OBJECTIVES:To investigate the influence of catgut implantation at acupoints on leptin resistance(LR) and insulin resistance(IR) in the simple obesity rat.METHODS:Rats were made obese with high-fat diets,after which su...OBJECTIVES:To investigate the influence of catgut implantation at acupoints on leptin resistance(LR) and insulin resistance(IR) in the simple obesity rat.METHODS:Rats were made obese with high-fat diets,after which surgical catgut was implanted at Zusanli(ST 36) and Neiting(ST 44) acupoints once a week for 4 weeks(implantation group).Rats from the implantation group were compared with normal rats and unoperated obese rats(control group).Gene expression of the leptin receptor(OB-R) was evaluated using RT-PCR and northern blot.Serum and hypothalamus leptin and insulin(INS) levels were determined by radioimmunoassay.Body weight,Lee's index,body fat,serum and hypothalamus leptin and INS levels,and hypothalamic OB-R gene expression were determined before and after treatment.RESULTS:Body weights,Lee's index,body fat,and serum leptin and INS levels were significantly higher in obese than in normal rats.Hypothalamic leptin and INS levels and OB-R gene expression were significantly lower in obese rats.Catgut implantation at acupoint promoted weight loss and decreased serum leptin and INS levels.Hypothalamic leptin and INS levels and OB-R gene expression increased significantly.CONCLUSIONS:Catgut implantation at acupoint adjusts central and peripheral leptin and promotes hypothalamic OB-R gene expression.This may be an important method for regulation of LR,IR and abnormal endocrinology and metabolism.展开更多
Amygdaloid nucleus(AMYG)could converge and integrate the signals from electroacupuncture(EA)at Neiguan(P 6)and acute myocardial ischemia(AMI)in rabbits.And EA could regulatethe changes in the discharge of single units...Amygdaloid nucleus(AMYG)could converge and integrate the signals from electroacupuncture(EA)at Neiguan(P 6)and acute myocardial ischemia(AMI)in rabbits.And EA could regulatethe changes in the discharge of single units in AMYG caused by AMI,that is,EA inhibited theincreased frequency,and reversed the decreased frequency.This suggested that AMYG is an im-portant link in correlation between the heart and the acupoint Neiguan,providing an experimen-tal basis for the Theory of Channel-Viscera Correlation.展开更多
基金Supported by Science Research Projects of Colleges and Universities of Liaoning Provincial Committee of Education (Project number:05L121)
文摘OBJECTIVES:To investigate the influence of catgut implantation at acupoints on leptin resistance(LR) and insulin resistance(IR) in the simple obesity rat.METHODS:Rats were made obese with high-fat diets,after which surgical catgut was implanted at Zusanli(ST 36) and Neiting(ST 44) acupoints once a week for 4 weeks(implantation group).Rats from the implantation group were compared with normal rats and unoperated obese rats(control group).Gene expression of the leptin receptor(OB-R) was evaluated using RT-PCR and northern blot.Serum and hypothalamus leptin and insulin(INS) levels were determined by radioimmunoassay.Body weight,Lee's index,body fat,serum and hypothalamus leptin and INS levels,and hypothalamic OB-R gene expression were determined before and after treatment.RESULTS:Body weights,Lee's index,body fat,and serum leptin and INS levels were significantly higher in obese than in normal rats.Hypothalamic leptin and INS levels and OB-R gene expression were significantly lower in obese rats.Catgut implantation at acupoint promoted weight loss and decreased serum leptin and INS levels.Hypothalamic leptin and INS levels and OB-R gene expression increased significantly.CONCLUSIONS:Catgut implantation at acupoint adjusts central and peripheral leptin and promotes hypothalamic OB-R gene expression.This may be an important method for regulation of LR,IR and abnormal endocrinology and metabolism.
基金The project was supported by National Natural Science Foundation of China.
文摘Amygdaloid nucleus(AMYG)could converge and integrate the signals from electroacupuncture(EA)at Neiguan(P 6)and acute myocardial ischemia(AMI)in rabbits.And EA could regulatethe changes in the discharge of single units in AMYG caused by AMI,that is,EA inhibited theincreased frequency,and reversed the decreased frequency.This suggested that AMYG is an im-portant link in correlation between the heart and the acupoint Neiguan,providing an experimen-tal basis for the Theory of Channel-Viscera Correlation.
