摘要
目的:观察银翘散对复发性口腔溃疡(recurrent oral ulcer,ROU)大鼠的作用,并探讨其对IκB激酶β亚基(IkB kinaseβ,IKKβ)/核因子κB p65亚基(nuclear factor kappa B p65,NF-κB p65)通路的影响。方法:建立ROU模型大鼠,将大鼠分为模型组、泼尼松龙(20 mL/kg)组、银翘散低剂量(含生药量16.74 g/kg)、高剂量(含生药量33.48 g/kg)组,另取正常大鼠作为对照组,每组10只,各组大鼠每日进行1次灌胃给药或生理盐水,连续20 d。记录大鼠溃疡情况;苏木精-伊红(hematoxylin-eosin,HE)染色观察病理变化;流式细胞仪检测CD4和CD8含量;生化法和免疫组织化学法检测血清和溃疡组织中肿瘤坏死因子(tumor necrosis factor,TNF)-α、白细胞介素(Interleukin,IL)-6水平;Western blot检测溃疡组织中p-IKKβ、IKKβ、p-NF-κB p65、NF-κBp65蛋白表达。结果:与对照组比较,模型组大鼠溃疡数目,外周血CD8^(+)含量,血清中TNF-α、IL-6水平,溃疡组织中TNF-α、IL-6、p-IKKβ/IKKβ、p-NF-κB p65/NF-κB p65蛋白表达水平均升高(P<0.05);外周血CD4^(+)、CD4^(+)/CD8^(+)(CD4^(+)T细胞/CD8^(+)T细胞)含量降低(P<0.05)。与模型组比较,泼尼松龙组、银翘散低、高剂量组大鼠溃疡数目、持续时间,外周血CD8^(+)含量,血清中TNF-α、IL-6水平,溃疡组织中TNF-α、IL-6、p-IKKβ/IKKβ、p-NF-κB p65/NF-κB p65蛋白表达水平均降低(P<0.05);大鼠间隔时间,外周血CD4^(+)、CD4^(+)/CD8^(+)含量均升高(P<0.05);且银翘散治疗效果呈剂量依赖性(P<0.05)。结论:银翘散可通过抑制ROU大鼠血清和溃疡组织炎症因子表达,调节外周血T细胞亚群平衡,从而促进溃疡愈合,其分子机制可能与下调IKKβ/NF-κB p65信号通路相关。
Objective:To observe the effect of Yinqiao Powder on rats with recurrent oral ulcers(ROU)and to explore its influence on the IκB kinase β subunit(IKKβ)/nuclear factor κB p65 subunit(NF-κB p65)pathway.Methods:A rat model of ROU(recurrent oral ulcers)was established,and the rats were divided into the following groups:model group,prednisolone(20 mL/kg)group,Yinqiao Powder low-dose(16.74 g/kg crude drug)and high-dose(33.48 g/kg crude drug)groups,and normal control group.Each group consisted of 10 rats.The rats in each group were administered either the respective treatment or saline once daily by gavage for 20 consecutive days.The number of ulcers,duration,and interval time in the rats were observed and recorded.Histopathological changes of the ulcer tissue were observed using HE staining.Peripheral blood T lymphocyte subpopulations CD4^(+)and CD8^(+)were measured using flow cytometry.Serum and ulcer tissue levels of tumor necrosis factor-α(TNF-α)and interleukin(IL)-6 were detected using ELISA and immunohistochemistry.The protein expressions of p-IKKβ,IKKβ,p-NF-κB p65,and NF-κB p65 in ulcer tissues were measured using Western blot.Results:Compared with the control group,the model group rats showed increased number of ulcers,peripheral blood CD8^(+)content,serum levels of TNF-α and IL-6,and protein expression levels of TNF-α,IL-6,p-IKKβ/IKKβ,and p-NF-κB p65/NF-κB p65 in ulcer tissues(P<0.05);peripheral blood CD4^(+)and CD4^(+)/CD8^(+)contents were decreased(P<0.05).Compared with the model group,the prednisolone group and the Yinqiao Powder low-and high-dose groups showed reduced number and duration of ulcers,peripheral blood CD8^(+)content,serum levels of TNF-α and IL-6,and protein expression levels of TNF-α,IL-6,p-IKKβ/IKKβ,and p-NF-κB p65/NF-κB p65 in ulcer tissues(P<0.05);the interval time between ulcers and peripheral blood CD4^(+)and CD4^(+)/CD8^(+)contents were increased(P<0.05);and the therapeutic effect of Yinqiao Powder was dose-dependent(P<0.05).Conclusion:Yinqiao Powder can promote ulcer healing by inhibiting the expression of inflammatory factors in serum and ulcer tissues of ROU rats,and regulating the balance of T cell subsets in peripheral blood.Its molecular mechanism may be related to the down-regulation of the IKKβ/NF-κB p65 signaling pathway.
作者
关景平
李霞
李玲
马朝卿
王金星
GUAN Jingping;LI Xia;LI Ling;MA Chaoqing;WANG Jinxing(Department of Stomatology,Hengshui Fourth People's Hospital(Affiliated Hospital of Chengde Medical College),Hengshui 053000,China;Department of Stomatology,Hengshui Fifth People's Hospital,Hengshui 053000,China;Department of Stomatology,Sanhe City Hospital,Langfang 065200,China)
出处
《口腔医学研究》
北大核心
2025年第12期1081-1086,共6页
Journal of Oral Science Research
基金
河北省2022年度中医药类科研计划项目(编号:2022628)。
