Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of th...Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining展开更多
The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that...The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that the maize hybrid impurity is mainly composed of three parts:mixed with the seeds from parents or other source, contaminated with the seeds resulting from unknown pollination and variable in genetic background. It was found that the varietal purity is higher than the zymogram purity and they showed a linear relationship. The regression coefficient (r) between varietal purity and zymogram purity is 0.898 2. There is an average of 3.5% variable in the field. However,using a known rate of the mixed seeds, their regression coefficient is 0.999 9. It was also found that the examinational mix rate is 0.33% higher than the known mix rate. The seedling rate of the single cross hybrids is 85.71%,but that of hybrids mixed with their parental lines is only 77%. Therefore,it is reasonable to claim that zymogram purity is lower than the varietal purity.展开更多
[Objectives]To explore the clinical effect of Modified Gegen Qinlian Decoction combined with western medicine on children with rotavirus enteritis with damp-heat syndrome and its influence on myocardial enzymes.[Metho...[Objectives]To explore the clinical effect of Modified Gegen Qinlian Decoction combined with western medicine on children with rotavirus enteritis with damp-heat syndrome and its influence on myocardial enzymes.[Methods]One hundred and eighty children with rotavirus enteritis with damp-heat syndrome were randomly divided into control group and observation group,with 90 cases in each group.The control group was given western medicine symptomatic treatment,and the observation group was given Modified Gegen Qinlian Decoction treatment on the basis of the control group.Both groups were treated for 5 d.The clinical efficacy,symptom disappearance time,myocardial zymogram indicator level and TCM syndrome scores were compared between the two groups.[Results]After 5 d of treatment,the total effective rate of the observation group was 94.44%,which was significantly higher than that of the control group(84.44%)(P<0.05).The time of diarrhea,fever,vomiting,dehydration and fecal RV turning negative in the observation group were shorter than those in the control group,and the differences were statistically significant(P<0.05).After 5 d of treatment,the serum levels of creatine kinase(CK),creatine kinase isoenzyme(CK-MB),aspartate aminotransferase(AST),lactate dehydrogenase(LDH)and α-hydroxybutyrate dehydrogenase(α-HBDH)in the two groups were lower than those before treatment.The levels of the above five myocardial enzymes in the observation group were lower than those in the control group,and the differences were statistically significant(P<0.05).After 5 d of treatment,the scores of defecation frequency,abdominal distention,thirst,dysphoria,fatigue and anorexia in the two groups were lower than those before treatment.The scores of the above five TCM syndromes in the observation group were lower than those in the control group,and the differences were statistically significant(P<0.05).[Conclusions]Modified Gegen Qinlian Decoction combined with western medicine can quickly relieve the symptoms and signs of children with rotavirus enteritis with damp-heat syndrome,shorten the course of disease,and reduce myocardial injury.展开更多
文摘Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining
文摘The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that the maize hybrid impurity is mainly composed of three parts:mixed with the seeds from parents or other source, contaminated with the seeds resulting from unknown pollination and variable in genetic background. It was found that the varietal purity is higher than the zymogram purity and they showed a linear relationship. The regression coefficient (r) between varietal purity and zymogram purity is 0.898 2. There is an average of 3.5% variable in the field. However,using a known rate of the mixed seeds, their regression coefficient is 0.999 9. It was also found that the examinational mix rate is 0.33% higher than the known mix rate. The seedling rate of the single cross hybrids is 85.71%,but that of hybrids mixed with their parental lines is only 77%. Therefore,it is reasonable to claim that zymogram purity is lower than the varietal purity.
文摘[Objectives]To explore the clinical effect of Modified Gegen Qinlian Decoction combined with western medicine on children with rotavirus enteritis with damp-heat syndrome and its influence on myocardial enzymes.[Methods]One hundred and eighty children with rotavirus enteritis with damp-heat syndrome were randomly divided into control group and observation group,with 90 cases in each group.The control group was given western medicine symptomatic treatment,and the observation group was given Modified Gegen Qinlian Decoction treatment on the basis of the control group.Both groups were treated for 5 d.The clinical efficacy,symptom disappearance time,myocardial zymogram indicator level and TCM syndrome scores were compared between the two groups.[Results]After 5 d of treatment,the total effective rate of the observation group was 94.44%,which was significantly higher than that of the control group(84.44%)(P<0.05).The time of diarrhea,fever,vomiting,dehydration and fecal RV turning negative in the observation group were shorter than those in the control group,and the differences were statistically significant(P<0.05).After 5 d of treatment,the serum levels of creatine kinase(CK),creatine kinase isoenzyme(CK-MB),aspartate aminotransferase(AST),lactate dehydrogenase(LDH)and α-hydroxybutyrate dehydrogenase(α-HBDH)in the two groups were lower than those before treatment.The levels of the above five myocardial enzymes in the observation group were lower than those in the control group,and the differences were statistically significant(P<0.05).After 5 d of treatment,the scores of defecation frequency,abdominal distention,thirst,dysphoria,fatigue and anorexia in the two groups were lower than those before treatment.The scores of the above five TCM syndromes in the observation group were lower than those in the control group,and the differences were statistically significant(P<0.05).[Conclusions]Modified Gegen Qinlian Decoction combined with western medicine can quickly relieve the symptoms and signs of children with rotavirus enteritis with damp-heat syndrome,shorten the course of disease,and reduce myocardial injury.
