Mount Tai Scenic Area has achieved certain results in the construction of accessible tourism facilities,realizing the exploration of digital and intelligent transformation in traditional mountain scenic areas.In this ...Mount Tai Scenic Area has achieved certain results in the construction of accessible tourism facilities,realizing the exploration of digital and intelligent transformation in traditional mountain scenic areas.In this study,using questionnaire surveys and in-depth interviews,the development of accessible tourism in Mount Tai Scenic Area was investigated and analyzed.Some countermeasures were put forward,including strengthening information guidance services,enhancing awareness and skills in accessibility services,improving feedback mechanisms for continuous refinement,and strengthening collaborations in the fields of the disabled and accessibility development,aiming to further promote humanistic care and enhance service satisfaction at the scenic area.展开更多
AIM: To select the optimal antisense accessible sites of survivin, a highly expressed gene in tumor tissues, in order to explore a novel approach to improve biological therapy of gastric cancer. METHODS: The 20 mer ra...AIM: To select the optimal antisense accessible sites of survivin, a highly expressed gene in tumor tissues, in order to explore a novel approach to improve biological therapy of gastric cancer. METHODS: The 20 mer random oligonucleotide library was synthesized, hybridized with in vitro transcribed total survivin cRNA, then digested by RNase H. After primer extension and autoradiography, the antisense accessible sites (AAS) of survivin were selected. Then RNADraw software was used to analyze and choose the AAS with obvious stem-loop structures, according to which the complementary antisense oligonucleotides (AS-ODNs) were synthesized and transferred into survivin highly- expressing gastric cancer cell line MKN-45. Survivin expression was detected by RT-PCR and Western Blotting. Cellular growth activities were assayed by tetrazolium bromide (MTT) colorimetry. Cellular ultrastructure was observed by electronic microscopy, while apoptosis was detected by annexin V-FITC and propidium iodide staining flow cytometry. RESULTS: Thirteen AAS of survivin were selected In vitro. Four AAS with stem-loop structures were chosen, locating at 207-226 bp, 187-206 bp, 126-145 bp and 44-63 bp of survivin cDNA respectively. When compared with non-tranfection controls, their corresponding AS-ODNs (AS-ODN1, AS-ODN2, AS-ODN3 and AS-ODN4) could reduce Survivin mRNA levels in MKN-45 cells by 54.3±±1.1% (t= 6.12, P<0.01), 86.1±±1.0% (t= 5.27, P<0.01), 32.2±±1.3% (t= 7.34, P<0.01) and 56.2±±0.9% (t = 6.45, P<0.01) respectively, while survivin protein levels were decreased by 42.2±±2.5% (t = 6.26, P<0.01), 75.4±±3.1% (t= 7.11, P<0.01), 28.3±±2.0% (t= 6.04, P<0.01) and 45.8±±1.2% (t = 6.38,P<0.01) respectively. After transfection with 600 nmol/L AS-ODN1-AS-ODN4for 24 h, cell growth was inhibited by 28.12±±1.54% (t= 7.62, P<0.01), 38.42±±3.12% (t= 7.75, P<0.01), 21.46±±2.63% (t= 5.94, P<0.01) and 32.12±1.77% (t = 6.17, P<0.01) respectively. Partial cancer cells presented the characteristic morphological changes of apoptosis, with apoptotic rates being 19.31±1.16% (t= 7.16,P<0.01), 29.24±1.94% (t = 8.15,P<0.01), 11.87±0.68% (t = 6.68, P<0.01) and 21.68±2.14% (t = 7.53, P<0.01) respectively. CONCLUSION: The MS of survivin could be effectively selected in vitro by random oligonucleotide library/RNase H cleavage method combined with computer software analysis, this has important reference values for further studying survivin-targeted therapy strategies for gastric cancer.展开更多
The regression model for octanol/water partition coefficients ( K ow ), is founded with only two molecular descriptors available through quantum chemical calculations: solvation free energy (Δ G S ), and so...The regression model for octanol/water partition coefficients ( K ow ), is founded with only two molecular descriptors available through quantum chemical calculations: solvation free energy (Δ G S ), and solvent accessible surface area (SASA). For the properties of 47 organic compounds from 17 types, the model gives a correction coefficient (adjusted for degrees of freedom) of 0 959 and a standard error of 0 277 log unit. It is a suitable way to predict the partition properties that are related to solute solvent interactions in the water phase.展开更多
The optimal antisense accessible sites （AAS） of uroplakin Ⅱ （UP Ⅱ ） mRNA, a specific gene expressed in bladder urothelium, were selected in order to provide a novel method for targeted therapy of transitional ce...The optimal antisense accessible sites （AAS） of uroplakin Ⅱ （UP Ⅱ ） mRNA, a specific gene expressed in bladder urothelium, were selected in order to provide a novel method for targeted therapy of transitional cell carcinoma （TCC） of bladder. The 20 mer random oligonucleotide library was synthesized, hybridized with in vitro transcripted total UPⅡ cRNA, then digested by RNase H. After primer extension and autoradiography, the AAS of UPⅡ were selected. The RNADraw soft- ware was used to analyze and choose the AAS with obvious stem-loop structures, according to which the complementary antisense oligonucleotides （AS-ODN） were synthesized. With the AS-ODN0 designed only by RNADraw as a control, the AS-ODNs were transferred into UP Ⅱ highly-expressing cell line RT4. The cellular expression of UPⅡ mRNA was detected by RT-PCR and Western blot. Twelve AAS of UPⅡ mRNA were selected in vitro. Four AAS with stem-loop structures were chosen, locating at 558-577, 552-571, 217-236 and 97-116 bp of UP Ⅱ mRNA respectively. After transfection with the corresponding AS-ODN （AS-ODN1, AS-ODN2, AS-ODN3 and AS-ODN4） for 18 h, the UPⅡ mRNA levels in RT4cells were reduced by 29,3%, 82.7%, 71.3% and 70.9%, while UP Ⅱ protein levels were decreased by 20.2%, 78.5%, 65.2% and 64.4% respectively, which were significantly higher than those of AS-ODN0 （14.3%, 12.1% respectively） （P〈0.01）. The AAS of UPⅡ mRNA was effectively selected in vitro by random oligonucletide library/RNase H cleavage method in combination with computer software analysis, which had important reference values for further studying biological functions of UP Ⅱ gene and targeted therapeutic strategy for TCC of bladder.展开更多
The topics of inclusion and disability are currently discussed in modern society.In particular,the concept of accessibility draws a wide range of attention legislation,architecture and tourism.Accessible tourism is a ...The topics of inclusion and disability are currently discussed in modern society.In particular,the concept of accessibility draws a wide range of attention legislation,architecture and tourism.Accessible tourism is a quality perspective of tourism,and it provides a panorama of change and evolution.The paper,which focuses on the city of Rome,analyzes the advantages and criticisms of these"forgotten"and underestimated market niches.Information,transports,space distribution,and safety are involved in the development of the accessible destination.The paper opens a reflection about the opportunity and the perspective of improving a quality and accessible tourism in the Eternal City.展开更多
The aim of this paper is to show a theoretical approach to the evolution of concepts perceiving disability, taking into account the medical, social, and geographical models, as the basis for the development of princip...The aim of this paper is to show a theoretical approach to the evolution of concepts perceiving disability, taking into account the medical, social, and geographical models, as the basis for the development of principles concerning the organisation of accessible tourism for people with disabilities （PWD）. The main research objective was to identify the current attitudes of future, potential employees in the tourism （tourism and recreation students at the time of the study） towards accessible tourism. The study was based on surveys performed in May 2013 at the Adam Mickiewicz University in Poznafi （UAM, Poland） and the State University in Irkutsk （HFY, Russia）, a total sample of 216 people. The main section of the survey contained four questions regarding issues such as： optimal ways to organise tourism products for people with a disability; attitudes towards spending leisure time together with people with a disability; and specific requirements concerning the introduction of various types of improvements in tourism products aimed at people with a disability. In both cases, the results revealed that future tourism employees hold attitudes which are prevailingly open and positive towards the needs of tourists with disabilities. However, the hypothesis that the main factor influencing a reluctance to enter into contact with PwD is a lack of experience in this area, resulting in insufficient knowledge of what conditions the behaviour of PwD was also confirmed. This is a highly significant conclusion which should consider if mandatory educational programmes in the field of tourism and recreation studies are to be improved.展开更多
Fe-NX/C electrocatalysts have aroused extensive interest in accelerating sluggish oxygen reduction reaction (ORR) kinetics as potential alternatives to platinum catalysts in rechargeable Zn-air batteries (ZABs).Howeve...Fe-NX/C electrocatalysts have aroused extensive interest in accelerating sluggish oxygen reduction reaction (ORR) kinetics as potential alternatives to platinum catalysts in rechargeable Zn-air batteries (ZABs).However,the low density and poor accessibility of Fe-NXsites have severely restricted the electrocatalytic performance of Fe-NX/C.Herein,Fe,N co-doped ordered mesoporous carbon fiber bundles are prepared through a ligand-assisted strategy with nitrogen-rich 1,10-phenanthroline as space isolation agent.1,10-Phenanthroline reveals a six-membered heterocyclic structure containing abundant nitrogen species to tightly coordinate with Fe ions,which is conducive to achieving high-density Fe-NXsites.Meanwhile,the adoption of SBA-15 as hard-templates enables the catalysts with highly ordered channels and large specific surface areas,improving the accessibility of Fe-NXsites.The optimal catalyst (PDA-Fe-900) demonstrates a positive half-wave potential of 0.84 V (vs.RHE) in alkaline solution,outperforming the commercial Pt/C (0.83 V).In addition,PDA-Fe-900 delivers comparable ORR performance to commercial Pt/C in acidic electrolyte.Impressively,when PDA-Fe-900 is employed as an air cathode,it achieves large power densities of 163.0 m W/cm^(2) in liquid-state ZAB and 116.6 m W/cm^(2) in the flexible solid-state ZAB.This work provides an efficient ligand-assisted pathway for fabricating catalysts with dense and accessible FeNXsites as high-performance ORR electrocatalysts for ZABs.展开更多
Background:A comprehensive landscape of chromatin states for multiple mammalian tissues is essential for elucidating the molecular mechanism underlying regulatory variants on complex traits.However,the genome-wide chr...Background:A comprehensive landscape of chromatin states for multiple mammalian tissues is essential for elucidating the molecular mechanism underlying regulatory variants on complex traits.However,the genome-wide chromatin accessibility has been only reported in limited tissue types in pigs.Results:Here we report a genome-wide landscape of chromatin accessibility of 20 tissues in two female pigs at ages of 6 months using ATAC-seq,and identified 557,273 merged peaks,which greatly expanded the pig regulatory ele-ment repository.We revealed tissue-specific regulatory elements which were associated with tissue-relevant biologi-cal functions.We identified both positive and negative significant correlations between the regulatory elements and gene transcripts,which showed distinct distributions in terms of their strength and distances from corresponding genes.We investigated the presence of transposable elements(TEs)in open chromatin regions across all tissues,these included identifications of porcine endogenous retroviruses(PERVs)exhibiting high accessibility in liver and homology of porcine specific virus sequences to universally accessible transposable elements.Furthermore,we prior-itized a potential causal variant for polyunsaturated fatty acid in the muscle.Conclusions:Our data provides a novel multi-tissues accessible chromatin landscape that serve as an important resource for interpreting regulatory sequences in tissue-specific and conserved biological functions,as well as regula-tory variants of loci associated with complex traits in pigs.展开更多
To solve the problems of current IP multicast which includes poor inter-domain many-to-many group support, security vulnerabilities and dependency to specific multicast infrastructure, a mobile accessible closed multi...To solve the problems of current IP multicast which includes poor inter-domain many-to-many group support, security vulnerabilities and dependency to specific multicast infrastructure, a mobile accessible closed multi-part group （MACMPG） communication protocol in IPv6 network is proposed. By extending the single source multicast protocol, the communication channel for multi-part group communication across domains is established. Based on lPv6 CGA, the secure closed group communication scheme is designed. The access to the multicast traffic only confined to the authorized senders and receivers and only trusted routers are allowed to be the branch points of MACMPG tree. By tunneling mechanism, the MACMPG traffic can be transmitted across non-MACMPG routing area, and the mobile nodes can join the group remotely and roam freely between domains, which eliminates the dependency on specific IP multicast routing.展开更多
Although serious efforts are made internationally and nationally, it is a slow process to make our physical environment accessible. In the actual design process, architects play a major role. But what kinds of knowled...Although serious efforts are made internationally and nationally, it is a slow process to make our physical environment accessible. In the actual design process, architects play a major role. But what kinds of knowledge, including research-based knowledge, do practicing architects make use of when designing accessible environments？ The answer to the question is crucially important since it affects how knowledge is distributed and how accessibility can be ensured. In order to get first-hand knowledge about the design process and the sources from which they gain knowledge, 11 qualitative interviews were conducted with architects with experience of designing for accessibility. The analysis draws on two theoretical distinctions. The first is research-based knowledge versus knowledge used by architects. The second is context-independent knowledge versus context-dependent knowledge. The practitioners found their primary support in context-dependent knowledge, whereas context-independent knowledge was criticized as being too prescriptive. Further, they tended to ask for assistance from the researcher in person rather than reading research publications. The findings challenge research in two ways--first, to produce context-dependent knowledge to structure the first step of the design process, second, to develop new ways to ensure a knowledge flow between research and practice.展开更多
AS the raging novel coronavirus disease(COVID-19)enveloped all of China,a myriad of cultural institutions across the country have been temporarily closed to reduce the flow of people,including museums,public libraries...AS the raging novel coronavirus disease(COVID-19)enveloped all of China,a myriad of cultural institutions across the country have been temporarily closed to reduce the flow of people,including museums,public libraries,cultural centers,extra-curricular training courses,and even cinemas.However,a diverse range of dynamic cultural activities,enabled and powered by technological advancements and Internet-based technological platforms,are vying to provide the public with rich online cultural experiences and content.展开更多
A result revealed by the Department of Statistics, 29,000 elderly people have registered for Visually Impaired Card in 2014, which was 51.2% of all visually impaired people. With annual growth rate of 1.56%, this numb...A result revealed by the Department of Statistics, 29,000 elderly people have registered for Visually Impaired Card in 2014, which was 51.2% of all visually impaired people. With annual growth rate of 1.56%, this number increased yearly by 1,100 people. According to WHO, 285 million people worldwide were estimated to be visually impaired, which is 4.24% of overall population in 2012. From age distribution point of view, most visually impaired people, accounted for 2.76% of overall population, were above age 50. In average, there are 7 visually impaired people in every 100 people above 50 years old. In Taiwan, 549,000 people over age 50 are estimated to be visually impaired. Therefore we expect there is a large amount of visually impaired people. The main purpose of this research is to collect adaptability information on the cognitive model of senior visually impaired people on their work status, social participation status, and leisure activities via questionnaire survey. Furthermore, descriptive video service is used to as an accessible long-term care application for visually impaired senior users. To solve disability circumstances and improve home care quality through visually impaired APP POe （proof-of-concept）. The result of this research will serve as an important basis for other researches in related study field, a reference for practice application. As a result in encouraging profit-seeking enterprises design a user oriented products. And even be an opening for mobile accessibility services benchmark on technology social care for disability and senior users.展开更多
The main characteristics of potential smart cities in the future, smart citizens, smart energy, smart buildings, smart mobility, smart technology, smart healthcare, smart infrastructure, smart governance and education...The main characteristics of potential smart cities in the future, smart citizens, smart energy, smart buildings, smart mobility, smart technology, smart healthcare, smart infrastructure, smart governance and education, and finally smart security are the aspects of smart cities. In the smart city, smart accessible infrastructure creates an inclusive environment for people with disabilities, in which they can integrate into the community and enjoy their civil, cultural, political, social and economic rights and entitlements. The aim of this study is to integrate disabled people into society so that they can actively participate in society and lead to a normal life. An integrated approach is needed to ensure that people with disabilities are not left alone. A combination of mainstreaming and targeted action is required for all interventions. People with disabilities have the same basic needs as everyone else: health protection and treatment, basic services, housing and income. Targeted intervention measures need to complement disability mainstreaming by addressing specific needs identified by general responses, including such interventions need not add materially to the overall cost, especially if the requirements of the maximum number of users are considered in the initial planning. This paper recommends how we can use an appropriate design checklist as a guide for our initial architectural concept and smart city planning to facilities reasonably accessible for people with disabilities in private and public buildings, making our cities smarter and more inclusive and helping to improve the quality of life for all people, especially people with disabilities.展开更多
A description of the Systems Dynamics paradigm is given and the reduced Qualitative System Dynamics (QSD) form explained. A simple example is given to illustrate the diagram construction. The principles of states (lev...A description of the Systems Dynamics paradigm is given and the reduced Qualitative System Dynamics (QSD) form explained. A simple example is given to illustrate the diagram construction. The principles of states (levels), rates and feedback loops are outlined. The QSD method is used to address the problem of accessibility by using human control of automation as an example, and applying the QSD method to evaluate the effects of the researcher and user in the de- sign of an accessible artefact. This simple automation model illustrates what can be found out from such a picture, in this indicating how the feedback from users has an influence on the time to deliver such designs.展开更多
Recently,Li Qun,Vice Minister of the Ministry of Culture and Tourism and Director of National Cultural Heritage Administration(NCHA),said that China has a total of 6,565 museums with more than 90%museums accessible to...Recently,Li Qun,Vice Minister of the Ministry of Culture and Tourism and Director of National Cultural Heritage Administration(NCHA),said that China has a total of 6,565 museums with more than 90%museums accessible to the public for free.展开更多
The success of the Beijing Winter Olympics and Paralympics epitomized the vision of a community with a shared future for humankind.Various robots and artificial intelligence technologies were used in various games and...The success of the Beijing Winter Olympics and Paralympics epitomized the vision of a community with a shared future for humankind.Various robots and artificial intelligence technologies were used in various games and events,such as assisting in parades,logistics,and security inspections.The integration of unmanned robotic systems into torch relays was one of the highlights of the Winter Olympics.展开更多
The nitrogen-coordinated metal single-atom catalysts(M−N−C SACs)with an ultra-high metal loading synthetized by direct high-temperature pyrolysis have been widely reported.However,most of metal single atoms in these c...The nitrogen-coordinated metal single-atom catalysts(M−N−C SACs)with an ultra-high metal loading synthetized by direct high-temperature pyrolysis have been widely reported.However,most of metal single atoms in these catalysts were buried in the carbon matrix,resulting in a low metal utilization and inaccessibility for adsorption of reactants during the catalytic process.Herein,we reported a facile synthesis based on the hard-soft acid-base(HSAB)theory to fabricate Co single-atom catalysts with highly exposed metal atoms ligated to the external pyridinic-N sites of a nitrogen-doped carbon support.Benefiting from the highly accessible Co active sites,the prepared Co−N−C SAC exhibited a superior oxygen reduction reactivity comparable to that of the commercial Pt/C catalyst,showing a high turnover frequency(TOF)of 0.93 e^(−)·s^(-1)·site^(-1)at 0.85 V vs.RHE,far exceeding those of some representative SACs with a ultra-high metal content.This work provides a rational strategy to design and prepare M−N−C single-atom catalysts featured with high site-accessibility and site-density.展开更多
基金Sponsored by Tai’an Science and Technology Innovation Development Project(Policy Guidance Category)(2024ZC042)Research Project of Inner Mongolia Autonomous Region Center for the Theoretical System of Socialism with Chinese Characteristics(2024ZZB018).
文摘Mount Tai Scenic Area has achieved certain results in the construction of accessible tourism facilities,realizing the exploration of digital and intelligent transformation in traditional mountain scenic areas.In this study,using questionnaire surveys and in-depth interviews,the development of accessible tourism in Mount Tai Scenic Area was investigated and analyzed.Some countermeasures were put forward,including strengthening information guidance services,enhancing awareness and skills in accessibility services,improving feedback mechanisms for continuous refinement,and strengthening collaborations in the fields of the disabled and accessibility development,aiming to further promote humanistic care and enhance service satisfaction at the scenic area.
基金Supported by National Natural Science Foundation of China, No.30200284Science Foundation of Huazhong University of Science and Technology
文摘AIM: To select the optimal antisense accessible sites of survivin, a highly expressed gene in tumor tissues, in order to explore a novel approach to improve biological therapy of gastric cancer. METHODS: The 20 mer random oligonucleotide library was synthesized, hybridized with in vitro transcribed total survivin cRNA, then digested by RNase H. After primer extension and autoradiography, the antisense accessible sites (AAS) of survivin were selected. Then RNADraw software was used to analyze and choose the AAS with obvious stem-loop structures, according to which the complementary antisense oligonucleotides (AS-ODNs) were synthesized and transferred into survivin highly- expressing gastric cancer cell line MKN-45. Survivin expression was detected by RT-PCR and Western Blotting. Cellular growth activities were assayed by tetrazolium bromide (MTT) colorimetry. Cellular ultrastructure was observed by electronic microscopy, while apoptosis was detected by annexin V-FITC and propidium iodide staining flow cytometry. RESULTS: Thirteen AAS of survivin were selected In vitro. Four AAS with stem-loop structures were chosen, locating at 207-226 bp, 187-206 bp, 126-145 bp and 44-63 bp of survivin cDNA respectively. When compared with non-tranfection controls, their corresponding AS-ODNs (AS-ODN1, AS-ODN2, AS-ODN3 and AS-ODN4) could reduce Survivin mRNA levels in MKN-45 cells by 54.3±±1.1% (t= 6.12, P<0.01), 86.1±±1.0% (t= 5.27, P<0.01), 32.2±±1.3% (t= 7.34, P<0.01) and 56.2±±0.9% (t = 6.45, P<0.01) respectively, while survivin protein levels were decreased by 42.2±±2.5% (t = 6.26, P<0.01), 75.4±±3.1% (t= 7.11, P<0.01), 28.3±±2.0% (t= 6.04, P<0.01) and 45.8±±1.2% (t = 6.38,P<0.01) respectively. After transfection with 600 nmol/L AS-ODN1-AS-ODN4for 24 h, cell growth was inhibited by 28.12±±1.54% (t= 7.62, P<0.01), 38.42±±3.12% (t= 7.75, P<0.01), 21.46±±2.63% (t= 5.94, P<0.01) and 32.12±1.77% (t = 6.17, P<0.01) respectively. Partial cancer cells presented the characteristic morphological changes of apoptosis, with apoptotic rates being 19.31±1.16% (t= 7.16,P<0.01), 29.24±1.94% (t = 8.15,P<0.01), 11.87±0.68% (t = 6.68, P<0.01) and 21.68±2.14% (t = 7.53, P<0.01) respectively. CONCLUSION: The MS of survivin could be effectively selected in vitro by random oligonucleotide library/RNase H cleavage method combined with computer software analysis, this has important reference values for further studying survivin-targeted therapy strategies for gastric cancer.
基金TheNationalNaturalScienceFoundationofChina (No .2 9837180 )andtheResearchFoundationfortheDoctoralProgramofHigherEducationofChi
文摘The regression model for octanol/water partition coefficients ( K ow ), is founded with only two molecular descriptors available through quantum chemical calculations: solvation free energy (Δ G S ), and solvent accessible surface area (SASA). For the properties of 47 organic compounds from 17 types, the model gives a correction coefficient (adjusted for degrees of freedom) of 0 959 and a standard error of 0 277 log unit. It is a suitable way to predict the partition properties that are related to solute solvent interactions in the water phase.
基金supported by grants from the National Natural Sciences Foundation of China (No. 30200284, No. 30600278, No. 30772359)Program for New Century Excellent Talents in University (NCET-06-0641)Scientific Research Foundation for the Returned Overseas Chinese Scholars (2008889)
文摘The optimal antisense accessible sites （AAS） of uroplakin Ⅱ （UP Ⅱ ） mRNA, a specific gene expressed in bladder urothelium, were selected in order to provide a novel method for targeted therapy of transitional cell carcinoma （TCC） of bladder. The 20 mer random oligonucleotide library was synthesized, hybridized with in vitro transcripted total UPⅡ cRNA, then digested by RNase H. After primer extension and autoradiography, the AAS of UPⅡ were selected. The RNADraw soft- ware was used to analyze and choose the AAS with obvious stem-loop structures, according to which the complementary antisense oligonucleotides （AS-ODN） were synthesized. With the AS-ODN0 designed only by RNADraw as a control, the AS-ODNs were transferred into UP Ⅱ highly-expressing cell line RT4. The cellular expression of UPⅡ mRNA was detected by RT-PCR and Western blot. Twelve AAS of UPⅡ mRNA were selected in vitro. Four AAS with stem-loop structures were chosen, locating at 558-577, 552-571, 217-236 and 97-116 bp of UP Ⅱ mRNA respectively. After transfection with the corresponding AS-ODN （AS-ODN1, AS-ODN2, AS-ODN3 and AS-ODN4） for 18 h, the UPⅡ mRNA levels in RT4cells were reduced by 29,3%, 82.7%, 71.3% and 70.9%, while UP Ⅱ protein levels were decreased by 20.2%, 78.5%, 65.2% and 64.4% respectively, which were significantly higher than those of AS-ODN0 （14.3%, 12.1% respectively） （P〈0.01）. The AAS of UPⅡ mRNA was effectively selected in vitro by random oligonucletide library/RNase H cleavage method in combination with computer software analysis, which had important reference values for further studying biological functions of UP Ⅱ gene and targeted therapeutic strategy for TCC of bladder.
文摘The topics of inclusion and disability are currently discussed in modern society.In particular,the concept of accessibility draws a wide range of attention legislation,architecture and tourism.Accessible tourism is a quality perspective of tourism,and it provides a panorama of change and evolution.The paper,which focuses on the city of Rome,analyzes the advantages and criticisms of these"forgotten"and underestimated market niches.Information,transports,space distribution,and safety are involved in the development of the accessible destination.The paper opens a reflection about the opportunity and the perspective of improving a quality and accessible tourism in the Eternal City.
文摘The aim of this paper is to show a theoretical approach to the evolution of concepts perceiving disability, taking into account the medical, social, and geographical models, as the basis for the development of principles concerning the organisation of accessible tourism for people with disabilities （PWD）. The main research objective was to identify the current attitudes of future, potential employees in the tourism （tourism and recreation students at the time of the study） towards accessible tourism. The study was based on surveys performed in May 2013 at the Adam Mickiewicz University in Poznafi （UAM, Poland） and the State University in Irkutsk （HFY, Russia）, a total sample of 216 people. The main section of the survey contained four questions regarding issues such as： optimal ways to organise tourism products for people with a disability; attitudes towards spending leisure time together with people with a disability; and specific requirements concerning the introduction of various types of improvements in tourism products aimed at people with a disability. In both cases, the results revealed that future tourism employees hold attitudes which are prevailingly open and positive towards the needs of tourists with disabilities. However, the hypothesis that the main factor influencing a reluctance to enter into contact with PwD is a lack of experience in this area, resulting in insufficient knowledge of what conditions the behaviour of PwD was also confirmed. This is a highly significant conclusion which should consider if mandatory educational programmes in the field of tourism and recreation studies are to be improved.
基金supported by the National Natural Science Foundation of China(No.U1804255)the Key Research&Development and Promotion Projects in Henan Province(Nos.222102520038 and 212102210651)。
文摘Fe-NX/C electrocatalysts have aroused extensive interest in accelerating sluggish oxygen reduction reaction (ORR) kinetics as potential alternatives to platinum catalysts in rechargeable Zn-air batteries (ZABs).However,the low density and poor accessibility of Fe-NXsites have severely restricted the electrocatalytic performance of Fe-NX/C.Herein,Fe,N co-doped ordered mesoporous carbon fiber bundles are prepared through a ligand-assisted strategy with nitrogen-rich 1,10-phenanthroline as space isolation agent.1,10-Phenanthroline reveals a six-membered heterocyclic structure containing abundant nitrogen species to tightly coordinate with Fe ions,which is conducive to achieving high-density Fe-NXsites.Meanwhile,the adoption of SBA-15 as hard-templates enables the catalysts with highly ordered channels and large specific surface areas,improving the accessibility of Fe-NXsites.The optimal catalyst (PDA-Fe-900) demonstrates a positive half-wave potential of 0.84 V (vs.RHE) in alkaline solution,outperforming the commercial Pt/C (0.83 V).In addition,PDA-Fe-900 delivers comparable ORR performance to commercial Pt/C in acidic electrolyte.Impressively,when PDA-Fe-900 is employed as an air cathode,it achieves large power densities of 163.0 m W/cm^(2) in liquid-state ZAB and 116.6 m W/cm^(2) in the flexible solid-state ZAB.This work provides an efficient ligand-assisted pathway for fabricating catalysts with dense and accessible FeNXsites as high-performance ORR electrocatalysts for ZABs.
基金supported by National Key Research and Development Program of China(2021YFF1000601).
文摘Background:A comprehensive landscape of chromatin states for multiple mammalian tissues is essential for elucidating the molecular mechanism underlying regulatory variants on complex traits.However,the genome-wide chromatin accessibility has been only reported in limited tissue types in pigs.Results:Here we report a genome-wide landscape of chromatin accessibility of 20 tissues in two female pigs at ages of 6 months using ATAC-seq,and identified 557,273 merged peaks,which greatly expanded the pig regulatory ele-ment repository.We revealed tissue-specific regulatory elements which were associated with tissue-relevant biologi-cal functions.We identified both positive and negative significant correlations between the regulatory elements and gene transcripts,which showed distinct distributions in terms of their strength and distances from corresponding genes.We investigated the presence of transposable elements(TEs)in open chromatin regions across all tissues,these included identifications of porcine endogenous retroviruses(PERVs)exhibiting high accessibility in liver and homology of porcine specific virus sequences to universally accessible transposable elements.Furthermore,we prior-itized a potential causal variant for polyunsaturated fatty acid in the muscle.Conclusions:Our data provides a novel multi-tissues accessible chromatin landscape that serve as an important resource for interpreting regulatory sequences in tissue-specific and conserved biological functions,as well as regula-tory variants of loci associated with complex traits in pigs.
基金the National Natural Science Foundation of China (60503012)the National High Technology Research and Develop-ment Program of China (2007AA01Z405)
文摘To solve the problems of current IP multicast which includes poor inter-domain many-to-many group support, security vulnerabilities and dependency to specific multicast infrastructure, a mobile accessible closed multi-part group （MACMPG） communication protocol in IPv6 network is proposed. By extending the single source multicast protocol, the communication channel for multi-part group communication across domains is established. Based on lPv6 CGA, the secure closed group communication scheme is designed. The access to the multicast traffic only confined to the authorized senders and receivers and only trusted routers are allowed to be the branch points of MACMPG tree. By tunneling mechanism, the MACMPG traffic can be transmitted across non-MACMPG routing area, and the mobile nodes can join the group remotely and roam freely between domains, which eliminates the dependency on specific IP multicast routing.
文摘Although serious efforts are made internationally and nationally, it is a slow process to make our physical environment accessible. In the actual design process, architects play a major role. But what kinds of knowledge, including research-based knowledge, do practicing architects make use of when designing accessible environments？ The answer to the question is crucially important since it affects how knowledge is distributed and how accessibility can be ensured. In order to get first-hand knowledge about the design process and the sources from which they gain knowledge, 11 qualitative interviews were conducted with architects with experience of designing for accessibility. The analysis draws on two theoretical distinctions. The first is research-based knowledge versus knowledge used by architects. The second is context-independent knowledge versus context-dependent knowledge. The practitioners found their primary support in context-dependent knowledge, whereas context-independent knowledge was criticized as being too prescriptive. Further, they tended to ask for assistance from the researcher in person rather than reading research publications. The findings challenge research in two ways--first, to produce context-dependent knowledge to structure the first step of the design process, second, to develop new ways to ensure a knowledge flow between research and practice.
文摘AS the raging novel coronavirus disease(COVID-19)enveloped all of China,a myriad of cultural institutions across the country have been temporarily closed to reduce the flow of people,including museums,public libraries,cultural centers,extra-curricular training courses,and even cinemas.However,a diverse range of dynamic cultural activities,enabled and powered by technological advancements and Internet-based technological platforms,are vying to provide the public with rich online cultural experiences and content.
文摘A result revealed by the Department of Statistics, 29,000 elderly people have registered for Visually Impaired Card in 2014, which was 51.2% of all visually impaired people. With annual growth rate of 1.56%, this number increased yearly by 1,100 people. According to WHO, 285 million people worldwide were estimated to be visually impaired, which is 4.24% of overall population in 2012. From age distribution point of view, most visually impaired people, accounted for 2.76% of overall population, were above age 50. In average, there are 7 visually impaired people in every 100 people above 50 years old. In Taiwan, 549,000 people over age 50 are estimated to be visually impaired. Therefore we expect there is a large amount of visually impaired people. The main purpose of this research is to collect adaptability information on the cognitive model of senior visually impaired people on their work status, social participation status, and leisure activities via questionnaire survey. Furthermore, descriptive video service is used to as an accessible long-term care application for visually impaired senior users. To solve disability circumstances and improve home care quality through visually impaired APP POe （proof-of-concept）. The result of this research will serve as an important basis for other researches in related study field, a reference for practice application. As a result in encouraging profit-seeking enterprises design a user oriented products. And even be an opening for mobile accessibility services benchmark on technology social care for disability and senior users.
文摘The main characteristics of potential smart cities in the future, smart citizens, smart energy, smart buildings, smart mobility, smart technology, smart healthcare, smart infrastructure, smart governance and education, and finally smart security are the aspects of smart cities. In the smart city, smart accessible infrastructure creates an inclusive environment for people with disabilities, in which they can integrate into the community and enjoy their civil, cultural, political, social and economic rights and entitlements. The aim of this study is to integrate disabled people into society so that they can actively participate in society and lead to a normal life. An integrated approach is needed to ensure that people with disabilities are not left alone. A combination of mainstreaming and targeted action is required for all interventions. People with disabilities have the same basic needs as everyone else: health protection and treatment, basic services, housing and income. Targeted intervention measures need to complement disability mainstreaming by addressing specific needs identified by general responses, including such interventions need not add materially to the overall cost, especially if the requirements of the maximum number of users are considered in the initial planning. This paper recommends how we can use an appropriate design checklist as a guide for our initial architectural concept and smart city planning to facilities reasonably accessible for people with disabilities in private and public buildings, making our cities smarter and more inclusive and helping to improve the quality of life for all people, especially people with disabilities.
文摘A description of the Systems Dynamics paradigm is given and the reduced Qualitative System Dynamics (QSD) form explained. A simple example is given to illustrate the diagram construction. The principles of states (levels), rates and feedback loops are outlined. The QSD method is used to address the problem of accessibility by using human control of automation as an example, and applying the QSD method to evaluate the effects of the researcher and user in the de- sign of an accessible artefact. This simple automation model illustrates what can be found out from such a picture, in this indicating how the feedback from users has an influence on the time to deliver such designs.
文摘Recently,Li Qun,Vice Minister of the Ministry of Culture and Tourism and Director of National Cultural Heritage Administration(NCHA),said that China has a total of 6,565 museums with more than 90%museums accessible to the public for free.
基金This study was supported by the National Natural Science Foundation of China(U22A2067,62103406,61821005,61925307,and 62333007)。
文摘The success of the Beijing Winter Olympics and Paralympics epitomized the vision of a community with a shared future for humankind.Various robots and artificial intelligence technologies were used in various games and events,such as assisting in parades,logistics,and security inspections.The integration of unmanned robotic systems into torch relays was one of the highlights of the Winter Olympics.
基金supported by Shanxi Province Science Foundation for Youths(202203021212300)Taiyuan University of Science and Technology Scientific Research Initial Funding(20212064)Outstanding Doctoral Award Fund in Shanxi Province(20222060).
文摘The nitrogen-coordinated metal single-atom catalysts(M−N−C SACs)with an ultra-high metal loading synthetized by direct high-temperature pyrolysis have been widely reported.However,most of metal single atoms in these catalysts were buried in the carbon matrix,resulting in a low metal utilization and inaccessibility for adsorption of reactants during the catalytic process.Herein,we reported a facile synthesis based on the hard-soft acid-base(HSAB)theory to fabricate Co single-atom catalysts with highly exposed metal atoms ligated to the external pyridinic-N sites of a nitrogen-doped carbon support.Benefiting from the highly accessible Co active sites,the prepared Co−N−C SAC exhibited a superior oxygen reduction reactivity comparable to that of the commercial Pt/C catalyst,showing a high turnover frequency(TOF)of 0.93 e^(−)·s^(-1)·site^(-1)at 0.85 V vs.RHE,far exceeding those of some representative SACs with a ultra-high metal content.This work provides a rational strategy to design and prepare M−N−C single-atom catalysts featured with high site-accessibility and site-density.
