目的研究WWCs(WW and C2 Domain containing protein family)及其各亚型(WWC1,WWC2,WWC3)在人类肺癌中的表达是否存在差异、与临床病理因素的关系及其发挥生物学作用的相关机制。方法应用免疫组化方法检测了159例非小细胞肺癌组织标本中...目的研究WWCs(WW and C2 Domain containing protein family)及其各亚型(WWC1,WWC2,WWC3)在人类肺癌中的表达是否存在差异、与临床病理因素的关系及其发挥生物学作用的相关机制。方法应用免疫组化方法检测了159例非小细胞肺癌组织标本中WWCs蛋白表达和亚细胞定位,并对比分析与上皮间质转化相关蛋白E-cadherin、N-cadherin表达的关系。利用集落形成实验和transwell实验,检测WWC3对肺癌细胞增殖及侵袭能力的影响。转染或干扰WWC3的表达后,通过Western blot检测双向调控WWC3对E-cadherin、N-cadherin及Snail表达水平的影响。结果 WWC3在正常支气管上皮细胞浆中高表达(31/40,77.5%),而在非小细胞肺癌中低表达(70/159,44%),明显低于正常支气管上皮的阳性表达率(P<0.001),且其低表达与非小细胞肺癌的低分化(P=0.010)、高TNM分期(P=0.016)、淋巴结转移(P=0.002)相关。WWC3的低表达与上皮间质转化相关蛋白E-cadherin的高表达(P=0.032)、N-cadherin的低表达(P=0.007)相关。在H1299细胞中转染WWC3表达质粒,抑制肺癌细胞的增殖与侵袭能力,下调Snail和N-cadherin,上调E-cadherin的表达。在A549细胞中转染WWC3-si RNA能够明显促进肺癌细胞的增殖和侵袭能力,上调Snail和N-cadherin的表达,而下调E-cadherin的表达。结论 WWC3抑制肺癌细胞侵袭和转移可能与下调Snail和N-cadherin,上调E-cadherin的表达相关。展开更多
目的探讨WWC3在胶质瘤干细胞(GSCs)中的表达,以及通过调节Hippo通路调控GSCs增殖、迁移和侵袭的作用。方法 Western blot检测Hippo通路相关分子的表达。过表达WWC3后,检测MST1、LATS1和YAP的磷酸化与表达;免疫荧光检测YAP分布变化;CCK8...目的探讨WWC3在胶质瘤干细胞(GSCs)中的表达,以及通过调节Hippo通路调控GSCs增殖、迁移和侵袭的作用。方法 Western blot检测Hippo通路相关分子的表达。过表达WWC3后,检测MST1、LATS1和YAP的磷酸化与表达;免疫荧光检测YAP分布变化;CCK8和Transwell检测GSCs增殖、迁移和侵袭的变化。结果 WWC3、MST1和LATS1在GSCs中的表达均显著降低,而YAP的表达显著升高。过表达WWC3显著增加p-MST1/MST1、p-LATS1/LATS1、p-YAP/YAP的表达水平,抑制细胞核中YAP表达,抑制GSCs的增殖、迁移和侵袭。结论过表达WWC3通过降低Hippo通路活性抑制GSCs的增殖、迁移和侵袭。展开更多
Background:Non-Communicable Disease(NCD)incidence continues to increase globally causing high morbidity and mortality.Despite being a major health problem in Sri Lanka,low attendance is reported in free government NCD...Background:Non-Communicable Disease(NCD)incidence continues to increase globally causing high morbidity and mortality.Despite being a major health problem in Sri Lanka,low attendance is reported in free government NCD screening services(i.e.,Healthy life style center(HLC)and the Well Women Clinic(WWC)).This community based study aims to assess the attendance in NCD screening services,and its associated factors among 35 to 65 year adults in Dimbulagala Medical Officer of Health(MOH)area in Sri Lanka.Methods:The community based cross sectional study interviewed resident adults of the Dimbulagala MOH area in the North Central Province of Sri Lanka during August 2016.The sample of residents(n=575)were selected from 30 housing clusters representing the study setting and an interviewer administered questionnaire was used among residents of both genders to ascertain the data on attendance and factors associated with attendance to NCD screening clinics.Results:Five hundred and thirty-seven(n=537)questionnaires were successfully administered with a response rate of 93%.The mean age of the study population was 46.5(Standard Deviation(SD)=8.75)years.Majority(83.2%)of the study population had scored more than the middle value(i.e.,20)of the knowledge score and only 37.8% individuals have ever attended an NCD screening clinic.The“gender”and“awareness on HLC/WWC”are the most important factors determining the attendance to a screening clinic.Conclusions and recommendations:There is a gap between the respondents’NCD screening knowledge and attendance to NCD screening services.Main factors associated with attending NCD screening clinics were gender of the study participants and their awareness of HLC or WWC.展开更多
文摘目的探讨WWC3在胶质瘤干细胞(GSCs)中的表达,以及通过调节Hippo通路调控GSCs增殖、迁移和侵袭的作用。方法 Western blot检测Hippo通路相关分子的表达。过表达WWC3后,检测MST1、LATS1和YAP的磷酸化与表达;免疫荧光检测YAP分布变化;CCK8和Transwell检测GSCs增殖、迁移和侵袭的变化。结果 WWC3、MST1和LATS1在GSCs中的表达均显著降低,而YAP的表达显著升高。过表达WWC3显著增加p-MST1/MST1、p-LATS1/LATS1、p-YAP/YAP的表达水平,抑制细胞核中YAP表达,抑制GSCs的增殖、迁移和侵袭。结论过表达WWC3通过降低Hippo通路活性抑制GSCs的增殖、迁移和侵袭。
文摘Background:Non-Communicable Disease(NCD)incidence continues to increase globally causing high morbidity and mortality.Despite being a major health problem in Sri Lanka,low attendance is reported in free government NCD screening services(i.e.,Healthy life style center(HLC)and the Well Women Clinic(WWC)).This community based study aims to assess the attendance in NCD screening services,and its associated factors among 35 to 65 year adults in Dimbulagala Medical Officer of Health(MOH)area in Sri Lanka.Methods:The community based cross sectional study interviewed resident adults of the Dimbulagala MOH area in the North Central Province of Sri Lanka during August 2016.The sample of residents(n=575)were selected from 30 housing clusters representing the study setting and an interviewer administered questionnaire was used among residents of both genders to ascertain the data on attendance and factors associated with attendance to NCD screening clinics.Results:Five hundred and thirty-seven(n=537)questionnaires were successfully administered with a response rate of 93%.The mean age of the study population was 46.5(Standard Deviation(SD)=8.75)years.Majority(83.2%)of the study population had scored more than the middle value(i.e.,20)of the knowledge score and only 37.8% individuals have ever attended an NCD screening clinic.The“gender”and“awareness on HLC/WWC”are the most important factors determining the attendance to a screening clinic.Conclusions and recommendations:There is a gap between the respondents’NCD screening knowledge and attendance to NCD screening services.Main factors associated with attending NCD screening clinics were gender of the study participants and their awareness of HLC or WWC.
