Objective:It has been documented that ezrin/radixin/moesin(ERM)phosphorylation by the p38 mitogen-activated protein kinase(MAPK),Rho/ROCK,and protein kinase C(PKC)pathways leads to filamentous actin(F-actin)reorganiza...Objective:It has been documented that ezrin/radixin/moesin(ERM)phosphorylation by the p38 mitogen-activated protein kinase(MAPK),Rho/ROCK,and protein kinase C(PKC)pathways leads to filamentous actin(F-actin)reorganization and microvascular endothelial cell hyperpermeability.In this study,we investigated the effects of Xijiao Dihuang Decoction combined with Yinqiao Powder(XDY)on influenza virus(IV)-induced F-actin restructuring and ERM phosphorylation regulated by the Rho/Rho kinase 1(ROCK),p38 MAPK,and PKC signaling pathways in pulmonary microvascular endothelial cells(PMVECs).Methods:Serum containing XDY(XDY-CS;13.8 g/kg)was acquired using standard protocols for serum pharmacology.Primary PMVECs were obtained from male Wistar rats and cultured.After adsorption of IV A(multiplicity of infection,0.01)for 1 h,medium with 20%XDY-CS was added to the PMVECs.The distributions of F-actin and phosphorylated ERM were determined by confocal microscopy,and F-actin expression was measured by flow cytometry.The expression levels of ROCK1,phosphorylated myosin phosphatase target-subunit(p-MYPT),phosphorylated MAPK kinase,phosphorylated p38(p-p38),phosphorylated PKC(p-PKC),and phosphorylated ERM(p-ERM)were determined by western blotting.Results:F-actin reorganization in IV-infected PMVECs was reversed by XDY-CS treatment,which was accompanied by reduced p-ERM production.The p-ERM protein accumulated at plasma membrane of PMVECs infected with IV,which was also inhibited by XDY-CS treatment.展开更多
Yinqiao powder comes from the book"Wenbing Tiaobian"(Detailed Analysis of Epidemic Warm Diseases)written by Wu Jutong in the Qing Dynasty(1636-1912),in which it is mentioned that"pungent-cool diaphoreti...Yinqiao powder comes from the book"Wenbing Tiaobian"(Detailed Analysis of Epidemic Warm Diseases)written by Wu Jutong in the Qing Dynasty(1636-1912),in which it is mentioned that"pungent-cool diaphoretic agent Yinqiao power is used to treat patients with fever,not aversion to cold but thirsty"Yinqiao powder is often used to treat Wind-warm syndrome,warm-heat syndrome and miscellaneous diseases caused by some pathogenic factors in Wei-fen syndrome(Wei-fen syndrome is the syndrome name of the disease and is often used to indicate the initial stage of exogenous febrile disease,which is the syndrome of the body's guard surface.)and upper energizer.With the further research and application of Yinqiao powder,it is more and more widely used in clinic.This article summarizes the clinical application of Yinqiao powder through the analysis of the prescription meaning,compatible prescriptions,decoction methods and other aspects of Yinqiao powder in order to provide more evidence for clinical treatment of febrile disease.At the same time,through the prescription thinking of Yinqiao powder,we can learn Wu Jutong's treatment principle and academic thought of"Shangjiao(Shangjiao is one of the three and is the upper part of the Sanjiao.Generally refers to the chest above the diaphragm,including the heart,lungs and throat.)is like a feather,not lightly".Wu Jutong's flexible use of Chinese medicine and the thought of syndrome differentiation and treatment are also worthy of our study and reference.展开更多
Acute lung injury(ALI)is a respiratory disease characterized by inflammation.Yinqiao powder(YQP)is a traditional Chinese medicinal compound,extensively used for its heat-clearing and detoxifying effects.It is usually ...Acute lung injury(ALI)is a respiratory disease characterized by inflammation.Yinqiao powder(YQP)is a traditional Chinese medicinal compound,extensively used for its heat-clearing and detoxifying effects.It is usually used clinically to treat respiratory diseases,such as pneumonia.However,the exact mechanism by which YQP treats ALI remains unclear.Here,we aimed to explore the therapeutic effects and potential mechanisms of action of YQP in ALI.Activation of the cGAS-STING pathway was modeled by multiple stimuli in bone marrow-derived macrophages(BMDMs),THP-1,and peripheral blood mononuclear cells(PBMCs).Changes in the content of associated proteins were detected by Western blot,while the mRNA levels of type I interferon-related genes and proinflammatory cytokines were detected by qPCR.A DMXAA-induced agonist model was used to verify the effect of YQP on the cGAS-STING pathway in vivo,whereas LPS-induced ALI was used to explore the therapeutic effect of YQP.YQP suppressed the activation of the cGAS-STING pathway in vitro.YQP showed promising therapeutic effects on DMXAA-induced activation of the cGAS-STING signaling pathway as well as the LPS-induced ALI model in mice.YQP alleviated ALI by inhibiting cGAS-STING pathway activation;meanwhile,YQP may be a new therapeutic approach for cGAS-STING-driven inflammatory diseases.展开更多
基金This work was supported by funding from the National Natural Science Foundation of China(Grant Nos.81473520 and 81102697).
文摘Objective:It has been documented that ezrin/radixin/moesin(ERM)phosphorylation by the p38 mitogen-activated protein kinase(MAPK),Rho/ROCK,and protein kinase C(PKC)pathways leads to filamentous actin(F-actin)reorganization and microvascular endothelial cell hyperpermeability.In this study,we investigated the effects of Xijiao Dihuang Decoction combined with Yinqiao Powder(XDY)on influenza virus(IV)-induced F-actin restructuring and ERM phosphorylation regulated by the Rho/Rho kinase 1(ROCK),p38 MAPK,and PKC signaling pathways in pulmonary microvascular endothelial cells(PMVECs).Methods:Serum containing XDY(XDY-CS;13.8 g/kg)was acquired using standard protocols for serum pharmacology.Primary PMVECs were obtained from male Wistar rats and cultured.After adsorption of IV A(multiplicity of infection,0.01)for 1 h,medium with 20%XDY-CS was added to the PMVECs.The distributions of F-actin and phosphorylated ERM were determined by confocal microscopy,and F-actin expression was measured by flow cytometry.The expression levels of ROCK1,phosphorylated myosin phosphatase target-subunit(p-MYPT),phosphorylated MAPK kinase,phosphorylated p38(p-p38),phosphorylated PKC(p-PKC),and phosphorylated ERM(p-ERM)were determined by western blotting.Results:F-actin reorganization in IV-infected PMVECs was reversed by XDY-CS treatment,which was accompanied by reduced p-ERM production.The p-ERM protein accumulated at plasma membrane of PMVECs infected with IV,which was also inhibited by XDY-CS treatment.
文摘Yinqiao powder comes from the book"Wenbing Tiaobian"(Detailed Analysis of Epidemic Warm Diseases)written by Wu Jutong in the Qing Dynasty(1636-1912),in which it is mentioned that"pungent-cool diaphoretic agent Yinqiao power is used to treat patients with fever,not aversion to cold but thirsty"Yinqiao powder is often used to treat Wind-warm syndrome,warm-heat syndrome and miscellaneous diseases caused by some pathogenic factors in Wei-fen syndrome(Wei-fen syndrome is the syndrome name of the disease and is often used to indicate the initial stage of exogenous febrile disease,which is the syndrome of the body's guard surface.)and upper energizer.With the further research and application of Yinqiao powder,it is more and more widely used in clinic.This article summarizes the clinical application of Yinqiao powder through the analysis of the prescription meaning,compatible prescriptions,decoction methods and other aspects of Yinqiao powder in order to provide more evidence for clinical treatment of febrile disease.At the same time,through the prescription thinking of Yinqiao powder,we can learn Wu Jutong's treatment principle and academic thought of"Shangjiao(Shangjiao is one of the three and is the upper part of the Sanjiao.Generally refers to the chest above the diaphragm,including the heart,lungs and throat.)is like a feather,not lightly".Wu Jutong's flexible use of Chinese medicine and the thought of syndrome differentiation and treatment are also worthy of our study and reference.
基金funded by the project of Cultivating and Improving the Service Ability of Traditional Chinese Medicine(No.2021ZY038)National Natural Science Foundation of China(No.82474095)General Hospital 3+1 Innovative Talent Construction Project.
文摘Acute lung injury(ALI)is a respiratory disease characterized by inflammation.Yinqiao powder(YQP)is a traditional Chinese medicinal compound,extensively used for its heat-clearing and detoxifying effects.It is usually used clinically to treat respiratory diseases,such as pneumonia.However,the exact mechanism by which YQP treats ALI remains unclear.Here,we aimed to explore the therapeutic effects and potential mechanisms of action of YQP in ALI.Activation of the cGAS-STING pathway was modeled by multiple stimuli in bone marrow-derived macrophages(BMDMs),THP-1,and peripheral blood mononuclear cells(PBMCs).Changes in the content of associated proteins were detected by Western blot,while the mRNA levels of type I interferon-related genes and proinflammatory cytokines were detected by qPCR.A DMXAA-induced agonist model was used to verify the effect of YQP on the cGAS-STING pathway in vivo,whereas LPS-induced ALI was used to explore the therapeutic effect of YQP.YQP suppressed the activation of the cGAS-STING pathway in vitro.YQP showed promising therapeutic effects on DMXAA-induced activation of the cGAS-STING signaling pathway as well as the LPS-induced ALI model in mice.YQP alleviated ALI by inhibiting cGAS-STING pathway activation;meanwhile,YQP may be a new therapeutic approach for cGAS-STING-driven inflammatory diseases.
