,while methanolic extract of Scurrula atropurpurea 50mg·kg-1 caused decreased angiotensinⅡ conscentration significantly compared to positive control hypertensive group(P<0.05).CONCLUSION Methanolic extract of Scurrula atropurpurea produced antihypertensive effect and decreasing angiotensinⅡ concentration at 50mg·kg-1 in hypertensive rat.Beside this,the concentration of angiotensinⅡ couldn′t increase in DOCA-salt hypertensive rats.
基金This work was supported by the 973 Program of Ministry of Science and Technology of China(2011CB915503).
文摘Smilacina atropurpurea(family Convallariaceae)is a perennial herb used medicinally for the trea tment of lung ailments,rheumatism,menstrual disorders,and cuts and bruises,by the local people of its growing areas in southwest China.The tender aerial part has also been used as a wild vegetable by Lisu,Naxi and Tibetan people.Chemical analysis of the rhizomes of this plant has allowed us to isolate two new minor steroidal tetraglycosides,specifically atropurosides H(1) and I(2),by repeated column chromatography over silica gel and reversed phase silica.The structures were determined to be(25R,S)-12β-hydroxy-spirost-5-ene-3β-yl-O-β-D-glucopyranosy1-(1→2)-[β-D-xylopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside(1)and(25S)-5α-spirost-17α-hydroxy-3β-yl-O-β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopy-ranosyl-(1→4)-β-D-galacto pyranoside(2),respectively,on the basis of detailed spectroscopic analysis including ID and 2D NM R techniques.
文摘【目的】针对桑树形态倍性鉴定及品种间鉴别问题，研究广东桑不同倍性及品种之间孢粉学上的异同，为桑树多倍体育种提供科学依据。【方法】利用扫描电镜对广东桑10个不同倍性品种花粉的形态、大小、萌发孔、外壁纹饰等孢粉学特性进行观察，并运用图片分析结合数理统计的方法对其花粉形态进行研究。【结果】供试广东桑不同品种的花粉微观形态相近，均为小型花粉，近球形，花粉粒极轴长16.98～24.46μm，赤道轴长15.37～23.86μm，极轴长与赤道轴长的比值为1.01～1.12；供试不同品种花粉粒萌发孔1～3个，圆形，直径为2.11～3.51μm，部分花粉粒萌发孔外壁内陷形成漏斗形，按照额尔特曼 G 的 NPC（萌发器官的数目、位置及特征）分类系统属N3P3C4类型；不同倍性间广东桑花粉的赤道轴长、极轴长和萌发孔大小存在显著性差异（P ＜0.05），均表现为四倍体＞二倍体，但花粉粒的极轴/赤轴比值差异不显著（P＞0.05）；广东桑花粉外壁为分布不均的刺状突起－脑条纹复合纹饰，不同品种间花粉的表面纹饰有明显差别，根据外壁纹饰可将供试10个品种花粉分为细刺颗粒状突起－细条纹纹饰、细刺颗粒状突起－粗条纹纹饰、粗刺颗粒状突起－细条纹纹饰和粗刺颗粒状突起－粗条纹纹饰4种类型，花粉外壁纹饰体现了不同品种的遗传多样性和遗传分化；不同倍性间广东桑花粉表面的刺状突起密度及大小差异不显著（P＞0.05）；供试四倍体广东桑品种花粉存在少数败育花粉粒，表现为干瘪而不充实，外形异常。【结论】供试广东桑不同倍性及品种间花粉形态特征具有差异，不同倍性间花粉粒和萌发孔大小存在差异，不同品种间花粉的表面纹饰存在明显差别，初步认为花粉的亚显微结构特征可作为广东桑倍性鉴定及品种间鉴别的重要标准之一。
