摘要
对四种不同来源的RVV对ADP、Col和Ris诱导的血小板聚集抑制作用进行比较研究。RVV-B,RVVS和RVV-Styp对三种诱导剂的血小板聚集均有抑制作用,而RVV-T虽有ADP和Col抑制作用,但缺乏对Ris的抑制活性。预先与RVV孵育的血小板经洗涤后重新悬浮于自身PPP中,与RVV-StyP孵育的血小板完全恢复对Ris的凝集反应,而与RVV-B和RVV-S孵育的血小板仅部分恢复其反应。上述结果表明,在RVV中可能含有二种不同的血小板聚集抑制活性,而其抑制机理可能牵涉到血小板、血浆等因素。
The inhibition of RVV from different source on platelet aggregation induced by ADP, Collagen or Ristocetin were studied. RVV-B, RVV-S and RVV-Styp could inhibited aggregation induced by above reagents. RVV-T could inhibited aggregation induced by ADP and Collagen, but not by Ristocetin. Platelets preincubated with RVV were resuspended in PPP after washed. It was observed that platelets preincubated with RVV-Styp could entirely restored to respond to Ristocetin, but only partially with RVV-B and RVV-S.
These results shown that two distinct platelet inhibitory activities in RVV exist, the mechanism of the inhibition could involved platelet, plasma and other factors.
关键词
圆斑蝰蛇蛇毒
血小板聚集
Vipera russelli venom, Inhibitory action of platelet aggregation
