The effect of local habitat conditions on organisms, including environmentally-induced morphological changes, constitutes an important aspect of macroecology and evolution. The degree of geographic intraspecific varia...The effect of local habitat conditions on organisms, including environmentally-induced morphological changes, constitutes an important aspect of macroecology and evolution. The degree of geographic intraspecific variation in body dimensions, corporeal ratios and scalation pattern among male and female Palestine vipers(Vipera palaestinae) in Israel were examined. Univariate and multivariate analyses using 20 variable features relating to metric and meristic characters were applied in order to determine the existence of geographic variability in this species. Univariate analysis revealed that the majority of morphological characters possess relatively minor interregional distinctions, with only a few traits demonstrating significant differences. Discriminant analysis of mixed-gender samples using a combination of variables did not distinguish between geographic groups within each sex. The multifactor approach slightly differentiated between samples when sexes were compared separately, but with much overlap. The continuous sampling method revealed no statistically significant relationship between geographic and metric variables across the distribution range. A weak latitudinal cline was observed in snout-vent length, with both sexes being larger in the south. Noticeable temperature-correlated intraspecific variability was found in both body and tail scale counts but not in head scalation features. Generally, both males and females demonstrate the same phenotype-environment correlation. The spatial variations in external morphology suggest that temperature conditions during early ontogenesis may induce quantitative changes in the scalation pattern of V. palaestinae.展开更多
The climatic preferences of Pelias barani, a rare Pontic endemic viper, are analysed and a new locality record is reported. According to species distribution modeling, with the average test AUC was was 0.904 ± 0....The climatic preferences of Pelias barani, a rare Pontic endemic viper, are analysed and a new locality record is reported. According to species distribution modeling, with the average test AUC was was 0.904 ± 0.068, bioclimatic variables such as Precipitation of driest month（45.5%）, Temperature seasonality（18.9%）, Precipitation seasonality（17.9%） and Maximum temperature of warmest period（14.7%） appear to have the most useful information on geographic distribution of Pelias barani. Distribution models of Pelias barani under current climatic conditions showed better adaptation to the northwest and northeast part of Turkey.展开更多
目的从广西圆斑蝰蛇毒中分离纯化凝血因子X激活剂(factor X activator of Russell’sviper venom,RVV-X)并研究其止血作用。方法应用阴离子交换色谱和凝胶过滤从广西圆斑蝰蛇毒中分离纯化RVV-X,以复钙化时间进行活性鉴定,以SDS-PAGE测...目的从广西圆斑蝰蛇毒中分离纯化凝血因子X激活剂(factor X activator of Russell’sviper venom,RVV-X)并研究其止血作用。方法应用阴离子交换色谱和凝胶过滤从广西圆斑蝰蛇毒中分离纯化RVV-X,以复钙化时间进行活性鉴定,以SDS-PAGE测定纯度和分子量。观察RVV-X对家兔肝脏创伤出血模型出血时间及出血量,家兔和肝素化小鼠凝血时间的影响。结果广西圆斑蝰粗毒经阴离子交换色谱获得六个蛋白峰,将RVV-X所在第V峰用凝胶过滤获得的组分A为RVV-X纯品,其凝血活性具有钙依赖性。SDS-PAGE非还原性条件下呈单一蛋白条带,分子量约为97.8kDa,还原性条件下呈二蛋白条带,为一条重链(约76.3kDa)和一条轻链(约21.5kDa)。RVV-X可显著减少家兔肝脏切口的失血量,缩短切口出血时间;能显著缩短家兔及肝素化小鼠凝血时间。结论用层析方法从广西圆斑蝰蛇毒中分离得到的凝血因子X激活剂具有较强的止血活性。展开更多
文摘The effect of local habitat conditions on organisms, including environmentally-induced morphological changes, constitutes an important aspect of macroecology and evolution. The degree of geographic intraspecific variation in body dimensions, corporeal ratios and scalation pattern among male and female Palestine vipers(Vipera palaestinae) in Israel were examined. Univariate and multivariate analyses using 20 variable features relating to metric and meristic characters were applied in order to determine the existence of geographic variability in this species. Univariate analysis revealed that the majority of morphological characters possess relatively minor interregional distinctions, with only a few traits demonstrating significant differences. Discriminant analysis of mixed-gender samples using a combination of variables did not distinguish between geographic groups within each sex. The multifactor approach slightly differentiated between samples when sexes were compared separately, but with much overlap. The continuous sampling method revealed no statistically significant relationship between geographic and metric variables across the distribution range. A weak latitudinal cline was observed in snout-vent length, with both sexes being larger in the south. Noticeable temperature-correlated intraspecific variability was found in both body and tail scale counts but not in head scalation features. Generally, both males and females demonstrate the same phenotype-environment correlation. The spatial variations in external morphology suggest that temperature conditions during early ontogenesis may induce quantitative changes in the scalation pattern of V. palaestinae.
文摘The climatic preferences of Pelias barani, a rare Pontic endemic viper, are analysed and a new locality record is reported. According to species distribution modeling, with the average test AUC was was 0.904 ± 0.068, bioclimatic variables such as Precipitation of driest month（45.5%）, Temperature seasonality（18.9%）, Precipitation seasonality（17.9%） and Maximum temperature of warmest period（14.7%） appear to have the most useful information on geographic distribution of Pelias barani. Distribution models of Pelias barani under current climatic conditions showed better adaptation to the northwest and northeast part of Turkey.
文摘目的从广西圆斑蝰蛇毒中分离纯化凝血因子X激活剂(factor X activator of Russell’sviper venom,RVV-X)并研究其止血作用。方法应用阴离子交换色谱和凝胶过滤从广西圆斑蝰蛇毒中分离纯化RVV-X,以复钙化时间进行活性鉴定,以SDS-PAGE测定纯度和分子量。观察RVV-X对家兔肝脏创伤出血模型出血时间及出血量,家兔和肝素化小鼠凝血时间的影响。结果广西圆斑蝰粗毒经阴离子交换色谱获得六个蛋白峰,将RVV-X所在第V峰用凝胶过滤获得的组分A为RVV-X纯品,其凝血活性具有钙依赖性。SDS-PAGE非还原性条件下呈单一蛋白条带,分子量约为97.8kDa,还原性条件下呈二蛋白条带,为一条重链(约76.3kDa)和一条轻链(约21.5kDa)。RVV-X可显著减少家兔肝脏切口的失血量,缩短切口出血时间;能显著缩短家兔及肝素化小鼠凝血时间。结论用层析方法从广西圆斑蝰蛇毒中分离得到的凝血因子X激活剂具有较强的止血活性。
