Algae-lysing bacteria A1 has good control effect on the moss through its extracellular secretion. In order to further study its control mechanism, the sin- gle-factor tests of temperature and pH value, the activated c...Algae-lysing bacteria A1 has good control effect on the moss through its extracellular secretion. In order to further study its control mechanism, the sin- gle-factor tests of temperature and pH value, the activated carbon adsorption test, the organic solvent extraction test and the crude extract of active substance test were carried out to explore the characteristics of active substances. The results showed that the algae-lysing active ingredient of A1 strain had strong thermal stabili- ty, which still had good algae-lysing effect after being treated at 121 ℃ ; when the pH values of fermentation liquid were adjusted to 2.0 and 4.0 respectively, the active substances lost their activities, but their control ability against moss would be enhanced under the alkaline conditions ; the active substances could not be ad- sorbed by activated carbon; the algae-lysing active substances showed strong hydrophilie ability when they were extracted by ethyl acetate, petroleum ether and chloroform. Therefore, it could be speculated that the active substances belonged to carbohydrates. The crude extract results of algae-lysing active substances showed that the active substances secreted by A1 strain were composed by a variety of algae-lysing active substances.展开更多
Water blooms have become a worldwide environmental problem. Recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. In this study, one strain of algicidal bacte...Water blooms have become a worldwide environmental problem. Recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. In this study, one strain of algicidal bacterium B5 was isolated from activated sludge. On the basis of analysis of its physiological characteristics and 16S rDNA gene sequence, it was identified as Bacillusfusiformis. Its algaelysing characteristics on Microcystis aeruginosa, Chlorella and Scenedesmus were tested. The results showed that： （1） the algicidal bacterium B5 is a Gram-negative bacterium. The 16S rDNA nucleotide sequence homology of strain B5 with 2 strains of B. fusiformis reached 99.86%, so B5 was identified as B. fusiformis; （2） the algal-lysing effects of the algicidal bacterium B5 on M. aeruginosa, Chlorella and Scenedesrnus were pronounced. The initial bacterial and algal cell densities strongly influence the removal rates of chlorophyll-a. The greater the initial bacterial cell density, the faster the degradation of chlorophyll-a. The greater the initial algal cell density, the slower the degradation of chlorophyll-a. When the bacterial cell density was 3.6 × 10^7 cells/ml, nearly 90% of chlorophyll-a was removed. When the chlorophyll-a concentration was less than 550 μg/L, about 70% was removed; （3） the strain B5 lysed algae by secreting metabolites and these metabolites could bear heat treatment.展开更多
This study aimed to evaluate the antibacterial activity of flavonoids extracted from two Libyan brown algae namely Cystoseira compressa and Padina pavonica using microwave-assisted extraction method against pathogenic...This study aimed to evaluate the antibacterial activity of flavonoids extracted from two Libyan brown algae namely Cystoseira compressa and Padina pavonica using microwave-assisted extraction method against pathogenic bacteria isolated from meat, meat products, milk and dairy products (Staphylococcus aureus subsp. aureus (5 isolates), Bacillus cereus (3 isolates), Bacillus pumilus (1 isolate), Salmonella enterica subsp. enteric (4 isolates) and Enterohaemor-rhagic Escherichia coli O157 (EHEC O157) (4 isolates)). All of these isolates were muti-drug resistant with high MAR index. The results showed that C. compressa extract exhibited better and stronger antibacterial activities against the seventeen tested isolates with inhibition zones diameter ranged from 14 - 22 mm compared to P. pavonica extract which showed positive effect against 9 isolates with low inhibition zone ranged from 11 - 16.5 mm. Flavonoids extracted from C. compressa also displayed the best spectrum of bactericidal effect with a ratio MBC/MIC ≤ 4 obtained on all susceptible tested bacterial strains. Flavonoids and proanthocyanidins significantly contributed to the antibacterial properties. The mode of action of these active extracts is under investigation.展开更多
Alga-lysing bacteria have been paid much attention to in recent years. In this study, the alga-lysing strain P05 which was isolated from an immobilizing biosystem was immobilized by coke and elastic filler, forming tw...Alga-lysing bacteria have been paid much attention to in recent years. In this study, the alga-lysing strain P05 which was isolated from an immobilizing biosystem was immobilized by coke and elastic filler, forming two biological reactors. The removal efficiencies of algae, NH4^＋-N and organic matter using the two reactors were studied. The results showed that strain P05 was an ideal algal-lysing bacteria strain because it was easy to be immobilized by coke and elastic filler which are of cheap, low biodegradability and the simple immobilization procedure. After 7 d filming, the biological film could be formed and the reactors were used to treat the eutrophic water. These two reactors were of stability and high effect with low cost and easy operation. The optimal hydraulic retention time （HRT） of each reactor was 4 h. The algae removal rates were 80.38% and 82.1% （in term of Chl-α） of coke reactor and filler reactor, respectively. And that of NH4^＋-N were 52.3% and 52.7%. The removal rates of CODMn were 39.03% and 39.64%. The strain P05 was identified as Bacillus sp. by PCR amplification of the 16S rRNA gene, BLAST analysis, and comparison with sequences in the GenBank nucleotide database.展开更多
Background： Micro-algae could inhibit the complete rumen BH of dietary 18-carbon unsaturated fatty acid （UFAs）. This study aimed to examine dose and time responses of algae supplementation on rumen fermentation, bi...Background： Micro-algae could inhibit the complete rumen BH of dietary 18-carbon unsaturated fatty acid （UFAs）. This study aimed to examine dose and time responses of algae supplementation on rumen fermentation, biohydrogenation and Butyrivibrio group bacteria in goats. Methods： Six goats were used in a repeated 3 x 3 Latin square design, and offered a fixed diet. Algae were infused through rumen cannule with 0 （Control）, 6.1 （L-AIg）, or 18.3 g （H-AIg） per day. Rumen contents were sampled on d 0, 3, 7, 14 and 20. Results： H-AIg reduced total volatile fatty acid concentration and acetate molar proportion （P 〈 0.05）, and increased propionate molar proportion （P 〈 0.05）, whereas L-AIg had no effect on rumen fermentation. Changes in proportions of acetate and propionate in H-AIg were obvious from d 7 onwards and reached the largest differences with the control on d 14. Algae induced a dose-dependent decrease in 18：0 and increased trons-18：1 in the ruminal content （P 〈 0.05）. H-AIg increased the concentrations of t9, t] 1-18：2 and tl 1, cl 5-18：2 （P 〈 0.05）. L-AIg only seemed to induce a transient change in 18-carbon isomers, while H-AIg induced a rapid elevation, already obvious on d 3, concentrations of these fatty acid rose in some cases again on d 20. Algae had no effect on the abundances of Butyrivibfio spp. and Butyrivibrio proteoclosdcus （P 〉 0.10）, while H-AIg reduced the total bacteria abundance （P 〈 0.05）. However, this was induced by a significant difference between control and H-AIg on d 14 （-4.43 %）. Afterwards, both treatments did not differ as increased variation in the H-AIg repetitions, with in some cases a return of the bacterial abundance to the basal level （d 0）. Conclusions： Changes in rumen fermentation and 18-carbon UFAs metabolism in response to algae were related to the supplementation level, but there was no evidence of shift in ruminal biohydrogenation pathways towards t1 0-18：1 L-AIg mainly induced a transient effect on rumen biohydrogenation of 18-carbon UFAs, while H-AIg showed an acute inhibition and these effects were not associated with the known hydrogenating bacteria.展开更多
In the paper, under the framework of exploring the interaction between algae and bacteria, an algae-bacteria ecological model was established to analyze the interaction mechanism and growth coexistence mode between al...In the paper, under the framework of exploring the interaction between algae and bacteria, an algae-bacteria ecological model was established to analyze the interaction mechanism and growth coexistence mode between algicidal bacteria and algae. Firstly, mathematical work mainly provided some threshold conditions to ensure the occurrence of transcritical bifurcation and saddle-node bifurcation, which could provide certain theoretical support for selecting key ecological environmental factors and numerical simulations. Secondly, the numerical simulation work dynamically displayed the evolution process of the bifurcation dynamic behavior of the model (2.1) and the growth coexistence mode of algae and algicidal bacteria. Finally, it was worth summarizing that intrinsic growth rate and combined capture effort of algae population had a strong influence on the dynamic behavior of the model (2.1). Furthermore, it must also be noted that transcritical bifurcation and saddle-node bifurcation were the inherent driving forces behind the formation of steady-state growth coexistence mode between algicidal bacteria and algae. In summary, it was hoped that the results of this study would contribute to accelerating the study of the interaction mechanism between algicidal bacteria and algae.展开更多
We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses.Twelve strains of bacteria were obtained from the surfaces of the following four sp...We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses.Twelve strains of bacteria were obtained from the surfaces of the following four species of algae:Gracilaria textorii,Ulva pertusa,Laminaria japonica,and Polysiphonia urceolata.The isolated strains of bacteria can be divided into two groups:Halomonas and Vibrio,in physiology,biochemical characteristics and 16S rDNA sequence analyses.The phylogenetic tree constructed based on 16S rDNA sequences of the isolates shows four obvious clusters,Halomonas venusta,Vibrio tasmaniensis,Vibrio lentus,and Vibrio splendidus.Isolates from the surface of P.urceolata are more abundant and diverse,of which strains P9 and P28 have a 16S rDNA sequence very similar(97.5%-99.8%) to that of V.splendidus.On the contrary,the isolates from the surfaces of G.textorii,U.pertusa and L.japonica are quite simple and distribute on different branches of the phylogenetic tree.In overall,the results of this study indicate that the genetic relationships among the isolates are quite close and display a certain level of host species specificity,and alga-associated bacteria species are algal species specific.展开更多
Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using t...Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance （NMR） and Fourier Transform infrared （FT-IR） spectroscopy. Algae-lysing activity was observed using microscopy. Results The algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin （C20H25N30）, which showed strong lytic activity with algal strains M. aeruginoso TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration （ICs0） of prodigiosin with the algal strains was 4.8 （±0.4）×10^-2 μg/mL, 8.9 （±1.1）×10^-2μg/mL, and 1.7 （±0.1）×10^-1 μg/mL in 24 h, respectively. Conclusion The bacterium LTH-2 and its pigment related to damage of cell membranes. The bacterium for regulating blooms of harmful M. aeruginosa. had strong Microcystis-lysing activity probably LTH-2 and its red pigment are potentially useful展开更多
An anode was constructed using a novel technique and subsequently tested in a bio-battery. The anode comprised of a composite electrode coated with immobilized bacteria. The immobilized bacteria used in this study wer...An anode was constructed using a novel technique and subsequently tested in a bio-battery. The anode comprised of a composite electrode coated with immobilized bacteria. The immobilized bacteria used in this study were Escherichia coli K-12. The composite electrode contained three layers: a 304 L stainless steel mesh base, an electro-polymerized layer of pyrrole, and an electro-polymerized layer of methylene blue. The bacteria were immobilized utilizing a technique incorporating a carbon nanoparticle and Teflon<sup>TM</sup> emulsion. The composite electrode combined with immobilized bacteria was examined whilst incorporated into the anodic chamber of a bio-battery. Different tests were conducted, including Electrochemical Impedance Spectroscopy. Results from these tests were compared with data obtained from alternate configurations and values from the open literature. The maximum power density generated by the composite electrode with immobilized bacteria whilst incorporated into the anodic chamber of a bio-battery was 378 mW/m<sup>2</sup>. Results demonstrate this composite anode configuration with immobilized bacteria produced approximately 69% more power density and 53% more current density than alternate electrode configurations with bacteria suspended in solution. Also, it was found that a significant portion of the bio-battery’s resistance to charge transfer occurred at the surface of the anode and this resistance was lowered by 51% through bacteria immobilization.展开更多
基金Supported by Scientific and Technological Research Projects in Suihua University(KQ1002004)~~
文摘Algae-lysing bacteria A1 has good control effect on the moss through its extracellular secretion. In order to further study its control mechanism, the sin- gle-factor tests of temperature and pH value, the activated carbon adsorption test, the organic solvent extraction test and the crude extract of active substance test were carried out to explore the characteristics of active substances. The results showed that the algae-lysing active ingredient of A1 strain had strong thermal stabili- ty, which still had good algae-lysing effect after being treated at 121 ℃ ; when the pH values of fermentation liquid were adjusted to 2.0 and 4.0 respectively, the active substances lost their activities, but their control ability against moss would be enhanced under the alkaline conditions ; the active substances could not be ad- sorbed by activated carbon; the algae-lysing active substances showed strong hydrophilie ability when they were extracted by ethyl acetate, petroleum ether and chloroform. Therefore, it could be speculated that the active substances belonged to carbohydrates. The crude extract results of algae-lysing active substances showed that the active substances secreted by A1 strain were composed by a variety of algae-lysing active substances.
基金Project supported by the Special Funds for Doctor's Station of University(No.20060246024)Young Fund of Fudan University,and the Shanghai Tongji Gao Tingyao Environmental Science and Technology Developmem Fundation
文摘Water blooms have become a worldwide environmental problem. Recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. In this study, one strain of algicidal bacterium B5 was isolated from activated sludge. On the basis of analysis of its physiological characteristics and 16S rDNA gene sequence, it was identified as Bacillusfusiformis. Its algaelysing characteristics on Microcystis aeruginosa, Chlorella and Scenedesmus were tested. The results showed that： （1） the algicidal bacterium B5 is a Gram-negative bacterium. The 16S rDNA nucleotide sequence homology of strain B5 with 2 strains of B. fusiformis reached 99.86%, so B5 was identified as B. fusiformis; （2） the algal-lysing effects of the algicidal bacterium B5 on M. aeruginosa, Chlorella and Scenedesrnus were pronounced. The initial bacterial and algal cell densities strongly influence the removal rates of chlorophyll-a. The greater the initial bacterial cell density, the faster the degradation of chlorophyll-a. The greater the initial algal cell density, the slower the degradation of chlorophyll-a. When the bacterial cell density was 3.6 × 10^7 cells/ml, nearly 90% of chlorophyll-a was removed. When the chlorophyll-a concentration was less than 550 μg/L, about 70% was removed; （3） the strain B5 lysed algae by secreting metabolites and these metabolites could bear heat treatment.
文摘This study aimed to evaluate the antibacterial activity of flavonoids extracted from two Libyan brown algae namely Cystoseira compressa and Padina pavonica using microwave-assisted extraction method against pathogenic bacteria isolated from meat, meat products, milk and dairy products (Staphylococcus aureus subsp. aureus (5 isolates), Bacillus cereus (3 isolates), Bacillus pumilus (1 isolate), Salmonella enterica subsp. enteric (4 isolates) and Enterohaemor-rhagic Escherichia coli O157 (EHEC O157) (4 isolates)). All of these isolates were muti-drug resistant with high MAR index. The results showed that C. compressa extract exhibited better and stronger antibacterial activities against the seventeen tested isolates with inhibition zones diameter ranged from 14 - 22 mm compared to P. pavonica extract which showed positive effect against 9 isolates with low inhibition zone ranged from 11 - 16.5 mm. Flavonoids extracted from C. compressa also displayed the best spectrum of bactericidal effect with a ratio MBC/MIC ≤ 4 obtained on all susceptible tested bacterial strains. Flavonoids and proanthocyanidins significantly contributed to the antibacterial properties. The mode of action of these active extracts is under investigation.
基金Project supported by the Special Funds for Ph. D Research Station of University (No. 20020422045)the Science Foundation of Shandong Province (No. Z2003B01)the Environmental Protection Bureau of Shandong Province, China.
文摘Alga-lysing bacteria have been paid much attention to in recent years. In this study, the alga-lysing strain P05 which was isolated from an immobilizing biosystem was immobilized by coke and elastic filler, forming two biological reactors. The removal efficiencies of algae, NH4^＋-N and organic matter using the two reactors were studied. The results showed that strain P05 was an ideal algal-lysing bacteria strain because it was easy to be immobilized by coke and elastic filler which are of cheap, low biodegradability and the simple immobilization procedure. After 7 d filming, the biological film could be formed and the reactors were used to treat the eutrophic water. These two reactors were of stability and high effect with low cost and easy operation. The optimal hydraulic retention time （HRT） of each reactor was 4 h. The algae removal rates were 80.38% and 82.1% （in term of Chl-α） of coke reactor and filler reactor, respectively. And that of NH4^＋-N were 52.3% and 52.7%. The removal rates of CODMn were 39.03% and 39.64%. The strain P05 was identified as Bacillus sp. by PCR amplification of the 16S rRNA gene, BLAST analysis, and comparison with sequences in the GenBank nucleotide database.
基金funded by the Natural Science Foundation of Jiangsu Province (China)the Research Foundation-Flanders (Belgium)the Special Research Fund of the Ghent University (Belgium)
文摘Background： Micro-algae could inhibit the complete rumen BH of dietary 18-carbon unsaturated fatty acid （UFAs）. This study aimed to examine dose and time responses of algae supplementation on rumen fermentation, biohydrogenation and Butyrivibrio group bacteria in goats. Methods： Six goats were used in a repeated 3 x 3 Latin square design, and offered a fixed diet. Algae were infused through rumen cannule with 0 （Control）, 6.1 （L-AIg）, or 18.3 g （H-AIg） per day. Rumen contents were sampled on d 0, 3, 7, 14 and 20. Results： H-AIg reduced total volatile fatty acid concentration and acetate molar proportion （P 〈 0.05）, and increased propionate molar proportion （P 〈 0.05）, whereas L-AIg had no effect on rumen fermentation. Changes in proportions of acetate and propionate in H-AIg were obvious from d 7 onwards and reached the largest differences with the control on d 14. Algae induced a dose-dependent decrease in 18：0 and increased trons-18：1 in the ruminal content （P 〈 0.05）. H-AIg increased the concentrations of t9, t] 1-18：2 and tl 1, cl 5-18：2 （P 〈 0.05）. L-AIg only seemed to induce a transient change in 18-carbon isomers, while H-AIg induced a rapid elevation, already obvious on d 3, concentrations of these fatty acid rose in some cases again on d 20. Algae had no effect on the abundances of Butyrivibfio spp. and Butyrivibrio proteoclosdcus （P 〉 0.10）, while H-AIg reduced the total bacteria abundance （P 〈 0.05）. However, this was induced by a significant difference between control and H-AIg on d 14 （-4.43 %）. Afterwards, both treatments did not differ as increased variation in the H-AIg repetitions, with in some cases a return of the bacterial abundance to the basal level （d 0）. Conclusions： Changes in rumen fermentation and 18-carbon UFAs metabolism in response to algae were related to the supplementation level, but there was no evidence of shift in ruminal biohydrogenation pathways towards t1 0-18：1 L-AIg mainly induced a transient effect on rumen biohydrogenation of 18-carbon UFAs, while H-AIg showed an acute inhibition and these effects were not associated with the known hydrogenating bacteria.
文摘In the paper, under the framework of exploring the interaction between algae and bacteria, an algae-bacteria ecological model was established to analyze the interaction mechanism and growth coexistence mode between algicidal bacteria and algae. Firstly, mathematical work mainly provided some threshold conditions to ensure the occurrence of transcritical bifurcation and saddle-node bifurcation, which could provide certain theoretical support for selecting key ecological environmental factors and numerical simulations. Secondly, the numerical simulation work dynamically displayed the evolution process of the bifurcation dynamic behavior of the model (2.1) and the growth coexistence mode of algae and algicidal bacteria. Finally, it was worth summarizing that intrinsic growth rate and combined capture effort of algae population had a strong influence on the dynamic behavior of the model (2.1). Furthermore, it must also be noted that transcritical bifurcation and saddle-node bifurcation were the inherent driving forces behind the formation of steady-state growth coexistence mode between algicidal bacteria and algae. In summary, it was hoped that the results of this study would contribute to accelerating the study of the interaction mechanism between algicidal bacteria and algae.
基金Supported by the National Natural Science Foundation of China (No 40376048)the National Basic Research Program of China (973 Program) (No 2006CB400604)
文摘We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses.Twelve strains of bacteria were obtained from the surfaces of the following four species of algae:Gracilaria textorii,Ulva pertusa,Laminaria japonica,and Polysiphonia urceolata.The isolated strains of bacteria can be divided into two groups:Halomonas and Vibrio,in physiology,biochemical characteristics and 16S rDNA sequence analyses.The phylogenetic tree constructed based on 16S rDNA sequences of the isolates shows four obvious clusters,Halomonas venusta,Vibrio tasmaniensis,Vibrio lentus,and Vibrio splendidus.Isolates from the surface of P.urceolata are more abundant and diverse,of which strains P9 and P28 have a 16S rDNA sequence very similar(97.5%-99.8%) to that of V.splendidus.On the contrary,the isolates from the surfaces of G.textorii,U.pertusa and L.japonica are quite simple and distribute on different branches of the phylogenetic tree.In overall,the results of this study indicate that the genetic relationships among the isolates are quite close and display a certain level of host species specificity,and alga-associated bacteria species are algal species specific.
基金supported by the National Science and Technology Major Project (2012ZX07101-005)the National Natural Science Foundation of China (30972440)Jiangsu Province Postgraduate Innovation Project (CX10B-087Z)
文摘Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance （NMR） and Fourier Transform infrared （FT-IR） spectroscopy. Algae-lysing activity was observed using microscopy. Results The algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin （C20H25N30）, which showed strong lytic activity with algal strains M. aeruginoso TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration （ICs0） of prodigiosin with the algal strains was 4.8 （±0.4）×10^-2 μg/mL, 8.9 （±1.1）×10^-2μg/mL, and 1.7 （±0.1）×10^-1 μg/mL in 24 h, respectively. Conclusion The bacterium LTH-2 and its pigment related to damage of cell membranes. The bacterium for regulating blooms of harmful M. aeruginosa. had strong Microcystis-lysing activity probably LTH-2 and its red pigment are potentially useful
文摘An anode was constructed using a novel technique and subsequently tested in a bio-battery. The anode comprised of a composite electrode coated with immobilized bacteria. The immobilized bacteria used in this study were Escherichia coli K-12. The composite electrode contained three layers: a 304 L stainless steel mesh base, an electro-polymerized layer of pyrrole, and an electro-polymerized layer of methylene blue. The bacteria were immobilized utilizing a technique incorporating a carbon nanoparticle and Teflon<sup>TM</sup> emulsion. The composite electrode combined with immobilized bacteria was examined whilst incorporated into the anodic chamber of a bio-battery. Different tests were conducted, including Electrochemical Impedance Spectroscopy. Results from these tests were compared with data obtained from alternate configurations and values from the open literature. The maximum power density generated by the composite electrode with immobilized bacteria whilst incorporated into the anodic chamber of a bio-battery was 378 mW/m<sup>2</sup>. Results demonstrate this composite anode configuration with immobilized bacteria produced approximately 69% more power density and 53% more current density than alternate electrode configurations with bacteria suspended in solution. Also, it was found that a significant portion of the bio-battery’s resistance to charge transfer occurred at the surface of the anode and this resistance was lowered by 51% through bacteria immobilization.
文摘城镇污水中的碳、氮、磷等污染物主要是采用微生物法进行去除,需通过曝气、补碳等方法保证出水达标。藻菌共生体系是利用微藻产氧气驱动硝化过程去除氨氮,减少曝气成本,并且促进藻菌形成絮体,提高沉降性能。实验结果表明,当光暗比为18 h:6 h时,藻菌系统对氨氮的去除率最高达到82.57%,此时叶绿素a浓度和叶绿素荧光参强度Fv/Fm最高,分别为112.54μg/L和0.53。此外,扫描电子显微镜(Scanning Electron Microscope,SEM)结果显示,相较于单独污泥系统,藻菌体系中形成紧密的絮体结构,提高了藻菌沉降性能。
