Cowpea(Vigna imguicuiata) is an important legume crop with diverse uses. The species is presently a minor crop, and evaluation of its genetic diversity has been very limited. In this study, a total of 200 genic and 10...Cowpea(Vigna imguicuiata) is an important legume crop with diverse uses. The species is presently a minor crop, and evaluation of its genetic diversity has been very limited. In this study, a total of 200 genic and 100 genomic simple sequence repeat(SSR) markers were developed from cowpea unigene and genome sequences, respectively. Among them, 27 genic and 27 genomic SSR markers were polymorphic and were used for assessment of genetic diversity and population structure in 105 selected cowpea accessions. A total of 155 alleles and 2.9 alleles per marker were identified, and the average polymorphic information content(PIC) value was 0.3615. The average PIC of genomic SSRs(0.3996) was higher than that of genic SSRs(0.3235), and most of the polymorphic genomic SSRs were composed of di-and trinucleotide repeats(51.9% and 37.0% of all loci, respectively). The low level of detected genetic diversity may be attributed to a severe genetic bottleneck that occurred during the cowpea domestication process. The accessions were classified by structure and cluster analysis into four subgroups that correlated well with their geographic origins or collection sites. The classification results were also consistent with the results from principal coordinate analysis and can be used as a guide during future germplasm collection and selection of accessions as breeding materials for cultivar improvement. The newly developed genic and genomic SSR markers described in this study will be valuable genomic resources for the assessment of genetic diversity, population structure, evaluation of germplasm accessions, construction of genetic maps, identification of genes of interest,and application of marker-assisted selection in cowpea breeding programs.展开更多
Savanna regions of Nigeria are deficient in nitrogen and phosphorus, which retard the growth and yield of crops. Therefore, a study was conducted in the wet season of 2006 at the Dry Land Teaching and Research Farm of...Savanna regions of Nigeria are deficient in nitrogen and phosphorus, which retard the growth and yield of crops. Therefore, a study was conducted in the wet season of 2006 at the Dry Land Teaching and Research Farm of Usmanu Danfodiyo University, Sokoto to evaluate the effect of phosphorus on the growth and yield of two cowpea varieties sourced from Republic of Niger. Treatment consisted of four (4) rates of phosphorus (0, 20, 40, 60 kg.ha–1) factorialy combined with (2) varieties of cowpea (kvx303096G and TN5-78) and laid out in a randomized complete block design (RCBD) replicated three (3) times. Results showed significant response to applied P on pods per plant, grain and stover yield and 100-seed weight with highest response to the application of 60 kg.P.ha–1. From this study it can be concluded that KVX303096G and TN5-78 could both be sown under Sokoto condition to obtain reasonable yield of about 1 t.ha–1 of grain and 1.6 t.ha–1 of stover. Irrespective of the varieties, application of 60 kg P2O5 ha–1 could be recommended for higher yield of cowpea (1.4 t.ha–1) relative to 0 kg.P.ha–1 that yielded 1.0 t.ha–1.展开更多
Vigna unguiculata L. Walp is a recalcitrant plant in terms of in vitro cell, tissue and organ differentiation, which makes it difficult to apply tissue-culture dependant approaches for obtaining stable genetic transfo...Vigna unguiculata L. Walp is a recalcitrant plant in terms of in vitro cell, tissue and organ differentiation, which makes it difficult to apply tissue-culture dependant approaches for obtaining stable genetic transformation in cowpea. Despite this, sporadic efforts have been made to develop regeneration systems in cowpea during the past 40 years. This review presents the considerable progress on cowpea regeneration （organogenesis and embryogenesis） and especially focuses on the regeneration mode of organogenesis, including highlights of the effect of genotypes, explants, medium and plant hormones used in tissue culture. The existing problems and the future research directions were also discussed.展开更多
Main observation and conclusion An HPLC-UV-guided separation was performed and four pairs of unprecedented macathiohydantoin dimers,lepithiohydimerins A—D(1—4)bearing a rare disulfide bond were isolated from the tub...Main observation and conclusion An HPLC-UV-guided separation was performed and four pairs of unprecedented macathiohydantoin dimers,lepithiohydimerins A—D(1—4)bearing a rare disulfide bond were isolated from the tubers of Maca.Their structures were unambiguously confirmed by NMR spectroscopic,X-ray crystallographic and electronic circular dichroism(ECD)analyses.At the concentration of 20μmol/L,compounds 2-1,2-2,and 4-1 increased the viability of PC12 cells with the cell viability at(72.06±1.14)%,(72.64±1.49)%,and(70.93±1.22)%,respectively.Furthermore,the serial concentration experiment showed that they can protect PC12 cells in a dose-dependent manner.展开更多
基金funded by the Agricultural Science and Technology Innovation Program (ASTIP) of CAASthe China Agriculture Research System (CARS-09)
文摘Cowpea(Vigna imguicuiata) is an important legume crop with diverse uses. The species is presently a minor crop, and evaluation of its genetic diversity has been very limited. In this study, a total of 200 genic and 100 genomic simple sequence repeat(SSR) markers were developed from cowpea unigene and genome sequences, respectively. Among them, 27 genic and 27 genomic SSR markers were polymorphic and were used for assessment of genetic diversity and population structure in 105 selected cowpea accessions. A total of 155 alleles and 2.9 alleles per marker were identified, and the average polymorphic information content(PIC) value was 0.3615. The average PIC of genomic SSRs(0.3996) was higher than that of genic SSRs(0.3235), and most of the polymorphic genomic SSRs were composed of di-and trinucleotide repeats(51.9% and 37.0% of all loci, respectively). The low level of detected genetic diversity may be attributed to a severe genetic bottleneck that occurred during the cowpea domestication process. The accessions were classified by structure and cluster analysis into four subgroups that correlated well with their geographic origins or collection sites. The classification results were also consistent with the results from principal coordinate analysis and can be used as a guide during future germplasm collection and selection of accessions as breeding materials for cultivar improvement. The newly developed genic and genomic SSR markers described in this study will be valuable genomic resources for the assessment of genetic diversity, population structure, evaluation of germplasm accessions, construction of genetic maps, identification of genes of interest,and application of marker-assisted selection in cowpea breeding programs.
文摘Savanna regions of Nigeria are deficient in nitrogen and phosphorus, which retard the growth and yield of crops. Therefore, a study was conducted in the wet season of 2006 at the Dry Land Teaching and Research Farm of Usmanu Danfodiyo University, Sokoto to evaluate the effect of phosphorus on the growth and yield of two cowpea varieties sourced from Republic of Niger. Treatment consisted of four (4) rates of phosphorus (0, 20, 40, 60 kg.ha–1) factorialy combined with (2) varieties of cowpea (kvx303096G and TN5-78) and laid out in a randomized complete block design (RCBD) replicated three (3) times. Results showed significant response to applied P on pods per plant, grain and stover yield and 100-seed weight with highest response to the application of 60 kg.P.ha–1. From this study it can be concluded that KVX303096G and TN5-78 could both be sown under Sokoto condition to obtain reasonable yield of about 1 t.ha–1 of grain and 1.6 t.ha–1 of stover. Irrespective of the varieties, application of 60 kg P2O5 ha–1 could be recommended for higher yield of cowpea (1.4 t.ha–1) relative to 0 kg.P.ha–1 that yielded 1.0 t.ha–1.
文摘Vigna unguiculata L. Walp is a recalcitrant plant in terms of in vitro cell, tissue and organ differentiation, which makes it difficult to apply tissue-culture dependant approaches for obtaining stable genetic transformation in cowpea. Despite this, sporadic efforts have been made to develop regeneration systems in cowpea during the past 40 years. This review presents the considerable progress on cowpea regeneration （organogenesis and embryogenesis） and especially focuses on the regeneration mode of organogenesis, including highlights of the effect of genotypes, explants, medium and plant hormones used in tissue culture. The existing problems and the future research directions were also discussed.
基金supported by grants from the National Natural Science Foundation of China(No.31872675)Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2020-KF02).
文摘Main observation and conclusion An HPLC-UV-guided separation was performed and four pairs of unprecedented macathiohydantoin dimers,lepithiohydimerins A—D(1—4)bearing a rare disulfide bond were isolated from the tubers of Maca.Their structures were unambiguously confirmed by NMR spectroscopic,X-ray crystallographic and electronic circular dichroism(ECD)analyses.At the concentration of 20μmol/L,compounds 2-1,2-2,and 4-1 increased the viability of PC12 cells with the cell viability at(72.06±1.14)%,(72.64±1.49)%,and(70.93±1.22)%,respectively.Furthermore,the serial concentration experiment showed that they can protect PC12 cells in a dose-dependent manner.
