One of the most promising vector control strategies for controlling dengue fever is the stable introduction of the obligate intracellular Wolbachia bacteria strain into Aedes aegypti mosquitoes. This method reduces th...One of the most promising vector control strategies for controlling dengue fever is the stable introduction of the obligate intracellular Wolbachia bacteria strain into Aedes aegypti mosquitoes. This method reduces the mosquito’s ability to transmit dengue through reproductive strategies associated with Wolbachia infection, such as parthenogenesis, male elimination or feminization, sex ratio distortions and cytoplasmic incompatibility. Expert knowledge and a risk assessment framework were used to identify the risks associated with the release of Wolbachia-bearing mosquitoes. Then, consultations with individual participants were organized and a Bayesian network (BN) was developed to capture the relationship between the hazards as well as the likelihood of these adverse events occurring. Finally, risk was calculated from the probability and consequence estimates obtained from our Burkina Faso participants, using a questionnaire based on the identified risks. Our “Cause More Harm” study yielded 46.15% negligible risk, 44.23% very low risk and 9.62% low risk. The “socio-cultural change” parameter had the greatest influence, with the perception that the dissemination project would be poorly received by the local population. This parameter alone accounted for 80% of the most significant risks. This explains the fact that hazard RA 49 “negative messages in social media” is ranked as the highest individual risk (although the risk is low) with a calculated risk of 0.261. The risk assessment was designed to integrate the interdependent complexity of hazards likely to affect the dissemination of technology in the environment. It represents an important implementation phase in the success of this innovative research, introducing a new technology to combat the transmission of dengue fever.展开更多
Objective:To identify the efficacy of Wolbachia-based intervention by reviewing community-based trials through meta-analysis and systematic review methods.Methods:Studies about Wolbachia-infected mosquito intervention...Objective:To identify the efficacy of Wolbachia-based intervention by reviewing community-based trials through meta-analysis and systematic review methods.Methods:Studies about Wolbachia-infected mosquito intervention were collected through a screening process.Records underwent data extraction and quality assessment independently by the authors.The primary outcome of the study was protective efficacy.Quantitative analysis was conducted through meta-analysis and multivariate meta-regression using Rstudio Ver.2024.09.0-375.Qualitative analysis was done by summarizing records’findings.Results:10 out of the 10660 records met the criteria.The evidence was moderate in quality and highly heterogeneous.Intervention consisted of releasing Wolbachia-infected Aedes aegypti in densely populated settlements.The combined protective efficacy of Wolbachia intervention against dengue is highly heterogeneous(79%;95%CI 70-88;I^(2)=98%).wMel strain is significantly more efficacious compared to wAlbB(protective efficacy 84%;95%CI 76-93;I^(2)=95%)vs.64%(95%CI 46-82;I^(2)=85%);P<0.01)in preventing dengue cases.Wolbachia-infected mosquito populations were found to be unstable upon release cessation,which necessitated periodic release and monitoring to maintain desired concentration and protective efficacy.Conclusions:Wolbachia-based intervention is effective in suppressing the transmission of mosquito-borne diseases,especially dengue,with an excellent safety profile.However,community acceptance and policy remain as significant barriers to implementation.展开更多
Background:This article examines the impact of the release of Wolbachia-infected population replacement mosquitoes as an innovative strategy for managing and controlling dengue in the Americas,using an approach based ...Background:This article examines the impact of the release of Wolbachia-infected population replacement mosquitoes as an innovative strategy for managing and controlling dengue in the Americas,using an approach based on the system dynamics methodology.The introduction of Wolbachia-carrying mosquitoes aims to reduce dengue transmission by interfering with the reproductive capacity of mosquitoes,thus limiting the spread of the virus.Objective:the objective of this study is to analyze how this intervention affects not only the incidence of dengue but also the health care system,evaluating changes in the demand for medical services and the costs associated with treatment in health care institutions.Specifically,it looks at the cost per visit to an Epidemiologist,a Family Doctor,diagnostic tests,and hospitalization.Methods:the study uses simulation scenarios to model the potential impact of the Wolbachia-based intervention in reducing dengue cases and its associated health and economic burden.The scenarios also assess the optimization of resources and improvements in the health system’s response to epidemic outbreaks.The simulation model utilizes real data from the Americas region to enhance the accuracy and relevance of the results.Conclusion:The results the potential of the release of Wolbachia-carrying mosquitoes to significantly mitigate the health and economic burden of dengue,with a maximum saving of 60.15%in the best simulation scenario.The approach not only highlights scientific innovation but also demonstrates its potential influence on public policy design.The findings support the implementation of sustainable strategies to maximize the benefits of this intervention and ensure its effective integration into public health programs,contributing to better long-term dengue management.展开更多
Due to deficient treatments and vaccines for dengue viruses,dengue control mainly depends on controlling mosquitoes.Suppressing wild mosquito population size by releasing male-only Wolbachia-carrying mosquitoes into t...Due to deficient treatments and vaccines for dengue viruses,dengue control mainly depends on controlling mosquitoes.Suppressing wild mosquito population size by releasing male-only Wolbachia-carrying mosquitoes into the field has become an eco-friendly alternative for traditional mosquito control methods.By assuming an ideal continuous proportional release strategy,we formulate a two-strain vector-host model incorporating larval competition to describe the affected cross-transmission dynamics of two dengue viruses serotypes.Moreover,antibody-dependent enhancement(ADE)and temporal cross-immunity are considered in this model.Theoretical analysis including the well-posedness,the existence and local stability of dengue-free equilibria in terms of the basic reproduction numbers is conducted.Sensitivity analysis indicates that the basic reproduction numbers are most sensitive to the adult female mortality rate and mosquito bite rate.As expected,intensifying larval competition can prevent the spread of dengue viruses.Unexpectedly,increasing the release ratio can delay the peak time while increase the peak level of both primary and secondary infections.To efficiently eliminate dengue outbreaks,other control methods such as spraying larvicides/adult insecticides and avoiding mosquito bites should be employed simultaneously during the time period gained by releasing Wolbachia-carrying males.展开更多
【目的】对茶尺蠖Ectropis obliqua及其近缘种灰茶尺蠖E.grisescens体内共生菌Wolbachia进行分子鉴定,确定两者体内Wolbachia的感染率及其进化地位,为进一步探讨其对茶尺蠖和灰茶尺蠖的潜在影响提供科学依据。【方法】采用Wolbachia的16...【目的】对茶尺蠖Ectropis obliqua及其近缘种灰茶尺蠖E.grisescens体内共生菌Wolbachia进行分子鉴定,确定两者体内Wolbachia的感染率及其进化地位,为进一步探讨其对茶尺蠖和灰茶尺蠖的潜在影响提供科学依据。【方法】采用Wolbachia的16S r RNA、fts Z和wsp基因特异性引物,通过PCR扩增法检测了我国3个茶尺蠖地理种群（浙江杭州、余杭和江苏无锡）和3个灰茶尺蠖地理种群（浙江新昌、湖北浠水和江西南昌）中Wolbachia的感染情况,并进行测序和序列分析。【结果】茶尺蠖和灰茶尺蠖都感染了Wolbachia,灰茶尺蠖的Wolbachia感染率为100%,但茶尺蠖的Wolbachia感染率在22%-95%,且PCR产物电泳得到的条带微弱。wsp序列在茶尺蠖和灰茶尺蠖种间、种内无差异;但16S r RNA序列在茶尺蠖和灰茶尺蠖种间、种内差异为0.362%-0.727%之间;茶尺蠖样本未成功扩增出fts Z序列,灰茶尺蠖样本获得2条fts Z基因序列差异为1.647%。基于Wolbachia的16S r RNA和wsp基因构建的系统发育树表明,本研究中茶尺蠖和灰茶尺蠖种群所感染的Wolbachia全部属于B组的Pip亚组。【结论】茶尺蠖和灰茶尺蠖均被B组Pip亚组的Wolbachia感染,但感染率相差很大,这为研究Wolbachia对茶尺蠖和灰茶尺蠖生物学及生态学的影响奠定了基础。展开更多
文摘One of the most promising vector control strategies for controlling dengue fever is the stable introduction of the obligate intracellular Wolbachia bacteria strain into Aedes aegypti mosquitoes. This method reduces the mosquito’s ability to transmit dengue through reproductive strategies associated with Wolbachia infection, such as parthenogenesis, male elimination or feminization, sex ratio distortions and cytoplasmic incompatibility. Expert knowledge and a risk assessment framework were used to identify the risks associated with the release of Wolbachia-bearing mosquitoes. Then, consultations with individual participants were organized and a Bayesian network (BN) was developed to capture the relationship between the hazards as well as the likelihood of these adverse events occurring. Finally, risk was calculated from the probability and consequence estimates obtained from our Burkina Faso participants, using a questionnaire based on the identified risks. Our “Cause More Harm” study yielded 46.15% negligible risk, 44.23% very low risk and 9.62% low risk. The “socio-cultural change” parameter had the greatest influence, with the perception that the dissemination project would be poorly received by the local population. This parameter alone accounted for 80% of the most significant risks. This explains the fact that hazard RA 49 “negative messages in social media” is ranked as the highest individual risk (although the risk is low) with a calculated risk of 0.261. The risk assessment was designed to integrate the interdependent complexity of hazards likely to affect the dissemination of technology in the environment. It represents an important implementation phase in the success of this innovative research, introducing a new technology to combat the transmission of dengue fever.
文摘Objective:To identify the efficacy of Wolbachia-based intervention by reviewing community-based trials through meta-analysis and systematic review methods.Methods:Studies about Wolbachia-infected mosquito intervention were collected through a screening process.Records underwent data extraction and quality assessment independently by the authors.The primary outcome of the study was protective efficacy.Quantitative analysis was conducted through meta-analysis and multivariate meta-regression using Rstudio Ver.2024.09.0-375.Qualitative analysis was done by summarizing records’findings.Results:10 out of the 10660 records met the criteria.The evidence was moderate in quality and highly heterogeneous.Intervention consisted of releasing Wolbachia-infected Aedes aegypti in densely populated settlements.The combined protective efficacy of Wolbachia intervention against dengue is highly heterogeneous(79%;95%CI 70-88;I^(2)=98%).wMel strain is significantly more efficacious compared to wAlbB(protective efficacy 84%;95%CI 76-93;I^(2)=95%)vs.64%(95%CI 46-82;I^(2)=85%);P<0.01)in preventing dengue cases.Wolbachia-infected mosquito populations were found to be unstable upon release cessation,which necessitated periodic release and monitoring to maintain desired concentration and protective efficacy.Conclusions:Wolbachia-based intervention is effective in suppressing the transmission of mosquito-borne diseases,especially dengue,with an excellent safety profile.However,community acceptance and policy remain as significant barriers to implementation.
文摘Background:This article examines the impact of the release of Wolbachia-infected population replacement mosquitoes as an innovative strategy for managing and controlling dengue in the Americas,using an approach based on the system dynamics methodology.The introduction of Wolbachia-carrying mosquitoes aims to reduce dengue transmission by interfering with the reproductive capacity of mosquitoes,thus limiting the spread of the virus.Objective:the objective of this study is to analyze how this intervention affects not only the incidence of dengue but also the health care system,evaluating changes in the demand for medical services and the costs associated with treatment in health care institutions.Specifically,it looks at the cost per visit to an Epidemiologist,a Family Doctor,diagnostic tests,and hospitalization.Methods:the study uses simulation scenarios to model the potential impact of the Wolbachia-based intervention in reducing dengue cases and its associated health and economic burden.The scenarios also assess the optimization of resources and improvements in the health system’s response to epidemic outbreaks.The simulation model utilizes real data from the Americas region to enhance the accuracy and relevance of the results.Conclusion:The results the potential of the release of Wolbachia-carrying mosquitoes to significantly mitigate the health and economic burden of dengue,with a maximum saving of 60.15%in the best simulation scenario.The approach not only highlights scientific innovation but also demonstrates its potential influence on public policy design.The findings support the implementation of sustainable strategies to maximize the benefits of this intervention and ensure its effective integration into public health programs,contributing to better long-term dengue management.
基金supported by the National Natural Science Foundation of China(Grant No.11901247)Foundation for High-Level Entrepreneurial and Innovative Talents of Jiangsu Province,and Research Grants for High-Level Talents of Jiangsu UniversityJun Wang was supported by NSFC of China(12371114)and National KeyR&D Program of China(2022YFA1005601).
文摘Due to deficient treatments and vaccines for dengue viruses,dengue control mainly depends on controlling mosquitoes.Suppressing wild mosquito population size by releasing male-only Wolbachia-carrying mosquitoes into the field has become an eco-friendly alternative for traditional mosquito control methods.By assuming an ideal continuous proportional release strategy,we formulate a two-strain vector-host model incorporating larval competition to describe the affected cross-transmission dynamics of two dengue viruses serotypes.Moreover,antibody-dependent enhancement(ADE)and temporal cross-immunity are considered in this model.Theoretical analysis including the well-posedness,the existence and local stability of dengue-free equilibria in terms of the basic reproduction numbers is conducted.Sensitivity analysis indicates that the basic reproduction numbers are most sensitive to the adult female mortality rate and mosquito bite rate.As expected,intensifying larval competition can prevent the spread of dengue viruses.Unexpectedly,increasing the release ratio can delay the peak time while increase the peak level of both primary and secondary infections.To efficiently eliminate dengue outbreaks,other control methods such as spraying larvicides/adult insecticides and avoiding mosquito bites should be employed simultaneously during the time period gained by releasing Wolbachia-carrying males.
文摘【目的】对茶尺蠖Ectropis obliqua及其近缘种灰茶尺蠖E.grisescens体内共生菌Wolbachia进行分子鉴定,确定两者体内Wolbachia的感染率及其进化地位,为进一步探讨其对茶尺蠖和灰茶尺蠖的潜在影响提供科学依据。【方法】采用Wolbachia的16S r RNA、fts Z和wsp基因特异性引物,通过PCR扩增法检测了我国3个茶尺蠖地理种群（浙江杭州、余杭和江苏无锡）和3个灰茶尺蠖地理种群（浙江新昌、湖北浠水和江西南昌）中Wolbachia的感染情况,并进行测序和序列分析。【结果】茶尺蠖和灰茶尺蠖都感染了Wolbachia,灰茶尺蠖的Wolbachia感染率为100%,但茶尺蠖的Wolbachia感染率在22%-95%,且PCR产物电泳得到的条带微弱。wsp序列在茶尺蠖和灰茶尺蠖种间、种内无差异;但16S r RNA序列在茶尺蠖和灰茶尺蠖种间、种内差异为0.362%-0.727%之间;茶尺蠖样本未成功扩增出fts Z序列,灰茶尺蠖样本获得2条fts Z基因序列差异为1.647%。基于Wolbachia的16S r RNA和wsp基因构建的系统发育树表明,本研究中茶尺蠖和灰茶尺蠖种群所感染的Wolbachia全部属于B组的Pip亚组。【结论】茶尺蠖和灰茶尺蠖均被B组Pip亚组的Wolbachia感染,但感染率相差很大,这为研究Wolbachia对茶尺蠖和灰茶尺蠖生物学及生态学的影响奠定了基础。
