Ningxian 2A is a new Indica CMS line with WA type sterile cytoplasm in rice. It was developed from the testcross between WA type indica CMS line Jin 23A and the single plant selected from the F_4 population of the cro...Ningxian 2A is a new Indica CMS line with WA type sterile cytoplasm in rice. It was developed from the testcross between WA type indica CMS line Jin 23A and the single plant selected from the F_4 population of the cross Wuxiang 2B×Jin 23B followed by successive backcross over years. In September, 2014, it was technically identified by Jiangsu Provincial Crop Variety Appraisal Committee. Ningxian 2A has the characteristics of complete pollen sterility, high outcrossing rate, good plant type, high combining ability and good grain quality. Among its hybrids, Ningxian you 8（ Ningxian 2A×R08） was registered and released for commercial production in Jiangsu Province in May, 2015.展开更多
The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon Griff.is used widely in three-line indica hybrids.The identification and mapping of restorer of fertility(Rf...The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon Griff.is used widely in three-line indica hybrids.The identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type hybrids.Here we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type CMS.We developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting rates.Genetic analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were predicted.Also,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene WA352.These results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines.展开更多
基金Supported by Jiangsu Agricultural Science and Technology Independent Innovation Fund(CX[12]1003)Jiangsu Science and Technology Support Program(BE2013301)Earmarked Fund for China Agriculture Research System(CARS-01-47)~~
文摘Ningxian 2A is a new Indica CMS line with WA type sterile cytoplasm in rice. It was developed from the testcross between WA type indica CMS line Jin 23A and the single plant selected from the F_4 population of the cross Wuxiang 2B×Jin 23B followed by successive backcross over years. In September, 2014, it was technically identified by Jiangsu Provincial Crop Variety Appraisal Committee. Ningxian 2A has the characteristics of complete pollen sterility, high outcrossing rate, good plant type, high combining ability and good grain quality. Among its hybrids, Ningxian you 8（ Ningxian 2A×R08） was registered and released for commercial production in Jiangsu Province in May, 2015.
基金financially supported by the National Natural Science Foundation of China(31771913,3207150123)the Jiangsu Key R&D Plan(BE2021301-1)+2 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Open Funds of Jiangsu Key Laboratory of Crop Genetics and Physiology(NYCSL201904)the Open Funds of the Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding(PL202004)。
文摘The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon Griff.is used widely in three-line indica hybrids.The identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type hybrids.Here we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type CMS.We developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting rates.Genetic analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were predicted.Also,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene WA352.These results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines.
