Porcine reproductive and respiratory syndrome(PRRS) is characterized by reproductive failures in sows and respiratory diseases in pigs of all ages. PRRS virus(PRRSV) is its causative agent and has caused huge economic...Porcine reproductive and respiratory syndrome(PRRS) is characterized by reproductive failures in sows and respiratory diseases in pigs of all ages. PRRS virus(PRRSV) is its causative agent and has caused huge economic losses in the swine industry. Porcine sialoadhesin(pSn) is a putative receptor of PRRSV. Previous studies have shown that a pSn V-set Ig-like domain is signi ficant in PRRSV infection. However, its structural details are not fully known, hindering our deep understanding of PRRSV infection. In this study, we successfully cloned, expressed and puri fied the p Sn V-set Ig-like domain in Drosophila S2 cells. Then we tried to crystallize the target protein and predicted its structure. This will establish the foundation for the further structural study of p Sn, deepen our understanding of the invasion mechanism of PRRSV,and support the structural information for the development of clinical drugs and vaccines against PRRSV.展开更多
传统指令集架构(Instruction Set Architecturem,ISA)因为高昂的版权、指令复杂性和兼容性的问题,越来越限制了计算机技术广泛应用。而精简指令集计算-V(Reduced Instruction Set Computer-Five,RISC-V)是一种全新的免费开源指令集,因...传统指令集架构(Instruction Set Architecturem,ISA)因为高昂的版权、指令复杂性和兼容性的问题,越来越限制了计算机技术广泛应用。而精简指令集计算-V(Reduced Instruction Set Computer-Five,RISC-V)是一种全新的免费开源指令集,因其开放性、灵活性和高效性受到广泛关注。通过对比分析,我们揭示了RISC-V与传统指令集的区别,系统回顾了RISC-V指令集的发展历程及主要特性,重点探讨了其在不同领域中的强大潜力与广发的应用价值。展开更多
基金supported by the National Natural Science Foundation of China (31490601)the National Program on the Key Basic Research Project (2014CB542702)the Basic and Advanced Technology Research Program of Henan Province (162300410252)
文摘Porcine reproductive and respiratory syndrome(PRRS) is characterized by reproductive failures in sows and respiratory diseases in pigs of all ages. PRRS virus(PRRSV) is its causative agent and has caused huge economic losses in the swine industry. Porcine sialoadhesin(pSn) is a putative receptor of PRRSV. Previous studies have shown that a pSn V-set Ig-like domain is signi ficant in PRRSV infection. However, its structural details are not fully known, hindering our deep understanding of PRRSV infection. In this study, we successfully cloned, expressed and puri fied the p Sn V-set Ig-like domain in Drosophila S2 cells. Then we tried to crystallize the target protein and predicted its structure. This will establish the foundation for the further structural study of p Sn, deepen our understanding of the invasion mechanism of PRRSV,and support the structural information for the development of clinical drugs and vaccines against PRRSV.
文摘传统指令集架构(Instruction Set Architecturem,ISA)因为高昂的版权、指令复杂性和兼容性的问题,越来越限制了计算机技术广泛应用。而精简指令集计算-V(Reduced Instruction Set Computer-Five,RISC-V)是一种全新的免费开源指令集,因其开放性、灵活性和高效性受到广泛关注。通过对比分析,我们揭示了RISC-V与传统指令集的区别,系统回顾了RISC-V指令集的发展历程及主要特性,重点探讨了其在不同领域中的强大潜力与广发的应用价值。
