Xenocoumacins(Xcns),the major antimicrobial natural products produced by Xenorhabdus nematophila,have gained widespread attention for their potential application in crop protection.However,the regulatory mechanisms in...Xenocoumacins(Xcns),the major antimicrobial natural products produced by Xenorhabdus nematophila,have gained widespread attention for their potential application in crop protection.However,the regulatory mechanisms involved in the biosynthesis of Xcns remain poorly understood.In this study,we identified 21 potential twocomponent systems(TCSs)in X.nematophila CB6 by bioinformatic analysis.Among them,the response regulators(RRs),GlrR and ArcA,were proven to positively regulate the production of Xcns based on gene deletion and complementation experiments.In addition,our results showed that GlrR played an important role in cell growth,while ArcA was involved in both cell morphology and growth.Using a variety of molecular biological and biochemical techniques,we found that GlrR controlled the Xcns biosynthesis by indirectly regulating the expression levels of the biosynthetic gene cluster(BGC).ArcA directly binded to the promoter regions of xcn A and xcn B to regulate the transcription of the Xcns BGC,and the binding sites were also identified.This study provides valuable insights into the regulatory network of Xcns biosynthesis,which will contribute to the construction of a high-yielding strain.展开更多
目的从对植物疫病有拮抗作用的嗜线虫致病杆菌北京变种(X enorhabdus nem a toph ilus var.p ek ing ensis)CB 6菌株的发酵产物中分离纯化抗菌组分,并鉴定其结构。方法采用多种层析方法对其进行分离,并采用UV、IR、M S和NM R对其进行结...目的从对植物疫病有拮抗作用的嗜线虫致病杆菌北京变种(X enorhabdus nem a toph ilus var.p ek ing ensis)CB 6菌株的发酵产物中分离纯化抗菌组分,并鉴定其结构。方法采用多种层析方法对其进行分离,并采用UV、IR、M S和NM R对其进行结构鉴定。结果分离到一个主要组分CB 6-1,并鉴定其结构为3,4-二氢-8-羟基苯并吡喃衍生物。结论主要抗菌组分CB 6-1与文献报道的X encoum ac in 1相同。展开更多
基金support from the National Natural Science Foundation of China(31972327)the Key Research and Development Program Project of Xinjiang,China(2022B02044)+2 种基金the National Key R&D Program of China(2023YFD1700700)the Agricultural Science and Technology Innovation Program of CAASthe Chinese Academy of Agriculture Sciences for the fund of Elite Youth Program。
文摘Xenocoumacins(Xcns),the major antimicrobial natural products produced by Xenorhabdus nematophila,have gained widespread attention for their potential application in crop protection.However,the regulatory mechanisms involved in the biosynthesis of Xcns remain poorly understood.In this study,we identified 21 potential twocomponent systems(TCSs)in X.nematophila CB6 by bioinformatic analysis.Among them,the response regulators(RRs),GlrR and ArcA,were proven to positively regulate the production of Xcns based on gene deletion and complementation experiments.In addition,our results showed that GlrR played an important role in cell growth,while ArcA was involved in both cell morphology and growth.Using a variety of molecular biological and biochemical techniques,we found that GlrR controlled the Xcns biosynthesis by indirectly regulating the expression levels of the biosynthetic gene cluster(BGC).ArcA directly binded to the promoter regions of xcn A and xcn B to regulate the transcription of the Xcns BGC,and the binding sites were also identified.This study provides valuable insights into the regulatory network of Xcns biosynthesis,which will contribute to the construction of a high-yielding strain.
文摘目的从对植物疫病有拮抗作用的嗜线虫致病杆菌北京变种(X enorhabdus nem a toph ilus var.p ek ing ensis)CB 6菌株的发酵产物中分离纯化抗菌组分,并鉴定其结构。方法采用多种层析方法对其进行分离,并采用UV、IR、M S和NM R对其进行结构鉴定。结果分离到一个主要组分CB 6-1,并鉴定其结构为3,4-二氢-8-羟基苯并吡喃衍生物。结论主要抗菌组分CB 6-1与文献报道的X encoum ac in 1相同。
