Introduction: Inappropriate and excess vitamin supplementation, particularly for vitamin A, is increasingly recognized as a public health problem in developed countries. On the other hand, blind supplementation of vit...Introduction: Inappropriate and excess vitamin supplementation, particularly for vitamin A, is increasingly recognized as a public health problem in developed countries. On the other hand, blind supplementation of vitamin A, for children in developing countries is a subject of controversy in the literature. The crucial role of vitamin A in the process of spermatogenesis in adult rodents is well established, but only a few publications are consecrated to the long-term effect of vitamin A intake at a young age on testicular development and differentiation. Objectives: Our study aimed to evaluate the long-term effects of acute supplementation at an early age, in the post-natal period, on spermatogenesis and testicular trophicity at adult age. Material and Methods: Young Wistar Albinos rats of 22 days received an acute high dose of supplementation of vitamin A (retinyl palmitate). The control group, group 1, received only extra virgin olive oil, Group 2 a dose of 7000 IU/kg of retinyl palmitate, group 3, 14,000 IU/kg, and Group 4 a dose of 28,000 IU/kg. At 10 weeks of age, the testes’ testosterone levels were measured by ELISA. For histological assessment, sections were stained with Hematoxylin eosin, and the Johnsen score was used to evaluate spermatogenesis in the seminiferous tubules. Results: The average testicular weights of rats were significantly lower in group 4 (p < 0.05), and so was the testosterone level in the testis compared to the control group (p .01). Most of the seminiferous tubules were concerned by an arrest of spermatogenesis and the Johnsen score was decreased with a mean score of 5.96 ± 1.60 (p .001) in that Group. In Group 3, Johnsen’s score was significantly better than the one obtained with the control. Conclusion: We observed a negative effect in the long term with a high acute dose of supplementation of retinyl palmitate at a young age, on testicular development and differentiation. Despite a return to normal diet after that supplementation, during childhood, impaired spermatogenesis was identified at the adult age with an arrest of spermatogenesis. The reversibility of that lack of differentiation by a return to a normal diet is questionable and would need more investigation.展开更多
The aim of this study was to evaluate the effects of thermooxidation of S. ochocoa oil on its quality and on the biochemical and anthropometric parameters of Wistar rats. S. ochocoa seeds from the Lekoumou department ...The aim of this study was to evaluate the effects of thermooxidation of S. ochocoa oil on its quality and on the biochemical and anthropometric parameters of Wistar rats. S. ochocoa seeds from the Lekoumou department were used for oil extraction at the press. The parameters measured were oil content and chemical indices, determined for samples of oil unheated and heated to 220˚C as a function of the number of heatings and the environment (open air, freezer). Two diets were created: the control diet and the treated diet, in which 10% oil was substituted for the control diet. These diets were fed to the rats for 28 days. Biochemical, anthropometric and atherogenicity parameters were measured. The results show that S. ochocoa oil has a saturated character and a blackish color, and it solidifies or coagulates rapidly. Unheated oil samples placed in a freezer and unheated samples placed in a simple freezer have relatively lower acid values (0.860 and 0.703 mg KOH/g respectively) than other samples. Acid index values for heated samples increase progressively with the number of heatings (1.063 ± 0.005 for 1st heating, 1.290 ± 0.010 for 2nd heating and 1.890 ± 0.006 for 3rd heating). The oil’s peroxide value increased with temperature and oxidation (65.6 ± 0.03 for unheated oil vs. 97.9 ± 003 for oil heated to 220˚C). The diet supplemented with this oil resulted in a significant increase in plasma cholesterol levels, and a significant decrease in triglyceride levels. The plasma atherogenicity index reveals the risk of cardiovascular disease associated with this food.展开更多
文摘Introduction: Inappropriate and excess vitamin supplementation, particularly for vitamin A, is increasingly recognized as a public health problem in developed countries. On the other hand, blind supplementation of vitamin A, for children in developing countries is a subject of controversy in the literature. The crucial role of vitamin A in the process of spermatogenesis in adult rodents is well established, but only a few publications are consecrated to the long-term effect of vitamin A intake at a young age on testicular development and differentiation. Objectives: Our study aimed to evaluate the long-term effects of acute supplementation at an early age, in the post-natal period, on spermatogenesis and testicular trophicity at adult age. Material and Methods: Young Wistar Albinos rats of 22 days received an acute high dose of supplementation of vitamin A (retinyl palmitate). The control group, group 1, received only extra virgin olive oil, Group 2 a dose of 7000 IU/kg of retinyl palmitate, group 3, 14,000 IU/kg, and Group 4 a dose of 28,000 IU/kg. At 10 weeks of age, the testes’ testosterone levels were measured by ELISA. For histological assessment, sections were stained with Hematoxylin eosin, and the Johnsen score was used to evaluate spermatogenesis in the seminiferous tubules. Results: The average testicular weights of rats were significantly lower in group 4 (p < 0.05), and so was the testosterone level in the testis compared to the control group (p .01). Most of the seminiferous tubules were concerned by an arrest of spermatogenesis and the Johnsen score was decreased with a mean score of 5.96 ± 1.60 (p .001) in that Group. In Group 3, Johnsen’s score was significantly better than the one obtained with the control. Conclusion: We observed a negative effect in the long term with a high acute dose of supplementation of retinyl palmitate at a young age, on testicular development and differentiation. Despite a return to normal diet after that supplementation, during childhood, impaired spermatogenesis was identified at the adult age with an arrest of spermatogenesis. The reversibility of that lack of differentiation by a return to a normal diet is questionable and would need more investigation.
文摘The aim of this study was to evaluate the effects of thermooxidation of S. ochocoa oil on its quality and on the biochemical and anthropometric parameters of Wistar rats. S. ochocoa seeds from the Lekoumou department were used for oil extraction at the press. The parameters measured were oil content and chemical indices, determined for samples of oil unheated and heated to 220˚C as a function of the number of heatings and the environment (open air, freezer). Two diets were created: the control diet and the treated diet, in which 10% oil was substituted for the control diet. These diets were fed to the rats for 28 days. Biochemical, anthropometric and atherogenicity parameters were measured. The results show that S. ochocoa oil has a saturated character and a blackish color, and it solidifies or coagulates rapidly. Unheated oil samples placed in a freezer and unheated samples placed in a simple freezer have relatively lower acid values (0.860 and 0.703 mg KOH/g respectively) than other samples. Acid index values for heated samples increase progressively with the number of heatings (1.063 ± 0.005 for 1st heating, 1.290 ± 0.010 for 2nd heating and 1.890 ± 0.006 for 3rd heating). The oil’s peroxide value increased with temperature and oxidation (65.6 ± 0.03 for unheated oil vs. 97.9 ± 003 for oil heated to 220˚C). The diet supplemented with this oil resulted in a significant increase in plasma cholesterol levels, and a significant decrease in triglyceride levels. The plasma atherogenicity index reveals the risk of cardiovascular disease associated with this food.
